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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Agrikultura Jurnal Natur Indonesia Cropsaver : Journal of Plant Protection Agrovigor: Jurnal Agroekoteknologi Jurnal Penelitian Tanaman Industri Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroteknos Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Penelitian Tanaman Industri (Littri)

DJOKO PRIJONO

Departemen Proteksi Tanaman, Fakultas Pertanian Institut Pertanian Bogor, Kampus IPB Dramaga, Bogor 16680

Author-ID : 255063

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Industrial & Manufacturing Engineering Medicine & Pharmacology

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PERKEMBANGAN DAN REPRODUKSI CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) PADA PAKAN ALAMI DAN SEMIBUATAN Novalia Jelita Sari dan Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 4 No. 2 (2004): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Development and reproduction of Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) on natural and artificial diets. Development and reproduction of Crocidolomia pavonana on natural and artificial diets were studied in the laboratory (25.2 ± 0.9 ï‚°C; 84.6% ± 6.7% RH; ca. 12 h photophase). The natural diets tested were broccoli, cabbage, chinese cabbage, and cauliflower leaves. The artificial diets used were agar–based general lepidopteran diets mixed with red bean or broccoli leaves, and supplemented with vitamins and microbial inhibitors. Observation of insect development was done since the egg stage. After the eggs hatched, larvae were kept singly in plastic cups and fed with appropriate diets. Fifty larvae were used for each diet. Records were kept with regard to the duration of each larval instar, pupal period, and the pupal weight. The emerging adults were paired, and then the number of dead adults and that of eggs laid were recorded daily. On all the natural diets tested, the larval stage of C. pavonana passed through four instars. The egg incubation, total larval developmental, and pupal period of C. pavonana on chinese cabbage were the shortest compared to those on the other natural diets. The pupal weight, however, was the highest on broccoli, followed by that on chinese cabbage, cabbage, and cauliflower. The female fecundity was also the highest on broccoli (average 258 eggs/female) followed by that on chinese cabbage (212), cauliflower (162), and cabbage (102). Broccoli diet also yielded adults with the longest lifespan although the adult lifespan on broccoli was not significantly different from that on the other natural diets, except that of males on cabbage. C. pavonana failed to develop successfully on six kinds of artificial diets tested. The best artificial diet (broccoli–based diet with microbial inhibitors 20% of the normal amount) only yielded two males and five females with deformed wings, but none of the emerging females produced eggs.

AKTIVITAS INSEKTISIDA EKSTRAK DAUN DAN BIJI TEPHROSIA VOGELII J. D. HOOKER (LEGUMINOSAE) DAN EKSTRAK BUAH PIPER CUBEBA L. (PIPERACEAE) TERHADAP LARVA CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: CRAMBIDAE) Muhamad Abizar & Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Insecticidal activity of leaf and seed extracts of Tephrosia vogelii J. D. Hooker (Leguminosae) and fruit extract of Piper cubeba L. (Piperaceae) on the cabbage head caterpillar, Crocidolomia pavonana (L.) (Lepidoptera: Crambidae). Ethyl acetate leaf and seed extracts of Tephrosia vogelii and a solid fraction of ethyl acetate fruit extract of Piper cubeba were evaluated for their insecticidal activity on second-instar larvae Crocidolomia pavonana by a leaf-residue feeding method in the laboratory. Leaf extracts of purple and white-flowered T. vogelii showed the same pattern of component separation on silica gel TLC plate (Rf between 0.21 and 0.94), and likewise the separation of components of seed extracts of purple and white-flowered T. vogelii (Rf between 0.31 and 0.96). All four kinds of T. vogelii extracts showed intense UV-absorbing nonpolar spots (Rf > 0.8). Based on LC50 ratio at day 4, leaf extract of purple-flowered T. vogelii (LC50 0.075%) was 4.30, 2.70, 2.21, and 1.64 times more toxic than fruit extract of P. cubeba, seed extract of white-flowered T. vogelii, seed extract of purple-flowered T. vogelii, and leaf extract of white-flowered T. vogelii, respectively. All T. vogelii extracts were more toxic to C. pavonana larvae than P. cubeba fruit extract. At LC95 level, a mixture of leaf extract of purple-flowered T. vogelii and fruit extract of P. cubeba (5:9, w/w) was more toxic to C. pavonana larvae than each extract tested separately. This extract mixture had synergistic joint action against C. pavonana larvae both at LC50 and LC95 level. Thus, leaf extract of purple-flowered T. vogelii and its mixture with P. cubeba fruit extract are promising to be used for controlling C. pavonana.

