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Respon Pertumbuhan Kultur In Vitro Jeruk Besar (Citrus maxima (Burm.) Merr.) cv. Nambangan terhadap Osmotikum dan Retardan Iswari S. Dewi; Gani S. Jawak; Bambang S. Purwoko; M. Sabda
Jurnal Hortikultura Indonesia Vol. 5 No. 1 (2014): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.305 KB) | DOI: 10.29244/jhi.5.1.21-28

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ABSTRACTIn vitro  conservation has been applied to many species. However, the suppression of explant growth is essential for extending the duration of conservation.  The objective of the research was  to study  in  vitro  growth response  of  pummelo  cv.  Nambangan  to  conservation  medium containing osmotically  active  compound  (osmoticum)  or  growth  suppressant (retardant).  Two  sets  of experiments were conducted  using randomized complete design and replicated three times.  In vitro shoot with four leaves from pummelo, namely cultivar Nambangan, were used as the plant materials. The treatment in the first experiment was  MS + osmoticum (mannitol  0, 20, 40,  and 60 g L-1) and  in the  second  experiment  was  MS +  retardant  (paclobutrazol  0,  1,  3  and  5  mg  L-1).  The  resultsindicated that senescence of the leaf was induced by 20, 40, and 60 g L-1of mannitol. The best media in inhibition of growth  for  pummelo cv. Nambangan was MS + paclobutrazol  1  mg  L-1.  With this media, plant was inhibited but grew normally with green leaf and root.Keywords: mannitol, minimal growth, paclobutrazol, pummelo ABSTRAKKonservasi  in  vitro   sudah  banyak  dilakukan  pada  berbagai  spesies. Penghambatan  pertumbuhansangat  penting  bagi  lamanya  tanaman  dapat disimpan.  Tujuan  penelitian  ini  adalah  mempelajari respon  pertumbuhan  in vitro  pamelo  cv.  Nambangan  terhadap  media  konservasi mengandung osmotikum  atau  penghambat  pertumbuhan  (retardan).  Dua percobaan  dilakukan  terpisah  menggunakan  rancangan  acak  lengkap  dan diulang  3  kali.  Tunas  hasil  perbanyakan  in  vitro  dengan  4 daun, digunakan  sebagai  eksplan.  Perlakuan  pada  percobaan  pertama  adalah  MS +  osmotikum (mannitol 0, 20, 40, 60 g L-1) dan pada percobaan kedua adalah MS + retardan (paclobutrazol 0, 1, 3,5 mg L-1). Hasil menunjukkan bahwa daun mengalami senesen oleh perlakuan mannitol. Media yang direkomendasikan untuk konservasi pamelo cv. Nambangan  adalah  MS + paclobutrazol 1 mg  L-1. Dengan media tersebut pertumbuhan dihambat, tetapi tetap normal, berakar dengan daun tetap hijau.Kata kunci: mannitol, pertumbuhan minimal, paclobutrazol, pamelo
Oil Palm Seed Coating with Enriched Trichoderma asperellum (T13) to Suppress Infection of Ganoderma boninense Pat. Gani Jawak; Eny Widajati; Endah Retno Palupi; Nutrita Toruan Mathius
Jurnal Perbenihan Tanaman Hutan Vol 6, No 2 (2018): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.582 KB) | DOI: 10.20886/bptpth.2018.6.2.121-132

Abstract

The attack Ganoderma boninense can caused stem rot of oil palms that occur at all stage of plant growth. Trichoderma asperellum endophytic can suppressed the attack of Ganoderma in a nursery by utilizing seed coating technology. The aim of this study was to determine the best formula of seed coating materials which is compatible with T. asperellum (T13). The first experiment consisted of two phases, namely, the first phase was testing the effectiveness and compatibility of T. asperellum through a mixing technique of  T. asperellum suspension with coating material. The best three result on the first phase (25 percent arabic gum, 1 percent CMC, and 3percent arabic gum + 1 percent gypsum) were used in the second phase of the experiment, which was testing the effectiveness and compatibility of T. asperellum through soaking technique in T. asperellum suspension that continued by coating. The three best result types of formulas from the second phase (1 percent CMC, 1.5 percent CMC, and 4,5 percent arabic gum + 1,5 percent gypsum) were used for the second experiment, namely testing the resistance of oil palm seed on Ganoderma. The results show that 25 percent arabic gum, 1 percent carboxy methyl cellulose (CMC), 1 percent arabic gum + 1 percent gypsum have a potency as coating of materials. The best formula for seed coating is soaking with T. asperellum + coating 1 percent CMC, 1.5 percent  CMC and arabic gum 4.5 percent + gypsum 1.5 percent. In addition, soaking seeds with T. asperellum + coating 1.5 percent CMC can enhance the ability of seed to grow up to 16.67 percent compared to the control, but did not effective in suppressing G. boninense infection during pre-nursery stage.
Konservasi In Vitro Tanaman Jeruk Besar (Citrus maxima (Burm.) Merr.) Kultivar Srinyonya Menggunakan Osmotikum dan Retardan Iswari S Dewi; Gani Jawak; Ika Roostika; Muhammad Sabda; Bambang S Purwoko; Widiati H Adil
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p84-90

