Garuda - Garba Rujukan Digital

SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) Januari 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.

SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) January 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.

Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Puhili, Amelia Luisyane; Chikmawati, Tatik; Djuita, Nina Ratna
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.

Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation] Efah FITRAMALA; Eva KHAERUNNISA; Nina Ratna Djuita Ratna DJUITA; Hadi SUNARSO; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L. For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA. NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0,5 mg/L yang dikombinasikan dengan BA 5 mg/L memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet. [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]

Characteristics and Ideotype Formulation of Pulasan (Nephelium ramboutan-ake) Fruit Landrace from West Java, Indonesia Djuita, Nina Ratna; Hartana, Alex; Chikmawati, Tatik; Dorly,
Makara Journal of Science Vol. 21, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pulasan (Nephelium ramboutan-ake) is a unique tropical fruit, but its morphological variations have not been well characterized. This study was conducted to obtain data on the characteristics of pulasan landrace in West Java, Indonesia, and to formulate the ideotype of pulasan with superior characteristics, such as high fruit weight, sweet taste, and thick and easily peelable aril from the seed. Based on the descriptors of rambutan, 61 fruit variants were observed. The results showed that the average fruit weight was 46.0 g, with the highest at 103.5 g and the lowest at 23.1 g. The °Brix level variation of pulasan fruit was 16.8–29.6, and its vitamin C content was 14.0–24.0 mg/100 g. Five variants with superior fruit characters were identified among the sample collections used in the study

Autecology of Castanopsis argentea (Blume) A.DC. in Telaga Warna Nature Reserve Area, Bogor Regency Dianti Mayadika Putri; Sulistijorini Sulistijorini; Nina Ratna Djuita
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 3 (2022): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.69903

The red list of the International Union for the Conservation of Nature and Natural Resources (IUCN) reported Castanopsis argentea as an endangered species. Studies about autecology on its natural habitat become important to perform species conservation. This study aimed to analyze the population structure, distribution pattern, and environmental factors that influence the presence of C. argentea in the Telaga Warna Nature Reserve. Data was collected in September 2020 by making 21 plots with a single plot. The purposive sampling method was used based on C. argentea representatives to determine plot location. Measurement of environmental factors, including soil sampling was carried out on each plot. Population structure was analyzed based on plant density, and Morisita index determined the distribution pattern. Environmental data were analyzed using PCA with Minitab 19 programs. Our field observation showed that C. argentea seedling has the highest density (1071 ind/ha) and decreased in the mature phase. C. argentea was found to have a clumped distribution pattern with an Id value of 1.03. PCA analysis showed differences in environmental factors that were thought to influence the presence of C. argentea individuals in four growth phases. The highest population structure of C. argentea was found in the growth phase of seedlings and saplings at an altitude of 1400 m asl. The spread population distribution of C. argentea was clumped. The influences of environmental variables on the existence of C. argentea were Mg, Ca, CEC, pH, and soil moisture.

Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Amelia Luisyane Puhili; Tatik Chikmawati; Nina Ratna Djuita
Journal of Tropical Life Science Vol. 6 No. 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.

Diversity and Distribution of Ficus (Moraceae) in The Karst Ecosystem of Bantimurung Bulusaraung National Park Yelastri Yelastri; Sulistijorini Sulistijorini; Nina Ratna Djuita
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.78811

