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IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR Nugrahaningsih, Nugrahaningsih; Yuniastuti, Ari
Sainteknol : Jurnal Sains dan Teknologi Vol 12, No 2 (2014): December 2014
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v12i2.5415

Apoptosis adalah kematian sel terprogram (programed cell death) yang bertujuan untuk mempertahankan kestabilan populai sel. Kegagalan pengaturan apoptosis dapat menyebabkan sel membelah tanpa terkendali, yang disebut sebagai kanker. Peningkatan apoptosis merupakan suatu upaya yang dikembangkan sebagai terapi kanker. Pemeriksaan apoptosis dengan metode TUNEL(Terminal deoxyribonucleotidyl transferase dUTP nick end labeling) dapat memberikan gambaran proses apoptosis pada tingkat sel tunggal sehingga lebih spesifik dan memiliki akurasi tinggi. Sambiloto merupakan salah satu tanaman obat tradisional yang digunakan sebagai anti kanker. Beberapa penelitian yang dilakukan secara in vitro menunjukkan pengaruh andrografolid yang diisolasi dari sambiloto terhadap apoptosis sel kanker pada kultur. Hasil tersebut masih memerlukan penelitian lebih lanjut, terutama pengaruhnya bila diberikan secara oral. Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.

PENGEMBANGAN MULTIMEDIA SMEDIG MATERI SISTEM REPRODUKSI MANUSIA TINGKAT SMA Chasanah, Siti Nur; Mustikaningtyas, Dewi; Nugrahaningsih, -
Journal of Biology Education Vol 4 No 3 (2015)
Publisher : Journal of Biology Education

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Materi sistem reproduksi manusia memiliki karakteristik dengan banyaknya proses yang panjang. Observasi dan wawancara yang dilakukan di SMA N 1 Kendal, SMA N 2 Kendal dan SMA Pondok Modern Selamat menunjukkan bahwa buku yang menjadi sumber belajar belum dapat menjelaskan proses panjang tersebut. Pembelajaran web-browsing yang dilakukan guru beresiko terhadap penyalahgunaan akses yang mengarah pada pornografi. Sekolah memiliki sarana dan prasarana yang memadai tapi belum termanfaatkan secara optimal. Penelitian ini bertujuan untuk mengembangkan multimedia yang layak dan efektif pada materi sistem reproduksi. Multimedia yang dikembangkan adalah Science Multimedia Inquiry and Game (SMEDIG). Multimedia SMEDIG mengadaptasi sintaks pembelajaran guided inquiry dan dikombinasikan dengan education game. Metode yang digunakan dalam penelitian ini adalah metode Penelitian dan Pengembangan (Research and Development). Uji coba produk dilaksanakan di SMA Negeri 2 Kendal. Penelitian ini menghasilkan produk multimedia SMEDIG yang layak dan efektif untuk diterapkan dalam pembelajaran. Hal ini dibuktikan oleh tercapainya indikator kelayakan dan keefektifan multimedia SMEDIG. Persentase aktivitas klasikal peserta didik mencapai 83,75% dengan kriteria sangat aktif dan rata-rata ketuntasan klasikal hasil belajar peserta didik mencapai 95,6%, sehingga dapat disimpulkan bahwa multimedia SMEDIG yang dikembangkan layak dan efektif diterapkan dalam pembelajaranÂ Human reproductive system has many characteristics with length process. Observations and interviews at SMA N 1 Kendal, SMAN 2 Kendal and SMA Pondok Modern Selamat showedÂ that books became learning sources have not been able to illustrate such a long process. Web-browsing learning system was risky of unauthorized access that lead to pornography. The school has adequate facilities and infrastructure but has not been utilized optimally. This study aimed to develop a feasible and effective multimedia of human reproduktive systems. Multimedia that is developed is a multimedia was Science Multimedia Inquiry and Game (SMEDIG).Â SMEDIG adapts the syntax of guided inquiry learning and combined with education games. The product was tested at SMAN 2 Kendal. This research used Research and Development method. This study provides a product of learning media in the form of multimedia SMEDIG which is feasible and effective to be applied in learning process. This was proven by the achievement indicators of the feasibility and the effectiveness of multimedia SMEDIG. The percentage of learnersâ classical activity reached 83.75% with criteria very active and the average of classical completeness learning outcomes of students reached 95.6%, it be concluded that multimedia SMEDIG which develop feasible and effective to be applied in learning process. Â

