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Keberhasilan hidup parasitoid Diadegma semiclausum Hellen dan serangga inangnya Plutella xylostella (L.) terhadap aplikasi ekstrak biji srikaya (Annona squamosa L.) Bonjok Istiaji; Djoko Prijono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 15 No 1 (2018): Maret
Publisher : Perhimpunan Entomologi Indonesia

Control of Plutella xylostella L. can be done both biologically and chemically. Biological control is generally done by utilizing plant extracts to kill insect pests. This study aims to determine the effect of the extract of seeds on the mortality and development of parasitoid Diadegma semiclausum Hellen and its host, P. xylostella. The effect of Srikaya seed extract on P. xylostella larvae in the initial stage was tested by foliar rest method, while the residue method on the glass surface was used to test the effect of the extract of seed extract of Srikaya on the death of imago D. semiclausum. The effect of seed extract on parasitization and development of D. semiclausum was performed by exposing P. xylostella larvae contaminated by the extract of srikaya seeds in sublethal concentration (LC5 and LC10) in imago D. semiclausum. The results showed that extract of srikaya seeds at a concentration of 0.0632–0.1% at 24-hour contamination affected the death of the larva P. xylostella and the imago parasitoids D. semiclausum. Imago parasitoids are more susceptible to increased concentration of extracts than P. xylostella larvae. The developmental resistance of P. xylostella by Srikaya seed extract at concentrations used is generally unreal. Similarly, the barriers to the development of parasitoid D. semiclausum in hosts contaminated with sublethal concentrations (LC5 and LC10) of seed extract are generally not significantly different. The presence of Srikaya seed extract in the host also has no significant effect on the extent of parasitization, cocoon length, cocoon width, and cocoon weight. Therefore, there is a possibility to chemically and biologically integrate P. xylostella control.

Pengaruh iradiasi sinar gamma terhadap fertilitas Sternochetus frigidus (Fabricius) (Coleoptera: Curculionidae) pada mangga kuini Ade Syahputra; Mustopha Ahad; Abdurakhman Abdurakhman; Joni Hidayat; Djoko Prijono; Indah Arastuti Nasution
Jurnal Entomologi Indonesia Vol 17 No 1 (2020): Maret
Publisher : Perhimpunan Entomologi Indonesia

One of the recognized and safe quarantine treatments for consumers is the use of gamma-ray irradiation [60Co] at a recommended dose requested by the destination country or international agency. The purpose of this study was to test the effectiveness of a generic dose of gamma ray irradiation for sterilizing mango pulp weevil Sternochetus frigidus (Fabricius) on mangoes cv kuini (Mangifera odorata). A total of 300 kg pulp-weevil-infested mango fruits was collected from West Sumatra and Lampung. First experiment was conducted on April to irradiate fruits collected from West Sumatera and second experiment was on May to irradiate fruits collected from Lampung. In each treatment, a box containing 6-8 M. odorata fruits was irradiated with gamma-ray at doses of 50, 100, 150, 200, 300, 400 Gy with three replications. The treatment of gamma-ray irradiation could reduce feeding activity, normal life of insect, egg production and increase mortality of adults. The results of the first and second experiments showed that gamma ray irradiation was able to influence the development of pupae and adults. There were differences in the normal development of adults in the two experiments. The number of normal development of adults on first experiment was higher than on the second experiment. The irradiated pupae at dose of 100 Gy successfully develop to be adults but the adults are unable to produce eggs. Adults irradiated at 150 Gy are still able to lay eggs with the average of 6 eggs (first experiment) and 2,3 larvae (experiment II), 150 Gy average 6 eggs and do not hatch. Doses at 200 Gy no eggs were produced.

PERKEMBANGAN DAN HAMBATAN MAKAN LARVA Crocidolomia pavonana YANG DIBERI SEDIAAN FRAKSI DIKLORMETAN KULIT BATANG Calophyllum soulattri Syahputra, Edy; Prijono, Djoko
Jurnal Agroteknos Vol 1, No 3 (2011)
Publisher : Jurnal Agroteknos

