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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Microbiology Indonesia Indonesian Journal of Chemistry Journal of Tropical Crop Science Makara Journal of Science Jurnal Tumbuhan Obat Indonesia
. TRIVADILA
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health

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Aktivitas urikase yang dihasilkan dari berbagai sel lactobacillus plantarum dan parameter kinetikanya Dyah Iswantini; Novik Nurhidayat; . Trivadila; Eka Mardiah
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 3 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1251.128 KB)

Abstract

Uric acid concentration could be determined by spectrophotometry method. Uric acid was oxidized into allatonin in the presence of uricase and calculated by measuring the decrease of uric acid absorbance at 293 nm. These uricase were obtained from cells of Lactobacillus plantarum. L. plantarum K. Mar. E was isolated from passion fruit skin and L. plantarum Mgs. Psmb and Mgs. Bst from mangosteen. This research was conducted to observe the activity and kinetics of uricase from various cells of L. plantarum by spectrophotometric method. The plate assay method indicated that L. plantarum produced uricase, based on the clear zone about 0,2 mm on glucose yeast peptone medium contained 0,2% uric acid. The optimum condition of uricase activity from the three different sources occured in physiological condition. Uricase activity generated from cells of L. p/antarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 0,1073; 0,0867; and 0,0842 U/ml, respectively. The kinetic parameters for uricase, determined with uric acid as the substrate. Vmax produced by L. plantarum K. Mar. E, Mgs. Psmb, and Mgs. Bst were 1,3635; 0,0316; and 0,0418 U/ml of bacterial culture, respectively and KM 0,1541; 0,0061; and0,0054 mM, respectively. Uricase activity in various bacterial cells of L. plantarum was stable until the second day.
Penentuan kinetika urikase dari sel bacillus subtilis, B. megaterium, dan B. cereus Dyah Iswantini; Novik Nurhidayat; . Trivadila; Adayani Nurjayanti
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Uric acid concentration could be determined based on the oxidation of uric acid into alantoin in the presence of uricase. Determination of uric acid concentration is needed to diagnose the occurrence of kidney disease in gout patients. The aim of the research was to study the kinetics of uricase in Bacillus subtilis, B. megaterium, and B. cereus cells using spectrophotometry method by measurement the decrease of uric acid absorbance at 293 nm. The optimum conditions of uricase activity from the three bacterial cells occured in physiological conditions and uricase activity was stable until the second day. The values of maximum velocity (Vmax) for B. subtilis, B. megaterium, and B. cereuswere 0.1642, 0.0824, and 0.0412 mM/min, respectively. The values of Michaelis-Menten constant (Km) for B. subtilis, B. megaterium and B. cereus were 0.0003, 0.0036, and 0.0036 mM, respectively. The value of catalytic constant (kkat) for B. subtilis, B. megaterium, and B. cereus were 410.5000, 171.6667, and 257.5000 mM/min ml 00=1, respectively. Based on these results, among all bacteria tested, the highest of uricase binding with substrate in B. substilis cells was observed because of the smallest of Km value and greatest of Vmax and kkat .
Aktivitas NADP(H) Oksidoreduktase pada Kultur Sel Kina (Cinchona ledgeriana Moens) Terelisitasi Maulidiyah Utami; Diah Ratnadewi; Dyah Iswantini; Trivadila Trivadila
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 4 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.4.540

Abstract

Cinchona ledgeriana Moens is an industrial plant producing secondary metabolite quinoline alkaloids. To maintain and moreover, to increase the quinoline production especially quinine, in vitro culture system through cell culture could be a potential alternative. If the use of elicitor in cell culture can increase the production of a secondary metabolite, the activity of the enzymes involved in the biosynthetic pathway of the secondary metabolite in question might be increasing. This study aimed to examine the activity of NADPH oxidoreductase in the elicitated cell culture of C. ledgeriana and to evaluate the correlation between the activity of this enzyme and the level of quinine production. The cell cultures of Cinchona were treated with abscisic acid (ABA) or paclobutrazol (PBZ), combined with sucrose, sorbitol, or mannitol in Wood Plant (WP) media, for 7 weeks on a shaker. The quinine concentration was determined using high-performance liquid chromatography (HPLC) and the enzyme activity was measured using fluorometry. The results showed that the highest enzyme activity was found in the P7M cells (PBZ 7 mg/L + mannitol 5.3 g/L + sucrose 20 g/L), followed by the A3S cells (ABA 3 mg/L + sorbitol 5.3 g/L + sucrose 20 g/L). These results correspond to their production level of the quinine alkaloids. The lowest enzyme activity was found in the cultures without elicitor. The increase of NADP(H) enzyme activity in the P7M and A3S treatments were 13.5 and 8.5%, respectively, compared to that in the control cells. Keywords: elicitation, fluorometry, NADP(H) oxidoreductase, quinoline alkaloid
Glucose Biosensor Using Selected Indonesian Bacteria DYAH ISWANTINI; NOVIK NOVIK; . TRIVADILA
Microbiology Indonesia Vol. 5 No. 1 (2011): March 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.954 KB) | DOI: 10.5454/mi.5.1.2

