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All Journal The Journal of Experimental Life Sciences (JELS) AL KAUNIYAH El-Hayah : Jurnal Biologi El-QUDWAH Prosiding Seminar Biologi Jurnal Biodjati Scripta Biologica Berkala Penelitian Hayati
Evika Sandi Savitri
UNIVERSITAS ISLAM NEGERI MAULANA MALIK IBRAHIM MALANG
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Growth and Flavonoid Content of Gynura procumbens (Lour) Merr. in Different Soil Water Content Savitri, Evika Sandi; Khusnia, Farrikhatun
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4446

Abstract

Gynura procumbens contains tannins, saponins, steroids, triterpenoids, essential oils and flavonoids which have bioactivity as a medicine for various diseases. One effort to obtain the quality of medicinal plants uses management of soil water content. The opti-mum of soil water content will support optimal growth of a plant. Whereas in conditions of low soil water content resulted in the induc-tion of the production of secondary metabolites as a self-defense sys-tem. This study aimed to determine the effect of soil moisture content on the growth and total content of plant flavonoids G. procumbens. This study was carried out experimentally using a one-factor com-plete randomized design (CRD) consisting of 4 treatments with five replications. The treatment used is the difference in soil water content of 40%, 60%, 80% and 100% field capacity. The data obtained in the form of quantitative data will be analyzed by One Way ANOVA Test. Measurement of the content of total flavonoid compounds was car-ried out using UV-Vis spectrophotometer analysis. The results of the study indicate the influence of soil moisture level on the growth and total content of G. procumbens. Parameter growth of fresh weight, dry weight, and root G. procumbens showed an increase in 40% soil water content. The flavonoid content showed the highest total flavo-noid content 18.884 mg/g in the 40% field capacity soil water content 
Phytochemistry Screening and Antioxidant Activities of Extract Pomegranate, Grape, Fig, and Olive in the Various Solvent Savitri, Evika Sandi; Holil, Kholifah; Resmisari, Ruri Siti
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.13424

Abstract

The active compounds of grape, pomegranate, olive, and fig have anthocyanins that potential as antioxidant are flavonoids. Flavonoids have potential as antioxidant  to prevent and therapy various oxidative stress and related diseases. This study aimed to examine the antioxidant activity of a combination of pomegranate, grape, fig, and olive extracts using the DPPH (diphenyl-2-picrylhydrazyl) method.  The maceration method used was maceration of dry Simplicia with methanol 95% solvent, fresh maceration with 95% methanol and dry Simplicia with 95% ethanol solvent. The results of the phytochemistry test showed several compounds found in the extract combination pomegranate, grapes, fig, and olives such as polyphenol, flavonoid, tannin, steroid/triterpenoid. The result of the antioxidant test showed the fresh maceration 95% methanol showed higher results with the IC50 of 25.22 with a potent antioxidant activity category.
The Genetic and Morphoagronomy Character Diversity of Black Soybean Plant (Glycine soja (L.)): Responses to Mutation Induction by Gamma Rays Irradiation and Ethyl Methane Sulfonate Fauziah, Shaddiqah Munawaroh; Savitri, Evika Sandi; Arumingtyas, Estri Laras
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.02

Abstract

Indonesia has a high dependence on imports of black soybeans. National black soybean needs continue to increase. Therefore, some efforts are needed to increase soybean production. Genetic resources of Black Soybeans with high diversity is also needed to develop superior varieties. One of the ways to increase soybean production is by mutation induction. Therefore, genetic resources of Black Soybeans with high diversity is needed to develop superior varieties. Mutagenesis using gamma rays and EMS can be used to increase genetic diversity. Doses used in this research were 1000 Gy gamma rays, 1% EMS, and combinations. This research was using a completely randomized design with morphoargonomy and molecular parameters. Morphoagronomy parameters observed were plant height, leaf area, seed weight, number of leaves, number of pods, and number of branches. ISSR markers with four primers (UBC 888, ISSR3, UBC876, and UBC889) were used to determine genetic variation of Black Soybeans. Based on the analysis of variance (ANOVA), it was shown that all parameters were able to give an effect on the morphoagronomy of black soybean plants. Gamma rays 1000 Gy were more effective in improving morphoagronomy of the plant in terms of plant height, seed weight, number of leaves, number of pods, leaf area, and number of branches. UBC 888 was the most effective primer to identify the genetic diversity of black soybeans that have been given mutation treatments.   Keywords: Black soybean, EMS, gamma rays, genetic variation, morphoagronomy.
Co-Authors AINIYATUL FIKRIYAH Eko Budi Minarno Estri Laras Arumingtyas Farrikhatun Khusnia Kholifah Holil Khusnia, Farrikhatun Rahmi Annisa Retno Susilowati Ruri Siti Resmisari Shaddiqah Munawaroh Fauziah Shinta