PROGRAM INTEGRATED EDUSPORTAINMENT DI UNIVERSITAS NEGERI MEDAN (UNIMED) â€“ LITERASI BUDAYA BATAK DALAM AKTIVITAS OUTBOUND PADA PESERTA DIDIK USIA DINI Zainal, Andri; Simatupang, Nurhayati; Nugrahaningsih, R. H. D.; Sagala, Gaï¬€ar Hafz
Jurnal Abdimas Vol 21, No 1 (2017): Juni 2017
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LP2M), Universitas Negeri Semarang

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Program Integrated EduSportainment di UNIMED merupakan salah satu upaya UNIMED dalam memfasilitasi salah satu program unggulan pemerintah dalam penyediaan taman pintar atau yang disebut dengan science and technology park (STP) di Indonesia dengan keunggulan utama pada utilisasi seluruh sarana dan prasarana yang ada di UNIMED. Pencanangan tema spesifk tersebut menjadi keunggulan daya saing tersendiri bagi keberadaan kegiatan Integrated EduSportainment di UNIMED yang merupakan aktualisasi dari Program Iptek Bagi Kreativitas dan Inovasi Kampus (IbKIK) atau yang sekarang dikenal sebagai Program Pengembangan Unit Produk Intelektual Kampus (PPUPIK) dimana tidak hanya ditujukan bagi penguatan dan pelestarian budaya suku Batak namun juga menjadi salah satu kontribusi UNIMED sebagai salah satu pengemban amanat pendidikan nasional dalam memfasilitasi program pemerintah pusat untuk menjadikan Sumatera Utara sebagai salah satu destinasi wisata unggulan melalui Danau Toba sebagai â€œThe Monaco of Asiaâ€. Secara spesifk untuk jangka panjang, program IbKIK Integrated EduSportainment ini juga dicanangkan menjadi laboratorium terpadu bagi seluruh civitas akademika khususnya mahasiwa/i UNIMED dalam mempraktekkan ilmu kependidikannya melalui partisipasi mereka didalam operasional paket-paket wisata edukasi yang menyasar para peserta didik mulai dari tingkat taman kanak-kanak sampai ke tingkat sekolah umum/kejuruan sebagai target pasar produk dari IbKIK Integrated EduSportainment. Tim Pelaksana pada tanggal 22 Juli 2017 telah berhasil dan sukses menyelenggarakan kegiatan soft-launching kegiatan IbKIK Integrated EduSporTainment yang melibatkan 50 siswa/i dari Kelas VI SD Muhammadiyah Terpadu 36 Medan Denai. Kegiatan yang dimaksud berfokus pada 3 (tiga) aktivitas utama yakni Permainan Outbouind Cerdas yang mencakup permainan labirin, bola ornamen, ketapel tembak, puzzle dan bola estafet; workshop kuliner Batak (Ombus-Ombus dan Itak-Itak Pohul) dan; workshop tarian tradisional Batak Toba

Study of Anthocyanin Pigment in Three Different Sepal Colour of Hortensia (Hydrangea macrophylla) Hariri, Muhammad Rifqi; Nugrahaningsih, Nugrahaningsih; Balqis, Balqis
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Anthocyanin is a natural pigment that express orange, red, blue, and purple color. It can be accumulated in leaves, thorn, tuber, fruit, flower, and seed. Hortensia (Hydrangea macrophylla) which is well-known as âPanca Warnaâ, is a kind of plant that easy to thrive in different climates, can express different sepal colour as the effect of environmental factor such as soil pH. The aim of this study is to determine the level of anthocyanin pigment in three different sepal colour of Hortensia. This research is a descriptive quantitative which used randomized block design with nine replications. The object of this research is three different sepal colour of Hydrangea which obtained from Flower Gardens in Sidomulyo florist. Data obtained through the differential pH spectroscopy method and was analyzed using one way ANOVA. The result reveals that there is no significant differences of anthocyanin level among the samples.Keywords: anthocyanin pigment, sepal colour, Hortensia (Hydrangea macrophylla)

Study of Anthocyanin Pigment in Three Different Sepal Colour of Hortensia (Hydrangea macrophylla) Hariri, Muhammad Rifqi; Nugrahaningsih, Nugrahaningsih; Balqis, Balqis
Proceeding International Conference on Global Resource Conservation Vol 4, No 1: Proceeding of 4th ICGRC 2013
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Anthocyanin is a natural pigment that express orange, red, blue, and purple color. It can be accumulated in leaves, thorn, tuber, fruit, flower, and seed. Hortensia (Hydrangea macrophylla) which is well-known as âPanca Warnaâ, is a kind of plant that easy to thrive in different climates, can express different sepal colour as the effect of environmental factor such as soil pH. The aim of this study is to determine the level of anthocyanin pigment in three different sepal colour of Hortensia. This research is a descriptive quantitative which used randomized block design with nine replications. The object of this research is three different sepal colour of Hydrangea which obtained from Flower Gardens in Sidomulyo florist. Data obtained through the differential pH spectroscopy method and was analyzed using one way ANOVA. The result reveals that there is no significant differences of anthocyanin level among the samples.Keywords: anthocyanin pigment, sepal colour, Hortensia (Hydrangea macrophylla)

