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All Journal Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Jurnal Pengabdian UntukMu NegeRI IJOEP : International Journal of Ecophysiology Dinamika Pertanian
Dewi Indriyani Roslim
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Social Sciences

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POLONG PALING SEDIKIT PADA GALUR KACANG HIJAU (Vigna radiata (L.) Wilczek) LOKAL KAMPAR Desnilia '; Herman '; Dewi Indriyani Roslim
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

The mungbean demand in the market increases but it is difficult to provide the stock because mungbean production is low due to low interest of local people to cultivate thisspecies. Riau Province has common mungbean strain, i.e. Kampar local strain that has low fruit production, unsimultaneous ripe physiological characters, and trichomes. One of the effort of plant breeding use of strain purification. The purpose of this study was to identify the second generation of local mungbean strain from Kampar that has thelowest number of pods. The experiment was conducted from April to June 2013 in the gardens of Biology, Faculty of Math and Natural Science, University of Riau using a completely randomized design. Eight strains were examined with five replications in each strain. The observed character was number of pod. Data was analyzed using SPSS 16.0. The results showed that mungbeans strains (G1, G2, G3, G4, and G6) had thelowest number of pods.
ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando; Roza Elvyra; Dewi Indriyani Roslim
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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JOM FMIPA Volume 1 No. 2 Oktober 2014 224 ISOLASI DAN AMPLIFIKASI WILAYAH mtDNA D-LOOP PADA IKAN Kryptopterus limpok (Bleeker, 1852) DARI SUNGAI KAMPAR KIRI PROVINSI RIAU Rizka Suci Akmando, Roza Elvyra, Dewi Indriyani RoslimMahasiswa Program S1 Biologi Dosen Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Kampus Bina Widya Pekanbaru, 28293, Indonesia rizkasuciakmando@yahoo.co.idABSTRACT Scientific information of mtDNA D-loop region in fish, Kryptopterus limpok, from Riau is unknown. Before having genetic information, total DNA isolation and amplification is necessary to be carried out. This study was aimed to obtain the total DNA molecules and DNA fragment of the mtDNA D-loop region in fish K. limpok from Sungai Kampar Kiri, Riau. Ten muscle samples were taken from fish collected from the river and used for total DNA isolation. Products of DNA isolation were amplified using the PCR technique with universal primers i.e. L15926 (F) 5’ TCA AAG CTT ACA CCA GTC TTG TAA ACC 3’ dan H00651 (R) 5’ TAA CTG CAG AAG GCT AGG ACC AAA CCT 3’. The PCR process consisted of pre-PCR at 940C for 5 minutes, 35 cycles of PCR consisted of three stages, i.e. as denaturation at 940C for 30 seconds, annealing at 590C for 1 minutes, elongation at 720C for 1 minutes, and post-PCR at 720C for 5 minutes. This study obtained the intact total DNA molecules, with the total DNA concentration of 100 μg/ μl. The length of mtDNA D-loop fragment was 153 bp.
cDNA Actin Isolated From Pandanus Sp. Dewi Indriyani Roslim
International Journal of Ecophysiology Vol. 1 No. 2 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

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Abstract

Actin is one of the reference genes that is often used as an internal control in gene expression analysis. This study aimed to isolate actin cDNA from Pandanus sp originated from Riau. Fresh leaves Pandanus sp. Lake Kajuik, Langgam District, Pelalawan Regency, Riau Province. Isolation of RNA, synthesis of total cDNA, amplification of actin genes used McDowell's designed degenerate primer (PlAc46S-20/PlAc245N-20), electrophoresis, sequencing, and data analysis. Actin cDNA fragments obtained were 353 pb in size, registered at GenBank and encoded 117 amino acids. Actin cDNA fragment consists of two exons and one introne. Specific actin primers from Riau Pandanus sp. can be designed based on sequences obtained for the purpose of analyzing certain gene expressions.
PEMURNIAN GALUR KACANG HIJAU (Vigna radiata L.) LOKAL KAMPAR GENERASI KEEMPAT Herman Herman; Misriyani Misriyani; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 32 No. 1 (2016): Jurnal Dinamika Pertanian Edisi April 2016
Publisher : UIR Press

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Kampar Regency is one of the areas of mungbean production in Riau Province. As a germ-plasmin genetic diversity, Kampar mungbean is a local variety that can produce high yield mungbean cultivar. The color of pods and seeds of mungbean may affect the consumer taste. The research was aimed to give information about genetic diversity of Kampar local mungbean and its use as a germ-plasm resource to produce a new cultivar with high yield trait. The research was conducted from August to November 2015 at the Biology garden, Faculty of Mathematics and Science, University of Riau. The randomized complete design with 9 lines and 5 replications was used. The result showed the G0, G1, G2, G5, G6, G7, and G8 lines produced black color pods, while G3 line produced blackish brown, and G4 line produced brown color of pods. The seed color of all of the lines exept G0 produced shiny green, while G0 line produce dull and shiny brownish green seed. The color characteristics of pods and seeds had an effect on the consumer taste, but it did not affect on the yield production. Therefore, this information could be used as germ-plasm resource to produce a new high yield cultivar.
ANALISIS SEBAGIAN SEKUEN DNA DARI GEN MEISA1 PADA UBI KAYU (Manihot esculenta Crantz.) GENOTIPE MENGGALO DAN ROTI Dewi Indriyani Roslim; Shinta Oktavia
DINAMIKA PERTANIAN Vol. 30 No. 2 (2015): Jurnal Dinamika Pertanian Edisi Agustus 2015
Publisher : UIR Press

