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Blood Malondialdehyde, Reproductive, and Lactation Performances of Ewes Fed High PUFA Rations Supplemented with Different Antioxidant Sources Dilla Mareistia Fassah; Lilis Khotijah; Afton Atabany; Ridha Rianti Mahyardiani; Ridha Puspadini; Afi Yulian Putra
Media Peternakan Vol. 38 No. 1 (2015): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.45 KB) | DOI: 10.5398/medpet.2015.38.1.48

Abstract

The aims of this study were to evaluate the effect of vitamin E (vit E) and black tea extract (BTE) as antioxidant sources in high poly-unsaturated fatty acid (PUFA) rations on blood malondialdehyde (MDA) concentrations and the performance of reproduction and lactation of Garut ewes. Twelve ewes on late pregnancy periods were divided into completely randomized design (CRD) with 3 treatments and 4 replicates. The treatments were control: basal diet without antioxidant, vit E: basal diet supplemented with vit E, and BTE: basal diet supplemented with BTE. The results showed that vit E and BTE supplementation did not affect blood MDA concentration of ewes on late pregnancy, lactation periods, and weaning periods. Antioxidant sources supplementation had no effect on ewe’s nutrient intake, pre-lambing live weight change (LWC), and post-lambing average daily gain (ADG). Vit E supplementation had decreased the milk production, but both of antioxidant sources give positive effect on the milk composition. Nevertheless, the supplementation of vit E and BTE increased the twin type of birth. The vit E supplementation resulted higher lambing rate than BTE, although it had the highest mortality rate of twin lamb. BTE also had better lamb weaning weight than vit E. BTE and vit E gave similar results in ewes productivity on lactation period. In conclusion, this study confirmed that BTE was more effective used as antioxidant source than vit E to prevent the oxidative reaction of PUFA. Vit E supplementation on high PUFA ration reduced ewes milk production but it had similar reproduction performance with BTE.Key words: antioxidant, black tea extract, ewes, lactation, reproduction, vitamin E
Efek Pemberian Ekstrak Limbah Teh Hitam sebagai Sumber Antioksidan dal Level Energi-Protein Pakan yang Berbeda terhadap Stabilitas Oksidatif dan Kualitas Daging Ayam Broiler Dilla Mareistia Fassah; Supadmo (Supadmo); Rusman (Rusman)
Buletin Peternakan Vol 36, No 2 (2012): Buletin Peternakan Vol. 36 (2) Juni 2012
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v36i2.1583

Abstract

Penelitian bertujuan untuk mengetahui pengaruh pemberian ekstrak limbah teh hitam (ELT) sebagai sumber antioksidan, dan level energi - protein pakan serta interaksi antara keduanya terhadap stabilitas oksidasi dan kualitas daging ayam broiler. Percobaan ini menggunakan analisis variansi pola faktorial 3 x 3 (3 faktor level pemberian aditif pakan ELT dan 3 tipe pakan) dan perbedaan yang signifikan dilanjutkan uji Duncan’s New Multiple Range Test (DMRT). Tipe pakan yaitu tipe 1: energi - protein rendah, tipe 2: energi - protein sedang, tipe 3: energi - protein tinggi. Tiap perlakuan pakan diberi 3 level pemberian aditif: ELT 0, 500, dan 1.000 mg/kg pakan. Percobaan dilakukan selama 42 hari menggunakan 45 ekor ayam, tiap perlakuan terdiri atas 5 ekor ayam sebagai replikasi. Parameter yang diukur meliputi komposisi kimia daging, kualitas fisik daging, kadar malonaldehyde (MDA), kadar kolesterol, dan komposisi asam lemak daging. Hasil penelitian memperlihatkan bahwa level pemberian ELT dan level energi – protein pakan saling berinteraksi dalam mempengaruhi nilai pH, susut masak dan kadar MDA, total asam lemak jenuh (SFA), total asam lemak mono tidak jenuh (MUFA) serta total asam lemak poli tidak jenuh (PUFA) daging. Pemberian ELT berpengaruh tidak nyata terhadap kualitas kimia daging, tetapi berpengaruh nyata menurunkan pH daging, kadar MDA dan SFA daging (P<0,05), meningkatkan total MUFA dan PUFA daging secara nyata (P<0,05). Level energi - protein pakan tidak menyebabkan perbedaan kualitas kimia daging, tetapi daya ikat air daging, kadar MDA daging, kolesterol daging, total MUFA dan PUFA (P<0,05), menurunkan total SFA (P<0,05). Hasil yang didapat menunjukkan bahwa penambahan ELT sebagai aditif pakan memiliki efek yang baik terhadap stabilitas oksidasi yang tercermin dalam penurunan kadar MDA dan total SFA daging serta meningkatkan total PUFA dalam daging, tanpa menyebabkan perbedaan kualitas daging. (Kata kunci: Ekstrak limbah teh hitam, Antioksidan, Level energi-protein, Stabilitas oksidasi, Kualitas daging)
Pengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak Jenuh Terhadap Profil Darah Induk Domba Laktasi§ (VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATED FATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) Dilla Mareistia Fassah; Lilis Khotijah
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.641 KB)

