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All Journal Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan Jurnal Labora Medika Husada Mahakam: Jurnal Kesehatan Jaringan Laboratorium Medis Tropical Health and Medical Research Journal Jurnal Karya Generasi Sehat

Ratih Dewi Dwiyanti

Department Of Medical Laboratory Technology Poltekkes Kemenkes Banjarmasin

Author-ID : 2504739

Religion Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp Ratih Dewi Dwiyanti; Rion Dediq; Anny Thuraidah
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

edes sp is known as a mosquito that transmits various diseases. In addition to spreading dengue fever and chikungunya, these mosquitoes also spread the virus zika is quite dangerous. One of the effort to controlling these diseases is to eradicate the vector by using materials that can be taken from plants one of them is bay leaf (Syzygium polyanthum). The purpose of this study is to determine the kills power of Syzygium polyanthum water extract against Aedes sp larvae. This research uses true experiment with Posttest Only With Control Group Design design. Data were obtained from the number of larval deaths in the Syzygium polyanthum extract in each control group and treatment group for 24 hours. The results showed that the percentage of bayleaf water extract which has the largest number of larval deaths was at 32% concentration with 55% death percentage. It was concluded that each concentration of aqueous bay leaf water extract given had a killing power against Aedes sp larvae with the lowest percentage of 2.5% and the highest 55%. Based on the result of Kruskal-Wallis test showed that water extract of the bay leaf was able to kill Aedes sp larvae at each treatment with the value of sig 0,001. Suggestion for the next researcher to test the power of killing the water extract of Syzygium polyanthum to Aedes sp larvae by increasing the concentration of salted bay water extract.

Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.

MRSA dan VRSA pada Paramedis RSUD Ratu Zalecha Martapura Ratih Dewi Dwiyanti; Akhmad Muhlisin; Akhmad Muntaha
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstact: Staphylococcus aureus strains isolated from hospital generally been resistant to many antimicrobial-resistant strains of Staphylococcus aureus to vancomycin was rarely reported. Strains of Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections that are multiresistant to antibiotics. This study aims to determine the presence of bacteria Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) on paramedics in the treatment room and the surgical ICU care hospitals Zalecha Martapura queen. This study used a descriptive survey method. The population in this study were nurses who worked in the surgical and ICU care that numbered 38 people. Sampling technique accidental sampling with a sample of 27 people. The survey results revealed 27 positive samples of Staphylococcus aureus 14 people, there is a sensitivity test results of 7 samples were resistant to cefoxitin (MRSA 26%), 4 samples were resistant to oxacilin and 7 samples were resistant to vancomycin (VRSA 26%). Concluded there is MRSA and VRSA in hospital Queen zalecha Martapura, it is advisable to increase the cleanliness of individuals and the use of personal protective equipment to reduce the incidence of nosocomial infections in the scope of the hospital.

Improved Mycology Media Formula of Corn and Cow’s Hair For Accelerate Superficial Mycosis Growth Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Potato Dextrose Agar is growth and identification media of fungi that cause superficial mycoses, including Trichophyton rubrum and Trichophyton mentagrophytes with 10–14 days growth period. Modification media is needed to accelerate the growth of the fungus. Zea mays saccharata has a high carbohydrate, and cow’s hair contains high keratin, which can be used as a nutrient to replace fungal growth media. The study aimed to obtain a modified media formula containing Zea mays saccharata as a substitute for dextrose and cow's hair as a source of keratin to accelerate the growth of Trichophyton rubrum and Trichophyton mentagrophytes fungi. The modified media formula is mixing of Zea mays saccharata 15% to 25%, and cow's hair 20mg to 35mg. Acceleration of growth time based of when found microconidia and macroconidia, diameter measurements, and observations of texture and colonies color of Trichophyton rubrum and Trichophyton mentagrophytes. The study results are Zea mays saccharata media with the addition of cow hair for Trichophyton rubrum grew 11 days with 10%+20mg formula and grew only seven days with 25%+35mg formula. Modified media for Trichophyton mentagrophytes grew seven days for 10%+20mg formula, while it was only three days with 25%+35mg formula. The media formula with the fastest growth rate for Trichophyton rubrum and Trichophyton mentagrophytes was at a concentration of 25%+35mg. The Advanced Modified Media can become a patent medium for faster growth of superficial fungi at a lower cost. Further research needs to be done on how long this media can be stored for long-term use.

Daya Hambat NaCl terhadap Pertumbuhan Staphylococcus aureus Amalia Amalia; Ratih Dewi Dwiyanti; Haitami Haitami
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Staphylococcus aureus is a pathogenic bacterial that causes infectious disease and human poisoning through enterotoxin produced by the bacteria. Salt or sodium chloride is used by human for food preservation process because it can inhibit the growth of bacteria, especially Staphylococcus aureus. The purposes of the study were to determine the effect of NaCl in media on the growth of Staphylococcus aureus and determine the effective concentration of NaCl. The type of research used in this study was true experiment posttest only control group design. Samples were prepared in 5 NaCl concentrations, they were 10%, 15%, 20%, 25% dan 30%. The study conclusion was there were effects of NaCl addition to the media on the growth of Staphylococcus aureus with a significance value of 0.000 (

Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi Ratih Dewi Dwiyanti; Nurlailah Nurlailah; Indah Kurnia Widiningsih
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi.

Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli Ratih Dewi Dwiyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.

Co-Authors Agnina Listya Anggraini Ahmad Muhlisin Aima Insana Aima Insana Aima Insana Aina Nurvita Sari Akhmad Muhlisin akhmad muntaha Amalia Amalia Annisa Nur Yulianti Anny Thuraidah Anny Thuraidah Ayu fahani Ayu Sutiany Deby Arnisya Putri Dini Aulia Azmi Dinna Rakhmina Dinna Rakhmina Ekrima Dayanti Erfan Roebiakto Erpan Roebiakto Fransiskus Xaverius Agung Pinto Laksono Haitami Haitami hana nailah Haris Nurdin Pratama Indah Kurnia Widiningsih leka lutpiatina, leka Neni Oktiyani neni oktiyani Nur Asy Syifa Azizah Nur Lailah Lailah Nur Rizka Adriana Nur Rizqi Amaliah Nurlailah Nurlailah Nurlailah Nurlailah Nurmalasari Nurmalasari Nurul Huda Rahmayunita Rahmayunita Rahmayunita Rifqoh Rifqoh Rima Putri Wulandari Rion Dediq Rizky Arsyanti Siti Mas'odah Utama, Ricko Dharmadi wahdah norsiah Yuli Susanti

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