Abstract

Microbial glucose sensors have been developed using Escherichia coli bacterial strains from Japan. However, there is interest in developing local bacteria as glucose sensors in Indonesia. In this research, the stability and the potential of a selected number of Indonesian bacteria as glucose biosensors was explored. Results of this study indicate that three of them, E. coli, Bacillus subtilis, and Thermus filiformis exhibit properties of high viability and stability at high temperature (30-60 ºC). Spectrophotometrical and electrochemical measurements showed significant absorbance values and highly stable current features for E. coli as indicated by its high capacity to produce glucose dehydrogenase. E. coli, B. subtilis, and T. filiformis produced currents of 3.25 µA, 0.2 µA, and 0.02 µA respectively, and E. coli also produced a much higher activity of glucose dehydrogenase. Electrochemical measurement using E. coli-modified carbon paste electrode allowed the determination of glucose concentration of up to 20 mM. Therefore, Indonesian E. coli has a high stability and can be used as a glucose biosensor
Inhibition Kinetics of Sida rhombifolia L. Extract Toward Xanthine Oxidase by Electrochemical Method Dyah Iswantini; Muammar Yulian; Sri Mulijani; Trivadila Trivadila
Indonesian Journal of Chemistry Vol 14, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.064 KB) | DOI: 10.22146/ijc.21270

Abstract

Sida rhombifolia L. is a traditional medicinal plant that has been known with potential as antigout. The previous research suggested that flavonoids crude extract of S. rhombifolia had an inhibitory activity toward xanthine oxidase by 71% and a spectrophotometric measurement showed that the type of flavonoids crude extract inhibition was a competitive inhibition. The purpose of the research was to investigate the type of inhibition kinetic of S. Rhombifolia’s ethanol extract by electrochemical method and to compare the measurements of linearity and sensitivity between spectrophotometric and electrochemical methods. The results showed that the yield of S. Rhombifolia’s ethanol extract was 9.82% with the inhibitory activity ranging from 13.64% to 82.69% (5.00-200 mg L-1) and IC50 value was 91.15±5.74 mg L-1. Allopurinol as a control showed the inhibitory activity of 15.26-70.95% (0.10-4.00 mg L-1) and IC50 value was 2.45±2.21 mg L-1. Inhibition kinetics of the ethanol extract caused a KM increase and unchange of VMAX. Based on the data, the type of inhibition kinetics was a competitive inhibition, with an inhibitor affinity (α) value of 3.18. Linearity of xanthine oxidase activity assay by electrochemical and spectrophotometric methods showed the range of 0.01-1.00 mM (R2 = 0.978) and 0.05-0.70 mM (R2 = 0.977) respectively. The sensitivity of electrochemical method was reported higher (0.95 μA mM-1) than the spectrophotometric method (0.007 min-1).
Sintesis Komposit Zeolit X/Oksida Perak dan Tembaga Melalui Reaksi Tollens Serta Aplikasinya Sebagai Adsorben: Synthesis Zeolite X/Silver and Copper Oxide Composite via Tollens Reactions and Its Application as Adsorbent Hasanuddin Rizal; Zaenal Abidin; Trivadila; Nurul Hiedayati
Jurnal Ilmu Tanah dan Lingkungan Vol 24 No 2 (2022): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.24.2.87-95

Abstract

Zeolit adalah mineral aluminosilika berpori mikro sehingga memiliki permukaan yang luas. Permukaannya yang luas menyebabkan zeolit umum digunakan sebagai adsorben. Zeolit X termasuk dalam struktur Faujasit dan biasa mengikat kation natrium. Kation yang terikat pada zeolit dapat ditukar dengan kation lainnya, seperti kation logam transisi. Kation logam transisi seperti [Ag(NH3)2]+ biasa digunakan sebagai pendeteksi gugus aldehida atau biasa dikenal sebagai pereaksi Tollens. Penelitian ini bertujuan menyintesis komposit adsorben zeolit X/oksida perak dan zeolit X/oksida tembaga melalui reaksi Tollens. Komposit dikarakterisasi menggunakan scanning electron microscope menunjukkan permukaan milik komposit lebih kasar dari pada permukaan zeolit X. Bahan yang digunakan sebagai model untuk adsorpsi ialah, gas amonia. Komposit yang dihasilkan ada tiga jenis berdasarkan tahapan penambahan zeolit pada proses sintesis, yaitu setelah penambahan prekursor (komposit 1), setelah penambahan NH4OH (komposit 2), dan setelah pemanasan (komposit 3). Komposit yang dihasilkan mampu meningkatkan adsorpsi amonia hingga 2 kali lipat daripada zeolit X.
Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics Rut Novalia Rahmawati Sianipar; Komar Sutriah; Dyah Iswantini; Trivadila; Suminar Setiati Achmadi
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.1-20

