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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biotropika Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Muhaimin Rifa’i
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health

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 1 
Immune Response to Burn Injury: Hyperinflammation and Immunosuppression Septin Mauludiyana; Aryati; Yoes Prijatna Dachlan; Iswinarno Doso Saputro; Muhaimin Rifa’i
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15936

Abstract

Burn is an injury to the skin or other tissue. Mostly, it caused by contacting with hot liquids, solids, and flames. The important thing that should be consider in burn incident is the severity of burn and it based on the depth and area of the burn injury. The severity of burns will cause differences of pathophysiological responses. This was a literature review study. Various articles were collected from online database including reports, journals, and published in the last 10 years. The articles were from the scholar journals. The systemic inflammatory response in severe level of burns was not given good response to disappear of lesion burn and initiating tissue repair. Moreover, it was given an organ failure to the patient. The body responded to this incident by releasing antiinflammatory mediators. This response is very strong and prolonged, so it caused immunosuppression and increase the risk of secondary infection to the patients. Burns affects the patient’s immune system. The ratio between pro and antiinflammatory mediators are determining the patient’s subsequent status.
Analisis Mobilisasi Sel T CD4+ dan CD8+ pada Timus Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Simplisia Polyscias obtusa Swastika Pinca Pinca; Muhammad Sasmito Djati; Muhaimin Rifa’i
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

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Abstract

Penggunaan antibiotik dalam pakan sebagai perlindungan bagi ternak dari penyakit memiliki dampak negatif yakni adanya kandungan residu sehingga diperlukan adanya bahan substitusi antibiotik. Simplisia daun kedondong laut (Polyscias obtusa) diduga mengandung senyawa flavonoid yang diduga dapat berperan sebagai agen imunomodulator dalam sistem imun seluler. Tujuan dari penelitian ini adalah untuk mengetahui peran kandungan simplisia daun Polyscias obtusa dalam pakan ayam sebagai imunomodulator pada ayam yang telah diinfeksi bakteri Salmonella typhimurium, ditinjau dari profil ekspresi CD4+, CD8+ dan B220+ yang dianalisis melalui Flowcytometri. Tahapan penelitian meliputi uji konfirmasi bakteri S.typhimurium, persiapan kultur bakteri dan simplisia daun P. obtusa, infeksi bakteri secara oral 500 µl, pembuatan pakan konversi dengan perlakuan simplisia dosis 1 (0.08%), dosis 2 (0.16%) dan dosis 3 (0.26%), isolasi organ timus dan sel T-limfosit dan analisis flowcytometri. Hasil yang diperoleh menunjukan bahwa jumlah relatif CD4+, CD8+ dan B220+ mengalami peningkatan pada perlakuan pakan pabrik+infeksi bakteri Salmonella dan perlakuan penambahan simplisia dosis 2 (0.16%) dengan hasil yang berbeda nyata berdasarkan waktu pemberian pada jumlah relatif sel CD8+. Hal ini membuktikan bahwa simplisia daun P. obtusa memiliki kemampuan dalam memaksimalkan fungsi sistem imun.
The Control of Human Immunosystem by Using Paeony Root Drug Hideo Tsuboi; Muhaimin Rifa’i; Khaled Hossain; Izumi Nakashima; Haruhiko Suzuki
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Paeoniflorin (PF), isolated from paeony root, has been used as a herbal medicine for more than 1200 years in China, Korea and Japan for its anti-allergic, anti-inflamatory and immunoregulatory effects. In this study, we found that PF induces apoptosis in both murine T-lineage cells and human T-cell leukemia Jurkat cells. This apoptosis was mediated through the reduction of mitochondrial membrane potential, activation of caspase and fragmentation of DNA. Interestingly, PF induced generation of reactive oxygen species (ROS) and a reducing agent, dithiothreitol (DTT), and a ROS scavenger, N-acetyl cysteine (NAC), successfully attenuated the PF-induced apoptosis. Additionally, PF induced the phosphorylation of three mitogen-activated protein (MAP) family kinases, extracellular signal-regulated kinase, c-Jun amino-terminal kinase (JNK) and p38 MAP kinase. Curcumin, an anti-oxidant and JNK inhibitor, inhibited PF-induced apoptosis, suggesting the possible involvement of curcumin-sensitive JNK or other redox-sensitive elements in PF-induced apoptosis. These results partially explain the action mechanism of PF-containing paeony root as a herbal medicine.Keywords: paeoniflorin; paeony root; apoptosis; reactive oxygen species (ROS); redox; CD8+ CD122+ regulatory T cells
Potential Regulatory Functions of Propolis to Ameliorate Hematopoietic Disorder in Diabetic Mouse Model Muhaimin Rifa’i
Journal of Global Pharma Technology Volume 09 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Diabetes mellitus (DM) in autoimmune case, related to cytotoxic T cells activation as a response to autoantigen. In the recent studies, propolis is known as a potential immunomodulator. The aim of this study is to determine the effect of propolis as a regulator to ameliorate diabetic mouse model. In vivo experiment was done using streptozotocin-induced diabetic mice. There are five experiment groups; that are DM group (diabetic mice model without treatment); propolis ethanolic extract treatment group doses of 50, 100, and 200 mg/kg BW; and normal mice group. Effect of propolis was observed 14 days post treatment by flow cytometric analysis. The number of CD8+CD62L-, CD4+CD25+CD62L+(Treg), B220+,  and NK+ cells was assessed from splenic cells. The result showed that administration of propolis for 14 days could reduce the number of cytotoxic T lymphocytes (CTL) memory significantly (p ≤ 0, 05). Especially the doses of 100 and 200 mg/kg BW could suppress the activation of CTL quite similar to those normal groups. Propolis with the dose of 200 mg/kg BW increase the number of  Treg, B220, and NK cells. The increase of Treg may reduce the activation of CTL and prevent tissue damage by autoreactive cells, and finally hematopoietic imbalance can be overcome. Keywords: B220, diabetes mellitus, immunomodulatory, NK, propolis.
Effectiveness of Ethanolic Propolis Extract (EPE) Administration in Ameliorating Diabetic Mice Model Muhaimin Rifa’i
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

