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All Journal Reaktor Alchemy : Journal of Chemistry BERITA BIOLOGI Jurnal Kimia Riset FaST : Jurnal Sains dan Teknologi Pharmaceutical Sciences and Research (PSR) Makara Journal of Science
Antonius Herry Cahyana
Departemen Kimia, FMIPA, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Energy Environmental Science Materials Science & Nanotechnology

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Journal : BERITA BIOLOGI

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SKRINING DAN ISOLASI METABOLIT AKTIF ANTIBAKTERI KULTUR JAMUR ENDOFIT DARI TUMBUHAN Albertisia papuana Becc. [Screening and Isolation of Antibacterial Active Metabolite from the Culture of Endophytic Fungi from Albertisia papuana Becc.] Fathoni, Ahmad; Ilyas, Muhammad; Cahyana, Antonius Herry; Agusta, Andria
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8087.938 KB) | DOI: 10.14203/beritabiologi.v12i3.639

Abstract

Totally 15 isolates of endophytic fungi were obtained from leaves and young stems of a medicinal plant Albertisia papuana Becc.(Menispermaceae). The antibacterial screening of the ethyl acetate extract derived from the fungi cultures in potato dextrose broth (PDB)were performed on a non eluted Thin Layer Chromatography Bioautography assay (TLC bioautography assay). From the screening test, itwas found that the ethyl acetate extract of the fungus Xylaria sp. DAP-KRI-5 culture showed strong antibacterial activity against Staphylococcus aureus. The isolation of active metabolite based on the bioautography guided assay from ethyl acetate extract of the Xylaria sp. DAP-KRI-5, led us to identify phloroglucinol as a main antibacterial compound. The chemical structure of phloroglucinol was deduced from it spectral data, including UV-Vis, 1H and 13C-NMR, GC-MS and published data elsewhere.
Co-Authors Ahmad Fathoni Aliya Nur Hasanah ANDRIA AGUSTA Ardiansah, Bayu Fikriya, Sakti Hidayati Kurniawan, Felie Virgayani Laurentius Haryanto Laurentius Haryanto, Laurentius Lumantobing, Lukas Hotma Parulian Marissa Angelina Maryana, Rianti Merly, Siti Muhammad Ilyas Putria, Devi Kasih Ratna Handayani Saputra, Ziyan Saputri, Ariyanti Saputri, Yosephine Liliana Intan Danar Sofa Fajriah Yunarti, Rika Tri