Developing of instructional media-based animation video on enzyme and metabolism material in senior high school Yusuf, Muhammad Mustofa; Amin, Mohamad; Nugrahaningsih, Nugrahaningsih
Jurnal Pendidikan Biologi Indonesia Vol 3, No 3 (2017): NOVEMBER
Publisher : University of Muhammadiyah Malang

The research aimed to product a learning material related to animation video on enzyme and metabolism material for high school student which is validated by media and material experts, educational practition and student legibility. Research and development model is ADDIE with quantitative-qualitative data analyzing methode. Data collection was obtained from validation results by media and material experts, educational partition and student legibility. The validation results were scores and suggestion. The percentage of product from expert media validation (100%), expert material validation (89,58%), educational practition (84,61%), and student legibility (81,91%) showed valid of the criteria and feasible to use after revision.

Efektivitas Seng (Zn) sebagai Imunostimulan dalam Produksi Reactive Oxygen Intermediate pada Mencit Balb/C yang Diinfeksi Salmonella typhimurium Yuniastuti, Ari; H, Nugrahaningsih W; Zunikhah, Zunikhah
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1151

This study aims to prove the effectiveness of zinc as a micronutrient to increaseimmunostimulatory Reactive Oxygen Intermediate (ROI) production in balb/c miceinfected by Salmonella typhimurium. Research was designed using randomized thePost Test Only Control Group Design. The sample of 24 male mice balb/c are groupedinto four groups, i.e. one control group and three treatment groups (treatment dose of 78ppm/day; 169 ppm/day and 260 ppm/day) intraperitoneally for 14 days with each groupconsisting of six rats. The independent variable is the provision of zinc with various doses,dependent variable is the ROI production of macrophages, which are checked by usingNitroblue Tetrazolium (NBT) Reduction Assay, and control variables are body weight,gender and age. All mice infected with Salmonella typhimurium 105 intraperitoneally onday 8th. At the end of treatment mice were killed and was examined for levels of ROI.Inspection results were analyzed by Kruskal Wallis test. Result showed that doses ofzinc of 78 ppm/day have immunostimulatory effective as the highest ROI productionof mice Balb/c infected with Salmonella typhimurium.Keywords: immunostimulant, Reactive Oxygen Intermediate, zinc (Zn)

ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI PETERNAKAN TRADISIONAL KECAMATAN GUNUNGPATI SEMARANG Wibowo, Angga Ari; Susanti, R.; Ulum, Farikhul; W.H., Nugrahaningsih
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2270

Avian Influenza (AI) atau yang lebih dikenal dengan flu burung disebabkan oleh virus influenza yang bermutasi menjadi patogen. Penelitian tentang isolasi dan identifikasi virus AI subtipe H5N1 perlu dilakukan untuk mengetahui keberadaan virus tersebut khususnya di kecamatan Gunungpati. Desain penelitian adalah eks ploratif dengan pengumpulan sampel usap kloaka secara acak di lima kelurahan di kecamatan Gunungpati. Sampel usap kloaka ditumbuhkan pada telur ayam berembrio SPF, kemudian diisolasi RNA-nya dilanjutkan dengan identifikasi subtipe virus AI menggunakan Reverse Transcriptase-Polymerase Chain Reaction (RTPCR) dengan primer pendeteksi gen H5 dan N1. Hasil positif apabila visualisasi hasil elektroforesis dari produk PCR menunjukkan pita-pita spesifik panjang 219 bp untuk H5 dan 131 bp untuk gen N1-nya. Limapuluh sampel usap kloaka yang diisolasi dari lima kelurahan di Gunungpati, delapan isolat positif VAI dan enam diantaranya positif H5N1 dengan angka prevalensi 12%. Isolat positif berasal dari 2 spesies itik (16,67%), 2 dari entok (11,76%) dan 2 dari angsa (18,18%). Dari lima kelurahan yang diambil sampelnya, tiga kelurahan ditemukan positif virus H5N1 masing-masing kelurahan Sekaran (6,67%), Kalisegoro (16,67%) dan Pakintelan (15,78%). Unggas-unggas air di peternakan unggas tradisional berpotensi sebagai penularan virus AI, khususnya subtipe H5N1.Avian Influenza (AI) or better known as bird flu is caused by influenza viruses that mutate into a pathogen. Research on the isolation and the identification of H5N1 subtype needed to be carried out to determine the presence of the virus, particularly in the subdistrict of Gunungpati. The study design was explorative by collecting cloacal swab samples randomly from five villages in Gunungpati. The cloacal swab samples were cultured in embryonated SPF chicken eggs, then the RNA was isolated and followed by the identification of AI virus subtype using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) with H5 and N1 gene detecting primers. A positive result is obtained if the visualization of the electrophoresis of PCR products showed bands with specific length of 219 bp for H5 gene and 131 bp for N1 gene. Fifty cloacal swab samples were isolated from five villages in Gunungpati, and eight of them were positive isolates and six of them were H5N1 positive with the prevalence rate of 12%. The positive isolates were derived from two species of duck (16,67%), 2 from wild duck (11,76%) and 2 from geese (18,18%). Of the five sampled villages, three villages were found to be H5N1 positive, i.e. Sekaran village (6,67%), Kalisegoro village (16,67%) and Pakintelan village (15,78%). Water birds in traditional poultry farming were considered potential as the transmitters of AI virus, particularly of H5N1 subtype.