Diabetes mellitus (DM) is a group of metabolic disorder characterized by hyperglycemia resulting from defects in insulin action, insulin secretion, or both. Many efforts have been made to overcome this disease but until now has not been fully successful. This study aimed at examining CD34+, TER119+VLA4+, B220+  cell population,  and stromal cell-derived factor 1 (SDF-1) expression in an attempt to correct homeostasis indiabetic mice model with active disease.Three different EPE doses were tested (50, 100, and 200 mg/kg BW).  The oral administration of EPE was conducted for 14 days. On day 15, the bone marrow cells were isolated and then subjected to flow cytometry analysis, and data was processed by One-way ANOVA (p < 0.05) and Tukey test using SPSS 16.0 for Windows. The results showed that administration of EPE with the doses of 50 and 100 mg/kg BW) potentially increased cell proliferation. In contrast, the doses of 200 mg/kg BW seem to be in potentially suppressing cell proliferation. Taken together, propolis, which is involved in the induction of proliferation, show a rather in low doses, while high dose will give a suppression effect on cell proliferation and differentiation. Therefore, the use of propolis for treatment should consider the appropriate dose.  Keywords: B220, CD34, Diabetes mellitus, SDF-1, TER-119+VLA-4+.
Co-Authors Akhmad Fathir Aryati Haruhiko Suzuki Hideo Tsuboi Iswinarno Doso Saputro Izumi Nakashima Khaled Hossain Moch Sasmito Djati Mohammad Hefni Septin Mauludiyana Swastika Pinca Pinca Syamsul Arifin Widodo - Yoes Prijatna Dachlan Yundari, Yundari Zumrotul Mufidah