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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Microbiology Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
MEITY SURADJI SINAGA
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology

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 1 
Rapid Detection of Bacterial Pustule Disease on Soybean Employing PCR Technique with Specific Primers ANDI KHAERUNI; ANTONIUS SUWANTO; BUDI TJAHJONO; MEITY SURADJI SINAGA
HAYATI Journal of Biosciences Vol. 14 No. 2 (2007): June 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.581 KB) | DOI: 10.4308/hjb.14.2.76

Abstract

A rapid polymerase chain reaction (PCR)-based procedure was developed for detection of Xanthomonas axonopodis pv. glycines, the causal agent of bacterial pustule disease on soybean. A set of primers was designed from partial sequence of the pathogenicity gene of X. axonopodis pv. glycines strain YR32. Specific PCR product of 490 base pairs was produced from strains of X. axonopodis pv. glycines originally from Indonesia as well as from Taiwan. No other pathovars and bacterial species among those tested showed amplification product under optimized PCR conditions. Shaking infected soybean leaves in phosphate buffer saline during six hours was proved to be an essential in order to increase cell number of the bacterial. The procedure was applicable and reliable for detecting of pathogens in infected plant materials. The procedure was proved to be more effective than that of conventional detection and could be of great help for monitoring of pustule bacterial disease in the soybean fields. Key words: Xanthomonas axonopodis pv. glycines, bacterial pustule disease, rapid detection, PCR, specific primer
Isolation and Identification of Mycorrhizosphere Bacteria and Their Antagonistic Effects Towards Ganoderma boninense in vitro YENNI BAKHTIAR; SUDIRMAN YAHYA; WAHONO SUMARYONO; MEITY SURADJI SINAGA; SRI WILARSO BUDI; TEUKU TAJUDDIN
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8578.755 KB) | DOI: 10.5454/mi.4.2.9

Abstract

Basal stem rot caused by Ganoderma boninense is the most serious disease of oil palm (Elaeis guineensis) in Indonesia and it has caused major loss in palm oil production. Mycorrhizosphere bacteria offer possible advantages as biocontrol agents as they live and proliferate together with arbuscular mycorrhizal fungi, which have an ability to increase plant resistance against pathogens. A study was conducted to isolate mycorrhizosphere bacteria from spores of arbuscular mycorrhizal fungi and test their antagonistic effects against G. boninense in vitro. All bacterial isolates were identified based on 16S rDNA analysis and it revealed that eleven out of twenty mycorrhizosphere bacteria isolated were related to Bacillus with similarity ranging from 97 to 100%, whereas other isolates were identified as Pseudomonas, Streptomyces, Kocuria, Enterobacter, Brevundimonas, and Alcaligenes with similarities ranging from 96 to 100%. Fourteen out of twenty mycorrhizosphere bacteria showed a varying degree of inhibition towards the growth of G. boninense in vitro. Of these, isolate B10 (closely related to Bacillus subtilis ZJ06) showed the highest inhibitory effect followed by B17 (closely related to Bacillus subtilis N43). Therefore, these bacteria have a potential to be used as biocontrol agents to control basal stem rot disease caused by G. boninense in oil palm.
Diagnosis of Corm Rot Disease of Taro: Case Study at Maybrat - West Papua Paulus Woyen; Meity Suradji Sinaga; Efi Toding Tondok; Bonny Poernomo Wahyu Soekarno
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.177-184

Abstract

Diagnosis of Corm Rot Disease of Taro: Case Study at Maybrat - West Papua Taro (Colocasia esculenta L. Schott) is a herbaceous plant that has been used as food crop in some countries. It is believed that taro has originated from Southeast Asia (Lebot et al. 2010). In Maybrat, West Papua taro was used as a primary important staple food crop for ages by local tribes, and it is called Awiah or Wiah in this area. Until 2013, there is no proper pests and diseases problem reported in this area, but, in the middle of 2013 the local farmers reported an epidemic of corm rot disease on their taro crops. The aim of this study was to identify the causal agent of corm rot disease of taro in Maybrat. For disease diagnosis, pathogen isolation was carried out on samples of rotted corm collected from local farmers in Maybrat and followed by Koch Postulate. The assay was carried out in vivo and in planta to identify the corm rot pathogen. Out of 38 fungi isolated from the diseased corms, 50% of these were pathogenic. Three isolates called Y1, YP1, and S4 were able to cause corm rotting in vivo and root damage in planta. In planta assay showed that, combination of these isolates could cause severely root damage. Morphologically, isolate YP1 and S4 were identified as Fusarium oxysporum, while isolate Y1 was comfirmed genetically as F. solani. This is the first report of taro corm rot disease from Maybrat, West Papua.
Effectiveness of Endophytic Bacteria and Arbuscular Mycorrhizal Fungi in Suppressing Ralstonia solanacearum in Eucalyptus pellita Plants Yuliana Susanti; Giyanto Giyanto; Meity Suradji Sinaga; Kikin Hamzah Mutaqin; Budi Tjahjono
Jurnal Fitopatologi Indonesia Vol 16 No 4 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.4.166-176

