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All Journal Jurnal Veteriner AGRIEKONOMIKA JURNAL PETERNAKAN Livestock and Animal Research Jurnal Litbang Provinsi Jawa Tengah Jurnal Agripet
Limbang Kustiawan Nuswantara
Faculty Of Animal And Agricultural Sciences, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education Public Health Social Sciences Veterinary

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Journal : Jurnal Agripet

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Kandungan Serat Kasar, Kecernaan Serat Kasar, dan Fermentabilitas Bonggol Singkong yang Difermentasi Menggunakan Aspergillus niger Aris Budi Prasetyo; Baginda Iskandar Moeda Tampoebolon; Limbang Kustiawan Nuswantara
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.24805

Abstract

ABSTRACT. Tujuan penelitian ini adalah untuk mengkaji pengaruh aras konsentrasi Aspergillus niger dan lama waktu pemeraman yang berbeda terhadap kandungan serat kasar, kecernaan serat kasar, dan fermentabilitas bonggol singkong secara in vitro. Percobaan didesain menggunakan rancangan acak lengkap pola faktorial (RALF) 3x3 dengan 3 ulangan. Fermentasi menggunakan kapang Aspergillus niger dengan 3 aras konsentrasi (A0: 0%, A1: 2,5% dan A2: 5%) dan 3 lama pemeraman (T0: 0 hari, T1: 2 hari dan T2:4 hari). Variabel yang dikaji adalah kandungan serat kasar, kecernaan serat kasar, asam lemak terbang/volatile fatty acid (VFA) parsial berupa asam asetat, propionat, butirat serta VFA total. Data dianalisis ragam kemudian dilanjutkan dengan uji wilayah berganda Duncan. Hasil penelitian menunjukkan terdapat pengaruh interaksi (p0,05) antara aras starter dan lama waktu pemeraman yang berbeda terhadap kandungan serat kasar, kecernaan serat kasar dan fermentabilitas secara in vitro bonggol singkong yang difermentasi dengan kapang Aspergillus niger. Pada kombinasi perlakuan A2T2 menghasilkan kadar serat kasar terendah (20,12%), peningkatan kecernaan serat kasar tertinggi (41,00%), peningkatan VFA parsial tertinggi meliputi asam asetat (58,40%), propionat (26,16%), butirat (12,73%) dan VFA total tertinggi (95,33%). Berdasarkan hasil penelitian disimpulkan bahwa kombinasi perlakuan aras starter Aspergillus niger dan lama pemeraman dapat meningkatkan kecernaan serat kasar, produksi VFA parsial dan VFA total, serta menurunkan serat kasar bonggol singkong. Kecernaan serat kasar, produksi VFA parsial dan VFA total terbaik, serta kandungan serat kasar bonggol singkong terendah terjadi pada kombinasi perlakuan A2T2 dengan penggunaan aras Aspergillus niger 5% dan lama pemeraman 4 hari.(Crude fiber content, crude fiber digestibility and fermentability of fermented Cassava cobs using Aspergillus niger)ABSTRAK. The purpose of this study was to determine the effect of Aspergillus niger levels and fermentation duration on crude fiber content, crude fiber digestibility, and fermentability in vitro cassava cobs. This experiment used a completely randomized design with 3x3 factorial pattern and 3 replications. Fermentation used 3 Aspergillus niger levels (A0: 0%, A1: 2,5% dan A2: 5%) and 3 duration (T0: 0 days, T1: 2 days and T2: 4 days). Observed variables were crude fiber content, crude fiber digestibility, partial volatile fatty acid (VFA) involving acetate, propionate, and butyrate, and total VFA. Data were analyzed using the analysis of variance, then followed by Duncans Multiple Region Test. The results showed that there was an interaction (p0.05) between different starter levels and the fermentation duration on crude fiber content, crude fiber digestibility, and fermentability in vitro cassava cobs. The A2T2 treatment combination shows the lowest level of crude fiber content (20.12%), the highest level of crude fiber digestibility (41.00%), the highest level of acetate (58.40%), propionate (26.16%), and butyrate (12.73%), and the highest total VFA (95.33%). It can be concluded that the combination of A. niger levels and fermentation duration can increase crude fiber digestibility, partial VFA, and total VFA, as well as reducing crude fiber content of cassava cobs. The highest level of crude fiber digestibility, partial VFA, and total VFA, and the lowest crude fiber contents occurred in the combination of A2T2 treatment using 5% level of A. niger with 4 days of fermentation duration.
Kecernaan, Fermentabilitas dan Produksi Protein Mikrobia Secara In Vitro pada Complete Feed Berbasis Pelepah Sawit Fermentasi Limbang Kustiawan Nuswantara; Eko Pangestu; Sunarso Sunarso; Marry Christiyanto
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20554

