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All Journal HAYATI Journal of Biosciences Agrikultura Bionatura Jurnal Perikanan Kelautan Zuriat Cropsaver : Journal of Plant Protection Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal Entomologi Indonesia Insyst : Journal of Intelligent System and Computation Media Pertanian Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Danar Dono
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Public Health

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The effectiveness of soursop seed (Annona muricata L.) agains Callosobruchus maculatus F. (Coleoptera: Bruchidae) Gilang, Rama Ginanjar; Susniahty, Nenet; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

Callosobruchus maculatus F. (Coleoptera: Bruchidae) one of the main pests that cause damage to mung beans in storage areas. This research was conducted to determine the effective dose from soursop seed (Annona muricata) powder resulting mortality and emphasizing the amount of imago on the first-generation C. Maculatus. This research was conducted at Plant Pest Laboratory, Department of Plant Pests and diseases, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor using experimental method of Complete Random Design (CRD) with 6 treatments and 5 replications. The treatments were tested using 5 doses of A. muricata seed powder with 1 grams, 3 grams, 5 grams, 7 grams, and 9 grams doses, and added with control or without treatment. The results showed that the dose of 5 grams A. muricata seed powder showed the mortality of C. maculatus  by 94% at 8 days after infestation, emphasizing the amount of eggs which is placed  C. maculatus of mungbean seed 77,64%. Emphasis on the emergence of the first generation of imago in the mung bean seeds is 74.67%. The lowest of loss weight mung beed seed amount 1,33%.Keywords: Annona muricata, Callosobruchus maculatus, Soursop Seed Powder, Mortality
Potential Toxicity Of Some Plant Metanol Extracts Against Aedes aegepti (L). Larvae Nanda, Ernaldi Eka; Santosa, Entun; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

Resistance is a decrease in the level of sensitivity of a pest populations that causing a previously effective pesticides becoming ineffective. This case is caused by inappropriate insecticides application, continuous use, only one type of pesticide and usage for long periods of time. There are a lot alternative source of insecticides which is more effective and environmentally friendly. One alternative source of insecticides that can be used for pest control is plants. This study aimed to determine the potential toxicity of methanol extract of N. tabacum, B. asiatica, V. zizanoides, A. indica, and T. vogelli on the mortality and development time of A. aegypti mosquito larvae. The research was conducted at the Laboratory of Pesticides and Environmental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Padjadjaran University, Jatinangor. The experiment was performed using a Completely Randomized Design (CRD) with 6 treatment and 4 replication. The result showed that methanol extracts of the test material were toxic to the larvae A. aegypti with the highest the lowest toxicity were at the concentration of 0.001% i.e treatment of T. vogelli, N. tabacum, B. asiatica, A. indica, and V. zizanoides with the successive rate of mortality (45%, 65%), (37.5%, 60%), (32.5%, 50%) and (32,5%, 47.5%). Besides being toxic, the methanol extract also influenced development of A. aegypti larvae. The pH levels and the dissolved oxygen content of the tested methanol extract solution at the concentration of 0.005% in each treatment are not much different when compare with controls.
Toxicity of four kind plant extracts (Ageratum conyzoides L., Barringtonia asiatica (L.) Kurz., Melia azedarach L., Tephrosia vogelii Hook F.) against brown planthopper (Nilaparvata lugens STAL.) Febritami, Giannisa; Usyati, N; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

