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Secretory Structure and Histochemistry Tests of Asteraceae Family Members of Medicinal Plants in Walat Mountain Educational Forest Dorly Dorly; Bimo Adi Wiryo; Ismi Nurfaizah; RR. Syafira Nidyasari
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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The diversity of Walat Mountain Educational Forest is not only fauna but also very rich of various kinds of flora such as several kinds of medicinal plants. This research was aimed study the variety of secretory structure and histochemistry of three kinds of medicinal plants found in of Walat Mountain Educational Forest. Plant materials used were billy-goat weed (Ageratum conyzoides), balakacida (Mikania cordata), and monyenyen (Erigeron linifolius).The medicinal plant leaves were made into paradermal and transversal sections, then secretory structures were observed, and the histochemistry of terpenoid, alkaloid, phenol and oil compounds was tested. The results of this research on adaxial and abaxial billy-goat weed leaves were found multicellular glandular trichome and peltate type glandular trichome. Besides, oil drops were also found on billy-goat weed palisade tissue. On adaxial and abaxial bakalacida leaves were found multicellular glandular trichome type 1 and type 2, andpeltate type glandular trichome. Moreover on adaxial and abaxial monyenyen leaves were found multicellular glandular trichome type 1 and type 2. The results of histochemical test on billy-goat weed were the multicellular glandular trichome did not contain terpenoid, alkaloid, phenol, and oil compounds, while on peltate type glandular trichome was positive to terpenoid. Both types of multicellular glandular trichomes on balalakacida plant were positive to terpenoid, alkaloid, and oil compounds, except phenol. Whereas peltate type glandular trchome was positive to alkaloid, phenol, and oil compounds, except terpenoid. Histochemical test results on both types of multicellular glandular trichomes of monyenyen plant were positive to terpenoid and oil compounds.Keywords: secretory structure, histochemical tests, glandular trichome

Keanekaragaman Serangga pada Bunga Tomat (Lycopersicon esculentum Mill.) di Lahan Pertanian Organik Mosi Retnani Fajarwati; Tri Atmowidi; Dorly Dorly
Jurnal Entomologi Indonesia Vol 6 No 2 (2009): September
Publisher : Perhimpunan Entomologi Indonesia

Study on the diversity of insect visitors on tomato flowers were conducted at Bina Sarana Bakti (BSB) organic farm, Cisarua, West Java. Diversity of insects were observed by observational method, an 10 m2 area of tomato plants in the morning, mid-day, and afternoon. Observations were conducted during 20 days. Diversity of visiting insects were analyzed by Shannon diversity and its evenness. Similarity of visiting insects between time of observations were analyzed by Jaccard similarity index. In organic farm, insect visitors of tomato plants consist of six orders belonging to eight species. Insect visitors of tomato flowers were dominated by Thrips sp. (Thysanoptera) and Hylaeus sp. (Hymenoptera). Hylaeus sp. was a main pollinator of tomato plants. Diversity of insect visitors in the morning and mid-day was higher than in the afternoon. Evenness of insect visitors of tomato flowers between observation times were relatively equal.

DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program. Result showed that most of pulasan located in West Java, especially in the area of Bogor. Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti. Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02. Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae.