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ABSTRACKThe objectives of this study were to evaluate the develovment time, and feeding inhibity of Crocidolomia pavonana larvae fed dichloromethana fraction of Calophyllum soulattri bark preparation. Fractionation of C. soulattri fraction was performed with vaccuum liquid chromatography. Bioassay was conducted against C. pavonana larvae by leaf-feeding method. Second-instar C. pavonana larvae were fed extract-treated broccoli leaves for 48 haurs, then were presented with untreated leaves until the surviving larvae reached the fourth-instar stage. The number of dead larvae was recorded. For feeding inhibity, preparation was assayed using leaf-disc choice and no-choice test towards third- instar C. pavonana larvae. The results showed that dichloromethan fraction of C. soulattri barks possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0,06%. The dichloromethan fraction at 0,06%-0,09% could prolong development time of instar II-III larvae by 1,7-2,7 days. In choice and no-choice leaf disc methods, the dichloromethan fraction at 0,02%-0,075% inhibited feeding of instar III larvae by 54,8%-100%. Concerning with their potentiality, further studies are needed to identify insecticidal compounds in those active extracts.Keywords: Calophyllum soulattri, Crocidolomia pavonana, feeding inhibity, insecticidal activity

Tingkat resistensi insektisida emamektin benzoat terhadap ulat krop Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) di Kabupaten Cianjur, Jawa Barat: Resistance level of emamectin benzoate insecticide to Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) in Cianjur Regency, West Java Sinyong, Kristoforus; Mubin, Nadzirum; Prijono, Djoko
Jurnal Entomologi Indonesia Vol 20 No 3 (2023): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.3.247

The cabbage crop caterpillar, Crocidolomia pavonana, that can be very damaging to Brassicaceae plants in Indonesia, particularly in the Cianjur Regency. Farmers in the area have been using synthetic insecticides like emamectin benzoate to control this pest, but it seems that continuous use of these insecticides can lead to resistance in the pest. This research aimed to determine the level of resistance and distribution of emamectin benzoate in C. pavonana in Cianjur Regency. Field populations of C. pavonana were collected from five villages in three districts in Cianjur Regency. As a comparison, a laboratory population was used to determine the resistance ratio. All populations were tested for toxicity to determine the LC50. The results showed that populations of C. pavonana from Cianjur Regency were still susceptible to emamectin benzoate. Treatment at 22.08 í— 10-4 mg a.i/l concentration in 72 hours after treatment showed that the population's mortality was >90%. The resistance ratios of C. pavonana in Cianjur Regency were quite concerning (resistance indocation), with a range of 1.33–2.01 indicating resistance to emamectin benzoate was moderate. Interestingly, the Sindang Jaya population showed the highest resistance ratio. It's worth noting that over 90% of farmers in the region use this insecticide in the field, which supports the idea of resistance to the chemical. This study provided valuable insight into the distribution of insecticide used and resistance levels of C. pavonana in the area support each other.

Tingkat resistensi insektisida emamektin benzoat terhadap ulat krop Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) di Kabupaten Cianjur, Jawa Barat: Resistance level of emamectin benzoate insecticide to Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) in Cianjur Regency, West Java Sinyong, Kristoforus; Mubin, Nadzirum; Prijono, Djoko
Jurnal Entomologi Indonesia Vol 20 No 3 (2023): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.3.247

The cabbage crop caterpillar, Crocidolomia pavonana, that can be very damaging to Brassicaceae plants in Indonesia, particularly in the Cianjur Regency. Farmers in the area have been using synthetic insecticides like emamectin benzoate to control this pest, but it seems that continuous use of these insecticides can lead to resistance in the pest. This research aimed to determine the level of resistance and distribution of emamectin benzoate in C. pavonana in Cianjur Regency. Field populations of C. pavonana were collected from five villages in three districts in Cianjur Regency. As a comparison, a laboratory population was used to determine the resistance ratio. All populations were tested for toxicity to determine the LC50. The results showed that populations of C. pavonana from Cianjur Regency were still susceptible to emamectin benzoate. Treatment at 22.08 í— 10-4 mg a.i/l concentration in 72 hours after treatment showed that the population's mortality was >90%. The resistance ratios of C. pavonana in Cianjur Regency were quite concerning (resistance indocation), with a range of 1.33–2.01 indicating resistance to emamectin benzoate was moderate. Interestingly, the Sindang Jaya population showed the highest resistance ratio. It's worth noting that over 90% of farmers in the region use this insecticide in the field, which supports the idea of resistance to the chemical. This study provided valuable insight into the distribution of insecticide used and resistance levels of C. pavonana in the area support each other.

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