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Journal : Biology, Medicine,

Morpho-Anatomical Analysis of Cosmostigma racemosum (Asclepiadoideae) Flowers Widodo Widodo; Mohamad Amin; Mimien Henie Irawati Al-Muhdar; Muhammad Ja’far Luthfi
Biology, Medicine, & Natural Product Chemistry Vol 3, No 1 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1600.439 KB) | DOI: 10.14421/biomedich.2014.31.35-46

Abstract

Cosmostigma racemosum is a plant species belonging to the family Apocynaceae, the subfamily Asclepiadoideae. Cosmostigma racemosum is found in Nglanggeran Mountain Gunungkidul, Yogyakarta. The local name and its original distribution are not known. Information or study of Cosmostigma racemosum in Indonesia is not available. Comprehensive characterization of this species is important for authentication and addition of data base. Characterization was conducted by analyzing the morphology and anatomy of flower. The objectives of this study were to describe and analyze the morphology and anatomy of C. racemosum flowers.  The method of research was based on observation method and exploration of plant systematics evidence or taxonomy evidence, including analyses and description of morphology and anatomy of flower structure and its development.  The results showed that the characteristics of flower morphology are in accordance with the existing description in literatures. Characteristics of pollinia are specific characters of morphological aspect of flower. Data of anatomy of flower and its parts development are the new ones which confirm the position of C.racemosum as a member of the tribe Marsdenieae. The data of anatomy also show new information of the ontogeny of the important parts of flower: pollinia formation, pollinia corpusculum, anther wall, anther sac, stigma, stamen, staminal tube, stigmatic chamber, and structure of ovary in Asclepiadoideae
Substitution and Haplotype Diversity Analysis on The Partial Sequence of The Mitochondrial DNA Cyt b of Indonesian Swamp Buffalo (Bubalus bubalis) Akhmad Sukri; Mohamad Amin; Aris Winaya; Abdul Gofur
Biology, Medicine, & Natural Product Chemistry Vol 3, No 2 (2014)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1514.606 KB) | DOI: 10.14421/biomedich.2014.32.59-63

Abstract

This research aims to investigate the substitution pattern of nucleotide base and haplotype diversity of Indonesian swamp buffalo (Bubalus bubalis) based on the mitochondrial DNA cyt b partial gene sequence. 17 samples were chosen from 7 different regions with each uniquely represents Indonesian biogeography which comprise Aceh, Riau, Madiun, Blitar, Lombok, South Borneo and Tana Toraja. The result of cyt b gene sequence alignment showed the presence of transition and transversion substitutions and the absence of insertion and deletion. The amount of transitions was found to be higher than that of transversions and the amount of substitution in pyrimidine was also higher than that in purine. The highest amount of transitions happened in base TàC which is a silent substitution. The result of median joining network analysis showed that Indonesian haplotype Bubalus bubalis could be classified into 16 haplotypes which form different haplogroups unique to their geographical region. The result of median joining network analysis also indicated that the genetic relationships of swamp buffaloes (Bubalus bubalis) in Indonesia are highly influenced by their geographical locations.