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All Journal Majalah Kedokteran Bandung Pharmaciana: Jurnal Kefarmasian Biomedical Engineering Majalah Obat Tradisional
Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Antioxidant properties of spice extracts Wahyu Widowati; Hana Ratnawati; Winsa Husin; Maesaroh Maesaroh
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Abstract

Objective : This study was conducted to determine the antioxidant activities of spice extracts including clove (Syzygum aromaticum L.), Indonesian cassia (Cinnamomum burmanni (C. Nees& T. Ness)), coriander (Coriandrum sativum L), nutmeg (Myristica fragrans Houtt), java cardamom (Amomum compactum Soland. Ex maton). Methods : This research was to evaluate antioxidant activities including 1,1-diphenyl-2picryl-hydrazyl (DPPH) scavenging activity, the value of superoxide dismutase (SOD), and total phenolic content. Results : The highest DPPH activity is clove and Indonesian cassia extracts with IC value 4.16 μg/ml and 5.46 μg/ml respectively. The highest SOD value are Indonesian cassia extract (9.1432 U/ml) at 500 µg/ml, 7.0045 U/ml at 125 µg/ml and 4.6751 U/ml at 31,25 µg/ml. Clove extract was the highest of phenolic content  (188.35 µg/mg eugenol equivalent). Conclusion : Indonesian cassia extract have high antioxidant activities. Clove extract contain the highest eugenol compared with Indonesian cassia, coriander, nutmeg and java cardamom.  Key Words: antioxidant; free radicals; spice; total phenolic content
Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fractions Fanny Rahardja; Rita Tjokropranoto; Wahyu Widowati; Siska Lusiana; Adrian Suhendra; Susy Tjahjani; Iwan Budiman; Maesaroh Maesaroh; Nurul Fauziah
Biomedical Engineering Vol 1, No 1 (2014)
Publisher : Biomedical Engineering

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Abstract

Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods: The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction ofgreen tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC. Results: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 µg/ml. The anti-malarial activity IC50 50 of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 µg/mL,0,003 µg/mL, 0,006 µg/mL, 0,011 µg/ml respectively.Conclusion: Extract and fractions of green tea have potential anti-malarial and antioxidant properties. Key Words: antioxidant; anti-malarial; green tea; free radicals
Antioxidant and antiaging activity of rutin and caffeic acid Ermi Girsang; I Nyoman Ehrich Lister; Chrismis Novalinda Ginting; Ika Adhani Sholihah; Martinus Ahmad Raif; Sidharta Kunardi; Hendy Million; Wahyu Widowati
Pharmaciana Vol 10, No 2 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (642.733 KB) | DOI: 10.12928/pharmaciana.v10i2.13010

Abstract

Aging is a complicated process occurring due to the combination of incremental alterations of the skin and accumulated extrinsic factors that causes both structural and functional disruptions. The extrinsic factor of skin aging is mostly caused by free radicals, UV exposures, and pollution. Prevention is possible by escalating antioxidant intake to scavenge ROS in the skin aging process. Rutin and caffeic acid are recognized for their free radical trapping effects and reported to have potential antiaging activities. This study aimed to identify the potentials of rutin and caffeic acid as antioxidant and antiaging. Rutin and caffeic acid were tested for their antioxidant properties using the DPPH, H2O2, ABTS radical scavenging, and FRAP assays. Meanwhile, their antiaging activities were examined by collagenase, elastase, hyaluronidase, and tyrosinase inhibitory assays. The study drew on the evidence of antioxidant and antiaging properties from the scavenging, ferric ion reducing, and inhibitory activities of rutin and caffeic acid (in ascending order): in scavenging DPPH free radicals (IC50 of rutin = 5.79 µg/mL, IC50 of caffeic acid = 8.72 µg/mL), scavenging H2O2 ( IC50 rutin = 12.09 µg/ml, IC50 caffeic acid = 15.23 µg/mL), reducing ABTS (IC50 caffeic acid = 6.23 µg/mL, IC50 rutin = 16.59 µg/mL), reducing ferric ions at 50 µg/mL (FRAP of rutin = 480.08 µM Fe(II)/µg, FRAP of caffeic acid= 526.50 µM Fe(II)/µg), inhibiting collagenase (IC50 caffeic acid = 74.42 µg/mL, IC50 rutin = 104.70 µg/mL), inhibiting elastase (IC50 rutin = 46.88 µg/mL, IC50 caffeic acid = 76.95 µg/mL), inhibiting tyrosinase (IC50 rutin = 55.65 µg/mL, IC50 caffeic acid = 145.91 µg/mL), and inhibiting hyaluronidase (IC50 rutin = 114.07 µg/mL, IC50 caffeic acid= 244.45 µg/mL). Rutin and caffeic acid have the potentials as antiaging and antioxidant.
Potency of Black Soybean (Glycine max (L.) Merr) Extract and Daidzein as Antioxidant and Antihyaluronidase Tandiah Asan; I Nyoman Ehrich Lister; Edy Fachrial; Annisa Amalia; Wahyu Widowati; Buter Samin; Liena Liena
Majalah Obat Tradisional Vol 24, No 1 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.018 KB) | DOI: 10.22146/mot.43615

