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Uji Efektifitas Ekstrak Etanol Daun Jarak Pagar (Jatropha curcas L.) Terhadap Bakteri Staphylococcus epidermidis Dan Propionibacterium acnes Secara In Vitro Alfi Sapitri; Prima Jaya Nazara; Vivi Asfianti
Herbal Medicine Journal Vol 3 No 2 (2020): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (552.467 KB)

Abstract

Jatropha curcas L. leaves are known as one of the traditional medicinal ingredients. Jatropha leaves contain nutritious substances such as Alkaloids, Flavonoids, Tannins, and Saponins which have the ability as an antibacterial. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak etanol daun jarak pagar terhadap bakteri Stapphylococcus epidermidis dan Propionibacterium acnes secara in vitro. The method used in this study is experimental, including the collection and processing of Jatropha curcas (Jatropha curcas L.) samples, macroscopic and microcospic examinations, phytochemical screening, antibacterial effectiveness testing using diffusion method using disc paper, and using one-way analysis of data analysis methods. (One Way Anova). The results of ethanol extract of Jatropha leaves were able to inhibit bacterial growth ranging from concentrations of 110%, 125%, 250%, 300%, 350% and as a positive control using a tetracycline comparison. This research shows that the higher the concentration, the wider the inhibition zone formed.
Edukasi Masyarakat Mandala dalam Pembuatan Minuman Fermentasi Kombucha Teh Hitam, Teh Hijau, dan Bunga Telang, serta Uji Organoleptik Musa Al Quddusi; Retnita Ernayani Lubis; Alfi Sapitri; Dian Arisetya
Jurnal Pengabdian Mitra Masyarakat Vol 6, No 2 (2026): Edisi Maret
Publisher : Universitas Islam Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/jurpammas.v6i2.14048

Abstract

Penelitian ini bertujuan untuk mengevaluasi dan membandingkan karakteristik organoleptik kombucha yang difermentasi menggunakan tiga jenis bahan dasar: teh hitam, teh hijau, dan bunga telang (Clitoria ternatea). Metode yang digunakan adalah eksperimental laboratorium dengan Rancangan Acak Lengkap (RAL). Proses fermentasi dilakukan selama 8 hari pada suhu ruang dengan penambahan gula 10% sebagai sumber karbon bagi kultur pemula SCOBY (Symbiotic Culture of Bacteria and Yeast). Pengujian organoleptik (uji hedonik) melibatkan 15 panelis tidak terlatih yang menilai parameter warna, aroma, rasa, sensasi karbonasi, dan penerimaan keseluruhan menggunakan skala 1 hingga 5. Data hasil kuesioner dianalisis secara deskriptif berdasarkan perhitungan nilai rata-rata (mean). Hasil pengujian menunjukkan bahwa ketiga sampel dapat diterima dengan baik oleh panelis tanpa adanya penolakan. Kombucha teh hitam meraih tingkat penerimaan keseluruhan yang paling konsisten (rata-rata ~4,1), disusul oleh kombucha bunga telang (~4,1) yang unggul secara signifikan pada parameter visual (warna ungu dari pigmen antosianin) dan mendapatkan penilaian "sangat suka" terbanyak secara individu. Kombucha teh hijau menyusul dengan nilai rata-rata 3,8 yang dipengaruhi oleh profil keasaman yang lebih dominan. Kesimpulannya, inovasi pemanfaatan bunga telang sangat berpotensi untuk dikembangkan lebih lanjut sebagai minuman fungsional komersial karena memiliki daya tarik estetika visual dan keseimbangan profil rasa yang sangat diminati
Project-based biotechnology learning in teacher education to improve pre-service biology teacher higher order thinking skills Retnita Ernayani Lubis; Alfi Sapitri
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.5330

Abstract

Biotechnology learning in higher education is often theoretical and lacks challenges for students to develop higher-order thinking skills (HOTS). In fact, the ability to analyze, evaluate, and create is crucial for prospective biology educators in the 21st century. This study aimed to enhance students' HOTS through the implementation of project-based learning (PjBL) in biotechnology instruction. The subjects were eight Biology Education students at the Islamic University of North Sumatra (UISU) enrolled in a Biotechnology course. A mixed-methods sequential explanatory design with one-group pretest-posttest was employed. Instruments included HOTS tests in the form of essay questions developed based on the revised Bloom's taxonomy in the cognitive domains C4 (analyze), C5 (evaluate), and C6 (create), as well as observation sheets, logbooks, interviews, and Focus Group Discussion. The results showed a significant increase in HOTS scores (pretest=48.75; posttest=82.50; N-gain=0.66, medium category; Cohen's d effect size=2.86). The highest improvement was observed in the creating indicator (C6, N-gain=0.71). Qualitative analysis revealed five themes of HOTS development processes: facing ambiguity as a trigger for analysis, experimental failure as a means of evaluation, collaboration fostering critical argumentation, project freedom nurturing creativity, and metacognitive reflection. The quality of project products (biofertilizers) was positively correlated with HOTS improvement (r=0.82). It is concluded that project-based biotechnology learning effectively enhances students' HOTS. This model is recommended for implementation in Biotechnology courses within Biology Education study programs.