Abstract

ABSTRACT. Penelitian bertujuan mengetahui kualitas complete feed dengan level pelepah sawit fermentasi berdasarkan kecernaan bahan kering, bahan organik, produksi N-NH3, produksi volatile fatty acids (VFA) dan produksi biomassa protein mikrobia serta protein total secara in vitro. Materi yang digunakan adalah complete feed tersusun atas konsentrat dan pelepah sawit fermentasi dengan berbagai level yaitu 0, 10, 20 dan 30%. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan 4 perlakuan complete feed dengan level pelepah sawit fermentasi yang berbeda. Data diolah menggunakan sidik ragam yang dilanjutkan dengan uji beda wilayah berganda Duncan. Hasil penelitian menunjukkan bahwa complete feed dengan level pelepah sawit fermentasi yang berbeda berpengaruh nyata (p0,05) terhadap kecernaan bahan kering dan bahan organik, produksi N-NH3, produksi VFA, dan produksi protein total, sedangkan pada biomassa protein mikrobia tidak terdapat perbedaan yang nyata (p0,05). Rata-rata nilai kecernaan bahan kering pada perlakuan T0, T1, T2 dan T3 adalah 69,59; 71,9; 69,05; dan 62,58%. Rata-rata nilai kecernaan bahan organik pada perlakuan T0, T1, T2 dan T3 adalah 63,59; 63,15; 65,50; 52,66 %. Rata-rata produksi VFA pada perlakuan T0, T1, T2 dan T3 sebesar 105,8; 142,7; 136,4; dan 135,7 mM. Rata-rata produksi NH3, biomassa protein mikrobia dan produksi protein total pada perlakuan T0, T1, T2 dan T3 berturut-turut adalah 6,48mM, 15,04mg/ml;, 34,10mg/g; 7,36mM, 15,75mg/ml, 23,72mg/g; 8,18mM, 12,59mg/ml, 33,72mg/g); dan 6,60mM, 15,31mg/ml, 40,80mg/g. Berdasarkan hasil penelitian disimpulkan bahwa penggunaan pelepah sawit fermentasi dengan level 20% dalam complete feed menghasilkan produksi VFA, kecernaan bahan kering dan bahan organik yang cukup baik sehingga dapat menjadi pakan alternatif sumber serat pengganti rumput.(Digestibility, fermentability and in-vitro production of microbial protein on complete feed based on fermented palm frond) ABSTRAK. This study aimed to determine the quality of a complete feed containing fermented palm fronds based on the digestibility of dry matter, organic matter, N-NH3, VFA, microbial protein biomass, and total protein in vitro. The material used was complete feed composed of concentrates and fermented palm fronds at various levels, i.e., 0, 10, 20, and 30%. The experiment was conducted as a completely randomized design (CRD) with four complete feed treatments containing different levels of fermented palm fronds. The data were processed using analysis of variance, followed by Duncans multiple range test. The results demonstrated that the complete feed with different levels of fermented palm fronds had a significant effect (p0.05) on the digestibility of dry matter and organic matter, N-NH3 production, essential fatty acids production, and total protein production, whereas there was no significant difference (p 0.05) on microbial protein biomass. The average dry matter and organic matter digestibility values of T0, T1, T2, and T3 treatments were 69.59; 63.59, 71.9; 63.15, 69.05; 65.50, and 62.58%; 52.66% respectively. The average production of volatile fatty acids of T0, T1, T2, and T3 treatments were 105.8; 142.7; 136.4; and 135.7 mM. respectively, while the average N-NH3 production, microbial protein biomass, and total protein production of the T0, T1, T2, and T3 treatments were 6.48, 7.36, 8.18, 6.60 mM; 15.04, 75, 12.59, 15.31 mg/ml; and 34.10, 23.72, 33.72, 40.80 mg/g. In conclusion, the use of fermented palm fronds at a 20% level in complete feed gave the best result in the production of volatile fatty acids, improved digestibility of dry matter, and organic matter, so it can be used as an alternative feed to replace grass fiber.
Komponen Serat Sabut Kelapa yang Difermentasi Menggunakan Mikroba Pencerna Serat dari Rumen Kerbau Limbang Kustiawan Nuswantara; Sunarso Sunarso; Mukh Arifin; Agus Setiadi
Jurnal Agripet Vol 20, No 1 (2020): Volume 20, No. 1, April 2020
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v20i1.15545