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In Vitro Conservation of Pomelo (Citrus maxima (Burm.)Merr.) cv Srinyonya Using Osmoticum and Retardant.Iswari S. Dewi, Gani Jawak, Ika Roostika, M. Sabda,Bambang S. Purwoko, and Widiati H. Adil. Pomelo is anunderutilized citrus fruit with a potential for commercialization.Only some cultivars have been conserved ex situ, suchas in home yards or in botanical gardens. Such collectionsare vulnerable to biotic and abiotic hazards. The goal of theexperiment was to study the effect of osmoticum (sorbitol)and retardant (ancymidol) on in vitro growth of pomelo.Four-leaf in vitro shoots of pomelo cultivar Srinyonya wereused as plant materials. Murashige-Skoog (MS) medium wasused as the basal medium for the culture. The trial wasarranged in a completely randomized design with threereplications. The treatments consisting of MS + sorbitol (0,20, 40, and 60 g/l) and MS + ancymidol (0, 1, 3, and 5 mg/l).The results indicated that based on plant height, number ofnew leaves, and visual plant architecture, sorbitol treatmentsfrom 20-60 g/l retard the growth of the pomelo plant significantly.On the other hand, ancymidol did not inhibit thepomelo growth significantly, but it was a suitable osmoticumfor improvement of in vitro plant vigor, increasing greencolor of leaf, and increasing root initiation. Leaf senescenceof in vitro plants cultured on media containing sorbitol 40and 60 g/l began 20 week after storage. The best medium forconservation of pomelo cv Srinyonya was MS + 20 gsorbitol/l.
Pendugaan Kemunduran Benih dengan Uji Fisiologi dan Biokimiawi Gani Jawak; Eny Widajati; Devi Liana; Tri Astuti
Savana Cendana Vol 7 No 04 (2022): Savana Cendana (SC) - October 2022
Publisher : Fakultas Pertanian, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.083 KB) | DOI: 10.32938/sc.v7i04.1921

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The seed viability test is one of the routine tests carried out in the seed certification process. Decreasing the viability of seeds can be done biochemically and physiologically. This research aimed to study of seeds deterioration through physiological and biochemical tests. This study used two lots of long bean seeds (high and low lot). Physiologically, the seed viability test was carried out by means of germination test and biochemical by means of respiration test using tetrazolium (TTZ) staining technique and titration method. The experiment designed by completely randomized design one factor. Data were analyzed by anova test and Pearson correlation. The results of research showed that the seed lot treatment was significantly on the tetrazolium test and the total dry weight of sprouts, while the vigor index, seed germination, and respiration index measurements did not show significant. There was a high positive correlation in the high respiration rate of high seed lots and low seed lot seeds on total dry weight of germination, germination, vigor index, and tetrazolium test. Therefore, biochemical tests with TTZ and respiration with titration methods can be used as alternatives to predict rapid deterioration of seeds.
Pemanfaatan Sampah Organik untuk Budidaya Sayuran Organik dengan Sistem Vertikultur Bakti Nur Ismuhajaroh; Hilda Susanti; M. Laily Qadry Sukmana; Gani Jawak; Juharni Juharni; Muhammad Ihsan Fadhiel; Andi Rahman Halim
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 3, No 3 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v3i3.12005

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Pemenuhan kebutuhan gizi khususnya sayuran yang sehat di masyarakat perkotaan memerlukan biaya yang tinggi. Ketersediaan lahan yang sempit dan kurangnya pengetahuan menyebabkan rendahnya minat budidaya tanaman masyarakat perkotaan. Pemanfaatan sampah organik limbah rumah tangga untuk budidaya sayuran secara organik dengan sistem vertikultur dapat meningkatkan kemampuan masyarakat dalam mengelola sampah rumah tangga dan produksi sayuran sehat.  Kegiatan ini dilaksanakan di RT. 40 dan RT. 47, RW. 07 Kelurahan Guntung Manggis, Kecamatan Landasan Ulin, Kota Banjarbaru, Kalimantan Selatan. Tahapan kegiatan yang dilakukan adalah sosialisasi, praktik pembuatan demplot, dan monitoring. Pelaksanaan kegiatan ini secara berkelanjutan akan meningkatankan kondisi lingkungan, memenuhi kecukupan gizi rumah tangga dan meningkatkan pendapatan keluarga.
Pematahan dormansi benih cabai lokal tiung tanjung asal tabalong Kalimantan Selatan Hasimi, Muhammad Hasbi; Agustina, Eva; Miskiah, Nur Yohaniz; Fadhiel, Muhammad Ihsan; Nadia, Nadia; Jawak, Gani
Jurnal AGRO Vol 11, No 1 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/35866