GANGGUAN BIOLOGI PADA CROCIDOLOMIA PAVONANA (F.) (LEPIDOPTERA: PYRALIDAE) AKIBAT PERLAKUAN DENGAN EKSTRAK BIJI AGLAIA ODORATISSIMA BLUME (MELIACEAE) Erwin Cuk Surahmat dan Djoko Prijono .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 2 No. 2 (2002): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Biological interferences in Crocidolomia pavonana (F.) (Lepidoptera: Pyralidae) as affected by the treatment with seed extract of Aglaia odoratissima Blume (Meliaceae). The effects of Aglaia odoratissima seed extract on mortality, feeding, development, and reproduction of Crocidolomia pavonana were studied in laboratory. Ground seeds of A. odoratissima were extracted with methanol, the extract obtained was partitioned in n-hexane and 95% aqueous methanol, and the methanol fraction was partitioned further in chloroform and water. The chloroform fraction obtained was used in all tests. In mortality tests, first- and second-instar larvae C. pavonana were fed with extract-treated broccoli leaf discs. The first instars were also used in the test to determine the effect of the test extract on development and fecundity of C. pavonana, while the second instars were also used in antifeedant test. A. odoratissima extract acted relatively slowly and larval mortality mostly occurred in the instar that was given the feeding treatment. This is also reflected by the relatively small difference between LC50 against second+third instar (0.166%) and that against the second instar only (0.175%). The test extract at all concentrations tested also inhibited feeding by C. pavonana larvae. The test extract at 0.471% delayed the development from the first instar to the pupal stage by 2.57 days compared with control. The treatment with the test extract at 0.095-0.471% decreased the fecundity of the females C. pavonana by 8.6-85.6%. Those various biological interferences in C. pavonana caused by the treatment with A. odoratissima extract can result in substantial suppression of the target pest population in the field.

EFIKASI INSEKTISIDA NABATI EKSTRAK DAUN Tephrosia vogelii Hook. TERHADAP Crocidolomia pavonana (F.) dan Plutella xylostella (L.) SERTA PENGARUHNYA PADA Diadegma semiclausum (Hellen) Agustin Zarkani; Djoko Prijono
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 12, No 1 (2010)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.12.1.68-75

[EFICATION OF BOTANICAL INSECTICIDE OF Tephrosia vogelii Hook. f. LEAF EXTRACT AGAINTS Crocidolomia pavonana (F.) AND Plutella xylostella (L.) AND ITS EFFECT TO Diadegma semiclausum (Hellen)]. The active fraction of hexane extract of Tephrosia vogelii (Tv) leaves was evaluated for this insecticidal activity on second-instar larvae of Crocidolomia pavonana and Plutella xylostella as well as for the safety to the adults of Diadegma semiclausum parasitoid. Fraction (fr) 2-4 of Tv from column chromatography (CC) had strong insecticidal activity on C. pavonana and P. xylostella. In the test with C. pavonana, the fraction was more active by feeding than by contact. Based on LC50 at 72 hours since treatment (HST), fr 2-4 CC Tv was 1.8 times more toxic to P. xylostella than to C. pavonana. The fr 2-4 CC Tv showed strong antifeedant effect against C. pavonana larvae. At equal test concentrations, the treatment with fr 2-4 CC Tv caused much lower mortality in D. semiclausum parasitoid adults than in its host larvae, P. xylostella. In contrast, an organophosphate profenofos, included in this study as a positive control, was much more detrimental to D. semiclausum than to P. xylostella. In the semifield experiment, fr 2-4 CC Tv had comparable effect with profenofos and bioinsecticide Bacillus thuringiensis in reducing the population of C. pavonana larvae on broccoli plants.