Abstract

The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.
PHENOLIC AND FLAVONOID PRODUCTION, PHYTOCHEMICAL PROFILE, AND ANTIOXIDANT CAPACITY OF Adenostemma platyphyllum AT DIFFERENT CONCENTRATIONS OF HYDROPONIC SOLUTIONS : Produksi Fenolik dan Flavonoid, Profil Fitokimia, dan Kapasitas Antioksidan Adenostemma platyphyllum pada Konsentrasi Larutan Hara Hidroponik Berbeda Tamsin, Aqlia Hanna Nurfatiha; Batubara, Irmanida; Ridwan, Taopik; Trivadila; Aziz, Sandra Arifin
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.552

Abstract

Adenostemma platyphyllum is considered a weed with various benefits but has not been widely cultivated. This work aims to obtain the optimum concentration of a nutrient solution (AB-mix) to produce optimum phenolic and flavonoid levels, antioxidant capacity and metabolite profiling in A. platyphyllum using a hydroponic wick system. Different AB-mix nutrient solution concentrations were employed in a one-factor randomized block design. The highest total phenolic, flavonoid concentrations and antioxidant capacity were found in plants without additional nutrient solutions. However, the highest phenolic and flavonoid productivity was found in plants with a nutrient solution concentration of 1100 mg/L. The phenolic/flavonoid group compounds were successfully identified by separating the components using thin-layer chromatography. The intensity of the bands produced from each treatment was quite varied. The untreated plant produced thicker phenolic/flavonoid bands than the other treatments. This was supported by the heatmap pattern produced by the untreated ones, which had high color intensity. Therefore, the optimum concentration of nutrient solution to produce optimum phenolic, flavonoid levels, and antioxidant capacity in A. platyphyllum cultivation by hydroponic wick system was the concentration of 1100 mg/L.
Synthesis, Characterization, Antibacterial Activity, and Potential Water Filter Application of Copper Oxide/Zeolite Composite Kamila, Elfa Aida; Abidin, Zaenal; Arief, Irma Isnafia; Trivadila, Trivadila
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

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Abstract

Detection of E. coli bacteria in water samples can indicate the presence of other bacterial contamination in feces. Bacterial contamination can be treated with antibiotics, but excessive use of antibiotics leads to the emergence of multiresistant bacteria. Therefore, alternative antibacterial agents must be explored. Copper ion/zeolite composite has been widely studied as an antibacterial agent. However, the released copper ions accumulate in water and are toxic to humans; this can be prevented by converting copper ions into copper oxides (CuO and Cu2O). In this study, copper oxide/zeolite composite is synthesized and characterized using the heating method, followed by evaluation of its effect, antibacterial activity, and potential application as a water filter. The results showed that heat treatment and combination of zeolite with copper did not change the composition of zeolite, rather impurities were reduced from natural zeolites. Antibacterial activity test against E. coli showed that the copper oxide/zeolite composite possessed good antibacterial activity and exhibited better potential as a water filter compared with copper/zeolite. The copper ion released from the synthesized materials was under 2 mg/L, indicating that it is safe for use provided the minimum contact between the sample and water for less than 20 min or 50–60 min.
Sintesis Granul Komposit Zeolit-Geopolimer dan Adsorpsinya pada Metilen Biru Salsabila, Haditsah; Arief, Irma Isnafia; Trivadila; Abidin, Zaenal
Jurnal Ilmu Tanah dan Lingkungan Vol 26 No 1 (2024): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.26.1.12-20

Abstract

Indonesia merupakan salah satu produsen industri tekstil terbesar di Asia Tenggara. Produksi barang tekstil yang tinggi dan kurang memadainya proses pengelolaan limbah cair tekstil membuat Indonesia menjadi negara dengan limbah cair industri tekstil terbesar di Asia Tenggara. Salah satu teknik pengelolaan limbah pewarna yakni dengan menggunakan bahan adsorben seperti zeolit. Pada penelitian ini, zeolit dikompositkan dengan geopolimer dan dibentuk menjadi granul agar memiliki kemampuan adsorpsi dan ketahanan yang tinggi. Komposit disintesis dengan mencampurkan zeolit, metakaolin, dan natrium silikat (Na2SiO3), kemudian dikeringkan dalam oven pada suhu 100 °C selama 2 jam. Sampel-sampel yang digunakan kemudian diuji adsorpsi pada metilen biru. Prekursor sampel dikarakterisasi menggunakan XRD serta SEM. Hasil pengujian adsorpsi menunjukkan bahwa komposit granul zeolit-geopolimer memiliki kemampuan adsorpsi sebesar 52,68 mg/g untuk granul kecil dan 20,85 mg/g untuk granul besar sehingga dapat diaplikasikan pada lingkungan perairan.
Co-Authors Adayani Nurjayanti Anggia Murni Diah Ratnadewi Dyah Iswantini Eka Mardiah Hasanuddin Rizal Irma Isnafia Arief Irmanida Batubara Kamila, Elfa Aida Komar Sutriah M. Rafi Maulidiyah Utami Muammar Yulian NOVIK NOVIK Novik Nurhidayat Nurul Hiedayati Rut Novalia Rahmawati Sianipar Salsabila, Haditsah Sandra Arifin Aziz Sri Mulijani SUMINAR SETIATI ACHMADI Tamsin, Aqlia Hanna Nurfatiha Taopik Ridwan Zaenal Abidin