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All Journal Jurnal Sain Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
NLP Indi Dharmayanti
Indonesia Research Center for Veterinary Science Indonesian Agency for Agricultural Research and Development Jalan R.E. Martadinata No. 30, PO Bx 52 Bogor 16114, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : JURNAL BIOLOGI INDONESIA

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EFIKASI VAKSIN INAKTIF BIVALEN AVIAN INFLUENZA VIRUS SUBTIPE H5N1 (CLADE 2.1.3. DAN CLADE 2.3.2) DI INDONESIA Dharmayanti, NLP Indi; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2190

Abstract

ABSTRACTStatus of avian influenza virus subtype H5N1 in Indonesia until 2014 is still endemic in poultry and recorded, there were two types clade of circulating H5N1 namely clade 2.1.3 and the new introduction of lade 2.3.2 since the end of 2012. Both of the clade of avian influenza viruses subtype H5N1 (clade 2.1.3 and 2.3.2) caused the the  AI vaccination program to control of AI in poultry needs to be evaluated.  In this study, we developed a bivalent AI vaccine (which contains clade 2.1.3 and 2.3.2 viruses as a seed vaccine) that   adapted with  the circulation of AI viruses in the field. Result of the study showed that the bivalent vaccine which developed in this study has good efficacy that was challanged with both of AI clade AI and proven to reduce shedding / viral contamination to the environment. It is expected that  the development of bivalent H5N1 vaccine will increase the effectiveness and efficacy of vaccination programs to control highly pathogenic avian influenza disease in Indonesia. Keywords : avian influenza virus, clade, vaccine, bivalent 
VIRUS INFLUENZA NOVEL H1N1 BABI DI INDONESIA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3115

Abstract

ABSTRACTNovel H1N1 influenza virus in Swine in Indonesia. Novel H1N1 influenza virus occurred sinceApril 2009 has caused mortality in human population. In Indonesia, this situation requireintensive surveillance to prevent reassortant probability between the H5N1 virus and novelH1N1 virus. This study conduct preliminary surveillance of novel H1N1 virus circulation byusing Real Time-Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR), that validatedby CDC to detect novel H1N1 virus. Result of this study revealed that the influenza novelH1N1 virus was detected in swine/pigs in Indonesia especially in Bulan island and two individualsample from Kapok slaughter house in Jakarta. These findings showed that in Indonesia thenovel H1N1 virus is not only found in human but also has circulated in swine in Indonesia.Key words: pig/swine, influenza novel H1N1 virus
RESPON TITER ANTIBODI DAN PROTEKSI VIRUS NEWCASTLE DISEASE GENOTYPE I, II, VI DAN VII SEBAGAI VAKSIN TERHADAP INFEKSI ISOLAT VIRUS NEWCASTLE DISEASE CHICKEN/INDONESIA/GTT/11. Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2887

Abstract

ABSTRACTThe Newcastle Disease (ND) virus causes the most important disease in chicken, and very contagiuos in poultry in many countries. In Indonesia the Newcastle Disease or is called tetelo became endemic in chicken currently, the ND virus of genotype VII caused morbidity 100% and mortality 80% in chicken. The fusion and haemaglutinin-neuraminidase protein are involved in adhesion to the surface of hert cells to antibodies. HN and F protein induced by the ND vaccines have neutralizing effects. The aim of this study was to compare the antibody level and protection of several ND vaccine genotypes (genotype I, II, VI and VII) in chicken against ND virus chicken / Indonesia/GTT/11 genotype VII. Our result showed that all genotype ND vaccines (I, II, VI dan VII) produce 100% protection in chicken from clinical and mortality, but the challenge virus excretion in chickens vaccinated with ND vaccine VII genotypes were significantly different (p <0.05) to other groups. Response of antibody in chicken vaccinated ND genotype VII show highest mean titer (GMT 122) compered other ND vaccine. In onclusion, the ND virus chicken/Indonesia/ GTT/11 genotype VII is good to be used as inactive vaccine seed, which appropriate to the field virus circulating at this time.Keywords: virus, Newcastle Disease, genotype, vaccine
SIRKULASI VIRUS AVIAN INFLUENZA H5N1 TAHUN 2010 : VIRUS GENETIC DRIFT MIRIP A/CK/WEST JAVA/PWT-WIJ/2006 DITEMUKAN DI BEBERAPA KABUPATEN DI SUMATRA DAN JAWA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
Co-Authors Ahpas, Ahpas Assadah, Nur Sabiq Atik Ratnawati Bahri S Bambang Ngaji Utomo Darminto . Dewiyanti, Rina Didik Tulus Subekti Dwi Endrawati Dyah Ayu Hewajuli Ermayati, Ermayati Fabeane, Ajeng Fairusya, Nuha Farida Syamsiah, Farida Fitrine Ekawasti, Fitrine Hartawan, R. Hartawan, R. Hewajuli, D.A. Hewajuli, D.A. Hoerudin, Heri Indrawati Sendow Jejen Jaelani Muharam Saepulloh Nepho, Farlin Nuradji, Harimurti Nurcahyo, Raden Wisnu Purwani, Ani Purwanto, Eko Setyo Putri, Ajeng Fabeana R. Indriani Rahmat Setya Adji Ratnawati, A. Ratnawati, A. RISA INDRIANI RISZA HARTAWAN Riza Zainuddin Ahmad Saepullah, Muharam Saepulloh, Muharram Sa’diah, Siti sekarmila, Gita Suryatmiati, Sri Suyatno, Teguh Ukhti, Dwi Rillah Umi Cahyaningsih Wawan Gunawan Winarsongko, Agus yuda pratama