One of the main pests that attack rice plants is the brown planthopper (BPH) or Nilaparvata lugens Stål. The negative impact of synthetic insecticides that have been used to control BPH causing increased desire to return to nature with the use of natural materials for plant  protection. One source of natural ingredients that can be used in plant protection can come from plants as a botanical insecticide. Several plant species are known to have the botanical insecticidal activity such as Ageratum conyzoides (Asteraceae), Barringtonia asiatica (Lechytidaceae), Melia azedarach (Meliaceae) and Tephrosia vogelii (Fabaceae). In this research, toxicity testing was performed on extracs of A. conyzoides, B. asiatica, M. azedarach and T. vogelii to BPH, and its phytotoxicity on rice plants. The research was conducted using Completely Randomized Design (CRD) in experimental toxicity test of plant extract and phytotoxicity test on rice plant was done using 2 concentrations of 0.1% and 0.5% in each plant extract, and used 0.5% concentration on anti-oviposition effect. The results showed that T. vogelii methanol extract with 0,5% concentration caused the highest nymphs brown planthopper mortality which was 42,5% and at concentration 0,5% the four plant extract did not cause inhibition of oviposition activity on the BPH and did not cause phytotoxicity in rice plants.
Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Paramita, Hedi; Puspasari, Lindung Tri; Maharani, Rani; Supratman, Unang; Hidayat, Yusup; Meliansyah, Rika; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Testing The Effect of Temperature, Storage Time, The Residual Test of Neem Oil Formulation (Azadirachta indica A. JUSS) and Bitung Formulation (Baringtonia asiatica) to Its Toxicity Against Large Cabbage Heart Caterpillar (Crocidolomia pavonana F) Widayani, Neneng Sri; Haq, Afifah Nashirotul; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

This study aims to determine the effect of temperature and storage time of neem formulation and residual test of neem formulation and bitung formulation to its toxicity on C. pavonana larvae. The formulations tested on the storability and temperature test using the newly prepared, neem formula has been stored for 19 months at room temperature 24.6-31,7 C, and stored in low temperature (4oC) as well as controls. The toxicity of the neem oil formulation was tested at concentrations of 1%, 2%, and 3%. Residual testing was performed by spraying and the residual test samples were taken on days 0,1,2,3,5,7,9,11, and 13 days after application of insecticide spraying. The results showed that storage at low temperatures (temperature 4oC) for 19 months can maintain the toxicity of the neem formulation to C. pavonana larvae. while storage at room temperature for 19 months resulted in a decreased toxicity. The residual activity of the neem formulations of concentration 0.8% and 2.4% ceased to cause the death C. pavonana larvae at the residual age of 13.34 days and 14.66 days. The activity of Bitung formulation concentration of 1.1% and 2.8% ceased to causing death C. pavonana larvae at the residual age of 11.33 days and 16.26 days.Keyword: Azadirachta indica, Baringtonia asiatica, Crocidolomia pavonana, Residue test, Storability test, Temperatures, Toxicity,
Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae Ramadhan, Raden Arif Malik; Widayani, Neneng Sri; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

This study aims to evaluate the bioactivity of the Neem formulation  against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of control, the neem formulaconcentrations of 0.3%, 0.5%, 0.8%, 1.3%, and 2.3%. The test was done by leaf feeding method and tested on C. pavonana instar II. The results showed that the neem formulation 2.3% could increase mortality of C. pavonana larvae with mortalityrate of 95% population and LC  value aqual to 0.82973. Neem formulation can inhibit the development of C. pavonanalarvae from instar I to instar IV, decrease feed intake, and decrease the dry weight of C. pavonana larvae instar IV. Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation
Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae Ramadhan, Raden Arif Malik; Widayani, Neneng Sri; Dono, Danar
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.078 KB)

Abstract

This study aims to evaluate the bioactivity of the Neem formulation  against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of control, the neem formulaconcentrations of 0.3%, 0.5%, 0.8%, 1.3%, and 2.3%. The test was done by leaf feeding method and tested on C. pavonana instar II. The results showed that the neem formulation 2.3% could increase mortality of C. pavonana larvae with mortalityrate of 95% population and LC  value aqual to 0.82973. Neem formulation can inhibit the development of C. pavonanalarvae from instar I to instar IV, decrease feed intake, and decrease the dry weight of C. pavonana larvae instar IV. Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation
Resistance of Nilaparvata lugens to Fenobucarb and Imidacloprid and Susceptibility to Neem Oil Insecticides Danar Dono; Neneng S Widayani; Safri Ishmayana; Yusup Hidayat; Fitri Widiantini; Ceppy Nasahi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.234-244

