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The Antioxidant Activities and Phenolic Content of Improved Soybean Seeds Varieties of Different Grain Sizes Muchdar Soedarjo; Suhartina Suhartina; Novita Nugrahaeni; Andy Wijanarko; Devi Anggraini Putri; Sri Fatmawati
IPTEK The Journal for Technology and Science Vol 31, No 1 (2020)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.454 KB) | DOI: 10.12962/j20882033.v31i1.5961

Abstract

Tempe and tofu are the main product of soybean commonly consumed as a daily food for the people of Indonesia. So far, soybean is well known to be a source of protein. Indeed, soybean contains secondary metabolites resulting in antioxidant activities. Antioxidants would nullify the negative effect of reactive oxygen and, as a result, could improve and maintain human health. Improvement of awareness of Indonesian people that soybean is good for human health promotion and maintenance could be a trigger to enlarge the soybean production area and increase national soybean production. The present study was undertaken to evaluate the total phenolic content and antioxidant activity of some improved soybean varieties. The observation was done in three replicates, and the standard deviation was made. The results of the present study showed that total phenolic content and antioxidant activity, as measured by ABTS and DPPH, varied among the soybean seeds tested. The variability of total phenolic content and antioxidant activity was not caused by different seed sizes but by the different genetic background encoding for other phenotypes than the seed size. In general, antioxidant activity is positively correlated to total phenolic content. Demas and Tanggamus showed significantly higher antioxidant activity compared to the rest, including imported soybean. Therefore, Demas and Tanggamus could be used as better food sources for human health.
Free Radical Scavenging Activity of Chromolaena odorata L. Leaves Putri Anni Maulida; Devi Anggraini Putri; Sri Fatmawati
IPTEK The Journal for Technology and Science Vol 30, No 3 (2019)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.233 KB) | DOI: 10.12962/j20882033.v30i3.5409

Abstract

Chromolaena odorata is one of the plants used by the community as traditional medicine. Some of community in Ambon, Indonesia used C. odorata leaves as wound medicine. This study aims to evaluate free radical scavenging activity from fraction of methanol extract of C. odorata. Methanol extract of C. odorata leaves is known have good free radical scavenging activity. The fractionation from methanol extract of the C. odorata leaves obtained A-E fraction, where fractions C, D and E showed inhibitory activity against DPPH radicals (2,2-difenill-1-pikrilhidrazil) with IC50 values 63,95; 64.38 and 202.15 μg/mL. Positive control, which is gallic acid, has an IC50 value of 5.29 μg/mL.
Inhibitory effect of calcium hydroxide combined with Nigella sativa against Enterococcus faecalis Myrna Nurlatifah Zakaria; Yusfien Shabrina Putri; Asih Rahaju; Sri Fatmawati; Arief Cahyanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 4 (2021): December 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i4.p181-185

Abstract

Background: Calcium hydroxide is the gold standard medicament for root canal treatment. Enterococcus faecalis, the primary cause of intraradicular persistent endodontic infection, is often identified even after endodontic treatment. Thymoquinone, an active ingredient of Nigella sativa, has an antimicrobial effect on both gram-negative and positive bacteria, including E. faecalis. Purpose: This study aimed to evaluate the inhibitory effect of calcium hydroxide combined with Nigella sativa extract and determine the best ratio for the combined material. Methods: This is an experimental study comprised of six groups (n = 4 per group) based on the material and its ratio, namely; (1) calcium hydroxide; (2) Nigella sativa extract; and groups of the combination of calcium hydroxide and Nigella sativa extract with a ratio (3) 70:30, (4) 50:50, (5) 30:70, (6) 10:90. The inhibitory effect against E. faecalis was evaluated by the agar well diffusion method in Muller–Hinton agar. Observation of the inhibitory zone was performed on the first, third, and seventh days. The collected data were analysed by a one-way ANOVA and LSD post hoc test. Results: Calcium hydroxide has the highest inhibitory effect, and the combination of Nigella sativa extract with calcium hydroxide ratio 50:50 was second. The inhibitory zone of these two groups was significantly higher than in any other group (p<0.05). Conclusion: Nigella sativa extract combine with calcium hydroxide did not enhance calcium hydroxide’s antimicrobial property against E. faecalis. An equal amount of Nigella sativa and calcium hydroxide is the best combination ratio, with a stable effect for up to seven days.
Antioxidant Activity of Moringa oleifera Extracts Wiwit Denny Fitriana; Taslim Ersam; Kuniyoshi Shimizu; Sri Fatmawati
Indonesian Journal of Chemistry Vol 16, No 3 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.518 KB) | DOI: 10.22146/ijc.21145

