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PENGGUNAAN JAMUR ANTAGONIS Trichoderma sp. dan Gliocladium sp. UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) Ramadhina, Arie; Lisnawita, Lisnawita; Lubis, Lahmuddin
Jurnal Agroekoteknologi Vol 1, No 4 (2013)
Publisher : Universitas Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jaet.v1i4.4352

The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams of Gliocladium sp., 18 grams of Gliocladium sp., 24 grams ofGliocladium sp., and three repetitions. The result showed that the worst-highest disease was incontrol with 10 grams of F. oxysporum (2.60%) and the lowest disease was in control, 18 grams ofTrichoderma sp., 24 grams of Trichoderma sp., 18 grams of Gliocladium sp., and 24 grams ofGliocladium sp. (0.71%). The accident of the highest disease was in 10 grams of F. oxysporum(5.01%) and the lowest was in control, 18 grams of Trichoderma sp., 24 grams of Trichoderma sp.,18 grams of Gliocladium sp., and 24 grams of Gliocladium sp. (0.71%). The largest number ofleaves was in 24 grams of Trichoderma sp. (36 leaves), and the smallest was in 10 grams of F.oxysporum (29 leaves). The highest plants was in 24 grams of Gliocladium sp. (40.20 cm) and thelowest in 10 grams of F. oxysporum (37.26 cm). The largest number of F. oxysporum colonies in 10grams of F. oxysporum (8.86%). The highest production was in 24 grams of Trichoderma sp. (2.34tons/ha) and the lowest in 10 grams of F. oxysporum (1.56 tons/ha). The test of antagonism fungusof Trichoderma sp. and Gliocladium sp. on F. oxysporum indicates that the growth of both types ofantagonism is faster so that F. oxysporum tends to keep away from antagonism in the medium of thelaboratory.Keywords: Trichoderma sp., Gliocladium sp., Fusarium oxysporum, antagonism

Aplikasi Cendawan Endofit terhadap Perkembangan Populasi Nematoda Radopholus similis pada Pisang Barangan Lisnawita Lisnawita; Ahmad Rafiqi Tantawi; Mukhtar Iskandar Pinem
Jurnal Fitopatologi Indonesia Vol 9 No 5 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Radopholus similis is a major constraint to banana production in the world including Indonesia and growers have relied on nematicides to manage yield losses. The use of endophytic fungi is one method that may reduce the need for nematicides, however little is known on the effective application method of endophytic fungi to control R. similis. The aim of this research was to find out an effective application method of endophytic fungi to reduce R.similispopulation on banana. Fifteen isolates of endophytic fungi originated from banana corm and root collected from banana plantation in North Sumatera were applied to Barangan cultivar by sowing and deeping methods. The isolates was contained of 11 isolates of Fusarium sp. (2BSTMHMM, 3ASTMHP, 5ASP, 1ASU, 4BSP, 4BJP, 5BKJP, 4BSU, 1BJP, 2 BSP, and 4BKJP) and 4 isolates ofTrichoderma sp. (2BSTMHP, 4BSTMHP, 2BSPH, and 2BSTMHH). All of these endophytic fungi were able to suppress the population of R. similis and they had potential to promote plant growth.

Sebaran Nematoda Sista Kentang di Wonosobo dan Banjarnegara, Jawa Tengah Dani Sutanta Syafii; Lisnawita Lisnawita; Hasanudin Hasanudin
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Distribution of Potato Cyst Nematode in Wonosobo and Banjarnegara, Central JavaPotato cyst nematode (PCN), Globodera spp., is the primary pathogens that infect potato plants and has been distributed world wide. This study aimed to determine the geographic distribution and species of PCN that infect potato plants in Wonosobo and Banjarnegara, Central Java through a survey, morphological and molecular approaches. Morphological characters were observed among cyst (fenestra diameter, distance from anus to the fenestra, and the Graneks ratio, eggs (length and width of eggs), and the juvenile stage 2 (J2) (length and width of the body, the length and width of the head, the length of the stylet, knob type, as well as the length of the tail). While the molecular identification by polymerase chain reaction is based on the amplification of the internal area transcribed spacers using G. rostochiensis PITSr3 specific primer, G. pallida PITSp4 specific primer and universal nematode primer ITS5. Twelve isolates of PCN, i.e. 5 isolates from Wonosobo and 7 isolates from Banjarnegara were used in this study. The results showed that all isolates were identified as single species, G. rostochiensis.