Abstract

Sunflower oil is a source of rich poly-unsaturated fatty acid (PUFA) mainly Linoleic acid. PUFA on theewes ration was very useful due to its function as the precursors of prostaglandin. It plays an importantrole in the health and reproduction regulation. Early lactation was the critical period where the bodymetabolism is on an increasing stage. However, high level of PUFA on ewe’s lactation ration was also verysusceptible to induce lipid peroxidation which can cause health problems in the affected animals. Thesupplementation of vitamin E as antioxidant is needed to prevent the PUFA from oxidation damage. Theaim of this study was to evaluate the effect of vitamin E supplementation in the rich PUFA diet on theblood profile of lactating ewes. Fifteen ewes on lactation period were used in completely randomizednested design. The level of vitamin E supplementation was 30 IU, 60 IU and 90 IU then the blood profileevaluation was carried out on 28 and 56 days of lactation. The measured variables were haemoglobin,packed cell volume (PCV), erythrocytes, leukocytes, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). The number of erythrocytesof ewes was 5.53-9.85 x 106/mm3, the MCV value was 32.19-46.20 fl and the MCH value was 10.91-17.27pg on day-28. The results showed that Vitamin E supplementation has significant effect on the number oferythrocytes (p<0,05), and the high significant effect on MCV as well as MCH values(P<0.01) of lactationewes blood. The Vitamin E supplementation until 90 IU was able to maintain the ewe’s blood profile onthe lactation periods.
Kecernaan Nutrien Domba Fase Akhir Kebuntingan yang Diberi Ransum Flushing dengan Frekuensi yang Berbeda: Nutrient Digestibility of Local Ewes in Late Pregnancy Phase Fed by Flushing Ration with Different Feeding Frequency Aulia Nurul Saputri; Dewi Apri Astuti; Dilla Mareistia Fassah
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.14-18

Abstract

The aim of this research was to evaluate the nutrient digestibility of late pregnancy local ewes treated with flushing ration and different feeding frequency. Twelve local ewes in the late pregnancy phase (BW 49.96 ± 9.67 kg) were divided on a Randomized Block Design with 4 treatments and 3 replications. The treatments were P1 (without flushing), P2 (flushing at the before and after mating), P3 (flushing at the before and after mating, and middle of pregnancy), and P4 (flushing at the before and after mating, middle, and late of pregnancy). Flushing concentrate containing 2% coconut oil and 5% lemuru fish oil. The variables observed were nutrient consumption and nutrient digestibility. The data were analyzed by using ANOVA, the data with significant differences in the treatment effects were further tested using Duncan Test. The result showed that the treatments significantly affected (p<0.01) consumption of crude fat. Giving the flushing ration at the before and after mating, and mid pregnancy significantly increased (p<0.05) digestibility of dry matter, crude protein, crude fiber, and N-free extract. It can be concluded that the feeding frequency of flushing affects the consumption and digestibility of local sheep at the late stages of pregnancy. Giving flushing ration at the before and after mating, and middle of pregnancy showed the best digestibility of dry matter, crude protein, crude fiber, and N-free extract. Key words: flushing, lemuru fish oil, local ewes, nutrient digestibility
Kecernaan Nutrien dan Status Fisiologis Domba Akhir Kebuntingan dengan Ransum Flushing Minyak Sawit dan Minyak Lemuru: Nutrient Digestibility and Physiological Status of Late Pregnancy Ewe fed with Flushing Diet Containing Palm Oil and Lemuru Oil Lilis Khotijah; M Yasin; Didid Diapari; Dilla Mareistia Fassah
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 19 No. 3 (2021): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.19.3.71-78

