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All Journal Prosiding Seminar Biologi JOURNAL ONLINE OF PHYSICS JURNAL AGRILAND BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Faktor Exacta Dinamisia: Jurnal Pengabdian Kepada Masyarakat Bioedukasi: Jurnal Pendidikan Biologi Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Jurnal Biosense Sciening : Science Learning Journal Bioscientist : Jurnal Ilmiah Biologi EduBiologia: Biological Science and Education Journal Berkala Penelitian Hayati Bioscientiae, Jurnal ilmu ilmu Biologi Jurnal Pengabdian Masyarakat Biologi dan Sains Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)

Fitri Damayanti

Universitas Indraprasta PGRI

Author-ID : 2638565

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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INDUKSI POLIPLOIDI DENGAN KOLKISIN PADA HIBRID F1 HASIL PERSILANGAN ANTAR SPECIES PADA TANAMAN PANILI ASAL CIAMIS Damayanti, Fitri; Mariska, Ika
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3455

Steem root disease caused by Fusarium oxysporum Schl. f. sp. Vanillae (Tucker) Gordon represent one internal issues of vanilla development (Vanilla planifolia Andrews). To obtain resistance clone to the disease, it can exploit resource of wild vanilla (V. albida B. L Syn) through crosses. Hybrids which were interspecific crossed generally were sterile. To overcome sterility problem of the hybrids, chromosome doubling was made by colchicine application. Explant used globular structure of proembryo from F1 seed result from a cross between wild vanilla of Ciamis as female parents and cultivated vanilla clone of Ciamis as male parent. Concentration level colchicine used were 0.00%, 0.05%, 0.10%, 0.20% and 0.25% with period of treatment of 3 and 6 days. After colchicine treatment embryo cultures were subcultured into new mwdium that was basal media Murashige-Skoog enriched with 2.5 mg/l BAP. Result of the experiment showed that colchicine treatment,globular structure were F1 embryo tending to inhibit early regeneration. The cultures showed variabilities from treatment of colchicin 0.20% during of 6 day and 0.25% for 3 days. Phenotypic performance of the chromosome doubled hybrids showing great variation in color and vigor of the culture. Tetraploid plant(2n=4x=64) was obtained from the colchicine treatment of 0.25% for 6 days. Chromosome addtion was followed by improvement of cell dimensions and organ magnification.

PENGARUH RADIASI SINAR GAMMA DAN ASAM FUSARAT UNTUK MENINGKATKAN KETAHANAN ABAKA (MUSA TEXTILIS NEE) TERHADAP FASARIURN OXYSPORUM Damayanti, Fitri; Suharsono, Suharsono; Mariska, Ika
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3285

ABSTRACTEffect of gamma radiation and fusaric acid for resistance to wild Fusarium disease onabaca plant (Mum textillis Nee). The problem in abaca production is wilt disease infectioncaused by Fusarium oxysporzim. The resistant variety against the pathogen has not beenavailable yet. The disease resistance character of the species might be improved throughsomaclonal variation and in vitro selection. Different pure toxin of fusaric acid concentration(0, 15,30,45,60, and 75 mg/l) was used as component selection to get new hope numbers ofresistant abaca to wilt Fusarium disease through in vitro selection. Concentration of 45 mgllfusaric acid is lethal for abaca, so we used this concentration as dose of selection to selectshoots from irradiated calli. Gamma irradiation was used as mutagent to increase somaclonalvariation on abaca. Six levels of gamma-ray radiation (0, 0.5, 1, 2, and 3 Krad) were appliedto embriogenic calli. Increasing dose of radiation decreased the viability of calli. LDso wasfound between 1-1.5 Krad of radiation dose. In vitro selection was carried out in two stages.The concentration of selection of hsaric acid in stage I1 was increased one level to theconcentration in stage 1. Stage I selection of shoots from irradiated calli on mediumcontaining pure toxin 45 mg/l fusaric acid, showed that the survival capacity decreasing withthe increasing doses of gamma irradiation. In stage 11, shoots from irradiated calli (at 0.5 and1 Krad) could survive on medium containing 60 mg/l fusaric acid. In medium selectioncontaining 50% filtrate F. oxysporum, fusaric acid resistant shoots were also filtrate resistant.There was a correlation between in vitro fusaric acid and filtrate of F. oxysporum resistantplant and conidia suspension of F. oxysporum resistkt plant in the greenhouse.Keywords: Gamma radiation, in vitro selection, fusaric acid, Musa textilis, Fusariumoxysporum

