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All Journal Indonesian Journal of Tropical and Infectious Disease Jurnal Kesmas dan Gizi (JKG) Eduvest - Journal of Universal Studies
Mayasari, Evita
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Aerospace Engineering Computer Science & IT Earth & Planetary Sciences Health Professions Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Direct PCR for Escherichia coli: a straightforward and cost-effective method Harahap, Desy Aryani; Yamamoto, Zulham; Widjaja, Sry Suryani; Mayasari, Evita
JURNAL KESMAS DAN GIZI (JKG) Vol. 7 No. 2 (2025): Jurnal Kesmas dan Gizi (JKG)
Publisher : Fakultas Kesehatan Masyarakat Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jkg.v7i2.2606

Abstract

This study compares the direct PCR method with standard PCR to detect Escherichia coli. Escherichia coli is the most widely used organism in biology. Detection of E. coli in water and food is routinely performed, utilizing methods like PCR. The initial stage of PCR preparation involves DNA extraction, which requires a commercial kit. Consequently, this extraction process incurs additional expenses, time, and labor. Therefore, an alternative method is needed, such as direct PCR, which can circumvent the need for DNA extraction. The PCR process facilitates lysis of the bacterial cell wall, releasing nucleic acids, which can then be amplified by Taq polymerase. For the PCR procedure, two groups were formed, each comprising three replicates of the reaction with different DNA templates. The first group utilized a direct culture of Escherichia coli, while the second group incorporated the extracted DNA of Escherichia coli. Our study successfully amplified the metH gene of Escherichia coli without DNA extraction. Electrophoresis analysis revealed that the direct PCR product, sized at 300 bp, appeared more pronounced than the standard PCR product. The findings of this research demonstrated direct PCR as an alternative for detecting Escherichia coli, which would lead to reductions in costs, time, and labor.
The Relationship Between Knowledge and the Utilization of the Maternal and Child Health (MCH) Book Among Pregnant Women at Tanjung Rejo Public Health Center, Deli Serdang Regency Hamonangan Sihombing, Rahmat Jody; Sukatendel, Khairani; Rangkuti, Deske Muhadi; Mayasari, Evita; Hasiana Siburian, Miranda Olivia
Eduvest - Journal of Universal Studies Vol. 5 No. 11 (2025): Eduvest - Journal of Universal Studies
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/eduvest.v5i11.52192

Abstract

Maternal Mortality (MMR) is still a global health problem that needs serious attention. In 2022, Deli Serdang Regency was recorded to have the highest AKI in North Sumatra Province, which was 16 per 42,363 live births. One of the efforts that can be made to reduce this number is by using the Maternal and Child Health Book (KIA). This study aims to analyze the relationship between the level of knowledge of pregnant women and the use of KIA books at the Tanjung Rejo Health Center, Deli Serdang Regency. This study uses an analytical observational design with a cross-sectional approach. A total of 100 pregnant women became respondents to the study. Data was collected through questionnaires, then analyzed using the Spearman statistical test. The use of KIA books is assessed through indicators of readability, comprehension, and application of information, while mother's knowledge is measured through the level of mastery of the content of KIA books. The results showed that the majority of pregnant women were at healthy reproductive age (55%), poorly educated (72%), unemployed (89%), and had high parity (56%). Most respondents (73%) fall into the category of poor utilization of KIA books, while 79% have a low level of knowledge. Statistical analysis showed a significant relationship between the level of knowledge of pregnant women and the use of KIA books (p = 0.001). The use of KIA books in pregnant women is significantly influenced by the level of knowledge.
Antibacterial Effects of Cranberry and Lime Extracts Against Uropathogenic Escherichia coli (UPEC). Nasution, Afrida Aryani; Mayasari, Evita; Simatupang, Maria Magdalena; Amelia, Sri; Alona, Ivana
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.64056

Abstract

Urinary tract infections (UTIs) continue to increase in the number of cases and death rates every year. This increase in the number of deaths is due to an increasing trend in UTI cases in elderly patients. In Indonesia, UTI is generally caused by the uropathogenic Escherichia coli (UPEC) strain. The existence of resistant bacterial infections also triggers increased mortality in UTI patients. Several types of herbs, such as cranberries, are often used traditionally to treat UTI symptoms. Another herb that is also known to have antibacterial effects is lime. This experimental study examined the in vitro antimicrobial effects of ethanol extract of cranberry (Vaccinium macrocarpon) and ethanol extract of lime (Citrus aurantifolia L.) against UPEC by the Kirby-Bauer disc diffusion method. This study used a clinical sample obtained from an outpatient diagnosed with UTI in the Prof. dr. Chairuddin P. Lubis Universitas Sumatera Utara Hospital in March 2023. The UPEC strain was isolated and identified using the Vitek 2 Compact automated system at a local university hospital. The antimicrobial susceptibility test results indicated that the ethanol extract of cranberry did not produce an inhibition zone at concentrations of 12.5%, 25%, 50%, 60%, 70%, and 96%. The ethanol extract of lime demonstrated inhibitory zones against UPEC, with a mean diameter of 12.01 mm at a concentration of 12.5% and a mean diameter of 15.62 mm at 96%. The cranberry extract did not demonstrate an inhibitory zone against UPEC in vitro, whereas the lime extract did.
Co-Authors Alona, Ivana Hamonangan Sihombing, Rahmat Jody Harahap, Desy Aryani Hasiana Siburian, Miranda Olivia Nasution, Afrida Aryani Rangkuti, Deske Muhadi Simatupang, Maria Magdalena Sri Amelia, Sri Sukatendel, Khairani Widjaja, Sry Suryani Yamamoto, Zulham