KEANEKARAGAMAN GENETIK KAPULASAN [NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.] DI JAWA BERDASARKAN MARKA SSR DAN ISSR Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 4 (2020)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Genetic Diversity of Pulasan [Nephelium ramboutan-ake (Labill.) Leenh.] of Java Based on SSR and ISSR Markers. Floribunda 6(4): 117–126. — Pulasan is one of the potential local fruits to be developed. This study aimed to analyze the genetic diversity of pulasan of Java using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers and to obtain information whether primers of the markers could be used to distinguish male and her-maphrodite plants. The results showed that two primers in the SSR markers and seven primers in the ISSR markers produced polymorphic bands. The genomic DNA of the pulasan amplified with SSR markers produced bands 140–500 bp, while those from the ISSR markers were 150–1500 bp. The population of pulasan in Babakan Madang has the highest genetic diversity, while that of Patean is the lowest. Genetic variation of pulasan based on SSR and ISSR markers in the population and among populations have different compositions. Variation in the population is 72% while among the population is 28%. Primers of LML Y6 and LML Y12 from SSR markers and primers of ISSR 2, 3, 4, 5, 6, 8, 9 cannot be used to distinguish male and hermaphrodite pulasan plants. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Keanekaragaman Genetik Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Jawa Berdasarkan Marka SSR dan ISSR. Floribunda 6(4): 117–126. — Kapulasan merupakan salah satu buah lokal yang potensial untuk dikembangkan. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik kapulasan di Jawa dengan menggunakan marka Simple Sequence Repeat (SSR) dan Inter Simple Sequence Repeat (ISSR) serta untuk mendapatkan informasi apakah primer dari marka tersebut dapat dipakai untuk membedakan tumbuhan jantan dan hermafrodit. Hasil penelitian menunjukkan bahwa dua primer pada marka SSR dan tujuh primer pada marka ISSR menghasilkan pita polimorfik. DNA genom kapulasan yang diamplifikasi dengan marka SSR menghasilkan pita-pita dengan ukuran 110–500 bp, sedangkan dari marka ISSR berukuran 150–1500 bp. Populasi kapulasan di Babakan Madang mempunyai keanekaragaman genetik paling tinggi, sedangkan populasi di Patean paling rendah. Variasi genetik kapulasan berdasarkan marka SSR dan ISSR di dalam populasi dan di antara populasi mempunyai komposisi yang berbeda. Variasi di dalam populasi sebesar 72 % sedangkan di antara populasi sebesar 28%. Primer LML Y6 dan LML Y12 dari marka SSR dan primer ISSR 2, 3, 4, 5, 6, 8, 9 tidak dapat digunakan untuk membedakan tumbuhan kapulasan jantan dan hermafrodit.

IS THE FLOWER OF PULASAN (NEPHELIUM RAMBOUTAN-AKE) A PROTOGYNY OR PROTANDRY? Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 7 (2021): Floribunda
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i7.2021.359

Pulasan is an androdioecious plant that has both male and hermaphrodite flowers on separate plants. The objectives of this study were to obtain data about the anatomical structure of pulasan flower and to investigate whether the pistil and the stamens of pulasan flower reach maturity at different times. The anatomical observation was done on compound flowers taken from three male trees and three hermaphrodite trees. Two compound flowers that still in buds, about to bloom and fully bloom were picked from each tree. Flowers were observed in a cross and longitudinal section. The results showed that the sepals of pulasan flower comprised of the uniseriate epidermis and multilayered polyhedral parenchymal cells. Stamen development started from the anther followed by the formation of the filament. The pollen of hermaphrodite pulasan flowers reached maturation earlier than the pistil. The pistil development started from the expansion of meristem cells in the center of the flower and ends with the warp of the stigma.

Anatomi Daun Beberapa Talas Liar (Colocasia esculenta (L.) Schott dari Kabupaten Bogor Dorly Dorly; Yohana C Sulistyaningsih
Caraka Tani: Journal of Sustainable Agriculture Vol 22, No 1 (2007): March
Publisher : Universitas Sebelas Maret

Leaf anatomy characters are commonly stable, however some of them can change because of environmental influence. Identification of those characters are necessary to be conducted by observing anatomical structure of the plants grown out side of the natural habitat. The objective of this research is to examine the stability of leaf anatomy characters of wild taro on their natural habitat and after planted in the dry field. Leaf anatomical observation was conducted by preparing paradermal and transversal sections. The result indicated that leaf anatomical character was the number of palisade layers of Ciju-2 and Jonggol grown out side their natural habitat was stable. Leaf anatomical characters of Ciju-2 collection were palisade thickness, sponge thickness, leaf thickness, stoma density on abaxial surface changed after planted on dry field. Whereas on Jonggol collection significant difference were found on papil height on abaxial surface, stomatal index on abaxial surface, and palisade thickness on dry field and natural habitat.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

Struktur Sekretori pada Physalis angulata sebagai Tumbuhan Obat Muliyah, Evi; Dorly, Dorly; Djuita, Nina Ratna
BIO-SAINS : Jurnal Ilmiah Biologi Vol 2 No 1 (2022)
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v1i2.1797