Bercocok Tanam Dengan Sistem Hidroponik Berbasis Ramah Lingkungan Melalui Pemanfaatan Sampah Botol Plastik Fitri Damayanti; Titin Supriyatin
Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) Vol 4, No 1 (2020): Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM Universitas Respati Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52643/pamas.v4i1.724

ABSTRAKSampah plastik termasuk botol plastik adalah jenis sampah yang sangat sulit terurai sehingga menimbulkan masalah kebersihan dan kesehatan lingkungan. Secara umum masyarakat belum memiliki kemampuan dalam mengolah sampah botol plastik sehingga terjadi penumpukan. Sampah botol plastik tersebut belum termanfaatkan menjadi sesuatu yang berguna. Permasalahan lain yang dihadapi mitra adalah terbatasnya lahan dan waktu yang dimiliki mitra untuk bercocok tanam. Solusi yang ditawarkan adalah penerapan teknik bercocok tanam dengan sistem hidroponik berbasis ramah lingkungan dengan memanfaatkan sampah botol plastik. Kegiatan dilakukan dengan mentransfer pengetahuan kepada mitra melalui pelatihan pembuatan perangkat sistem hidroponik sederhana menggunakan botol plastik sebagai media tanam sayuran. Kegiatan pengabdian kepada masyarakat dilakukan dengan mitra PKK Karang Anyar, Kecamatan Sawah Besar, Jakarta Pusat. Kegiatan ini bertujuan memperkenalkan cara bercocok tanam dengan sistem hidroponik berbasis ramah lingkungan melalui pemanfaatan daur ulang botol plastik. Kegiatan ini merupakan salah satu terobosan dalam mengurangi masalah plastik dengan menciptakan lingkungan yang bersih, hijau, dan asri. Setelah pelatihan terjadi peningkatan pengetahuan mitra mengenai pemanfaatan sampah botol plastik dan terbukti juga berhasil meningkatkan minat peserta untuk memanfaatkan botol plastik bekas untuk bercocok tanam di rumah. Mitra memberikan respon yang sangat positif terhadap kegiatan yang telah dilakukan dan menilai kegiatan ini sangat bermanfaat. Selain itu, kegiatan ini dapat mendorong kemandirian ekonomi mitra dengan mengurangi biaya belanja rumah tangga sehari-hari terutama sayuran.Kata kunci: botol plastik, daur ulang, hidroponik ABSTRACT Plastic waste as well as plastic bottles is a type of waste that are very difficult to decompose. That has caused environmental and hygiene problems. In general, the community does not yet have the ability to process plastic bottle waste that cause buildup. Plastic bottle waste has not been much used as something useful. Others problems that has againt partner are limited land and time to grow crops. The solution offered was the application of agricultural techniques using environmentally friendly hydroponic systems based on plastic bottle waste. This activity was carried out by transferring knowledge to partner through training in making a simple hydroponic system using plastic bottles as a medium for growing vegetables. Community service activities were carried out with PKK Karang Anyar, Sawah Besar Regency, Jakarta as a partner. This activity aimed to introduce how to plant with an environmentally friendly hydroponic system based on the use of recycled plastic bottles. This activity is one of the breakthroughs in reducing plastic problems by creating a clean, green, and beautiful environment. After the training there was an increase in partner's knowledge about the use of plastic bottle holders and it was proven that it also succeeded in increasing participants' interest in using plastic bottles for farming at home. Partner gave very positive response to the activities that have been carried out and feel this activity was very beneficial. In addition, this activity could encourage the economic independence of partners by reducing the cost of daily household expenses, especially vegetables.Keywords: plastic bottle, recycle, hydroponic

Survei dan inventarisasi keanekaragaman jenis Nepenthes spp. di Kalimantan Tengah Fitri Damayanti; Muhammad Mansur; Ika Roostika
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15800

