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DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program. Result showed that most of pulasan located in West Java, especially in the area of Bogor. Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti. Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02. Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae.

KEANEKARAGAMAN GENETIK KAPULASAN [NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.] DI JAWA BERDASARKAN MARKA SSR DAN ISSR Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 4 (2020)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Genetic Diversity of Pulasan [Nephelium ramboutan-ake (Labill.) Leenh.] of Java Based on SSR and ISSR Markers. Floribunda 6(4): 117–126. — Pulasan is one of the potential local fruits to be developed. This study aimed to analyze the genetic diversity of pulasan of Java using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers and to obtain information whether primers of the markers could be used to distinguish male and her-maphrodite plants. The results showed that two primers in the SSR markers and seven primers in the ISSR markers produced polymorphic bands. The genomic DNA of the pulasan amplified with SSR markers produced bands 140–500 bp, while those from the ISSR markers were 150–1500 bp. The population of pulasan in Babakan Madang has the highest genetic diversity, while that of Patean is the lowest. Genetic variation of pulasan based on SSR and ISSR markers in the population and among populations have different compositions. Variation in the population is 72% while among the population is 28%. Primers of LML Y6 and LML Y12 from SSR markers and primers of ISSR 2, 3, 4, 5, 6, 8, 9 cannot be used to distinguish male and hermaphrodite pulasan plants. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Keanekaragaman Genetik Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Jawa Berdasarkan Marka SSR dan ISSR. Floribunda 6(4): 117–126. — Kapulasan merupakan salah satu buah lokal yang potensial untuk dikembangkan. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik kapulasan di Jawa dengan menggunakan marka Simple Sequence Repeat (SSR) dan Inter Simple Sequence Repeat (ISSR) serta untuk mendapatkan informasi apakah primer dari marka tersebut dapat dipakai untuk membedakan tumbuhan jantan dan hermafrodit. Hasil penelitian menunjukkan bahwa dua primer pada marka SSR dan tujuh primer pada marka ISSR menghasilkan pita polimorfik. DNA genom kapulasan yang diamplifikasi dengan marka SSR menghasilkan pita-pita dengan ukuran 110–500 bp, sedangkan dari marka ISSR berukuran 150–1500 bp. Populasi kapulasan di Babakan Madang mempunyai keanekaragaman genetik paling tinggi, sedangkan populasi di Patean paling rendah. Variasi genetik kapulasan berdasarkan marka SSR dan ISSR di dalam populasi dan di antara populasi mempunyai komposisi yang berbeda. Variasi di dalam populasi sebesar 72 % sedangkan di antara populasi sebesar 28%. Primer LML Y6 dan LML Y12 dari marka SSR dan primer ISSR 2, 3, 4, 5, 6, 8, 9 tidak dapat digunakan untuk membedakan tumbuhan kapulasan jantan dan hermafrodit.

IS THE FLOWER OF PULASAN (NEPHELIUM RAMBOUTAN-AKE) A PROTOGYNY OR PROTANDRY? Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 7 (2021): Floribunda
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i7.2021.359

Pulasan is an androdioecious plant that has both male and hermaphrodite flowers on separate plants. The objectives of this study were to obtain data about the anatomical structure of pulasan flower and to investigate whether the pistil and the stamens of pulasan flower reach maturity at different times. The anatomical observation was done on compound flowers taken from three male trees and three hermaphrodite trees. Two compound flowers that still in buds, about to bloom and fully bloom were picked from each tree. Flowers were observed in a cross and longitudinal section. The results showed that the sepals of pulasan flower comprised of the uniseriate epidermis and multilayered polyhedral parenchymal cells. Stamen development started from the anther followed by the formation of the filament. The pollen of hermaphrodite pulasan flowers reached maturation earlier than the pistil. The pistil development started from the expansion of meristem cells in the center of the flower and ends with the warp of the stigma.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI ALVITASARI, DEVI; CHIKMAWATI, TATIK; Dorly, DORLY; RUGAYAH, RUGAYAH; ARDI, WISNU HANDOYO
REINWARDTIA Vol 23, No 2 (2024): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

Two new varieties of Begonia hooveriana Wiriad. from Sulawesi are described here, namely Begonia hooveriana var. rubescens Alvitasari and Begonia hooveriana var. sepangensis Alvitasari. They are distinguished based on several morphological characters: base, shape, and size of male flower tepals; peduncle length of female inflorescence; peduncle length, shape, and apex of fruits. A description of two new varieties, identification keys and its distributions are provided here.

