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PERBAIKAN GENETIK ANGGREK ALAM Vanda spp MELALUI PERSILANGAN INTERSPESIFIK DALAM MENDUKUNG PERKEMBANGAN ANGGREK DI INDONESIA Hartati, Sri; Sumijati, Sumijati; Pardono, Pardono; Cahyono, Ongko
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 1 (2014): Articles in Press
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Abstract

Upaya peningkatan mutu genetik Anggrek memiliki kendala pada teknik persilangan dan perbanyakan biji hasil persilangan. Penelitian  bertujuan untuk: (1) mendapatkan teknik persilangan yang dapat menghasilkan biji dengan tingkat fertilitas tinggi, (2) untuk mendapatkan  hibrida baru yang memiliki keunggulan karakter pada bunga. Penelitian dilakukan di Pusat Konservasi Tumbuhan Kebon Raya LIPI Bogor .Penelitian merupakan persilangan interspesifik yaitu ♀Vanda celebica x ♂ Vanda tricolor,♀Vanda celebica x ♂Vanda dearei, ♀Vanda celebica x ♂Vanda insignis. Dan persilangan resiprok (kebalikan) yaitu ♀ Vanda tricolor x ♂ Vanda celebica,  ♀ Vanda dearei x  ♂Vanda celebica, ♀ Vanda insignis x ♂Vanda celebicaPersilangan dilakukan dengan cara menyilangkan tetua terpilih sebagai tetua jantan atau betina. Kegiatan meliputi karakterisasi tetua, persilangan, panen, penyebaran biji, Penyebaran biji dan transfer dilakukan secara aseptik di laboratorium kultur jaringan. Pengamatan meliputi keberhasilan persilangan, saat terbentuk buah, masak buah, dan terbentuk protokorm.      Dari penelitian dihasilkan 5 seri silangan sampai terbentuk protokorm yaitu Vanda dearei x Vanda Celebica, Vanda celebica x Vanda dearei, Vanda insignis x Vanda celebica, Vanda celebica x Vanda insignis, Vanda  celebica x Vanda tricolor. Keberhasilan persilangan 33%-100%, Waktu yang diperlukan untuk masaknya biji bervariasi antara 122-262 hari, lama berkecambah  16-23 hari.
STUDI KARAKTERISASI ANGGREK SECARA SITOLOGI DALAM RANGKA PELESTARIAN PLASMA NUTFAH Hartati, Sri; Darsana, Linayanti; Cahyono, Ongko
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 1 (2014): Articles in Press
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Abstract

Karakter sitologi anggrek  alam sangat penting dipelajari guna mendukung keberhasilan pemuliaan anggrek. Tujuan penelitian untuk mempelajari karakter sitologi anggrek ( jumlah, ukuran ) serta karyotipe kromosom dalam rangka pelestarian plasma nutfah. Tempat penelitian analisis sitologi dilakukan di Laboratorium Bioteknologi  Fakultas Pertanian UNS Surakarta. Bahan penelitian adalah bagian ujung akar dari  Paphiophedilum glaucophylum,, Coelogyne spesiosa Dendrobium crumentum, Dendrobium mutabile, , Bulbophyllum. blumei.dan  Bulbophyllum beflorum.  Metode yang digunakan dalam penelitian ini adalah metode squashing (pencet) meliputi: fiksasi : larutan Carnoy 2 (6 etanol : 3 kloroform : 1 asam asetat glasial 45%), hidrolisis (larutan HCl 1 N ) dan pewarnaan kromosom (larutan aceto-orcein 2%). Pengamatan meliputi (jumlah, ukuran, bentuk) kromosom dengan mikroskop cahaya.  Hasil penelitian ini menunjukkan :.1.Jumlah Kromosom Anggrek Paphiopedilum glaucophylum, Coelogyne speciosa, Dendrobium crumenantum, Dendrobium mutabile dan Bulbophyllum blume dan Bulbophyllum beflorum mempunyai jumlah kromosom yang sama yaitu 2n = 38, sedang Ukuran kromosom Paphiopedilum glaucophyllum  4.26 ± 0.14 sampai 9.73± 0.19, Coelogyne spesiosa 0.35 ± 0.03 sampai 6.30 ± 0.84, Dendrobium crumenantum 2.46 ± 0.20 sampai 5.33 ±  0.02 Dendrobium mutabile  2.56 ±  0.72 sampai 6.54±   0.73 , Bulbophyllum blume 2.76 ±  0.09 sampai 6.07 ±  0.43  dan Bulbophyllum beflorum1.04 ±  0.07 sampai 1.35 ±  0.16 . Pola karyotipe Paphiopedillum glaucophyllum 2n = 38 m, Dendrobium crumenantum 2n = 38, Bulbophyllum blumei 2n = 38 m , Bulbophyllum beflorum 2n = 38 m sedang Coelogyne spesiosa 2n = 37 m+ 1 ak dan Dendrobium mutabile 2n = 37 m+ 1 sm
PENINGKATAN RAGAM GENETIK ANGGREK Dendrobium spp MELAUI HIBRIDISASI UNTUK MENDUKUNG PERKEMBANGAN ANGGREK DI INDONESIA Hartati, Sri; Budiyono, Agus; Cahyono, Ongko
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 29, No 2 (2014)
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Abstract

