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Karakter Morfologi dan Kimia 18 Kultivar Pamelo (Citrus maxima (Burm.) Merr.) Berbiji dan Tanpa Biji Slamet Susanto; Arifah Rahayu; Dewi Sukma; Iswari S. Dewi
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Pamelo is one of the oranges species which have variety of form, size, colour and taste. Most of the pamelo cultivars with seeds, while part of it is seedless. The concumen prefer to chose seedles than with seed because they could consume more. The research proposed to know the morphologycal and chemical characteristics of pamelo with seed and seedless. Characteristication done in RGCI and port harvesting laboratory at IPB to the Pamelo come from Sumedang, Pati, Kudus, Magetan, Aceh and Pangkep (South Sulaewsi) in the period of April 2009 until July 2010. The result of research shown that several pamelo seedless cultivars have pyriform, while other with seed have spheroid form. A few fruit from pamelo seedless cultivars have sweet taste until less taste, with pH of its juice vary from 6.2-6.3, except Jawa cultivar which have pH 4 which its total tertiration acid is 0.47- 0.50 g/g, PTT 9.8- 11.0 ( 0brix), PTT/ATT 19,5-25,3. Vitamine C content 38-48.2 mg/100 g and narigin content from 118,3-1063,2 mg/ml, while pamelo with seed have taste sweet acid, with fruit juice pH 3.7 - 4.7, except "red bali 1", which have pH 6.0, ATT 0,35 - 0,59 g/g, PTT 8.7 - 11.3 ( 0brix), PTT/ATT 16.9 - 24.6, vita mine C content 28.6 - 43.8 and narigin content 55.2 - 461.2 mg/ml.

Perbandingan Pola Pita Isoenzim 15 Aksesi Pamelo (Citrus maxima (Burm.) Merr.) Berbiji dan Tidak Berbiji dan Hubungan Kekerabatannya Arifah Rahayu; Slamet Susanto; Bambang S. Purwoko; Iswari S. Dewi
Jurnal Hortikultura Indonesia Vol. 3 No. 1 (2012): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThere are many pummelo accessions in Indonesia, some of them are seedless. The objective of this work was to compare isoenzyme banding patterns and to assess the genetic similarity of seeded and seedless pummelo accessions. Electrophoresis analysis of proteins extracted from leaf tissues was uti lized to detect polymorphisms i.e. five isoenzymes (esterase (EST), peroxidase (PER), malate dehydrogenase (MDH), acid phosphatase (ACP) and aspartate amino transferase (AAT). Based on principal component analysis, characters having the main role in classifying pummelo accessions were MDH (Rf 0.14 and Rf 0.27) and ACP (Rf 0.24 and Rf 0.33). The accessions showed high range genetic similarity (28.6-94.7%), and at similarity coefficient 0.53 they were classified into seeded and seedless groups. It was concluded that isoenzymes can be used as markers in differentiating seeded and seedless pummelo accessions.Key words: genetic similarity, electrophoresis, marker, principal component analysis, polymorphismABSTRAKIndonesia memiliki banyak aksesi pamelo, baik yang berbiji maupun tidak berbiji. Penelitian ini bertujuan untuk membandingkan pola pita isoenzim dan mengetahui keanekaragaman genetik antar aksesi pamelo berbiji dan tidak berbiji. Analisis isoenzim untuk mendeteksi polimorfisme dilakukan dengan cara elektroforesis menggunakan lima sistem enzim, yaitu esterase (EST), peroksidase (PER), malat dehidrogenase (MDH), asam fosfatase (ACP) dan aspartat amino transferase (AAT). Hasil analisis komponen utama menunjukkan bahwa karakter yang berperan penting dalam pengelompokan aksesi pamelo adalah MDH (Rf 0.14 dan Rf 0.27) dan ACP (Rf 0.24 dan Rf 0.33). Tingkat kesamaan genetik aksesi pamelo berkisar antara 28.6-94.7%, dan pada koefisien kemiripan 0.53 aksesi pamelo dibedakan atas kelompok berbiji dan tidak berbiji. Dengan demikian isoenzim dapat digunakan sebagai penanda dalam membedakan aksesi pamelo berbiji dan tidak berbiji.Kata kunci: kemiripan genetik, elektroforesis, penanda, analisis komponen utama, polimorfisme

Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan Iswari S. Dewi; Gani S. Jawak; Bambang S. Purwoko; M. Sabda
Jurnal Hortikultura Indonesia Vol. 5 No. 1 (2014): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTIn vitro conservation has been applied to many species. However, the suppression of explant growth is essential for extending the duration of conservation. The objective of the research was to study in vitro growth response of pummelo cv. Nambangan to conservation medium containing osmotically active compound (osmoticum) or growth suppressant (retardant). Two sets of experiments were conducted using randomized complete design and replicated three times. In vitro shoot with four leaves from pummelo, namely cultivar Nambangan, were used as the plant materials. The treatment in the first experiment was MS + osmoticum (mannitol 0, 20, 40, and 60 g L-1) and in the second experiment was MS + retardant (paclobutrazol 0, 1, 3 and 5 mg L-1). The resultsindicated that senescence of the leaf was induced by 20, 40, and 60 g L-1of mannitol. The best media in inhibition of growth for pummelo cv. Nambangan was MS + paclobutrazol 1 mg L-1. With this media, plant was inhibited but grew normally with green leaf and root.Keywords: mannitol, minimal growth, paclobutrazol, pummelo ABSTRAKKonservasi in vitro sudah banyak dilakukan pada berbagai spesies. Penghambatan pertumbuhansangat penting bagi lamanya tanaman dapat disimpan. Tujuan penelitian ini adalah mempelajari respon pertumbuhan in vitro pamelo cv. Nambangan terhadap media konservasi mengandung osmotikum atau penghambat pertumbuhan (retardan). Dua percobaan dilakukan terpisah menggunakan rancangan acak lengkap dan diulang 3 kali. Tunas hasil perbanyakan in vitro dengan 4 daun, digunakan sebagai eksplan. Perlakuan pada percobaan pertama adalah MS + osmotikum (mannitol 0, 20, 40, 60 g L-1) dan pada percobaan kedua adalah MS + retardan (paclobutrazol 0, 1, 3,5 mg L-1). Hasil menunjukkan bahwa daun mengalami senesen oleh perlakuan mannitol. Media yang direkomendasikan untuk konservasi pamelo cv. Nambangan adalah MS + paclobutrazol 1 mg L-1. Dengan media tersebut pertumbuhan dihambat, tetapi tetap normal, berakar dengan daun tetap hijau.Kata kunci: mannitol, pertumbuhan minimal, paclobutrazol, pamelo

Induksi Kalus dan Regenerasi Tiga Genotipe Tomat (Solanum lycopersicon L.) melalui Kultur Antera Ratna Ningsih; Bambang S. Purwoko; Muhamad Syukur; Iswari S. Dewi
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others genotypes. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2% while in Tora was 2.1% and Ratna was 0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4%.Keywords: androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah tomat varietas Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2% lebih tinggi dibandingkan Tora (2.1%) dan Ratna (0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4%).Kata kunci: androgenesis, auksin, in vitro, media, sitokinin, tomat

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