AKTIVITAS INSEKTISIDA EKSTRAK BUAH CABAI JAWA (Piper retrofractum) TERHADAP Helopeltis antonii (HEMIPTERA: MIRIDAE) GUSTI INDRIATI; DADANG DADANG; DJOKO PRIJONO
Jurnal Penelitian Tanaman Industri Vol 21, No 1 (2015): Maret 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n1.2015.33-40

ABSTRAKHelopeltis antonii merupakan salah satu hama pada tanaman kakao,teh, dan jambu mete yang menyerang pucuk dan buah dengan menusukkanstiletnya untuk mengisap cairan sehingga menyebabkan kerusakan.Penelitian bertujuan menguji toksisitas ekstrak buah Piper retrofractum(cabai jawa) terhadap imago, pengaruh konsentrasi subletal terhadap nimfaketurunan, persistensi terhadap mortalitas dan oviposisi H. antonii.Penelitian dilaksanakan di Laboratorium Fisiologi dan ToksikologiSerangga, Institut Pertanian Bogor dan Laboratorium Proteksi Tanaman,Balai Penelitian Tanaman Industri dan Penyegar Sukabumi, mulai Mei2013 sampai April 2014. Buah mentimun digunakan sebagai inangpengganti untuk perbanyakan serangga uji H. antonii di laboratorium danmedia pengujian. Hasil penelitian menunjukkan kematian imago H.antonii, akibat perlakuan ekstrak P. retrofractum 0,05-0,3%, sudah terjadipada 24 jam setelah perlakuan (JSP). Sementara itu, pada 24 dan 48 JSPterjadi penurunan tingkat kematian H. antonii. Setelah 48 JSP, hanyaterjadi sedikit peningkatan kematian H. antonii. LC 50 dan LC 95 ekstrak P.retrofractum pada 120 JSP masing-masing 0,20 dan 0,49%. Jumlah nimfaketurunan yang dihasilkan pada perlakuan subletal ekstrak P. retrofractum0,203% (LC 50 ) lebih sedikit dibandingkan dengan konsentrasi 0,141%(LC 25 ). Ekstrak P. retrofractum yang dipajankan di bawah sinar mataharihingga 5 hari masih efektif terhadap imago (mortalitas 80%), tetapi tidakefektif dalam menghambat peletakan telur H. antonii. Penghambatanpeletakan telur terhadap imago H. antonii pada perlakuan ekstrak P.retrofractum 0,98% (2 × LC 95 ) yang dipajankan di bawah sinar matahariselama 0 dan 1 hari, dengan indeks penghambatan oviposisi 22,7 dan23,8%. Keefektifan ekstrak P. retrofractum perlu diuji di lapangan untukmenilai kelayakan dalam pengendalian H. antonii.Kata kunci: insektisida botani, mortalitas, oviposisi, persistensiABSTRACTHelopeltis antonii is cocoa, tea, and cashew nuts important pest thatcauses damage by sucking plant sap from shoots and nuts. This study wasconducted to test toxicity of Piper retrofractum fruit extract on adults,sublethal effect on the production nymphal progeny, and persistenceagainst mortality and oviposition of H. antonii. This study was conductedat The Fisiology and Insect Toxicology Laboratory, Plant ProtectionDepartement, Bogor Agricultural University and The Plant ProtectionLaboratory of Indonesian Industrial and Beverage Crops Research Institutefrom Mey 2013 to April 2014. Cucumber was used as a host substitute forrearing the test insect and as the testing medium. The results show thatadult mortality, due to the P. retrofractum leaf extract treatment 0.05-0.3%, has occured at 24 hours after treatment (HAT). Meanwhile, H.antonii mortality has decreased on 24 and 48 HAT. After 48 HAT, only aslight increased in H. antonii mortality. LC 50 and LC 95 of P. retrofractumextract at 120 HAT were 0.20 and 0.49%, respectively. The treatment atsublethal concentrations (LC 25 and LC 50 ) markedly decreased nymphalprogeny number. P. retrofractum extract suspensions at LC 95 and 2 × LC 95exposed under sunlight for 5 days were still effective against H. antoniiadults (80% mortality), but were not effective in females inhibitingoviposition. The oviposition inhibiting activity was observed only in thetreatment with P. retrofractum extract at 2 × LC 95 exposed under sunlightfor 0 and 1 day in which the oviposition deterrency indices were 22.7 and23.8% respectively. Key words: botanical insecticides, mortality, oviposition, persistence

Aktivitas insektisida ekstrak buah Piper aduncum L. (Piperaceae) dan Sapindus rarak DC. (Sapindaceae) serta campurannya terhadap larva Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Yan Yanuar Syahroni; Djoko Prijono
Jurnal Entomologi Indonesia Vol 10 No 1 (2013): April
Publisher : Perhimpunan Entomologi Indonesia

