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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Jurnal Ilmu dan Teknologi Kayu Tropis Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Journal of Innovation Research and Knowledge Jurnal Riset Rumpun Ilmu Tanaman Bionature
Joko Sulistyo
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Social Sciences

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Journal : Bionature

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Characteristic of Oyster Mushroom Grown on Substrates Containing Paper Waste Joko Sulistyo; Soh Chin Quan; Lee Khaa Hwee
Bionature Vol. 24 No. 2 (2023): Oktober 2023
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

The aim of study was to determine the capacity of spawn of oyster mushroom and influence of substrates containing PW used to grow the mushroom and its properties related to nutritional value and antioxidant activity due to the use of paper waste (PW). The substrate was prepared based on 5 different formulations which were 0% (control), 20%, 30%, 40%, 50% PW. Preparation of seeds and spawn and mushroom cultivation technique was carried out manually using basic techniques of mushroom seeding and cultivation, while proximate analysis was carried out to analyze the content of fat, protein, moisture, and ash of the mushroom fruiting bodies, followed by analysis of heavy metals using AAS, and furthermore carried out extraction of bioactive compounds to analyze antioxidant activity using ABTS oxidizer. The physicochemical analysis was carried out to test the quality of the mushroom. Overall, the mushroom grown on the substrates containing 50% PW showed higher content of ash, protein, fat and crude fiber compared to the mushroom grown on other substrates with different composition of PW. However, the mushroom grown on the substrate contained 50% PW indicated considerably high content of heavy metals such as lead, (3.5%), copper (20.8%) and cadmium (4.4%), respectively, although, it showed the higher antioxidant activity (87.19%) as well compared to the mushrooms grown on the substrate containing a lower percentage of PW, thus giving a conclusion that there was probably positive correlation in between the heavy metals contained in PW with antioxidant activity.
Bioproses Penguraian Limbah Cair Buangan Industri Pangan dan Non-Pangan Berbasis Biosurfaktan Yati Sudaryati Soeka; Joko Sulistyo
Bionature Vol. 23 No. 2 (2022): Oktober 2022
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Biosurfactants are microbial metabolites that may be utilized to replace synthetic chemical surfactant inovercoming the problem of industrial wastewater pollution. Indications of the presence of biosurfactant activitymay be determined through measurement of emulsification and enzymatic activity of the tested microorganismson their growth media, which is indicated by decreasing the BOD and COD of the tested growth media containingwastewater material as an inducer added to the microbial growth medium. This research was conducted toexamine several potential microbial isolates that produce biosurfactants on growth media containing mixed wastewater. The results showed that some of the microbial cultures tested showed the potential to produce biosurfactantsas indicated by the emulsification and enzymatic activity of the tested microbial culture. Trial on mixed industrialwastewater showed that the tested biosurfactants were able to reduce the levels of BOD (440-1300 mg/l) and COD(1240-1760 mg/l) of the wastewater samples up to low BOD levels (82 mg/l) and COD (116 mg/l), respectively,thus that it meets the requirements of the established wastewater quality standards according to the IndonesianStandard regarding with the respective BOD (50-100 mg/l, 06-6989.9-2004 SNI) and COD (100-250 mg /l, 06-6989.2-2004 SNI).
Co-Authors Dudik Djaja Sidarta Evi Triana Fanny Hidayati Ganis Lukmandaru Harry Praptoyo Jennifer Callysta Thee Lee Khaa Hwee Nur Handayati Nyoman Puspa Asri Purnama Dewi Purnomo Darmaji Rini Pujiarti Rostiati NR Napitupulu Soh Chin Quan Sri Nugroho Marsoem Subekti Subekti Tomy Listyanto Toshimitsu Hata Wahyu Prawesthi Yati Sudaryati Soeka Yuji Imamura