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Starch metabolism involves isoamylase1 enzyme and the gene encoding of this enzyme in cassava (Manihot esculenta Crantz.) is called meisa1 gene. The study aimed to analyze partial DNA sequences of meisa1 gene on menggalo (as bitter genotype) and roti (as sweet genotype) cassavas. Cassavas were grown in the garden at Department of Biology, Riau University in March to June 2014 with a code MG1, MG2, and MG3 for menggalo and Ry1, Ry2, Ry3 for bread. Research methods included DNA extraction, electrophoresis, PCR, sequencing, and data analysis. Data were analyzed using BLAST (Basic Local Alignment Search Tools) and MEGA (Molecular Evolutionary Genetics Analysis) program version 6:06. The results showed that there were few substitution mutations in the partial DNA sequence of meisa1 gene on cassava menggalo and roti cassavas and those mutation occured frequently in intron regions. Menggalo and roti cassavas had more Adenin (A), i.e. 34.7% and 35.2%, respectively. The ratio of (A + T) / (G + C) for the sixth cassava plants is almost the same ranging from 1.67 to 1.78. Genetic distance between the two genotypes of cassava based partial DNA sequences of meisa1 gene ranged from 0.11 to 0.21.
PENGARUH DOSIS KOMPOS PELEPAH KELAPA SAWIT TERHADAP HASIL UMBI PADA LIMA GENOTIPE UBI KAYU (Manihot esculenta Crantz.) Indah Mulyasari; Herman Herman; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 30 No. 1 (2015): Jurnal Dinamika Pertanian Edisi April 2015
Publisher : UIR Press

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In order to get the optimum yield, fertilization should be based on plant need of nutrient. The aim of this research was to find out the optimum dosage of oil palm stem compost for tuber yield of five cassava genotypes. The research was conducted at the biology experimental farm of Riau University, Pekanbaru situated at above ± 5 meters elevation during eleven months from July 2013 to May 2014. The research was arranged in the Randomized Block Design with two factors. The first factor was five cassava genotypes namely: Emas cassava (IM1), Lambau cassava (IM2), Menggalo cassava (IM3), Pulut cassava (IM4), Grafting cassava (IM5). The second factor was dosage of oil palm stem compost with three levels namely: 0 kg (P0), 5 kg (P5), 7 kg (P7). The parameters observed were plant height, number of tubers/plant, tuber diameter, tuber length, and tuber weight/plant. The results showed that there was significantly interaction between doses of oil palm stem compost and cassava genotypes on the tuber weight/plant but not significantly on plant height, number of tubers/plant, length and diameter of tuber. The compost with dosage of 5 kg was optimum for Lambau and Pulut cassavas, while the compost with dosage of 7 kg was optimum for Emas, Menggalo, and Grafting cassavas.
GENOTIPE PADI LOKAL ASAL BENGKALIS PROVINSI RIAU YANG TOLERAN CEKAMAN ALUMINIUM Endah Budi Lestari; Dewi Indriyani Roslim; Herman Herman
DINAMIKA PERTANIAN Vol. 29 No. 2 (2014): Jurnal Dinamika Pertanian Edisi Agustus 2014
Publisher : UIR Press

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The major problem in acid soils is aluminium toxicity that can harm the rice plant roots. The objective of this study is to identify of Al-tolerant local rice genotypes from Bengkalis, Riau Province. Plant materials tested were four local rice genotypes from Desa Bantan Air, Kecamatan Bantan, Kabupaten Bengkalis, Riau Province, i.e., Amat Candu, Sadani, Solok, dan Yamin. Grogol variety was used as an Al-tolerant rice variety, while IR64 variety was used as an Al-sensitive rice variety. All genotypes/varieties were exposed to 12 ppm Al for 72 hours in nutrient culture, pH 4. Each treatment was repeated three times. Ten seedlings per genotype/variety were tested. Randomized Block Design was used in this research. The results showed that Yamin had the smallest PPA value (67%), followed by Grogol and Sadani (70%), Amat Candu (71%), Solok (72%), and IR64 (80%). Grogol and Sadani had same value of PPA and PAR, which demonstrated that both rice genotypes were tolerance to Al stress. It concluded that there were two Al-tolerant local rice genotypes from Bengkalis, such as Sadani and Yamin, while two others were Al-moderate, such as Amat Candu and Solok.
ANALISIS SEKUEN INTRON 1 SAMPAI SEBAGIAN EKSON 4 DARI GEN FERITIN2 PADA TIGA GENOTIPE PADI (Oryza sativa L.) LOKAL INDRAGIRI HILIR, RIAU Herman Herman; Yolla Putri Ardila; Fadel Nugraha; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 29 No. 1 (2014): Jurnal Dinamika Pertanian Edisi April 2014
Publisher : UIR Press