Abstract

This study aims to monitor and determine the level of resistance of N. lugens to fenobucarb and imidacloprid, to analyse of the specific activities of asetylcholinesterase, Glutation S-Transferase, esterase, and to determine sensitivity of resistant N. lugens to the neem oil. Resistance tests were conducted on a field population from Cipunagara, Subang, West Java, Indonesia, and a standard population originating from Banyuwangi, East Java, that was obtained from the Indonesian Center for Rice Research. Synthetic and neem Insecticides were applied using the leaf-stem dipping method. The resistance ratio obtained by comparing the LC50 value of the field population with the standard. The test insects were considered to be resistant to synthetic insecticide if they had a resistance ratio (RR) of more than 4, indicated resistance if RR ≥1, and susceptible to neem if has RR <1. The results of the research showed that the N. lugens population of Cipunagara indicated resistance to fenobucarb (RR = 2.43), while it was clearly resistance to imidacloprid (RR = 13.95). Specific activities of the acetylcholinesterase and Glutathione S-Transferase of the N. lugens Cipunagara populations were higher than the standard, while the esterase specific activity was lower. These two enzymes are responsible for the resistance of N. lugens to fenobucarb and imidacloprid. Nevertheless, the N. lugens population of Cipunagara was susceptible to neem (RR <1) that never been reported before. Therefore, it is important to know the change in the sensitivity of the target and the metabolic pathway of the insecticide used to manage insect resistance.
Resistance Level of Crocidolomia pavonana Against Profenofos Synthetic Insecticide and its Susceptibility To Azadirachta Indica Seed Extract Danar Dono; Yogas Dwi Pratiwi; Safri Ishmayana; Djoko Prijono
CROPSAVER Vol 1, No 2 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.436 KB) | DOI: 10.24198/cs.v1i2.19912

Abstract

This research was conducted to know resistance level of Crocidolomia pavonana  to a profenofos synthetic insecticide and to know susceptibility of C. pavonana  to Azadirachta indica seed extract. The experiment using two bioassay methods, dry film method and feeding method.  Field population of C. pavonana  obtained from 3 villages in Lembang district i.e.Cibogo, Cikole and Kayu Ambon village. The standard insect has been maintenance more than 20 generations in the laboratory as a comparison.  The result of the experiment indicated that population of C. pavonana  form Cikole, Cibogo and Kayu Ambon village were resistant to profenofos insecticide with resistance ratio (RR)  was low (1< RR ≤ 5).  Resistance Ratio of C. pavonana  from the third village were 4.69 and 4.83; 1.02 and 1.1 and 1.16 and 1.9 respectively (dry film and feeding method). The value of the resistance ratio was greater through the feeding assay compared to the contact assay. This indicated that the mechanism of C. pavonana resistance was likely to occur mainly in the digestive tract of insects. Enzime activity of the different sample population of C. pavonana indicated variation in their activity. This was indicated that every sample from different field could develop different mechanism of resistance. The toxicity study of A. indica  seed extract to C. pavonana  indicate that the field population of C. pavonana  from the third village was susceptible to seed extract of A.indica  with value RR of less than one point. Therefore seed extract of A. indica  could be used as alternative control against C. pavonana  which resistant to profenofos synthetic insecticide.Keywords: Resistance mechanism, Resistance ratio, Synthetic insecticide, Botanical insecticide
Potential Toxicity Of Some Plant Metanol Extracts Against Aedes aegepti (L). Larvae Ernaldi Eka Nanda; Entun Santosa; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.448 KB) | DOI: 10.24198/cs.v1i1.16993