Struktur Sekretori pada Physalis angulata sebagai Tumbuhan Obat Muliyah, Evi; Dorly, Dorly; Djuita, Nina Ratna
BIO-SAINS : Jurnal Ilmiah Biologi Vol 2 No 1 (2022)
Publisher : Fakultas Sains dan Teknologi, Universitas Islam As-Syafi'iyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34005/bio-sains.v1i2.1797

Physalis angulata merupakan suku Solanaceae yang dapat digunakan sebagai tumbuhan obat. Penelitian ini bertujuan untuk mengidentifikasi struktur sekretori daun dan batang P. angulata. Studi histologi struktur sekretori daun dan batang P. angulata telah dilakukan. P. angulata memiliki struktur sekretori berupa trikoma. Trikoma tersebut merupakan trikoma kelenjar kapitat multiselular yang terdiri atas sel tangkai dan sel kepala. Trikoma tersebut tersebar pada bagian epidermis daun dan batang. Ukuran sel kepala dan tangkai trikoma bervariasi. Kerapatan trikoma tertinggi ditemukan pada epidermis batang.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI DEVI ALVITASARI; TATIK CHIKMAWATI; DORLY Dorly; RUGAYAH RUGAYAH; WISNU HANDOYO ARDI
Reinwardtia Vol. 23 No. 2 (2024)
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

ARDI, W. H. & THOMAS, D. C. 2022. Synopsis of begonia (begoniaceae) from the northern arm of Sulawesi and Sangihe island, Indonesia, including three new species. Edinb. J. Bot 405: 1–50. DOORENBOS, J., SOSEF, M. S. M. & DE WILDE, J. J. F. E. 1998. The sections of Begonia including descriptions, keys and species lists (Studies in Begoniaceae VI). Agric Univ Wageningen Pap 98: 1–266. DUISTERMAAT, H. 1987. A revision of Oryza (Gramineae) in Malesia and Australia. Blumea 32: 157–193. HARTUTININGSIH, M. S., ARDAKA, I. M. & MUSTAID, S. 2007. Flowering Period of 22 Species of Wild Begonias in Bali Botanic Garden. Biodiversitas 8(3): 192–196. HUGHES, M. 2006. Four new species of Begonia (Begoniaceae) from Sulawesi. Edinb J Bot 63(2&3): 191–199. BRC. 2023. Begonia Resource Centre. Faciliated by the Royal Botanic Gardens, Kew. http://padme.rbge.org.uk/begonia. (Accessed 13 December 2023). LIN C. W., THOMAS, D. C., ARDI W. H. & PENG, C. I. 2017. Begonia ignita (sect. Petermannia, Begoniaceae), a new species with orange flowers from Sulawesi, Indonesia. Gard Bull Singapore 69(1): 89–95. MOONLIGHT, P. W., ARDI, W. H., PADILLA, L. A., CHUNG, K. F., FULLER, D., GIRMANSYAH, D., HOLLANDS, R., MAHARDIKA, A., JARA-MUÑOZ, A., KIEW, R., KUMARAGE, L., LEONG, W. C., LIU, Y., O’CONNOR, M., PENG, C. I., PÉREZ, Á. J., PHUTTHAI, T., PULLAN, M., RAJBHANDARY, S., REYNEL, C., RUBITE, R. R., JULIA, S., SCHERBERICH, D., SHUI, Y. M., TEBBITT, M. C., THOMAS D. C. & HUGHES, M. 2018. Dividing and conquering the fastest growing genus: Towards a natural sectional classification of the mega-diverse genus Begonia (Begoniaceae). Taxon 67(2): 267–323. MUNAWAROH, E. & HARTUTININGSIH, M. S. 2018. Upaya konservasi eks-situ famili Begoniaceae dari taman Nasional Bukit Barisan Selatan di Kebun Raya Liwa, Kabupaten Lampung Barat, Provinsi Lampung. Florea 5(1): 44–52. THOMAS, D. C. & ARDI, W. H. 2020. Synopsis of Begonia (Begoniaceae) of southwest Sulawesi and the Selayar Islands, Indonesia, including one new species. Phytotaxa 437(2): 73–96. SBDP. 2023. Sulawesi Begonia Data Portal. Faciliated by the Flora Malaisiana. https://portal.cybertaxonomy.org/flora-malesiana-prospective/node/1 (Accessed 10 December 2023). WIRIADINATA, H. 2013. A New Species of Begonia (Begoniaceae) From South Sulawesi, Indonesia. Reinwardtia 13(5): 445–448.