Bantimurung Bulusaraung National Park is an area that has the largest karst ecosystem in Indonesia. Karst is prone to damage and difficult to reuse, so it requires conservation efforts. One of the plant species that can maintain this sustainability is Ficus which acts as key species in karst ecosystems. However, at this time the species is experiencing disturbances, one of which is due to the experience of invasive plants that can threaten the existence of Ficus because the weeds can colonize habitats and are dominant which can change species diversity. This study aimed to analyze the diversity and distribution of Ficus, compare species composition, and analyze environmental factors that affect Ficus spp. in Pattunuang Resort and Bantimurung Resort. Vegetation analysis method with nesting plots placed by purposive sampling and supported with environmental and soil data measurements to determine the factors that indicate the habitat preference of Ficus. We found 18 plant species of Ficus spp. in total. At Resort Pattunuang we found 14 Ficus species with the highest abundance being Ficus sundaica (27.55%), while we recorded 15 Ficus species at Resort Bantimurung with the highest abundance being Ficus ampelas (29.23%). Ficus species were uniformly distributed with a relatively high ratio of species composition in both resorts. Principal Component Analysis (PCA) showed that the presence of Ficus in Pattunuang Resort is influenced by wind speed, soil temperature, air temperature, soil moisture, soil pH, and air humidity. In Bantimurung Resort, the existence of Ficus is influenced by wind speed, soil temperature, soil moisture, and air humidity.

Diversity and Epidermal Characteristic of Lauraceae Leaf in Two Forest Locations, Bogor Regency, West Java Nurul amalia fadhila; Sulistijorini Sulistijorini; Nina Ratna Djuita
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.24406

Lauraceae is a group of tropical plants with a wide distribution. The abundance and species richness of the Lauraceae family includes the under to the upper canopy of the forest. The Lauraceae family can be utilized in various sectors, especially economically. Limiting characters as a significance in the Lauraceae classification was needed to provide additional information regarding taxon grouping. This research aimed to study epidermal characteristics, leaf venation variations, and the diversity of Lauraceae species under different forest environmental conditions. The research procedure involved: collecting ecological field data and morphological identification using a purposive sampling method based on the presence of species. Soil sampling and environmental microclimate were measured on each plot. The anatomical process was carried out by recording characters. Correlation between environmental factors and plant targets used canonical correspondence analysis. The kinship between members of Lauraceae was expressed in a dendrogram. The result of this study showed that Lauraceae could live at various elevations. The analysis data indicated differences in environmental factors that affect the existence of Lauraceae species. Leaf venation studies support clustering in the family using characters such as primary, secondary, tertiary veins, and areola. Observation of leaf venation can be used as a taxonomic significance in the family Lauraceae. Epidermal cell walls in leaf samples are straight, curved shallow, deep, and sharp. Other characters that can be found are anomocytic, laterocytic, and brachyparasitic types of stomata, with stellate and glandless trichomes The anatomical dendrogram separated Lauraceae into clusters on a similarity coefficient scale.

Melissopalynology and Vegetation Analysis Surrounding Sunggau of Giant Honey Bee Apis dorsata in Belitung Regency Dwika Bramasta; Ibnul Qayim; Nina Ratna Djuita; Rika Raffiudin; Ramadhani Eka Putra; RC Hidayat Soesilohadi; Hery Purnobasuki
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1167-1174

The forest conversion into oil palm plantations in Belitung impacts the plant source for pollen and nectar to support the honey bee Apis dorsata. This study aimed to identify the plants used by A. dorsata as pollen sources in honey and bee bread in honey bee nests and to analyze the vegetation composition and structure surrounding the sunggau (artificial nesting site) in Belitung Regency. Honey from A. dorsata was collected from bee nests in sunggau on Kampak Island and Tanjung Rusa. The pollen grains from 20 ml honey were acetolysed and counted until 1,200 grains for each honey sample. In Kampak Island, we found eight pollen types in honey dominated by Rhizophora mucronate mangrove pollen and eleven pollen types in bee bread dominated by Melaleuca cajuputi. The pollen type in honey in Tanjung Rusa was similar to those in Kampak Island, and nine pollen types were found in the bee bread dominated by Elaeis guineensis. The vegetation analysis revealed that mangrove and heath forests in Kampak Island were dominated by Lumnitzera littorea and Melaleuca cajuputi, respectively. The results of this study confirm the bees' notable use of the mangrove ecosystem, which adds conservation value, especially in supporting bee management efforts in Belitung.

Title

Found 38 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 38 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 38 Documents
Search