Abstract

Black soybean (Glycine max (L.) Merr.) is a plant that is widely planted and consumed in Indonesia. In addition, black soybean has unique content of isoflavones, such as daidzein, which is one of the active compounds that have the effect of fighting free radicals and can inhibit the aging process. The purpose of this study is to analyze the antioxidant potency possessed by black soybean extract (BSE) and daidzeinin inhibiting aging of the skin. The method used is a colorimetric test. The type of antioxidant test used is H2O2 scavenging and inhibiting the activity of the hyaluronidase enzyme for antiaging. BSE has better effectiveness of H2O2 scavenging (IC50: 286.24±11.16 (µg/mL)) than daidzein compound (IC50: 366.16±2.54 (µg/mL)). In the inhibition of hyaluronidase enzyme, the daidzein has more effective activities (IC50: 95.80±3.98 (µg/mL)) compared to BSE (IC50: 152.56±13.98 (µg/mL)). The antioxidant and anti-aging activities possessed by BSE make it possible to be used as a cosmetic ingredient for skin aging therapy.
Comparison of Antioxidant and Antiaging Activities Between Dragon Fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose) Rind Extract and Kaempferol Lisma Viany; Rizal Rizal; Wahyu Widowati; Buter Samin; Rita Kusuma; Edy Fachrial; Lister Ehrich I Nyoman
Majalah Kedokteran Bandung Vol 51, No 3 (2019)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v51n3.1715

Abstract

Aging involves progressive physiological changes of the body, including skin dysfunction and death which can be accelerated by the presence of free radicals. Compounds with potential antioxidants and antiaging are contained in various fruits, including dragon fruit (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose). This study aimed to compare the antioxidant and antiaging activities of dragon fruit rind extract (DFRE) and Kaempferol. The antioxidant and antiaging activities were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenger and inhibitory activity assay of tyrosinase, respectively and was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama, from August to November 2018. Data acquired were then analyzed using ANOVA, followed by Post Hoc Test using Turkey HSD test with a confidence level of 95% (α=0.05). Phytochemical assay was also conducted to determine the content of active ingredients in thedragon fruit rind extract. Phytochemical test result showed that DFRE contained flavonoids, tannins, phenols, triterpenoids, and alkaloids compounds. Both DPPH scavenging and tyrosine inhibition assay indicated that Kaempferol compound also had antioxidant and antiaging activities. The IC50 values of DFRE and Kaempferol antioxidant activities were 164.98 μg/mL and 83.30 μg/mL, respectively, while the IC50 values of tyrosinase inhibition activity of DFRE and Kaemferol were 88.46 μg/mL and 59.34 μg/mL, respectively. DFRE had antioxidant activities and tyrosinase inhibition activities thus can be used as an alternative antiaging and antioxidant agents. Perbandingan Aktivitas Antioksidan dengan Antitirosinase Ekstrak Etanol Kulit Buah Naga (Hyloceureus polyrhizus (F.A.C. Weber) Britton & Rose)Aging atau penuaan adalah proses kompleks yang ditandai dengan penurunan progresif fungsi fisiologis tubuh, termasuk kulit yang diikuti oleh disfungsi dan kematian kulit. Faktor-faktor yang dapat mempercepat proses penuaan dini di antaranya radikal bebas dan gaya hidup yang tidak sehat. Buah-buahan banyak mengandung senyawa yang berpotensi sebagai antioksidan dan antiaging, salah satu di antaranya adalah buah naga. Penelitian ini bertujuan mengetahui potensi antioksidan dan antiaging ekstrak kulit buah naga (EKBN) yang dibandingkan dengan senyawa kaempferol (SK) sebagai pembanding. Penelitian yang dilakukan meliputi uji fitokimia untuk mengetahui kandungan bahan aktif pada kulit buah naga, uji pemerangkapan DPPH, dan uji penghambatan tirosinase dan dilakukan di Pusat Penelitian Biomolekul dan Biomedis, Aretha Medika Utama, dari Agustus hingga November 2018. Data lalu dianalisis menggunakan analisis varians (ANOVA) satu arah (one way) Hasil penelitian uji fitokimia menunjukkan EKBN mengandung golongan senyawa, flavonoid, tanin, fenol, triterpenoid, dan alkaloid serta tidak mengandung golongan senyawa saponin, steroid, dan terpenoid. Senyawa kaempferol memiliki aktivitas antioksidan melalui pemerangkapan DPPH dan aktivitas antiaging melalui penghambatan tirosinase yang lebih baik dibanding dengan EKBN. Aktivitas antioksidan dilihat dari nilai IC50 EKBN sebesar 164.98 μg/ml dan SK sebesar 83.30 μg/mL. Aktivitas penghambatan tirosinase memiliki nilai IC50 EKBN masing-masing sebesar 88.46 μg/mL dan IC50 SK sebesar 59.34 μg/mL. EKBN memiliki aktivitas antioksidan dan penghambatan tirosinase sehigga dapat dimanfaatkan sebagai alternatif bahan antiaging dan antioksidan. 
Co-Authors Adek Armansyah Adrian Suhendra Annisa Amalia Buter Samin Buter Samin Chrismis Novalinda Ginting Chrismis Novalinda Ginting Edy Fachrial Ermi Girsang Ermi Girsang Fanny Rahardja Geeta Geeta HANA RATNAWATI Hendy Million I Nyoman Ehrich Lister I Nyoman Ehrich Lister I Nyoman Ehrich Lister Ika Adhani Sholihah Iwan Budiman Liena Liena Lisma Viany Lister Ehrich I Nyoman Maesaroh Maesaroh Maesaroh Maesaroh Martinus Ahmad Raif Nurul Fauziah Rita Kusuma Rita Tjokropranoto Rizal Rizal Sidharta Kunardi Siska Lusiana Susy Tjahjani Tandiah Asan Wahyu Setia Widodo Winsa Husin