Crocidolomia pavonana is an important pest of Brassicaceous vegetable crops. Botanical insecticides are potential alternatives for the control of C. pavonana pest. Insecticidal activity of Piper aduncum and Sapindus rarak fruit extracts as well as their mixtures was evaluated against C. pavonana larvae in the laboratory. Ethyl acetate P. aduncum (EtPa) extract as well as methanolic S. rarak (MeSr) and aqueous S. rarak (AqSr) extracts were tested separately and in mixtures against second-instar larvae C. pavonana by a leaf-residue feeding method. At the LC95 level - 72 hours after treatment (HAT), EtPa extract was about 14.5 and 12.8 times, respectively, more toxic to C. pavonana larvae than MeSr and AqSr extracts. At the LC95 level, EtPa + MeSr extract mixture (1 : 10 w/w) was about 1.64 times more toxic to the test larvae than EtPa + AqSr extract mixture (1 : 10 w/w). Based on combination index at 48, 72, and 96 HAT, EtPa + MeSr extract mixture at LC50 level as well as EtPa + AqSr extract mixture at LC50 and LC95 levels had additive joint action, whereas EtPa + MeSr extract mixture at LC95 was weakly synergistic. In addition to lethal effect, the treatment with all test materials delayed the development of C. pavonana larvae from second-instar to fourth instar. Thus, EtPa, MeSr, and AqSr extracts as well as the mixtures between EtPa and S. rarak extracts are potential alternatives for the control of C. pavonana pest.

Status dan Mekanisme Resistensi Biokimia Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) terhadap Insektisida Organofosfat serta Kepekaannya terhadap Insektisida Botani Ekstrak Biji Barringtonia asiatica DANAR DONO; SYAFRI ISMAYANA; IDAR IDAR; DJOKO PRIJONO; IKHA MUSLIKHA
Jurnal Entomologi Indonesia Vol 7 No 1 (2010): April
Publisher : Perhimpunan Entomologi Indonesia

An examination of insect resistance was determined by several steps, i.e. standard sensitivity, resistance diagnosis, and determination of resistance level. Each phase was tested with feeding and residue contact methods at glass tube. Resistance ratio (RR) was determined by comparing LC50 value of field population with standard population. Field population of C. pavonana was classified resistant if it had RR 5 4. Biochemistry analysis of resistance was conducted to population of C. pavonana showing resistance to prophenophos insecticide. The activity analysis of acetylcholine esterase (ACHE), esterase, and Glutation Stransferase was done with spectrophotometer method. Insect which are resistant to prophenophos insecticide was tested for its sensitivity to Barringtonia asiatica seed extract. Result indicated that C. pavonana population from Pengalengan showed resistance to prophenophos synthetic insecticide. Using contact test, the highest resistance ratio value was 4.04, while by feeding assay the RR was 2.78. The study on biochemical resistance mechanisms of each field population of C. pavonana showed various activities of enzymatic detoxification. This could be due to the difference in the kind of insecticides exposed to each field population of C. pavonana. Since RR value from the contact test was higher than that of the feeding test, the resistance development of C. pavonana to synthetic insecticides was probably caused by physiological and biochemical changes in insect cuticle rather than the activity of detoxification enzyme. Methanolic seed extract of B. asiatica can be used as an alternative of resistance management of C. pavonana to prophenophos synthetic insecticide.

Potensi Insektisida Pasak Bumi (Eurycoma longifolia Jack) terhadap Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) Eka Chandra Lina; Arneti Aarneti; Djoko Prijono; Dadang Dadang
Jurnal Entomologi Indonesia Vol 6 No 1 (2009): April
Publisher : Perhimpunan Entomologi Indonesia

The research was conducted at Laboratory of Insect Physiology and Toxicology of Plant Protection Department of Bogor Agricultural University from May to July 2008. Leaves, twigs, barks, and roots of Eurycoma longifolia (Pasak bumi) were extracted with hexane, ethyl acetate, and methanol solvens. The extraction was used single method and level method. Insecticidal activity of each extract has been examined in laboratory to major cabbage pests Crocidolomia pavonana using leaf residual method. Methanol root extract from single method and level method caused 100 and 75.5% mortality of insect test respectively. Hexane and ethyl acetate extracts of leaves, twigs, and barks have low insecticidal activity. The antifeedant effect worked at insect test and varied depends on concentration level. Antifeedant effect also contributed to mortality of larvae along with toxicity of each extract.