Perbaikan genetik melalui persilangan interspesifik antara tetua terpilih anggrek Dendrobium spp diarahkan untuk meningkatkan mutu genetik dan nilai ekonomi anggrek alam dipasaran. Anggrek Dendrobium adalah salah satu genus anggrek yang banyak diminati. Upaya peningkatan mutu genetik anggrek memiliki kendala pada teknik penyilangan dan perbanyakan biji hasil hibridisasi/persilangan. Penelitian bertujuan untuk: (1) mendapatkan teknik penyilangan yang dapat menghasilkan biji dengan tingkat fertilitas tinggi dan (2) mendapatkan anggrek hibrida baru yang memiliki keunggulan-keunggulan karakter. Penelitian dilakukan di Pusat Konservasi Tumbuhan Kebon Raya LIPI Bogor. Persilangan dilakukan pada 4 sampai 6 individu yang berbunga. Persilangan dilakukan dengan cara menyilangkan tetua terpilih sebagai tetua jantan atau betina Pollinia ditransfer dari anther ke stigma dengan menggunakan tusuk gigi steril, dengan metode (i) crossing , (ii) Resiprocal.Pengamatan dilakukan terhadap karakter induk yang digunakan, waktu persilangan sampai dengan panen buah, dan lama berkecambah. Dari penelitian dihasilkan 4 seri silangan secara resiprok sampai perkecambahan yaitu ♀ Dendrobium mirbelianum x ♂ Dendrobium liniale, ♀ Dendrobium liniale x ♂ Dendrobium mirbelianum, ♀ Dendrobium liniale x Dendrobium bigibbum, ♀ Dendrobium bigibbum x ♂ Dendrobium liniale. Persentase keberhasilan semua persilangan dan resiproknya adalah 100% kecuali persilangan ♀ D.lineale x ♂D. tobaense dan resiproknya tingkat keberhasilannya 0%. Waktu terbentuk buah 3-9 hari, masaknya biji bervariasi antara 81-123 hari dan lama terbentuk protokorm bervariasi 15-36 hari.
PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale Hartati, Sri; Budiyono, Agus; Cahyono, Ongko
Caraka Tani: Journal of Sustainable Agriculture Vol 31, No 1 (2016): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.782 KB) | DOI: 10.20961/carakatani.v31i1.11938

Abstract

Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  hybrids orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of Benzyl Amino Purine /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that the addition  of NAA 3 ppm as much as 4,96 cm  and BAP 3 ppm as much as 4,41 cm  give a significant effect on the increase of high plantlets and  so NAA 3 ppm as much as 5,76 cm  effect  on  roots length , but did not significantly effect the number of leaves and roots.
PENINGKATAN RAGAM GENETIK ANGGREK Dendrobium spp MELAUI HIBRIDISASI UNTUK MENDUKUNG PERKEMBANGAN ANGGREK DI INDONESIA Hartati, Sri; Budiyono, Agus; Cahyono, Ongko
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 2 (2014): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.927 KB) | DOI: 10.20961/carakatani.v29i2.13394