Abstract

Resistance is a decrease in the level of sensitivity of a pest populations that causing a previously effective pesticides becoming ineffective. This case is caused by inappropriate insecticides application, continuous use, only one type of pesticide and usage for long periods of time. There are a lot alternative source of insecticides which is more effective and environmentally friendly. One alternative source of insecticides that can be used for pest control is plants. This study aimed to determine the potential toxicity of methanol extract of N. tabacum, B. asiatica, V. zizanoides, A. indica, and T. vogelli on the mortality and development time of A. aegypti mosquito larvae. The research was conducted at the Laboratory of Pesticides and Environmental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Padjadjaran University, Jatinangor. The experiment was performed using a Completely Randomized Design (CRD) with 6 treatment and 4 replication. The result showed that methanol extracts of the test material were toxic to the larvae A. aegypti with the highest the lowest toxicity were at the concentration of 0.001% i.e treatment of T. vogelli, N. tabacum, B. asiatica, A. indica, and V. zizanoides with the successive rate of mortality (45%, 65%), (37.5%, 60%), (32.5%, 50%) and (32,5%, 47.5%). Besides being toxic, the methanol extract also influenced development of A. aegypti larvae. The pH levels and the dissolved oxygen content of the tested methanol extract solution at the concentration of 0.005% in each treatment are not much different when compare with controls.
Co-Authors Abraham Suriadikusumah Afifah Nashirotul Haq Ainun, Khairunissa Andang Purnama Andhita Nadhirah Argo Utomo C. Pickerling Ceppy Nasahi DJOKO PRIJONO Eka Rahayu Setyaningsih Emelda Anggraini Endah Yulia Endah Yulia Entun Santosa Entun Santosa Entun Santosa - Erawati, Alfira Dewi Ernaldi Eka Nanda Fadhilah Rahmah Aprianti Fathussalam, Muhammad Fauziaty, Muthia Riefka Febritami, Giannisa Fhera Hardiani Fitri Widiantini Fizrul Indra Lubis Frida P. Inangsih - Giannisa Febritami Giffari, Fahri Rijal Gigih Ibnu Prayoga Gilang, Rama Ginanjar Haq, Afifah Nashirotul Haryadi, Dudi Hedi Paramita IDAR IDAR IKHA MUSLIKHA Indah Meutia Arisanti Irman Eka Septiarusli Irpan Permana Irsyad, Andika Muhammad Ivan Febriana Iwan Setiawan Jabbar, Muhammad Aqshal Azizil Kiki Haetami kinya toriyama Lathifah Azizah Lilian Rizkie Lindung Tri Puspasari Maulana, Raihan Rahmat Meliyansyah, Rika Mia Sukma Dewi Mochamad Arief Soleh Muhammad Maksum Mukhamad Agung Yusuf N Usyati Nanda, Ernaldi Eka Narendra, Nathanael Dimas Nasrudin Nasrudin Nasya Nabila Neneng S Widayani Neneng Sri Widayani Neneng Sri Widayani Neneng Sri Widayani Nenet Susniahti Nenet Susniahti Neni Rostini Neni Rostini Nono Carsono Noor Istifadah Nurfadza, Alysha Rianty Nurul Khumaida Paramita, Hedi Purnawan, Pupung Putri, Ghifa Alghifaira R. Arif Malik Ramadhan Raden Arif Malik Ramadhan Rama Ginanjar Gilang Ramadhan, R. Arif Malik Rani Maharani Rani Maharani Ratmaneli Ratmaneli Retno Wulansari Rika Meliansyah Rismanto Rismanto Rohendi Rohendi Rusydan, Muhammad Ardillah Safri Ishmayana Safri Ishmayana Safri Ishmayana Safri Ishmayana Santika Sari Santika Sari Siska Rasiska SIska Rasiska, SIska Sri Hartati Sudarjat Sudarjat Sudarjat Sudarjat Susanti, Rista Susanto, Ainun Nandini Putri Susniahty, Nenet SYAFRI ISMAYANA Syarif Hidayat Syarif Hidayat Syariful Mubarok Tarkus Suganda TATI NURHAYATI Teddy Budiyansyah Toto Sunarto Toto Sunarto Unang Supratman Usyati, N Utami Dwi Ginasti Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Widayani, Neneng Sri Widayani, Neneng Sri Yani Maharani Yenny Mulyani Yogas Dwi Pratiwi Yusuf Hidayat Yusuf Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yusup Hidayat Yuwono, Indra