Analysis of Implementation Plan of Science Learning and the Factors of ITS Implementation in MTs and MA Al Khoiriyyah Semarang Sabdhosih, Has; Isnaeni, Wiwi; Nugrahaningsih, Nugrahaningsih
Journal of Innovative Science Education Vol 7 No 2 (2018)
Publisher : Postgraduate, Universitas Negeri Semarang, Indonesia

The learning process requires a plan, so that what is done can run and produce something as expected. With such a plan, the process to be carried out over a long period of time has a clear direction, predictable results, predictable resources required, and can be used to determine the requirements of students in following the learning process in the school. The purpose of this study is to analyze the conformity and implementation of RPP, as well as supporting factors and inhibiting the implementation of science learning in MTs and MA Al Khoiriyyah Semarang. The method used in this research is qualitative research with case study method. This research takes place in Madrasah Tsanawiyah (MTs) and Madrasah Aliyah Al Khoiriyyah Semarang. Sources of data in this study are teachers of science lessons in MTs Al Khoiriyyah and physics, chemistry, biology teacher at MA Al Khoiriyyah. Data collection techniques used consisted of observation, interview, documentation and student response questionnaire. The results showed that the quality of RPP in MTs and MA Alkhoiriyyah was in accordance with Permendikbud number 22 of 2016 with very good criteria. The implementation of science learning in MTs and MA Alkhoiriyyah has been in accordance with Permendikbud number 22 of 2016 with good category. Factors supporting the implementation of RPP in learning is the source of learning and supporting facilities such as libraries, laptops, LCDs, and the internet. While the inhibiting factors are time constraints, students are less active, and the teacher's understanding is still lacking in combining the methods, models, and learning strategies with a scientific approach. Implementation of science learning activities in MTs and MA Al Khoiriyyah received a good response from learners.

Development of Alternative Plant Tissue Culture Module to Optimize Science Process Skills and Curiosity in Modern Biotechnology Learning in High School Nihayah, Durrotun; Rahayu, Enni Suwarsi; Nugrahaningsih, Nugrahaningsih
Journal of Innovative Science Education Vol 8 No 1 (2019): Article In Press
Publisher : Postgraduate, Universitas Negeri Semarang, Indonesia

The ideal modern biotechnology is done by practicum. Practicum of plant tissue culture, especially in vitro germination, is intended to train students to perform aseptic activities according to tissue culture characteristics, but not to obtain substantial culture results. This study aims at test the effectiveness of alternative plant tissue culture techniques as a development of alternative plant tissue culture module to optimize science process skills and curiosity learning in high school. The research method uses the ADDIE instructional model. The data taken are in the form of the effectiveness of tissue culture techniques, module validity by media and material experts, module effectiveness seen from the value of science process skills and curiosity, the practicality of the module seen from the response of users namely teachers and students. The results showed that alternative plant tissue culture techniques were effectively used as practical material on the topic of tissue culture in high school to practice science process skills; the modules developed were considered very valid, effective, and practical; the developed modules had characteristics compiled based on simple research on culture network, equipped with images to clarify the practical process, equipped with observation tables as testing data that can guide students to find the concepts taught Based on these results the module is recommended, the use of modules for practicum must manage the time so that everything is in well condition of learning.

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