Abstract

The objective of this study was to evaluate nutrient digestibility and physiological status of local sheep at late pregnancy fed with flushing diet containing palm oil, lemuru oil, and their combination. In this research, the livestock used were 12 female local sheep at late pregnancy with an average body weight of 43.58 ± 5.57 kg were placed in metabolic cages. This study used a randomized block design (RBD) with 4 treatments and 3 block replications. The treatments were P1 = control ration (30% grass + 70% concentrate), P2 = control ration + 6% palm oil supplementation, P3 = control ration + 3% lemuru oil and 3% palm oil supplementation, P4 = control ration + 6% lemuru oil supplementation. The feed consumption, nutrient digestibility, and physiological status were measured in this study. The data obtained were analyzed using ANOVA. Means values differences were analyzed using Duncan test. The results showed that the addition of palm oil and lemuru oil to the concentrate diet increased (p<0.05) crude fat consumption, dry matter digestibility, and crude fat digestibility, but decreased (p<0.05) Nitrogen free extract consumption. Addition of palm oil and lemuru oil on the concentrate diet had no significant effect on dry matter consumption, crude protein consumption, crude fiber consumption, crude protein digestibility, crude fiber digestibility, Nitrogen free extract digestibility, and TDN value. The treatments did not affect significantly on physiological status. It can be concluded that the use of 6% palm oil in the ration can increase crude fat consumption and crude fat digestibility in pregnant ewes. Key words: digestibility, ewes, flushing, oil, physiological status
Karakteristik Fermentasi Rumen Domba Secara In Vitro dengan Pemberian Maggot Black Soldier Fly yang Dipelihara dengan Ampas Teh dan Ampas Sagu: Characteristics of In-Vitro Sheep Rumen Fermentation of Black Soldier Fly Maggot Reared on Tea Waste and Sago Pulp Dilla Mareistia Fassah; Nella Nurhazizah; Dewi Apri Astuti; Lilis Khotijah
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 3 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.3.111-116

Abstract

The objective of this study was to evaluate the chemical composition of Black Soldier Fly (BSF) maggot grown in tea waste or sago pulp medium and their effects on fermentability and digestibility (in vitro). A randomized block design with 3 dietary treatments and 4 groups of rumen source from different sheep was used in this study. Dietary treatments consisted of P1 = soybean meal protein ration, P2 = BSF maggot meal protein ration grown in tea waste, P3 = BSF maggot meal protein ration grown in sago pulp. The data were analysed using ANOVA followed by Duncan's test. The results show that the NH3 concentration was higher (p<0.05) in treatment containing maggot meal protein grown in tea waste than others. However, each treatment did not have a significant effect on pH, total VFA, dry matter digestibility and organic matter digestibility in vitro. In conclusion, BSF maggot grown in tea waste and sago pulp media can replace soybean meal as a protein source in the ration without any negative effects on in vitro fermentability and digestibility. Key words: black soldier fly, digestibility, fermentability, in vitro, rumen microbes
Blood metabolites profile and Growth Hormone mRNA expression of Peranakan Ongole cattle fed with finishing ration containing Vinasse-molasses Dilla Mareistia Fassah; Nanda Nadhifa Nuraini; Lilis Khotijah; Kokom Komalasari; Sri Suharti; Dewi Apri Astuti; I Komang Gede Wiryawan; Asep Sudarman; Didid Diapari
Livestock and Animal Research Vol 21, No 2 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i2.71312

Abstract

Objective: This study aimed to investigate effects of Molasses replacement with Vinasse-molasses in ration on the blood metabolite profiles and mRNA expression of Growth Hormone 1 (GH1) of finishing Peranakan Ongole (PO) Cattle.Methods: Ten male PO cattle in averaged 209 ± 21 kg of body weight were equally allocated to receive either molasses or Vinasse-molasses dietary treatments. The concentrate diet was contained of either 15% of molasses (control) or Vinasse-molasses. This study observed the nutrient intake, percentage of weight gain, blood metabolite profiles and GH1 mRNA expression of PO cattle.Results: The result showed that replacing molasses with vinasse-molasses reduced (P<0.05) dry matter intake (DMI) and intakes of crude protein (CP), ether extract (EE), crude fiber (CF), and total digestible nutrient (TDN). Nevertheless, Vinasse-molasses inclusion did not change (P>0.05) percentage of weight gain. Vinasse-molasses increased (P<0.05) the blood urea nitrogen level, while it did not affect (P>0.05) the GH1 mRNA levels.Conclusions: It can be concluded that replacing molasses with vinasse-molasses does not change blood metabolite profile, GH1 mRNA expression and performance of PO cattle.  Vinasse-molasses could be applied as energy sources ingredient to replace molasses in finishing cattle feed.
Suplementasi Zn dalam Ransum Mengandung Ampas Teh terhadap Kecernaan Nutrien dan Absorpsi Zn Kelinci Laktasi Lilis Khotijah; Dilla Mareistia Fassah
Jurnal Peternakan Indonesia (Indonesian Journal of Animal Science) Vol 25, No 2 (2023): Jurnal Peternakan Indonesia
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpi.25.2.177-184.2023