TWO NEW VARIETIES OF BEGONIA HOOVERIANA FROM SULAWESI DEVI ALVITASARI; TATIK CHIKMAWATI; DORLY Dorly; RUGAYAH RUGAYAH; WISNU HANDOYO ARDI
Reinwardtia Vol. 23 No. 2 (2024)
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2024.4749

ARDI, W. H. & THOMAS, D. C. 2022. Synopsis of begonia (begoniaceae) from the northern arm of Sulawesi and Sangihe island, Indonesia, including three new species. Edinb. J. Bot 405: 1–50. DOORENBOS, J., SOSEF, M. S. M. & DE WILDE, J. J. F. E. 1998. The sections of Begonia including descriptions, keys and species lists (Studies in Begoniaceae VI). Agric Univ Wageningen Pap 98: 1–266. DUISTERMAAT, H. 1987. A revision of Oryza (Gramineae) in Malesia and Australia. Blumea 32: 157–193. HARTUTININGSIH, M. S., ARDAKA, I. M. & MUSTAID, S. 2007. Flowering Period of 22 Species of Wild Begonias in Bali Botanic Garden. Biodiversitas 8(3): 192–196. HUGHES, M. 2006. Four new species of Begonia (Begoniaceae) from Sulawesi. Edinb J Bot 63(2&3): 191–199. BRC. 2023. Begonia Resource Centre. Faciliated by the Royal Botanic Gardens, Kew. http://padme.rbge.org.uk/begonia. (Accessed 13 December 2023). LIN C. W., THOMAS, D. C., ARDI W. H. & PENG, C. I. 2017. Begonia ignita (sect. Petermannia, Begoniaceae), a new species with orange flowers from Sulawesi, Indonesia. Gard Bull Singapore 69(1): 89–95. MOONLIGHT, P. W., ARDI, W. H., PADILLA, L. A., CHUNG, K. F., FULLER, D., GIRMANSYAH, D., HOLLANDS, R., MAHARDIKA, A., JARA-MUÑOZ, A., KIEW, R., KUMARAGE, L., LEONG, W. C., LIU, Y., O’CONNOR, M., PENG, C. I., PÉREZ, Á. J., PHUTTHAI, T., PULLAN, M., RAJBHANDARY, S., REYNEL, C., RUBITE, R. R., JULIA, S., SCHERBERICH, D., SHUI, Y. M., TEBBITT, M. C., THOMAS D. C. & HUGHES, M. 2018. Dividing and conquering the fastest growing genus: Towards a natural sectional classification of the mega-diverse genus Begonia (Begoniaceae). Taxon 67(2): 267–323. MUNAWAROH, E. & HARTUTININGSIH, M. S. 2018. Upaya konservasi eks-situ famili Begoniaceae dari taman Nasional Bukit Barisan Selatan di Kebun Raya Liwa, Kabupaten Lampung Barat, Provinsi Lampung. Florea 5(1): 44–52. THOMAS, D. C. & ARDI, W. H. 2020. Synopsis of Begonia (Begoniaceae) of southwest Sulawesi and the Selayar Islands, Indonesia, including one new species. Phytotaxa 437(2): 73–96. SBDP. 2023. Sulawesi Begonia Data Portal. Faciliated by the Flora Malaisiana. https://portal.cybertaxonomy.org/flora-malesiana-prospective/node/1 (Accessed 10 December 2023). WIRIADINATA, H. 2013. A New Species of Begonia (Begoniaceae) From South Sulawesi, Indonesia. Reinwardtia 13(5): 445–448.

Studi Pemberian Kalsium untuk Mengatasi Getah Kuning pada Buah Manggis (Garcinia mangostana L.) , Dorly; Indah Wulandari; Roedhy Poerwanto; Darda Efendi; Soekisman Tjitrosemito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 1 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Gamboge is the main problem in mangosteen agribusiness because it is one of the major factors lowering fruit quality. Calcium is one of the important elements that strengthening cell wall; it is which was binding with the pectin as a middle lamella component. The objectives of the research were to study the effect of calcium application on the presence of gamboge spots, physical, and chemical properties of mangosteen fruit. Trial was conducted in a randomized complete block design with three replications in two consecutive years. The calcium source was dolomite, applied in four different dosages, i.e. 0, 18, 24, and 34 ton ha-1 for the fi rst year and 0, 12.5, 15, and 17.5 ton ha-1 for the second year. The results showed that calcium application raised soil pH and calcium content of the soil, exocarp and mangosteen leaves. Dolomite applications using 18 and 24 ton ha-1 in the fi rst year and 17.5 ton ha-1 in the second year were effective to reduce gamboge spots on the outer part of fruit, however they were not effective to reduce gamboge in aril. Dolomite applications did not increase transversal diameter, fruit weight, total soluble solids, total titratable acids, and ratio of total soluble solids with total titratable acids of the fruits.

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