PENGGUNAAN JAMUR ANTAGONIS Trichoderma sp. dan Gliocladium sp. UNTUK MENGENDALIKAN PENYAKIT LAYU FUSARIUM PADA TANAMAN BAWANG MERAH (Allium ascalonicum L.) Arie Ramadhina Arie Ramadhina; Lisnawita Lisnawita; Lahmuddin Lubis
Jurnal Agroekoteknologi Vol 1, No 3 (2013)
Publisher : Universitas Sumatera Utara

The use of antagonism fungus of Trichoderma sp. and Gliocladium sp. for controlling wilt(Fusarium oxysporum) in red onion plants. The aim of the research was to know the effectiviness ofantagonism fungus of Trichoderma sp. and Gliocladium sp. in controlling wilt in red onion plants.The research used non-factorial RAK (random group design) with eight treatments: control, 10grams of F. oxysporum, 12 grams of Trichoderma sp., 18 grams of Trichoderma sp., 24 grams ofTrichoderma sp., and 12 grams of Gliocladium sp., 18 grams of Gliocladium sp., 24 grams ofGliocladium sp., and three repetitions. The result showed that the worst-highest disease was incontrol with 10 grams of F. oxysporum (2.60%) and the lowest disease was in control, 18 grams ofTrichoderma sp., 24 grams of Trichoderma sp., 18 grams of Gliocladium sp., and 24 grams ofGliocladium sp. (0.71%). The accident of the highest disease was in 10 grams of F. oxysporum(5.01%) and the lowest was in control, 18 grams of Trichoderma sp., 24 grams of Trichoderma sp.,18 grams of Gliocladium sp., and 24 grams of Gliocladium sp. (0.71%). The largest number ofleaves was in 24 grams of Trichoderma sp. (36 leaves), and the smallest was in 10 grams of F.oxysporum (29 leaves). The highest plants was in 24 grams of Gliocladium sp. (40.20 cm) and thelowest in 10 grams of F. oxysporum (37.26 cm). The largest number of F. oxysporum colonies in 10grams of F. oxysporum (8.86%). The highest production was in 24 grams of Trichoderma sp. (2.34tons/ha) and the lowest in 10 grams of F. oxysporum (1.56 tons/ha). The test of antagonism fungusof Trichoderma sp. and Gliocladium sp. on F. oxysporum indicates that the growth of both types ofantagonism is faster so that F. oxysporum tends to keep away from antagonism in the medium of thelaboratory.Keywords: Trichoderma sp., Gliocladium sp., Fusarium oxysporum, antagonism

INDEKS KEANEKARAGAMAN JENIS SERANGGA PADA PERTANAMAN KELAPA SAWIT (Elaeis guineensis Jacq.) DI KEBUN HELVETIA PT. PERKEBUNAN NUSANTARA II Gevit R Tambunan; Mena Uly Tarigan; Lisnawita Lisnawita
Jurnal Agroekoteknologi Vol 1, No 4 (2013)
Publisher : Universitas Sumatera Utara

Insect Species Diversity Index in Oil Palm (Elaeis guineensis Jacq.) Planting in Helvetia Estate, PT.Perkebunan Nusantara II. The purpose of this study was to find out the insect species diversity index inoil palm (Elaeis guineensis Jacq.) planting in Helvetia Estate, PT. Perkebunan Nusantara II. This surveystudy was conducted from June to August 2101 in Helvetia Estate, PT. Perkebunan Nusantara II, DeliSerdang District with the altitude of ± 23 meters above sea level. The insects were caught by busingsweep net, pit fall trap, and light trap, then they were observed at the Plant Pest and Disease Laboratory,Faculty of Agriculture, University of Sumatera Utara. The result of this study showed that 298 insectsconsisting of 9 orders and 29 families were trapped and caught in the oil palm plantation area which hasproduced and 367 insects consisting of 10 orders and 34 families were trapped and caught in the oil palmplantation area which has not produced.The value of Insect Species Diversity Index according to thehighest Shanon-Weiner (H’) in the oil palm plantation area which has produced is 2.9276 (adequate) andin the oil palm plantation area which has not produced is 2.9848 (adequate). While the value ofSorensen’s Quotien of Similarity for the kinds of insects in both areas is 79.365%.Keywords: Diversity index, oil palm, insect