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Tiung Tanjung seeds pepper are believed to have dormant properties that can be detrimental to farmers during planting. The aim of this study was to find out the correct method of breaking the dormancy on Tiung Tanjung pepper. The study was designed with a two-stage nested design, the first stage was the seed storage time of 1, 3, 5, 7, 9, and 11 weeks. The second stage was a dormant breakdown method consisting of 8 treatments namely control, aquades, warm water (40 °C), ionic water, IAA 100 ppm, IAA 200 ppm, KNO3 0,1% and KNO3 0.5%. Each unit of experiment used 3 repetitions with 25 seeds planted using Top of Paper method (TP). Parameters observed were the vigor index, growth speed, germination,maximum germination potential, fresh seed, seed mortality rate, and growth performance. The results of the study showed that the treatment of Tiung pepper seed immersed in 0.5% KNO3 for 24 hours was able to break the dormancy at 7 weeks after storage with germination values increased to 80%. Treatment with 0.1% KNO3 could break the dormancy in the 9th week. Dormancy breakdown treatments with aquades, warm water (40 °C), ionic water, IAA 100 ppm, and IAA 200 ppm had not been able to break the dormancy of Tiung pepper seeds up to 11 weeks of storage. Benih cabai Tiung Tanjung diyakini memiliki sifat dormansi yang dapat merugikan petani saat penanaman. Tujuan dari penelitian ini adalah untuk mengetahui metode pematahan dormansi yang tepat pada cabai Tiung Tanjung. Penelitian dilaksanakan dengan rancangan tersarang dua tingkat, tingkat pertama adalah lama masa simpan benih yaitu 1, 3, 5, 7, 9, dan 11 minggu simpan dan tingkat kedua adalah metode pematahan dormansi yang terdiri dari 8 perlakuan yaitu kontrol, akuades, air hangat (40 °C), air ion, IAA 100 ppm, IAA 200 ppm, KNO3 0,1% dan KNO3 0,5%. Setiap satuan percobaan menggunakan 3 ulangan. Setiap ulangan menggunakan 25 benih yang ditanam dengan metode uji di atas kertas (UDK). Parameter yang diamati adalah indeks vigor, kecepatan tumbuh, daya berkecambah, potensi tumbuh maksimum, benih segar tidak tumbuh, dan tingkat kematian benih, dan performa kecambah. Hasil penelitian menunjukkan bahwa perlakuan perendaman benih cabai Tiung dalam KNO3 0,5% selama 24 jam mampu mematahkan dormansi pada 7 minggu setelah simpan dengan nilai daya berkecambah mencapai 80%. Perlakuan dengan KNO3 0,1% dapat mematahkan dormansi pada minggu ke-9. Perlakuan pematahan dormansi dengan akuades, air hangat (40 °C), air ion, IAA 100 ppm dan IAA 200 ppm belum mampu mematahkan dormansi benih cabai Tiung hingga 11 minggu simpan.
Pelatihan Budidaya Tanaman secara Hidroponik kepada Tenaga Penyuluh dan Kelompok Tani Kecamatan Bumi Makmur, Kabupaten Tanah Laut: Hydroponic Plant Cultivation Training for Extension Workers and Farmer Groups in Bumi Makmur District, Tanah Laut Regency Jawak, Gani; Ismuhajaroh, Bakti Nur; Rusmayadi, Gusti; Juharni, Juharni; Wahdah, Raihani; Langai, Bambang Fredrickus; Fadhiel, Muhammad Ihsan; Rambe, Mikhael Wesley; Nababan, Endang Mayjela Enjelika; Yakub, Yakub
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 1 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i1.8253

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Bumi Makmur District is located in a swampy area with the main livelihood being rice cultivation. Vegetables cannot be produced in this region because the swamp soil is acidic and pyrite. One of the solutions offered to meet the needs of vegetables is hydroponic cultivation. Hydroponic training was carried out to increase public knowledge about the cultivation of vegetable crops with hydroponics by socialization and practice of wick system hydroponics. The training participants were Field Agricultural Extension Workers and Farmer Groups, carried out in the hall of the Bumi Makmur Agricultural Extension Center. The number of training participants was 24 people, consisting of 20 men and 4 women. All of the training participants were >18 years old with education levels ranging from junior high school to Master's level. The trainees who had heard the term hydroponics through the internet, radio/television, print media, family, and school were 91.67%. Participants who had cultivated vegetable crops with hydroponics were only 4.17% and planned to cultivate plants hydroponically was 83.33%. A total of 93.83% of participants had never and 95.83% were interested in participating in hydroponic training. The final survey showed that 95.83% of participants wanted to take part in advanced training on hydroponic systems and techniques with hands-on practice methods and a training duration of 2-3 days. Only 58.33% of participants rated hydroponic training as very important. The results of the basic ability test about the participants' knowledge of hydroponics showed an increase in the average score from the initial 50.00 to 68.7.