Kegiatan eksplorasi keanekaragaman tanaman Nepenthes dilakukan di Kalimantan Tengah dengan lokasi Hampangen, Pendahara, Hampalit, dan Honggang. Kegiatan ini meliputi: pemeriksaan spesimen herbarium di Herbarium Bogoriense, kunjungan ke pembibitan Nepenthes di Palangkaraya, komunikasi pribadi dengan para penghobi Nepenthes di Palangkaraya, dan studi literatur yang digunakan untuk mengetahui keragaman Nepenthes di lokasi penelitian. Hasil kegiatan eksplorasi dan koleksi di empat lokasi penelitian yang berada di Kabupaten Katingan maka dapat dilaporkan bahwa terdapat 12 jenis Nepenthes yang terbagi dalam lima jenis murni dan tujuh jenis hibrid alami, antara lain adalah: N. gracilis, N. mirabilis, N. rafflesiana, N. ampullaria, N. reinwardtiana, N. rafflesiana x N. ampullaria (N. xhookeriana), N. gracilis x N. mirabilis (N. xneglecta), N. ampullaria x N. gracilis (N. xtrichocarpa), N. reinwardtiana x N. mirabilis, N. reinwardtiana x N. gracilis, N. reinwardtiana x N. rafflesiana, dan N. rafflesiana x N. mirabilis. Akan tetapi tiga jenis Nepenthes yang menjadi koleksi utama, yaitu: N. hirsuta, N. hispida, dan N. stenophylla tidak terdapat pada keempat lokasi tersebut. Hal ini diduga karena telah terjadi kerusakan pada habitat aslinya yang berubah menjadi daerah pertambangan serta tanaman Nepenthes yang diperdagangkan langsung diambil dari alam tanpa adanya upaya peremajaan atau budidaya. Apabila ditambahkan dengan data survei sebelumnya yakni dari daerah Barito Ulu, Sebangau, Kalampangan, dan data informasi lainnya, maka Kalimantan Tengah memiliki 18 jenis Nepenthes yang terbagi ke dalam 10 jenis murni dan delapan hibrid alami. Hutan gambut dan hutan kerangas merupakan habitat Nepenthes di Kalimantan Tengah. Kondisi kedua habitat tersebut kini sudah sangat memprihatinkan yang diakibatkan oleh kebakaran hutan, perambahan, penambangan emas, dan perubahan fungsi kawasan menjadi perkebunan kelapa sawit.

Aplikasi pupuk organik cair air kelapa terhadap pertumbuhan tanaman jahe (Zingiber officinale Rosc.) Emilda Emilda; Pina Oktapiani; Fitri Damayanti
AGRILAND Jurnal Ilmu Pertanian Vol 8, No 3 (2020): Agriland: Jurnal Ilmu Pertanian
Publisher : Universitas Islam sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/agr.v8i3.3496

Bertambahnya permintaan jahe harus diiringi peningkatan produktifitas lahan budidaya. Kenyataannya kualitas lahan makin menurun karena rusaknya unsur hara akibat pemupukan kimia terus menerus. Apalagi sebagai tanaman obat tanaman jahe membutuhkan pupuk yang tepat untuk menjaga kualitas senyawa bioaktif yang dikandungnya. Tujuan penelitian adalah untuk mengetahui respon pertumbuhan tanaman jahe dengan pemberian pupuk organik cair berbahan air kelapa. Metode penelitian menggunakan Rancangan Acak Lengkap Non Faktorial 4 ulangan dengan konsentrasi pupuk organic cair air kelapa sebagai perlakuan. Hasil penelitian menunjukkan bahwa terjadi peningkatan pertumbuhan tanaman jahe selama penanaman 80 hari. Perlakuan 15 ml pupuk/tanaman memberikan hasil terbaik untuk tinggi tanaman, jumlah daun dan lebar daun tanaman jahe.