UJI ANTAGONISME Fusarium oxysporum f.sp. passiflora TIPE MUTASIAN TERHADAP Fusarium oxysporum f.sp. passiflora TIPE LIAR DI LABORATORIUM Lisnawita Lisnawita; Muklis Adi Putra; Hasanuddin Hasanuddin; Lisnawita Lisnawita
Jurnal Agroekoteknologi Vol 2, No 1 (2013)
Publisher : Universitas Sumatera Utara

Antagonism test between mutated type of Fusarium oxysporum f.sp passiflora against wild type ofFusarium oxysporum f.sp passiflora in laboratory. This research aims to determine antagonismpotentiality from mutated type of F. oxysporum f.sp. passiflora against wild type of F. oxysporumf.sp. passiflora. The research was conducted at Plant Disease Laboratory, AgroecotechnologyProgram Study, Faculty of Agriculture University of Sumatera Utara, Medan from September toNovember 2012. The research was done by using Completely Randomized Design (CRD) NonFactorial with ten treatments and three replications. The result showed that the highest percentageof inhibiting zones contained in 60 minutes ulta violet (UV) irradiated isolate (M9) at 54.45% andthe lowest were in without UV irradiated isolate (M0) at 39.27%, highest extensive growth ofmycelium from wild F. oxysporum was founded at 60 minutes UV irradiated isolate (M9) at 35.05cm2 and the lowest were 12 minutes UV irradiated (M5) at 25.36 cm2. The highest colony diameterwas founded at 60 minutes UV irradiated isolate (M9) at 5.53 cm and the lowest were 12 minutesUV irradiated (M5) at 4.67 cm.Keywords : Fusarium oxysporum f.sp passiflora, antagonism, UV irradiation

ISOLASI JAMUR Oncobasidium theobromae P.H.B TALBOT & KEANE PENYEBAB PENYAKIT VASCULAR STREAK DIEBACK PADA TANAMAN KAKAO DI LABORATORIUM Nugraha Pratama Dhana; Lahmuddin Lubis; Lisnawita Lisnawita
Jurnal Agroekoteknologi Vol 2, No 1 (2013)
Publisher : Universitas Sumatera Utara

Isolation of fungi Oncobasidium theobromae P.H.B Talbot and Keane cause disease VascularStreak Dieback (VSD) on cocoa plants in the laboratory. The research aims to isolateO. theobromae cause Vascular Streak Dieback (VSD) disease on cocoa in the Laboratory. Theresearch was conducted at the Laboratory of Plant Desease Faculty of Agriculture, University ofSumatra Utara.. The research results showed that O. theobromae has a mycelium shaped like cottonand thick white cream, thin walled hyphae, hyaline, with irregular septa, spores an oval with oneflat side, 10-15μm in length and width of 8-10μm.Keywords: Vascular Streak Dieback, Oncobasidium theobromae, cocoa

POTENSI CENDAWAN ENDOFIT DALAM MENGENDALIKAN Fusarium oxysporum f.sp. cubense DAN NEMATODA Radopholus similis Cobb. PADA TANAMAN PISANG BARANGAN (Musa paradisiaca L.) DI RUMAH KACA Rizki Trianno Sinaga; Lisnawita Lisnawita
Jurnal Agroekoteknologi Vol 2, No 1 (2013)
Publisher : Universitas Sumatera Utara