Abstract

Penyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu penyakit penting pada tanaman eukaliptus (Eucalyptus pellita) di Indonesia. Salah satu pendekatan teknik pengendalian adalah melalui pemanfaatan bakteri endofit dan fungi mikoriza arbuskula (FMA). Penelitian ini ditujukan untuk mengevaluasi keefektifan bakteri endofit dan FMA dalam menekan perkembangan penyakit layu bakteri. Terdapat sepuluh kombinasi bakteri endofit dan FMA yang di-ujikan pada bibit eukaliptus berumur satu bulan. Inokulasi R. solanacearum secara buatan dilakukan tiga bulan setelah introduksi bakteri endofit dan FMA. Percobaan dilakukan di ruang growth chamber. Peubah pengamatan meliputi periode inkubasi, insidensi penyakit, laju penyakit, agresivitas kolonisasi bakteri dengan mengamati cairan bakteri (ooze) R. solanacearum pada bibit eukaliptus, pengukuran aktivitas phenylalanine ammonia lyase (PAL), dan total fenol. Hasil penelitian menunjukkan bahwa perlakuan B5F1 (Paenibacillus polymyxa dan Glomus mosseae) memiliki kemampuan penekanan tertinggi terhadap perkembangan penyakit layu bakteri pada bibit eukaliptus, yaitu sebesar 100%. Perlakuan B5F1 menunjukkan periode inkubasi R. solanacearum lebih lama, persentase insidensi penyakit paling rendah, laju infeksi penyakit rendah, dan penghambatan agresivitas kolonisasi R. solanacearum pada eukaliptus. Sementara perlakuan B4F1 (Serratia marcescens dan G. mosseae) mampu meningkatkan aktivitas PAL dan total fenol tertinggi dibandingkan perlakuan lainnya. Berdasarkan hasil penelitian ini, perlakuan B5F1 dan B4F1 berpotensi mengendalikan penyakit layu bakteri pada tanaman eukaliptus.
Hemolysis and hypersensitive tests ease culture collection management of antagonistic bacteria Amaria, Widi; Meity Suradji Sinaga; Kikin Hamzah Mutaqin; Supriadi; Widodo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22324-30

Abstract

A biosafety assessment is a crucial first step in the management of microbial culture collection to screen and determine unexpected potential plant and human pathogenic bacteria. It is common to collect and store as many fresh culture collections from natural resources before being further evaluated for antagonist bacteria. As a result, a bulk of isolates must be preserved which required more effort and budget. Safety evaluations based on the hemolysis and hypersensitive reactions offer simple tests to ease culture collection management of antagonist bacteria. The study aimed to evaluate the safety of bacterial culture collection for their hemolysis and hypersensitive reactions. Ninety-five isolates of rhizosphere and endophytic bacterial isolates from the culture collections of the Department of Plant Protection-IPB University, Indonesian Industrial and Beverages Crops Research Institute, and Indonesian Spice and Medicinal Crops Research Institute, were evaluated their safety using the hemolysis and hypersensitive tests. The hemolysis test was conducted using blood agar media, from which isolates with a negative (?) reaction were then tested for the hypersensitivity reaction on tobacco leaves. Bacterial isolates passed from both hemolysis and hypersensitivity tests were then preserved by the lyophilization method for long-term storage of culture collection. Based on the hemolysis test, 68 out of 95 bacterial isolates (71.57%) were found to be positive (? or ?) reactions. The hypersensitive test showed that 22 of 27 negative hemolysis isolates did not trigger hypersensitivity reactions in tobacco leaves, therefore, they were preserved by lyophilization. The study indicated that a high number of bacterial isolates in the present collection, 68 positive hemolysis, and 5 hypersensitive, need to be re-evaluated due to their safety concerns. The present study highlights the importance of biosafety tests performed in an early stage before the to permanent collection of antagonist isolates.
Co-Authors Andi Khaeruni Antonius Suwanto Bonny Poernomo Wahyu Soekarno Budi Tjahjono Efi Toding Tondok Kikin H Mutaqin Laksono Trisnantoro Paulus Woyen Sri Wilarso Budi Sudirman Yahya Supriadi Teuku Tajuddin Wahono Sumaryono Widi Amaria Widodo YENNI BAKHTIAR Yuliana Susanti