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All Journal Indonesian Journal of Tropical and Infectious Disease Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Simatupang, Maria Magdalena
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Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Knowledge and Preventive Behaviour Regarding Diarrheagenic Escherichia Coli Infection Amongst Final-Year Medical Students in Universitas Sumatera Utara Eklesia, Maryori; Simatupang, Maria Magdalena
Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Vol. 6 No. 3 (2024): Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Publisher : TALENTA Publisher, Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jetromi.v6i3.15303

Abstract

Background: Escherichia coli, a gram-negative facultative anaerobe bacterium is one of the most important etiologies of acute diarrhea, still a serious public health problem and cause of morbidity and mortality in infants, children, the elderly, and people with immune deficiency. The source of contamination is through the consumption of contaminated food, improperly processed dairy and agricultural products, and contact with people who have poor hygiene. The purpose of the study is to determine the level of knowledge and preventive behavior regarding Diarrheagenic Escherichia coli infection. Method: The type of research in this study is descriptive. The research was conducted by collecting data using a Level of knowledge and preventive behavior regarding the diarrheagenic Escherichia coli infection questionnaire which will be given to respondents. Result: The results were obtained from 100 people. For the level of knowledge, there were 64 (64%) people who had good knowledge, 29 (29%) moderate people, and 7 (7%) low people. For the level of preventive behavior, 81 (81%) people of good behavior were found, 18 (18%) people were moderate and 1 (1%) were low. Conclusion: The majority of final-year medical students of Universitas Sumatera Utara are in a good category both in knowledge and preventive behavior regarding Diarrheagenic Escherichia coli.  
Antimicrobial Activity of the Ethanolic Extract of Durio zibethinus Rind against ESBL-producing Escherichia coli and Klebsiella pneumoniae : Antimicrobial Activity of the Ethanolic Extract of Durian Rind against ESBL-producing E. coli and K. pneumoniae Kannady, Johnson; Mayasari, Evita; Mardiani, Tengku Helvi; Hutagalung, Sunna Vyatra; Alona, Ivana; Simatupang, Maria Magdalena
Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI) Vol. 7 No. 1 (2025): Journal of Endocrinology, Tropical Medicine, and Infectious Disease (JETROMI)
Publisher : TALENTA Publisher, Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jetromi.v7i1.19027

Abstract

Background: Durio zibethinus (durian) contains secondary metabolites with antibacterial potency, such as alkaloids, flavonoids, saponins, tannins, and glycosides. Indonesia is one of the world’s largest producing countries of durian, yet the thorny part of the fruit rind often ends up as waste. Investigations of durian rind for its antimicrobial activity will maximize the benefits and prevent waste accumulation. Objective: In this experimental study, we analyzed the antimicrobial activity of ethanolic extract of durian rind against extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and K. pneumoniae in vitro. Methods: This study used a one-group posttest-only design. The disc diffusion method for antimicrobial susceptibility test was used to test the ethanolic extract of durian rind against ESBL-producing E. coli and K. pneumoniae, each with six experimental repetitions to observe the inhibition zone produced around discs containing the extracts at various concentrations (12.5%, 25%, 50%, 75%, 80%, and 100%). Results: Ethanolic extract of durian rind showed an inhibition zone against ESBL-producing E. coli at the extract concentration of 50%, with an average inhibition zone of 3.57 mm. The inhibition zone was also observed against ESBL-producing K. pneumoniae at the extract concentration of 75%, with an average inhibition zone of 3.73 mm. Conclusions: The ethanolic extract of durian rind showed inhibition zones against ESBL-producing E. coli and K. pneumoniae, showing its potential antimicrobial activity in vitro.
Antibacterial Effects of Cranberry and Lime Extracts Against Uropathogenic Escherichia coli (UPEC). Nasution, Afrida Aryani; Mayasari, Evita; Simatupang, Maria Magdalena; Amelia, Sri; Alona, Ivana
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.64056

Abstract

Urinary tract infections (UTIs) continue to increase in the number of cases and death rates every year. This increase in the number of deaths is due to an increasing trend in UTI cases in elderly patients. In Indonesia, UTI is generally caused by the uropathogenic Escherichia coli (UPEC) strain. The existence of resistant bacterial infections also triggers increased mortality in UTI patients. Several types of herbs, such as cranberries, are often used traditionally to treat UTI symptoms. Another herb that is also known to have antibacterial effects is lime. This experimental study examined the in vitro antimicrobial effects of ethanol extract of cranberry (Vaccinium macrocarpon) and ethanol extract of lime (Citrus aurantifolia L.) against UPEC by the Kirby-Bauer disc diffusion method. This study used a clinical sample obtained from an outpatient diagnosed with UTI in the Prof. dr. Chairuddin P. Lubis Universitas Sumatera Utara Hospital in March 2023. The UPEC strain was isolated and identified using the Vitek 2 Compact automated system at a local university hospital. The antimicrobial susceptibility test results indicated that the ethanol extract of cranberry did not produce an inhibition zone at concentrations of 12.5%, 25%, 50%, 60%, 70%, and 96%. The ethanol extract of lime demonstrated inhibitory zones against UPEC, with a mean diameter of 12.01 mm at a concentration of 12.5% and a mean diameter of 15.62 mm at 96%. The cranberry extract did not demonstrate an inhibitory zone against UPEC in vitro, whereas the lime extract did.
Co-Authors Alona, Ivana Eklesia, Maryori Evita Mayasari Hutagalung, Sunna Vyatra Kannady, Johnson Mardiani, Tengku Helvi Mayasari, Evita Nasution, Afrida Aryani Sri Amelia, Sri