Abstract

Perbaikan genetik melalui persilangan interspesifik antara tetua terpilih anggrek Dendrobium spp diarahkan untuk meningkatkan mutu genetik dan nilai ekonomi anggrek alam dipasaran. Anggrek Dendrobium adalah salah satu genus anggrek yang banyak diminati. Upaya peningkatan mutu genetik anggrek memiliki kendala pada teknik penyilangan dan perbanyakan biji hasil hibridisasi/persilangan. Penelitian bertujuan untuk: (1) mendapatkan teknik penyilangan yang dapat menghasilkan biji dengan tingkat fertilitas tinggi dan (2) mendapatkan anggrek hibrida baru yang memiliki keunggulankeunggulan karakter. Penelitian dilakukan di Pusat Konservasi Tumbuhan Kebon Raya LIPI Bogor. Persilangan dilakukan pada 4 sampai 6 individu yang berbunga. Persilangan dilakukan dengan cara menyilangkan tetua terpilih sebagai tetua jantan atau betina Pollinia ditransfer dari anther ke stigma dengan menggunakan tusuk gigi steril, dengan metode (i) crossing , (ii) Resiprocal.Pengamatan dilakukan terhadap karakter induk yang digunakan, waktu persilangan sampai dengan panen buah, dan lama berkecambah. Dari penelitian dihasilkan 4 seri silangan secara resiprok sampai perkecambahan yaitu ♀ Dendrobium mirbelianum x ♂ Dendrobium liniale, ♀ Dendrobium liniale x ♂ Dendrobium mirbelianum, ♀ Dendrobium liniale x Dendrobium bigibbum, ♀ Dendrobium bigibbum x ♂ Dendrobium liniale. Persentase keberhasilan semua persilangan dan resiproknya adalah 100% kecuali persilangan ♀ D.lineale x ♂D. tobaense dan resiproknya tingkat keberhasilannya 0%. Waktu terbentuk buah 3-9 hari, masaknya biji bervariasi antara 81-123 hari dan lama terbentuk protokorm bervariasi 15- 36 hari.
UJI TINGKAT KOMPATIBILITAS DAN UMUR MEKAR BUNGA PADA PERSILANGAN INTERGENERIK ANGGREK Vanda sp DAN Phalaenopsis sp Hartati, Sri; Cahyono, Ongko; Lestari, Nunik Puji
Caraka Tani: Journal of Sustainable Agriculture Vol 32, No 1 (2017): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.762 KB) | DOI: 10.20961/carakatani.v32i1.15924

Abstract

The study aims to determine the effect of compatibility level to the intergeneric crossing ability and the age of the flower blooming of Vanda sp and Phalaenopsis sp. Research was conducted at a screen house and Plant Breeding Laboratory of Faculty of Agriculture, Sebelas Maret University. Crossing is done by inserting pollen of male parent to the stigma of the female parent which were selected based on a predetermined number of crosses. The observations included: percentage of successful crosses, time of fruit formation, durability of hanging fruit, the percentage of fruit ready for harvest, the percentage of the fallen fruit, and the level of compatibility. The study concluded that the intergeneric cross between Vanda sp and Phalaenopsis sp can be done until the age of 3 weeks after the flowers bloom. The fruit begins to form at the age of 6-12 day after crosses (DAC) and can be harvested after reaching the age of about three months. The intergeneric cross between Vanda sp and Phalaenopsis sp is compatible, because this was capable of producing the fruit of more than 60%.
Effect of Fast Dissolved Phosphorus Fertilizer on the Growth, Seed Product, and Phosphorus Uptake Efficiency of Soybean (Glycine max L.) Cahyono, Ongko; Minardi, Slamet
AGRIVITA, Journal of Agricultural Science Vol 44, No 1 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i1.3002

Abstract

The appropriate method of P fertilizer application is very important for the efficiency of P uptake and yield improvement. This research aimed to assess the growth response, seed product, and P uptake efficiency of soybean to the application of fast dissolved phosphorus fertilizer. Two consecutive potted experiments under greenhouse condition were conducted using Alfisols from Jumantono Karanganyar as planting media. The soybeans were planted and treated with six doses of P fertilizer. The treatments were designed in a completely randomized experiment with 5 replications. In the first experiment, the plants were harvested at the maximum vegetative growth for measurement of plant growth components and P uptake. In the second experiment, the plants were harvested at optimum grain maturity for seed yield and P efficiency measurements. The results showed that the application of fast dissolved-P fertilizer improved plant growth, seed yield and P uptake efficiency. Fast dissolved-P fertilizer was optimum at a dose of 100 kg/ha which was applied in a split application method at 0, 15, and 30 days after planting. However further field research is necessary to confirm the results.
IMPROVEMENT OF PHOSPHATE FERTILIZATION METHOD IN WETLAND RICE Ongko Cahyono; Sri Hartati
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.114