Physalis angulata merupakan suku Solanaceae yang dapat digunakan sebagai tumbuhan obat. Penelitian ini bertujuan untuk mengidentifikasi struktur sekretori daun dan batang P. angulata. Studi histologi struktur sekretori daun dan batang P. angulata telah dilakukan. P. angulata memiliki struktur sekretori berupa trikoma. Trikoma tersebut merupakan trikoma kelenjar kapitat multiselular yang terdiri atas sel tangkai dan sel kepala. Trikoma tersebut tersebar pada bagian epidermis daun dan batang. Ukuran sel kepala dan tangkai trikoma bervariasi. Kerapatan trikoma tertinggi ditemukan pada epidermis batang.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI DEVI ALVITASARI; TATIK CHIKMAWATI; DORLY Dorly; RUGAYAH RUGAYAH; WISNU HANDOYO ARDI
Reinwardtia Vol. 23 No. 2 (2024)
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

ARDI, W. H. & THOMAS, D. C. 2022. Synopsis of begonia (begoniaceae) from the northern arm of Sulawesi and Sangihe island, Indonesia, including three new species. Edinb. J. Bot 405: 1–50. DOORENBOS, J., SOSEF, M. S. M. & DE WILDE, J. J. F. E. 1998. The sections of Begonia including descriptions, keys and species lists (Studies in Begoniaceae VI). Agric Univ Wageningen Pap 98: 1–266. DUISTERMAAT, H. 1987. A revision of Oryza (Gramineae) in Malesia and Australia. Blumea 32: 157–193. HARTUTININGSIH, M. S., ARDAKA, I. M. & MUSTAID, S. 2007. Flowering Period of 22 Species of Wild Begonias in Bali Botanic Garden. Biodiversitas 8(3): 192–196. HUGHES, M. 2006. Four new species of Begonia (Begoniaceae) from Sulawesi. Edinb J Bot 63(2&3): 191–199. BRC. 2023. Begonia Resource Centre. Faciliated by the Royal Botanic Gardens, Kew. http://padme.rbge.org.uk/begonia. (Accessed 13 December 2023). LIN C. W., THOMAS, D. C., ARDI W. H. & PENG, C. I. 2017. Begonia ignita (sect. Petermannia, Begoniaceae), a new species with orange flowers from Sulawesi, Indonesia. Gard Bull Singapore 69(1): 89–95. MOONLIGHT, P. W., ARDI, W. H., PADILLA, L. A., CHUNG, K. F., FULLER, D., GIRMANSYAH, D., HOLLANDS, R., MAHARDIKA, A., JARA-MUÑOZ, A., KIEW, R., KUMARAGE, L., LEONG, W. C., LIU, Y., O’CONNOR, M., PENG, C. I., PÉREZ, Á. J., PHUTTHAI, T., PULLAN, M., RAJBHANDARY, S., REYNEL, C., RUBITE, R. R., JULIA, S., SCHERBERICH, D., SHUI, Y. M., TEBBITT, M. C., THOMAS D. C. & HUGHES, M. 2018. Dividing and conquering the fastest growing genus: Towards a natural sectional classification of the mega-diverse genus Begonia (Begoniaceae). Taxon 67(2): 267–323. MUNAWAROH, E. & HARTUTININGSIH, M. S. 2018. Upaya konservasi eks-situ famili Begoniaceae dari taman Nasional Bukit Barisan Selatan di Kebun Raya Liwa, Kabupaten Lampung Barat, Provinsi Lampung. Florea 5(1): 44–52. THOMAS, D. C. & ARDI, W. H. 2020. Synopsis of Begonia (Begoniaceae) of southwest Sulawesi and the Selayar Islands, Indonesia, including one new species. Phytotaxa 437(2): 73–96. SBDP. 2023. Sulawesi Begonia Data Portal. Faciliated by the Flora Malaisiana. https://portal.cybertaxonomy.org/flora-malesiana-prospective/node/1 (Accessed 10 December 2023). WIRIADINATA, H. 2013. A New Species of Begonia (Begoniaceae) From South Sulawesi, Indonesia. Reinwardtia 13(5): 445–448.

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