Regeneration and histological study of somatic embryogenesis of sugarcane (Saccharum officinarum L.) cultivar PS 864 Fitri Damayanti; Suharsono Suharsono; Aris Tjahjoleksono; Ika Mariska
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

The process affecting somatic embryo formation in sugarcane is very specific for each genotype, so the determination of the best somatic embryo regeneration medium in sugarcane clones is necessary. The objective of this study was to determine the concentration of Benzine Amino Purine (BAP) and Kinetin for somatic embryo maturation and to observe the maturation stage for somatic embryo of sugarcane cultivar PS 864. Maturation of the nodular callus was conducted by addition of Kinetin (0, 1, 3, and 5 mg/L) and BAP (0 and 5 mg/L) in solid and liquid medium. The medium was optimized using glutamine. The medium for somatic embryo germination used full and half strength Murashige and Skoog medium (MS) supplemented with 20 g/L sucrose and amino acid or growth regulator (BAP and NAA). Nodular callus well grew in solid medium, whereas callus become browning and not existence of somatic embryo maturation in liquid medium. The highest number of globular embryos (38 embryos) was produced from MS medium supplemented with 1 or 3 mg/L Kinetin combined with 5 mg/L BAP. The highest number of scutellum (21 embryos) and coleoptile (19 embryos) resulted from 3 mg/L Kinetin. The MS medium with full strength was added with 100 mg/L glutamine that was the best germination medium. This medium resulted the highest percentage of somatic embryo (73.29%) forming bipolar structure, and the largest number of leaves (4.58). Histological analysis showed that somatic embryos of sugarcane emerged from many cells through the budding process and also initiated from one cell.

Induksi Keragaman Genetik pada Tanaman Alocasia Menggunakan Mutagen Kimia Kolkisin Fitri Damayanti; Zakiah Fithah A’ini
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 1 (2021): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v9i1.3753

Alocasia green velvet (neon taro) is one of the ornamental plants that plant lovers hunt. The increasing standard of living and interest in beauty and uniqueness, the demand for this plant has increased. This provides an opportunity for ornamental plant farmers to develop the potential of Alocasia. Genetic diversity in Alocasia is very low because generally these plants are propagated such as vegetative. Therefore, it is necessary to increase genetic diversity so that new types of Alocasia are produced with more attractive leaf shapes, sizes, and coloration. Efforts that can be applied are mutation induction using a chemical mutagen, namely colchicine. The plant material was used that the tuber of the Alocasia plant. The study was conducted using a randomized block design with two factors, the colchicine concentration (0, 10, 15, 20, 25, and 30 mgL-1) and the immersion time (1 and 2 days). The 30 mgL-1 colchicine treatment gave better growth: producing the highest number of leaves, the longest root length, the highest number of shoots and the percentage of sprouting tubers that reached 100%. Colchicine treatment of 10 mgL-1 with immersion time of 1 day resulted in a different leaf shape. Colchicine treatments of 10, 15, 20, and 25 mgL-1 produced plants with younger leaf coloration than the control. As well as the 30 mgL-1 colchicine treatment produced vigorous plants with larger stem sizes.

VARIASI SOMAKLONAL TANAMAN KANTONG SEMAR (NEPENTHES MIRABILIS DAN N. GRACILIS) SECARA IN VITRO DENGAN MUTAGEN KIMIA KOLKISIN FITRI DAMAYANTI; IKA ROOSTIKA
Faktor Exacta Vol 8, No 3 (2015)
Publisher : LPPM

Nepenthes are potential to be commercialized because of the shape and color of their unique pitcher. Generally, people are interested in new and rare types which have unique color, shape, and size of pitcher. However, the increase of its demand has led to the decrease of natural populations due to overexploitation in the natural habitats in Indonesian forests without sufficient cultivation or replacement efforts. The genetic erosion has resulted in low level variation of this species. In vitro plant breeding can be carried out through somaclonal variation. The objective of this study was to obtain somaclonal variation method by using colchicin as a chemical mutagen. In vitro shoot of N. mirabilis and N. gracilis were used in this research as explant that planted in ¼ MS + colchicin at the level of 0, 0.05, 0.075, and 0,1%. The application of colchicin could produced cultures with high level of growth than control. The best result was obtained from colchicin at a level of 0.05% as a alternative in vitro to improve genetic variation. Key words: N. mirabilis, N. gracilis, somackonal variation, colchicin

KOLEKSI PLASMA NUTFAH PISANG SECARA EX VITRO DAN IN VITRO SERTA KAJIAN SITOLOGI DAN ANALISA KERAGAMAN ANTAR KARAKTER BERDASARKAN PENANDA FENOTIPE Fitri Damayanti; Ika Roostika
Faktor Exacta Vol 3, No 2 (2010): Faktor Exacta
Publisher : LPPM