Abstract

The low efficiency of Phosphate fertilization on wetland rice may be overcame by using quickly dissolve P fertilizer. However, for soils containing high dissolve Fe, such as Alfisol and Oxisol, will induce the formation of Fe-P bonding. Therefore, to use the quickly dissolve P fertilizer  in such soils should be done by split application. The research aimed to assess better P fertilization method for wetland rice which used quickly dissolve P fertilizer given in split application. The research concluded that the application of quickly dissolve P fertilizer to Alfisol and Oxisol which was applied in split application by twice (0 and 30 days after planting) and three times (0, 15 and 30 days after planting) increased the eficiency of P fertilization in wetland rice.  The split application of 75 kgs perhectare (one half of recommended dose) in Alfisol yielded dry seed as high as 6,374 kgs per hectare (at the twice application), 6,280 kgs per hectare (the three times application)c while the control treatment yielded as high as 6,027 kgs per hectare.  In Oxisol the yield of rice was 8,200 kgs per hectare fo the control treatment, 8,027 kgs per hectare for the twice application and 8,440 kgs per hectare for the three time applications.
PEMAKAIAN PUPUK ALAM BERBAHAN BAKU ZEOLIT DAN KOMPOS PADA BUDIDAYA TANAMAN CABAI (CAPSICUMFRUTESCENS) ONGKO CAHYONO
JURNAL ILMIAH AGRINECA Vol. 15 No. 1 (2015): JURNAL AGRENECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v15i1.346

Abstract

This study aimed to assess the dose of zeolite plus organic compost and inorganic fertilizer on yield of chili pepper (Capsicum frutescens L). This study was conducted fromApril – July, 2012 in the Laboratory of Soil Science and in the glass house of Faculty of Agriculture UTP Surakarta, and was done under factorial design which was arranged in randomized completely design (CRD) consisting of two factors (doses of zeolite and doses of inorganic fertilizers) and three replications. The study concluded that the use of zeolit+organic compost increased yield of chili pepper significantly.  Application of zeolit+organic compost as much as 500 kgs ha-1 and 1000 kgs ha-1 increased significantly the weigth of fruit at every level of inorganic fertilizer.  By applying one half of dose of inorganic fertilizer, the treatment of 500 kg ha-1 of zeolit+compost yielded chili fruit as high as 141.67 grams per plant; and the treatment of 1000 kg ha-1 of zeolit+compost yielded chili fruit as high as 140 grams per plant. Those yield was not significant to the control treatment, which was as high as 141 grams per plant. Therefore it could be concluded that the application of zeolit+compost under doses of 500 kg ha-1 and 1000 kg ha-1 could reduce the use of inorganic fertilizer up until 50 percent.
STUDI EKSPLORASI DAN KARAKTERISASI ANGGREK ALAM SECARA MORFOLOGI DALAM RANGKA PELESTARIAN PLASMA NUTFAH SRI HARTATI; AGUS BUDIYONO; Ongko Cahyono
JURNAL ILMIAH AGRINECA Vol. 14 No. 1 (2014): JURNAL AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v14i1.347

Abstract

Morphological characters orchid species (natural) is very important to learn in order to support successful breeding orchids. Orchids of this species plays an important role as a parent crosses. The purpose of this study was to determine the morphological characteristics of orchid species and genetic diversity of natural orchids in Indonesia.Pelaksanaan some research done in the Faculty of Agriculture exsitu UTP Surakarta from May 2012 until November 2012 Rangcangan design used was Randomized Complete Block with three replications. Accession number consists of 12 orchid species, accession assumed as treatments and replications assumed as a group. Morphological characterization using a scoring guide Characterization of Ornamental Plants by Orchid. Cluster analysis performed by the program NTSYSpc version 2.02i with UPGMA method SimQual function. Dendrogram based on the similarity of 39% obtained by the two groups, the first group consists of Dendrobium liniale, Calanthe triplicate, crumenatum Dendrobium, Eria javanica, spathoglotis plicata, and Acriopsis javanica. The second group consisted of Cymbidium bicolor, Rhynchontylis retusa blume, Vanda tricolor var Suavis, Vanda tricolor var tri color and Vanda Limbata.