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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Jurnal Ilmu dan Teknologi Kayu Tropis Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Journal of Innovation Research and Knowledge Jurnal Riset Rumpun Ilmu Tanaman Bionature
Joko Sulistyo
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Social Sciences

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Journal : BERITA BIOLOGI

 1 
ANALISA ASAM ORGANIK DAN ASAM AMINO PADA NATTO YANG DIFERMENTASI OLEH 7 STRAIN BACILLUS NATTO JOKO SULISTYO
BERITA BIOLOGI Vol 3, No 6 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i6.1326

Abstract

JOKO SUUSTYO. 1986. Analysis of organic acid and amino acid in natto fermented by 7 strains of Bacillus natto. Berita Biologi 3 (6): 277 - 282. The ability of seven strains of Bacillus natto produced a number of organic and amino acid components in natto were investigated. Raw, submerced and autoclaved soybeans for natto contained 1.15% 0.46% and 0.77% in organic acid and 0.19%, 0.30% and 0.30% in amino acid, while after fermentation those range were 0.36 - 0.90% in organic acid and 2.86 -6.08% in amino add. During submercing, autoclaving and fermenting, citric acid component decreased down very sharply to about one hundredth of starting level. While acetic,iso-butiric and iso-valeric components increased up to 84.92%, 96.60% and 95.87%, respectively. Mean while, glutamic acid, iso-leucine,leucine andphenylalanine components of amino acid increased up to 84.36%, 90.52%, 91.67% and 95.88%, respecti
PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) Joko Sulistyo; Yati Sudaryati Soeka; Evi Triana; Rostiati NR Napitupulu
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1246

Abstract

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28°C-40°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 C°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.
SEMI PURIFIKASI DAN UJI REAKSI TRANSGLUKOSILASI P-GLUKOSIDASE DARI Aspergillus pulverulentus Joko Sulistyo; Yati Sudaryati Soeka; Purnama Dewi
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i4.1263

Abstract

An extracellular p-glucosidase[EC 3.2.1.21]derived from Asvereillus vulverulentus were separated and partially purified by successive chromatographies and its some characterization and transglucosylation capacity were studied.The purification protocol included precipitation with ammonium sulphate,gel filtration,ion exchange chromatography and native polyacrylamide gel electrophoresis.The enzyme readily hydrolysed cellobiose to form transglucosylation products in the present of primary alcohol acceptors.This P-glucosidase was stable at temperatures up to 70 °C and from pH 2.5 to 8.5, while its highest activity was in the pH 4.5 at 65°C.
Co-Authors Dudik Djaja Sidarta Evi Triana Fanny Hidayati Ganis Lukmandaru Harry Praptoyo Jennifer Callysta Thee Lee Khaa Hwee Nur Handayati Nyoman Puspa Asri Purnama Dewi Purnomo Darmaji Rini Pujiarti Rostiati NR Napitupulu Soh Chin Quan Sri Nugroho Marsoem Subekti Subekti Tomy Listyanto Toshimitsu Hata Wahyu Prawesthi Yati Sudaryati Soeka Yuji Imamura