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Iron toxicity is one of the limiting factors of rice crop production in lowland area. Iron homeostasis in cell involves the role of ferritin protein. The objective of this study is to analyze potion of first intron to fourth exon sequence of local rice varieties from Indragiri Hilir, Riau Province. Plant material were three local rice genotype, namely Bakung, Siputih, and Serei, and two rice varieties consisted of IR64 (Fe toxicity-sensitive rice variety) and Siam Sintanur (Fe toxicity-tolerant rice variety). Methods include DNA isolation, PCR (Polymerase Chain Reaction), electrophoreses, and sequencing. The DNA sequence was analyzed using BLASTn and ClustalW2 programs. This research got DNA fragments of ferritin2 gene sizing of about 850 bp, 1200 bp, and 1500 bp. Sequence analysis of the 1200 bp DNA fragment showed the presence of 49 SNPs scattered portion of first intron to fourth exon. Bakung rice genotype had very close proximity to Siam Sintanur and Nipponbare compared with IR64, while Serei and Siputih rice genotypes had a great distance to Siam Sintanur and Nipponbare. The result concluded that Bakung rice genotype may be more tolerant to Fe-toxicity than Siputih and Serei.
PENAMPILAN 54 FENOTIPE GALUR MUTAN KEEMPAT KACANG HIJAU (Vigna radiata L.) LOKAL KAMPAR HASIL MUTASI KOLKISIN Elfrida Oktavia; Herman Herman; Dewi Indriyani Roslim
DINAMIKA PERTANIAN Vol. 28 No. 3 (2013): Jurnal Dinamika Pertanian Volume XXVIII, Nomor 3 2013
Publisher : UIR Press

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This research aimed to evaluate and select the 54 phenotype for fourth mutant galur of Kampar local mung bean resulting kolkisin mutation based on appearance of phenotype and agronomy plant. The research was carried out at Biological farm of FMIPA Riau University from April to September 2013. The randomized Block Design was used with one factor consisting of 54 treatments and 5 repetitions. Of the 54 galurs tested, it was found that 5 galur had the characteristic based on the observed parameter, namely PRCM_2153 galur, PRCM_2353 galur, PRCM_3314 galur, PRCM_3514 galur, and PRCM_3934 galur.
PENILAIAN DAN SELEKSI GALUR KACANG HIJAU (Phaseolusradiata) HASIL KOLKISIN M1 DAN M2 Herman Herman; Fitmawati Fitmawati; Dewi Indriyani Roslim; Fathurrahman Fathurrahman; Okyarni Nuzila
DINAMIKA PERTANIAN Vol. 27 No. 3 (2012): Jurnal Dinamika Pertanian Volume XXVII, Nomor 3 2012
Publisher : UIR Press

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Plant selection of mutant with cholcisin of 507 accessions planted at the experimental farm of FMIPA Biology Riau University. A Completely Randomized Design was used in this research. Observation result for 15 accessions M1 and M2 of mutant mung bean pea had a potential to be developed as a high yielding plant. Based on observation of plant height characteristics for mutant mung bean was obtained the plant height ranging from 89 to 100.5 cm, flowering age ranging from 32 to 34 days (50%), pod age ranging from 37 to 53 days, harvested pod number ranging from 68 to 70 (50% mature), and pod weight ranging from 39-53 g/heaviest plant. For mung bean, cholcisin mutation induction could induct to emerge mutant generation. This mutant had better productivity comparing to local green pea found in Sendayan Kampar Riau before being inducted. Mutant which showed the best result was M1 and M4 colleting big fruit, 80% of mature and little trikom generation.
Co-Authors Adiwirman Dede Aryani Novitasari Della Rinarta Desnilia ' Dita Deanesia Dwi Wulan Elfrida Oktavia Endah Budi Lestari Ermi Ningsih Fadel Nugraha Fathurrahman Fathurrahman Fitmawati ' Fitmawati Fitmawati Herman ' Herman Herman Herman Herman Indah Mulyasari M. Adi Zulkifli Misriyani Misriyani Murtiana Caniago Nazaruddin Nazaruddin Nery Sofiyanti Okyarni Nuzila Rahmi Anandia Rinda Anggini Rini Restiani Rizka Suci Akmando Rotupa Juliana Manurung Roza Elvyra Shinta Oktavia Yolla Putri Ardila Yusfiati Yusfiati