Indonesia is the one of banana biodiversity center in the world. Genetic diversity of this plant is large. Thus, it is important to conserve these genetic resources to support banana breeding program. Collection of banana germplasm is more important due to over exploitation in the natural habitats in Indonesian forests without sufficient culture or replacement efforts. Studies on cytology and morphological characters of plants are required to avoid duplications of germplasm collections. This research would be explored 30 accession of Indonesian banana germplasms from East Borneo, Banjarmasin (South Borneo), field germplasms from Cibinong, Yogyakarta, Rembang, Purworejo, and Subang. There were 20 accession of germplasms collections that would succeed to collect in vitro. Chromosome analysis revealed different ploidies: diploid (2n=2x=22) and triploid (2n=3x=33). Related similarity in dendogram from ten accessions of banana (Rotan, Telunjuk, Emas, Hutan, Lampung, Api Merah, Api Hijau, Morosebo, Tanduk, and Barangan) based of vegetative and generative characters divided into four groups at 88.75% similarity; group I were accessions of Emas, Telunjuk, Rotan, and Hutan;accessions of Lampung and Morosebo were group II; group III was accession of Tanduk; and the last group were accessions of Api Merah, Api Hijau and Barangan. Result from in vitro experiment, induction of banana bud was depending of fenolic acid that results from specific variety, growth hormone concentration, and genome. Banana with A genome (diploid AA or triploid AAA) had the best growth because the result of fenolic acid was lower. Culture media with 5 ppm BAP was elevating in vitro bud of banana germplasm that have more number of A genome. The banana whose have more number of B genome was need higher concentration of BAP. Ambon Kuning whose have AAA genome had the higher multiplication of bud rather than Api Hijau and Api Merah whose have AAB genome or Kepok Kuning with ABB genome. Key words: genome, chromosome, Musa spp., ploidy, stomata

Kajian Morfologi, Sitologi, dan Struktur Anatomi Daun Nepenthes spp. asal Kalimantan Barat FITRI DAMAYANTI; IKA ROOSTIKA; MUHAMMAD MANSUR
Bioedukasi: Jurnal Pendidikan Biologi Vol 8, No 2 (2015): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v8i2.3862

The objective was to describe morphology, structure of anatomy, and cytology characters of the five species as well as to determine their differences each other. In the present study five Nepenthes from West Kalimantan were evaluated: N. veitchii, N. negleta, N. bicalcarata, N. clipeata, and N. hirsuta. Morfology analysis of five species Nepenthes showed different morphology in particular shape of pitcher that each species have a unique pitcher. Cytology analysis showed high variation between species. In general, it was demonstrated that N. bicalcarata tended to have bigger epidermal cells and stomata. N. bicalcarata had aktinocytik tipe of stomata and N. clipeata, N. veitchii, N. hirsuta, and N. negleta had anomocytik tipe of stomata. Stomata only had on the lower surface except N. negleta had stomata on the upper too. N. negleta had the higher of density and index stomata. The result of transversal showed variation of mesofil tissue (parenchim of palisade and spons). Epidermis cell was conducted in one layer with hipoepidermis cell more than one layer with the biggest size than epidermis cell. N. veitchii had more tricoma from the others species.

Co-Authors . SUHARSONO Acep Musliman Ardianti Saputri Aris Tjahjoleksono Aryanto, Saring Avini Nurazhimah Arfa Ayelir Sukma Yolla Dheani Amalia Firdaus Dini Siti Nurahmah Efri Gresinta Emilda Emilda Erik Perdana Putra Fitri Damayanti Giry Marhento Ika Mariska Ika Mariska Ika Roostika IKA ROOSTIKA Ika Roostika Linda, Linda Mashudi Alamsyah Muhammad Mansur MUHAMMAD MANSUR Pina Oktapiani Rifqi Pratama Rosa Dewi Pratiwi Sri Murni Soenarno Suharsono Suharsono Supriyatin, Titin Sutoro Sutoro Titin Supriyatin Tulus Widjajanto Usep Kasman Yulistiana Yulistiana Zakiah Fithah A’ini

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