Abstract

Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.
New Prenylated Stilbenes and Antioxidant Activities of Cajanus cajan (L.) Millsp. (Pigeon pea) Taslim Ersam; Sri Fatmawati; Dini Nur Fauzia
Indonesian Journal of Chemistry Vol 16, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.492 KB) | DOI: 10.22146/ijc.21158

Abstract

New Prenylated stilbene derivatives, 3-hydroxy-4-prenyl-5-methoxy-stilbene acid (1) and 5,4'-dihydroxy-3-methoxy-2-prenyl-stilbene (2) had been isolated from methanol extract of Cajanus cajan (L.) Millsp. (Pigeon pea), a member of family fabaceae. The aim of this present work was to evaluate its in vitro antioxidant activity using 2,2-diphenyl-1-picryhydrazyl (DPPH) radical-scavenging. Isolation of compounds was achieved by extraction (maceration) and chromatographic technique. The structures of the compounds were determined based on UV, IR, 1H-NMR and 13C-NMR spectra and also by comparing with the previously reported data. Antioxidant activities (IC50) of 1 and 2 were 413.68 μg/mL and 271.53 μg/mL, respectively.
Antioxidant Activity of Syzygium polyanthum Extracts Mutia Devi Hidayati; Taslim Ersam; Kuniyoshi Shimizu; Sri Fatmawati
Indonesian Journal of Chemistry Vol 17, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.1 KB) | DOI: 10.22146/ijc.23545

Abstract

Antioxidant activities of Syzygium polyanthum leaves extracts (methanol, ethyl acetate, dichloromethane and n-hexane) were evaluated by using DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis (3-ethylbenzothiazole-6-sulfonic acid) methods. The methanol extract showed the highest antioxidant activity of DPPH assay among extract with IC50 value of 44.35 mg/mL. In addition, methanol extract also showed the highest antioxidant activity of ABTS assay among extracts with IC50 value of 17.69 mg/mL. This study indicated that the methanol extract of S. polyanthum leaves is potential as antioxidant.
METABOLIT SEKUDER, METODE EKSTRAKSI, DAN BIOAKTIVITAS CABAI (CAPSICUM) Nur Hasanah; Sri Fatmawati
Akta Kimia Indonesia Vol 7, No 1 (2022)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v7i1.11239

Abstract

Cabai merupakan tanaman yang berasal dari Amerika Tengah dan Selatan dengan genus Capsicum. Tanaman genus ini dilaporkan memiliki turunan senyawa capsaicinoid. Capsaicin merupakan senyawa capsaicinoid utama pada cabai yang dapat menimbulkan sensasi panas pada tanaman ini. Sensasi tersebut dapat dimanfaatkan untuk meredakan nyeri sehingga senyawa ini banyak dmanfaatkan sebagai obat analgesik atau penghilang rasa sakit di bidang farmasi. Selain itu, tanaman genus ini juga memiliki kelompok senyawa karotenoid yang berperan sebagai pemberi warna pada buah tanaman tersebut, fenolik, flavonoid, vitamin dan senyawa volatil. Kadar metabolit sekunder cabai ini dipengaruhi oleh pemilihan metode dan kondisi ekstraksi yang digunakan sehingga penelitian terkait ekstraksi cabai ini banyak dilakukan dengan berbagai variasi. Beberapa metode ekstraksi yang digunakan yaitu maserasi, sokletasi, dan refluks hingga ekstraksi modern seperti sonikasi, microwave, subcritical fluid dan supercritical fluid (SFE). Bioaktivitas pada buah cabai yang telah dilaporkan oleh beberapa literatur meliputi antiinflamasi, antimikroba, antioksidan, antidiabetes, antikanker dan analgesik. Meskipun bioaktivitas tanaman ini telah banyak dilaporkan, penelitian lebih lanjut diperlukan untuk mengetahui keamanan, dosis penggunaan dan efek samping dari cabai tersebut. Oleh karena itu, review ini bertujuan untuk memberikan informasi ilmiah tentang Senyawa metabolit sekunder, metode ekstraksi dan bioaktivitas buah cabai yang nantinya diperlukan dalam penelitian selanjutnya.
Antioxidant activity of Momordica charantia extracts Devi Anggraini Putri; Sri Fatmawati
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 12 No 3 (2021): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v12i3.488

Abstract

Momordica charantia is one of the herbaceous plants which widely distributed at tropical and subtropical regions. M. charantia, as known as pare, has been used as Indonesian traditional medicine for the treatment of bitter stomachic, laxative, and anthelmintic. This study aims to investigate the antioxidant activity of M. charantia leaves extracts (the n-hexane, dichloromethane, ethyl acetate, and methanol extracts) by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The result showed that the ethyl acetate extract has the highest DPPH scavenging activity with IC50 value of 233.39 μg/mL. Therefore, this study concluded that ethyl acetate extract of M. charantia leaves is recommended as an antioxidant agent.