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mengkaji pengaruh perbedaan lama pemeraman dan aras starter mikroba pencerna serat dari rumen kerbau terhadap komponen serat dan perubahan struktur jaringan sabut kelapa fermentasi. Percobaan menggunakan rancangan acak lengkap pola faktorial 3x3 dan 4 ulangan. Fermentasi sabut kelapa menggunakan mikroba pencerna serat dari rumen kerbau sebagai starter dengan perlakuan aras starter (0, 2,5 dan 5%) dan lama peram (0, 7 dan 14 hari). Parameter yang diamati adalah komponen serat dan perubahan struktur jaringan. Data dianalisis menggunakan analisis ragam, dilanjutkan uji jarak berganda Duncan. Hasil penelitian menunjukkan tidak terdapat pengaruh interaksi (P0,05) antara perbedaan aras starter dan lama peram terhadap kadar neutral detergent fiber (NDF), acid detergent fiber (ADF), hemiselulosa, selulosa dan lignin. Kesimpulan penelitian ini adalah penurunan kadar komponen serat serta peningkatan kerusakan jaringan seiring dengan peningkatan aras starter dan lama pemeraman. Hal ini disebabkan oleh penurunan kadar selulosa dan lignin sedangkan kadar hemiselulosa tidak dipengaruhi. Hal ini didukung oleh pengamatan terhadap perubahan struktur jaringan.(Fiber components of fermented coconut husk using fibre degrading microbes from buffalo rumen)ABSTRACT. This study aimed to examine the effect of different fermentation periods and levels of fiber-degrading microbial starter from buffalo rumen fluid on fiber component and tissue structure alteration of coconut husk. The experimental design used was factorial randomized complete 3x3 and 4 replications. The coconut husk fermentation was using fiber-degrading microbial obtained from buffalo rumen fluid as a starter with different treatments of starter levels (0, 2.5 and 5%) and fermentation periods (0, 7 and 14 days). Parameters observed were fiber component and tissue structure alteration. The data were analyzed by analysis of variance and continued by Duncans Multiple Range Test. The results of this research showed that there was no interaction effect (P0.05) between starter level and fermentation period on NDF, ADF, hemicellulose, cellulose and lignin contents. The conclusion of this research was decreased fiber component content and damage to tissue structure of coconut husk along with increased starter level and fermentation period. This is caused by decreased levels of cellulose and lignin while hemicellulose levels were not affected. This is supported by observations of changes in tissue structure alteration.
Co-Authors Agung Purnomoadi Agus Setiadi Aldonny Nurdiansyah Aris Budi Prasetyo Baginda Iskandar Moeda Tampoebolon DIANA DELVIA NANDA Eko Pangestu Eko Susilo Farah Faradilla Isnaini Nurkhasanah Joko Mariyono Kadhung Prayoga Marry Christiyanto Mukh Arifin Okta Filasari Siswanto Imam Santoso Siti Aminah Siti Athiya Wibowo Siwi Gayatri Sunarso . Suryani Nurfadillah