Pembuatan formulasi ektrak kulit batang Calophyllum soulattri dan aktivitas residu terhadap larva Crocidolomia pavonana Edy Syahputra; Djoko Prijono
Jurnal Entomologi Indonesia Vol 5 No 2 (2008): September
Publisher : Perhimpunan Entomologi Indonesia

One option to lessen the problems arising from the use of synthetic insecticides is to exploit plants that have insecticidal activities, such as species of Calophyllum(Clusiaceae). The study has been conducted to prepare insecticidal formulation from C. soulattri bark extract and to evaluate their residual activity against C. pavonana. Formulation prepared by using emulsifier, solvent, and sticking agent. Colour, acidity and emulsion stability were recorded as physic-chemical character of formulation. The result showed that the formulation of C. soulattri bark extract was stable, and their pH was normal. Bioassay of residual activity was done using leaf-residual method. Formulation of bark extract of 66 EC sprayed in suspension concentration of 1% on potted broccoli plant had residual activity around 70.7%-72% with range of half-lives of 9.4-9.6 days.

Aktivitas residu protein Cry1Ac Pada lahan yang ditanami kapas transgenik-Bt di Bajeng dan Soppeng, Sulawesi Selatan Purnama Hidayat; Djoko Prijono
Jurnal Entomologi Indonesia Vol 3 No 1 (2006): April
Publisher : Perhimpunan Entomologi Indonesia

Soil samples were collected from each plot at the two field sites at 0, 1, 3, and 5 months after planting, and at one-month intervals after harvest for three months. Soil suspension from each sample was mixed with agar based diet (1 g soil/20 ml soil-diet mixture) then was presented to neonate larvae of H. armigera. Two reference standards were used, i.e. pure Cry1Ac protein (0.165 x 104 to 1.436 x 105 ng per ml of the proteindiet mixture) and non-Bt-soil spiked with Cry1Ac protein (0.11 x 104 to 9.573 x 104 ng per ml of the soil-diet-mixture). Larval mortality was recorded at 7 days of treatment. In both reference standards, mortality of H. armigera larvae occurred in a concentrationdependent fashion, i.e. larval mortality increased with the increase in Cry1Ac protein concentration, with LC50 of 0.95 x 104 and 1.65 x 104 ng/ml, respectively, for the pure protein and spiked non-Bt-soil standards. Result of the bioassay of soil samples from the field sites showed the residues from both transgenic cotton soils did not cause any mortality (0%) in the test insects. This indicates that the concentrations of Cry1Ac residue in soil at the study sites were very low (in the second standard, Cry1Ac at a concentration of 0.33 x 104 ng/ml caused about 10% larval mortality).

Co-Authors . Dadang Abdurakhman Abdurakhman Ade Syahputra Agustin Zarkani Ahmad Sifa Arneti Arneti Bambang W. Nugroho Bonjok Istiaji Dadang . DADANG DADANG DADANG DADANG DADANG DADANG DAMAYANTI BUCHORI DANAR DONO Danar Dono Dedek Kusvianti Edy Syahputra Eka Candra Lina EKA CHANDRA LINA Erwin Cuk Surahmat Ferdi Ferdi Gracia Mediana GUSTI INDRIATI Gusti Indriati IDAR IDAR IKHA MUSLIKHA Indah Arastuti Nasution Joni Hidayat latifah K Darusman Latifah K Darusman Latifah Kosim Darusman Maryani, Sri Riski Maya Anggraini Mubin, Nadzirum Muhammad Sigit Susanto Mustopha Ahad Nur Alfi Saryanah Partomuan Simanjuntak PURNAMA HIDAYAT R. Dewi Ratna Wulan RAUF, AUNU Russianzi, Wanda Safri Ishmayana Shinta Puspitasari Sinyong, Kristoforus Sudarmo Sudarmo Surya, Eliana Diana SYAFRI ISMAYANA Syafrida Manuwoto SYAFRIDA MANUWOTO SYAFRIDA MANUWOTO Widodo Widodo Yan Yanuar Syahroni Yogas Dwi Pratiwi Yuswanti, Lucy

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