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Induksi Pembungaan Rambutan dengan Aplikasi Paklobutrazol Theresia Prawitasari; Dorly Dorly; Sri Wahyuni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.2845

Rambutan fruit have a great potential to be developed as an exported commodity. The constraint is the existence of biannual bearing characteristic of the plant, which is an alternation of fruit yield between on year and off year. This condition resulted in a problem in product availability continuity. The aim of this research was to induce rambutan plant flowering in the off year with the application of paclobutrazol. There were three doses of paclobutrazol treatment including 0 g/tree, 1.5 g/tree, and 3 g/tree. Bud dormancy as an implication of paclobutrazol application were broken by the application of KNO3 at 20 g/l concentration. The experiment was arranged in completely randomized factorial design 3X2 in triplicate. The result showed that paclobutrazol treatment was able to induce rambutan flowering in the off year. Paclobutrazol treatment significantly increased the number of generative buds. Based on the generative bud percentage, the higher doses of paclobutrazol application the stronger stimulus of flowering. Paclobutrazol treatment also reduced the inflorescences length and increased the chlorophyll content of the flowering support leaves. It seems that application at dose 3 g/tree caused bud dormancy but dose 1.5 g/tree did not. Bud dormancy as a result of paclobutrazol application could be broken by the KNO3 application. The optimum dose to induced rambutan flowering was 1.5 g/tree because this dose seems did not caused bud dormancy and gave a higher number of generative buds compared to 3 g/tree dose.

IDENTIFICATION OF SECRETORY STRUCTURE, HISTOCHEMISTRY AND PHYTOCHEMICAL COMPOUNDS OF MEDICINAL PLANT Hyptis capitata Jacq. Darius Rupa; Yohana Caecilia Sulistyaningsih; Dorly Dorly; Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves. Â The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing Â alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves Â contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.

Secretory Structure and Histochemistry Tests of Asteraceae Family Members of Medicinal Plants in Walat Mountain Educational Forest Dorly Dorly; Bimo Adi Wiryo; Ismi Nurfaizah; RR. Syafira Nidyasari
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

The diversity of Walat Mountain Educational Forest is not only fauna but also very rich of various kinds of flora such as several kinds of medicinal plants. This research was aimed study the variety of secretory structure and histochemistry of three kinds of medicinal plants found in of Walat Mountain Educational Forest. Plant materials used were billy-goat weed (Ageratum conyzoides), balakacida (Mikania cordata), and monyenyen (Erigeron linifolius).The medicinal plant leaves were made into paradermal and transversal sections, then secretory structures were observed, and the histochemistry of terpenoid, alkaloid, phenol and oil compounds was tested. The results of this research on adaxial and abaxial billy-goat weed leaves were found multicellular glandular trichome and peltate type glandular trichome. Besides, oil drops were also found on billy-goat weed palisade tissue. On adaxial and abaxial bakalacida leaves were found multicellular glandular trichome type 1 and type 2, andpeltate type glandular trichome. Moreover on adaxial and abaxial monyenyen leaves were found multicellular glandular trichome type 1 and type 2. The results of histochemical test on billy-goat weed were the multicellular glandular trichome did not contain terpenoid, alkaloid, phenol, and oil compounds, while on peltate type glandular trichome was positive to terpenoid. Both types of multicellular glandular trichomes on balalakacida plant were positive to terpenoid, alkaloid, and oil compounds, except phenol. Whereas peltate type glandular trchome was positive to alkaloid, phenol, and oil compounds, except terpenoid. Histochemical test results on both types of multicellular glandular trichomes of monyenyen plant were positive to terpenoid and oil compounds.Keywords: secretory structure, histochemical tests, glandular trichome

Keanekaragaman Serangga pada Bunga Tomat (Lycopersicon esculentum Mill.) di Lahan Pertanian Organik Mosi Retnani Fajarwati; Tri Atmowidi; Dorly Dorly
Jurnal Entomologi Indonesia Vol 6 No 2 (2009): September
Publisher : Perhimpunan Entomologi Indonesia

Study on the diversity of insect visitors on tomato flowers were conducted at Bina Sarana Bakti (BSB) organic farm, Cisarua, West Java. Diversity of insects were observed by observational method, an 10 m2 area of tomato plants in the morning, mid-day, and afternoon. Observations were conducted during 20 days. Diversity of visiting insects were analyzed by Shannon diversity and its evenness. Similarity of visiting insects between time of observations were analyzed by Jaccard similarity index. In organic farm, insect visitors of tomato plants consist of six orders belonging to eight species. Insect visitors of tomato flowers were dominated by Thrips sp. (Thysanoptera) and Hylaeus sp. (Hymenoptera). Hylaeus sp. was a main pollinator of tomato plants. Diversity of insect visitors in the morning and mid-day was higher than in the afternoon. Evenness of insect visitors of tomato flowers between observation times were relatively equal.

DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program. Result showed that most of pulasan located in West Java, especially in the area of Bogor. Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti. Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02. Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae.

KEANEKARAGAMAN GENETIK KAPULASAN [NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.] DI JAWA BERDASARKAN MARKA SSR DAN ISSR Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 4 (2020)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Genetic Diversity of Pulasan [Nephelium ramboutan-ake (Labill.) Leenh.] of Java Based on SSR and ISSR Markers. Floribunda 6(4): 117–126. — Pulasan is one of the potential local fruits to be developed. This study aimed to analyze the genetic diversity of pulasan of Java using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers and to obtain information whether primers of the markers could be used to distinguish male and her-maphrodite plants. The results showed that two primers in the SSR markers and seven primers in the ISSR markers produced polymorphic bands. The genomic DNA of the pulasan amplified with SSR markers produced bands 140–500 bp, while those from the ISSR markers were 150–1500 bp. The population of pulasan in Babakan Madang has the highest genetic diversity, while that of Patean is the lowest. Genetic variation of pulasan based on SSR and ISSR markers in the population and among populations have different compositions. Variation in the population is 72% while among the population is 28%. Primers of LML Y6 and LML Y12 from SSR markers and primers of ISSR 2, 3, 4, 5, 6, 8, 9 cannot be used to distinguish male and hermaphrodite pulasan plants. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Keanekaragaman Genetik Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Jawa Berdasarkan Marka SSR dan ISSR. Floribunda 6(4): 117–126. — Kapulasan merupakan salah satu buah lokal yang potensial untuk dikembangkan. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik kapulasan di Jawa dengan menggunakan marka Simple Sequence Repeat (SSR) dan Inter Simple Sequence Repeat (ISSR) serta untuk mendapatkan informasi apakah primer dari marka tersebut dapat dipakai untuk membedakan tumbuhan jantan dan hermafrodit. Hasil penelitian menunjukkan bahwa dua primer pada marka SSR dan tujuh primer pada marka ISSR menghasilkan pita polimorfik. DNA genom kapulasan yang diamplifikasi dengan marka SSR menghasilkan pita-pita dengan ukuran 110–500 bp, sedangkan dari marka ISSR berukuran 150–1500 bp. Populasi kapulasan di Babakan Madang mempunyai keanekaragaman genetik paling tinggi, sedangkan populasi di Patean paling rendah. Variasi genetik kapulasan berdasarkan marka SSR dan ISSR di dalam populasi dan di antara populasi mempunyai komposisi yang berbeda. Variasi di dalam populasi sebesar 72 % sedangkan di antara populasi sebesar 28%. Primer LML Y6 dan LML Y12 dari marka SSR dan primer ISSR 2, 3, 4, 5, 6, 8, 9 tidak dapat digunakan untuk membedakan tumbuhan kapulasan jantan dan hermafrodit.

IS THE FLOWER OF PULASAN (NEPHELIUM RAMBOUTAN-AKE) A PROTOGYNY OR PROTANDRY? Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 7 (2021): Floribunda
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i7.2021.359

Pulasan is an androdioecious plant that has both male and hermaphrodite flowers on separate plants. The objectives of this study were to obtain data about the anatomical structure of pulasan flower and to investigate whether the pistil and the stamens of pulasan flower reach maturity at different times. The anatomical observation was done on compound flowers taken from three male trees and three hermaphrodite trees. Two compound flowers that still in buds, about to bloom and fully bloom were picked from each tree. Flowers were observed in a cross and longitudinal section. The results showed that the sepals of pulasan flower comprised of the uniseriate epidermis and multilayered polyhedral parenchymal cells. Stamen development started from the anther followed by the formation of the filament. The pollen of hermaphrodite pulasan flowers reached maturation earlier than the pistil. The pistil development started from the expansion of meristem cells in the center of the flower and ends with the warp of the stigma.

Anatomi Daun Beberapa Talas Liar (Colocasia esculenta (L.) Schott dari Kabupaten Bogor Dorly Dorly; Yohana C Sulistyaningsih
Caraka Tani: Journal of Sustainable Agriculture Vol 22, No 1 (2007): March
Publisher : Universitas Sebelas Maret

Leaf anatomy characters are commonly stable, however some of them can change because of environmental influence. Identification of those characters are necessary to be conducted by observing anatomical structure of the plants grown out side of the natural habitat. The objective of this research is to examine the stability of leaf anatomy characters of wild taro on their natural habitat and after planted in the dry field. Leaf anatomical observation was conducted by preparing paradermal and transversal sections. The result indicated that leaf anatomical character was the number of palisade layers of Ciju-2 and Jonggol grown out side their natural habitat was stable. Leaf anatomical characters of Ciju-2 collection were palisade thickness, sponge thickness, leaf thickness, stoma density on abaxial surface changed after planted on dry field. Whereas on Jonggol collection significant difference were found on papil height on abaxial surface, stomatal index on abaxial surface, and palisade thickness on dry field and natural habitat.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

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