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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Veteriner Jurnal Ilmu Kesehatan JURNAL KEPERAWATAN
Widjiati Widjiati
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Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Veteriner

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Peningkatan Ekspresi Vascular Endothel Growth Factor dan Mitogen Activating Protein Kinase Plasenta Tikus yang Dipapar Carbon Black ((INCREASING EXPRESSION ON VASCULAR ENDOTHELIAL GROWTH FACTOR AND MITOGEN ACTIVATING PROTEIN KINASE IN PLACENTA RATS EXPOSE Viski Fitri Hendrawan; Widjiati Widjiati; Suherni Susilowati; Pudji Srianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.745 KB) | DOI: 10.19087/jveteriner.2017.18.1.59

Abstract

Carbon black is one of the components of particulate matter (PM), known as cytotoxic, causing inflammationin the respiratory and cardiovascular system, and able to pass through the placental barrier, which may affect the fetus. The effects caused by pollutants depend on the intensity and time length of the exposure.The objective of this study is to identify Vascular Endothelial Growth Factor (VEGF) and Mitogen Activating Protein Kinase (MAPK) as an indicator of maternal placental inflammation in pregnant rats (Rattus norvegicus) exposed to carbon black with immunohistochemical technique. This study was a laboratory experiment with three treatment groups and seven replications using pregnant female white rats (Rattus norvegicus). Pregnant female rats were exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 during pregnancy of day 6 up to day 11. The next phase was the observation and examination of the expressions of VEGF and MAPK by immunohistochemical staining toward placenta of the white rats exposed to carbon black. The result of the study showed an increase in the expression of VEGF in the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3 and 1064 mg/m3 per inhale during the mid-period (6-11) of pregnancy. In addition, there was also an increasein the expression of MAPK on the placenta of the rats exposed to carbon black with a dosage of 523 mg/m3and 1064 mg/m3 per inhale during the mid-period (day 6-11) of pregnancy. In conclusion, the expression of VEGF and MAPK are increased along with the increased dosage of carbon black and age of gestation. ABSTRAK Carbon black merupakan salah satu komponen partikulat matter (PM) yang diketahui bersifat sitotoksik serta menyebabkan terjadinya inflamasi pada sistem pernapasan dan kardiovaskular serta dapat melewati barier plasenta fetus sehingga dapat memengaruhi fetus. Efek yang ditimbulkan oleh polutan tergantung dari besarnya paparan dan lama waktu paparan. Penelitian ini bertujuan untuk mengidentifikasi Vascular Endothel Growth Factor (VEGF) dan Mitogen Activating Protein Kinase (MAPK) sebagai indikator imflamasi pada plasenta maternal tikus (Rattus norvegicus) yang dipapar carbon black dengan teknik imunohistokimia. Penelitian ini dilakukan dengan tiga kelompok perlakuan dan tujuh kali ulangan yang menggunakan tikus betina bunting. Tikus betina bunting dipapar dengan carbon black dosis 523 mg/m3 dan 1064 mg/m3 selama kebuntingan hari ke-6 sampai 11. Tahap berikutnya adalah pengamatan dan pemeriksaan ekspresi VEGF dan MAPK dengan pewarnaan imunohistokimia terhadap plasenta tikus putih yang telah dipapar dengan carbon black. Hasil penelitian adalah terjadi peningkatan ekspresi VEGF pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan (6-11) kebuntingan. Selain itu terjadi peningkatan ekspresi MAPK pada plasenta tikus yang dipapar carbon black dosis 523 mg/m3 dan 1064 mg/m3 per inhalasi selama masa pertengahan(6-11) kebuntingan. Simpulan penelitian ini adalah ekspresi VEGF dan MAPK meningkat seiring dengan peningkatan dosis carbon black dan umur kebuntingan.
Pemberian Buah Merah (Pandanus conoideus Lam) sebelum Dipapar Timah Hitam Menekan Ekspresi Caspase-8 dan Jumlah Sel Hofbauer Mencit (Mus musculus) Bunting (THE PROVISION OF RED FRUIT (Pandanus conoideus Lam) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Ika Wahyuni; Widjiati Widjiati; Sri Pantja Madyawati; Fedik Abdul Rantam
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.904 KB) | DOI: 10.19087/jveteriner.2017.18.1.128

Abstract

The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.
Co-Authors Dwi Puji Wijayanti Fedik Abdul Rantam Hermanto Hermanto Hermanto Tri J Hermanto Tri Joewono Ika WAHYUNI Pipit Sri Estuning Rahayu Pudji Srianto Sri Pantja Madyawati Suherni Susilowati Virki Widoyanti Viski Fitri Hendrawan