Potential of endophytic fungal to control Fusarium oxysporum f.sp. cubense (Foc) andnematode Radopholus similis (Rs) on banana cv. Barangan (Musa paradisiaca L.) crop ongreenhouse. This research aims to intens and find potential endophytic as biocontrol agents tocontrol Foc and Rs on banana cv. Barangan crop. This research used Completely RandomizedDesign (CRD) non factorial consist thrithteen treatments and three replications. Replications;control (without inoculated), inoculated 300 Rs, inoculated Foc, inoculated 300 Rs one week laterinoculated Foc two weeks later inoculated endophytic 5BSBH’s isolate, inoculated 300 Rs oneweek later inoculated Foc two weeks later inoculated endophytic 4BSU’s isolate, inoculated 300 Rsone week later inoculated Foc two weeks later inoculated endophytic 1BJH’s isolate, inoculated 300Rs one week later inoculated Foc two weeks later inoculated endophytic 1BTAH’s isolate,inoculated 300 Rs one week later inoculated Foc two weeks later inoculated endophytic 1BSHT’sisolate, inoculated endophytic 5BSBH’s isolate one week later inoculated 300 Rs two weeks laterinoculated Foc, inoculated endophytic 4BSU’s isolate one week later inoculated 300 Rs two weekslater inoculated Foc, inoculated endophytic 1BJH’s isolate one week later inoculated 300 Rs twoweeks later inoculated Foc, inoculated endophytic 5BSBH’s isolate one week later inoculated 300Rs two weeks later inoculated Foc, inoculated endophytic 1BSHT’s isolate one week laterinoculated 300 Rs two weeks later inoculated Foc. The results showed that endophytic fungal hadthe potency as biocontrol agent in controlling Fusarium wilt of banana cv. Barangan.Keywords: Endophytic fungal, Fusarium oxysporum f.sp. cubense, Radopholus similis.

INVENTARISASI JAMUR PENYEBAB PENYAKIT PADA TANAMAN KRISAN (Chrysanthenum morifolium) DI KECAMATAN BERASTAGI, KABUPATEN KARO, SUMATERA UTARA Lisnawita Lisnawita Daniel Erikson Hutabarat1*, Lisnawita2, Lahmuddin Lubis2; Lahmuddin Lubis2; Daniel Erikson Hutabarat
Jurnal Agroekoteknologi Vol 2, No 2 (2014)
Publisher : Universitas Sumatera Utara

Researech on tittle the inventarization fungus which is caused diseases on Chrysanthemummorifolium in Sub-district Berastagi, Regency Karo, North Sumatera. This research was intents toknow and to inventary fungus that caused Chrysanthemum morifolium disesases in field and ingreen house in Sub-district Berastagi, Regency Karo, North Sumatera. This research carried out inLaboratory of Plant Pathology Faculty of Agriculture, University of North Sumatera . The Resultsof this research showed that fungus caused Chrysanthemum morifolium diseases were cultivated infield are Puccinia horiana, Septoria sp., Fusarium sp. on flower, and Fusarium sp. on the stem,whereas Chrysanthemum morifolium were cultivated in green house are Puccinia horiana andSeptoria sp..Keywords : inventarization, fungus, Chrysanthemum morifolium

PENGARUH JENIS INSEKTISIDA TERHADAP LALAT BIBIT (Ophiomyia phaseoli Try.) PADA TANAMAN KEDELAI (Glycine max L.) Yuan Cynthia Br. Simanjuntak; Yuswani Pangestiningsih; Lisnawita Lisnawita
Jurnal Agroekoteknologi Vol 2, No 3 (2014)
Publisher : Universitas Sumatera Utara

Research on title effect of insecticides to control bean fly (Ophiomyia phaseoli Try.) on soybean(Glycine max L.). Using chemical insecticide to control bean fly unwisely caused pest resistance.For that purpose in this research used biology, botany, and chemical insecticide to determine theireffectiveness. This research was conducted at Kelurahan Berngam, Kecamatan Binjai Kota,Kotamadya Binjai from June to July 2013 using factorial randomized block design. The resultshowed used soursop leaf 200 gr/l of water (P1) and Bacillus thuringiensis 1 ml/l of water (P5) wereeffective to control population of bean fly with percentage of attack (0.00%) and number of larvae(0.00 larvae). Using soursop leaf 200 gr/l of water (P1) also effective to control number of bean flywas (0.00 bean fly). Time symtomp in P1 (soursop leaf 200 gram/l of water) also lower than theother treatments was six days after planted.Keywords: insecticide, Glycine max, Ophiomyia phaseoli

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