Abstract

Mineral mikro Seng (Zn) berperan penting dalam proses reproduksi ternak. Namun demikian, penggunaan pakan mengandung serat tinggi dapat mempengaruhi absorbsi Zn. Penelitian ini mengevaluasi pengaruh level suplementasi Zn pada ransum yang mengandung ampas teh 30% terhadap kecernaan bahan kering dan bahan organik, serta absorpsi mineral Zn pada kelinci periode laktasi. Penelitian ini dilakukan selama 4 bulan menggunakan rancangan acak lengkap pola searah. Dua puluh ekor kelinci dara New Zealand White siap kawin (BB 1,85 ± 0,03 kg) dibagi ke dalam 4 perlakuan pakan, yaitu: R1: ransum komersial (control), R2: ransum ampas the 30%, R3: R2 + Zn 50 ppm, R4: R2 + Zn 100 ppm. Hasil penelitian menunjukkan bahwa level suplementasi dalam ransum mengandung 30% ampas teh sangat nyata meningkatkan (P<0,01) konsumsi Zn dan kadar Zn dalam feses, namun tidak berpengaruh nyata (P>0,05) pada kecernaan bahan kering, bahan organik, dan absorpsi Zn kelinci periode laktasi. Dapat disimpulkan bahwa level suplementasi Zn dalam ransum mengandung ampas teh 30% tidak mempengaruhi kecernaan bahan kering dan bahan organik, serta absorpsi Zn pada kelinci periode laktasi sehingga dapat digunakan sebagai pengganti ransum komersial. 
Pendampingan Aplikasi Teknologi Fermentasi untuk Mendukung Penyediaan Pakan Ruminansia Berkelanjutan di Kecamatan Ciampea, Kabupaten Bogor Dilla Mareistia Fassah; Asep Sudarman; Lilis Khotijah; Didid Diapari; Kokom Komalasari
Madaniya Vol. 5 No. 1 (2024)
Publisher : Pusat Studi Bahasa dan Publikasi Ilmiah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53696/27214834.723

Abstract

Hijauan merupakan pakan utama ruminansia. Ketersediaan hijauan di daerah tropis biasanya berlimpah di musim hujan dan kurang saat musim kemarau. Untuk mengatasi masalah tersebut, maka perlu ada upaya pengawetan pakan ketika hijauan berlimpah sehingga bisa dimanfaatkan ketika musim kemarau. Fermentasi merupakan salah satu teknologi yang dapat digunakan untuk menjaga ketersediaan hijauan pakan. Proses fermentasi hijauan pakan membentuk produk berupa silase. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman dan keterampilan peternak untuk menyediakan pakan hijauan yang berkelanjutan untuk mendukung produksi sapi pedaging. Pelaksanaan kegiatan pelatihan ini bekerja sama dengan Peternakan Kandang Juragan di Kecamatan Ciampea, Kabupaten Bogor. Metode pelaksanaan kegiatan ini meliputi: (1) diskusi, (2) pelatihan, dan (3) pendampingan kepada mitra peternak. Hasil pelatihan menunjukkan bahwa 71,4% peternak menyatakan adanya peningkatan kemampuan dalam menerapkan teknologi fermentasi secara mandiri. Dengan pelatihan dan pendampingan langsung, 85,7% peternak puas akan materi pelatihan yang diberikan, serta 57,1% peternak menyatakan materi pelatihan sangat mendukung keberlangsungan usaha.
Daya Simpan Probiotik Bakteri Asam Laktat asal Larva Black Soldier Fly Terenkapsulasi: Shelf-life of Encapsulated Lactic Acid Bacteria Probiotics from Black Soldier Fassah, Dilla Mareistia; Hairani, Atikah; Meryandini, Anja; Astuti, Dewi Apri; Wiryawan, I Komang Gede
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 22 No. 1 (2024): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.22.1.23-28

Abstract

Lactic acid bacteria as probiotic candidates can be isolated from black soldier fly larvae. This study aimed to develop encapsulated lactic acid bacteria (LAB) probiotics from BSF larvae and to evaluate the effect of encapsulation on probiotic viability during 12 weeks of storage at room temperature and 4°C. The experimental design was a factorial completely randomized design with two treatments: storage temperature and storagetime. The variables observed are the number of cells and the level of cell resistance. The result showed that based on the growth curve of LAB isolates from BSF larvae, they could be harvested at 16 hours of age to be produced as probiotics. Encapsulated LAB probiotics from BSF larvae had a population of lactic acid bacteri (LAB) 107 CFU g-1 and were able to maintain the cell viability of 99.3%. Temperature and storage timesignificantly affect (p<0.05) the viability of encapsulated LAB. It can be conclude that encapsulation was able to optimally maintain the LAB viability at temperature of 4°C for 4 weeks of storage. Key words: black soldier fly larvae, encapsulation, probiotic, storability, viability