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Gambaran Cemaran Kapang Kontaminan Pada Buah Pepaya (Carica papaya L) Selama Penyimpanan Yeti Eka sispita Sari; Dita Artanti
PEDAGO BIOLOGI Vol 10 No 2 (2022)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pb:jppb.v10i2.17633

Abstract

ABSTRAK Buah pepaya (Carica papaya L) merupakan jenis buah yang menjadi primadona bagi masyarakat karena rasanya yang manis dan kaya akan vitamin serta gizi. Pada kenyataannya kualitas buah pepaya segar maupun saat proses panen sampai pendistribusian tidak langsung bisa dikonsumsi karena adanya cemaran mikroorganisme penghasil mikotoksin yaitu kapang. Hal ini menyebabkan pepaya menjadi cepat busuk dan berbahaya apabila terus dikonsumsi. Tujuan penelitian ini untuk mengetahui gambaran cemaran kapang kontaminan pada buah pepaya (Carica papaya L).. Jenis penelitian yang digunakan adalah deskriptif. Sampel uji sebanyak 30 sampel pepaya yang sudah disimpan pada suhu ruang selama 5 hari dengan luka fisik di buah. Kapang yang tumbuh akan dilakukan pemeriksaan langsung secara mikroskopis menggunakan pewarna Lactophenol Cutton Blue. Berdasarkan hasil penelitian dari 30 sampel buah pepaya (Carica papaya L) ditemukan tiga jenis kapang dengan persentase masing-masing yaitu Aspergillus flavus (60%), Rhizopus oryzae (23,3%) dan Aspergillus niger (16,7%). Hal ini menunjukkan bahwa buah pepaya yang dibiarkan pada suhu ruang dengan adanya luka fisik dapat memicu pertumbuhan kapang. Kata kunci : Buah Pepaya, Aspergillus flavus, Rhizopus oryzae, Aspergillus niger ABSTRACTPapaya fruit (Carica papaya L) is a type of fruit that is a prima donna for the community because of its sweet taste and rich in vitamins and nutrients. In fact, the quality of fresh papaya fruit and during the harvesting process until distribution can not be consumed directly due to the contamination of mycotoxin-producing microorganisms, namely mold. This causes papaya to rot quickly and is dangerous if it continues to be consumed. The purpose of this study was to determine the description of contaminant mold contamination in papaya fruit (Carica papaya L).. The type of research used is descriptive. The test samples were 30 papaya samples that had been stored at room temperature for 5 days with physical injuries in the fruit. The growing mold will be microscopically examined using the Lactophenol Cutton Blue dye. Based on the results of research from 30 papaya fruit samples (Carica papaya L) found three types of mold with a percentage of each, namely Aspergillus flavus (60%), Rhizopus oryzae (23.3%) and Aspergillus niger (16.7%). This suggests that papaya fruits left at room temperature in the presence of physical injuries can trigger mold growth.  Keywords : Papaya Fruit, Aspergillus flavus, Rhizopus oryzae, Aspergillus niger 
Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur Mulya Fitrah Juniawan; Dita Artanti; Yuni Gayatri; AINUTAJRIANI AINUTAJRIANI
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15898

Abstract

Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge. Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.
Edukasi Dan Sosialisasi Cuci Tangan Pakai Sabun Di KB – TK Aisyiyah Kureksari Pasca Pandemi Covid-19 Anindita Riesti Retno Arimurti; Ellies Tunjung Sari Maulidiyanti; Fitrotin Azizah; Nur Vita Purwaningsih; Dita Artanti; Yeti Eka Sispita Sari
Empowerment: Jurnal Pengabdian Masyarakat Vol. 2 No. 4 (2023): JULI 2023
Publisher : Pusat Riset Manajemen dan Publikasi Ilmiah Serta Pengembangan Sumber Daya Manusia Sinergi Cendikia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55983/empjcs.v2i4.408

Abstract

The COVID-19 pandemic has changed many aspects of our daily lives, including in the school environment. School is an environment where children learn and interact, making them vulnerable to spreading infectious diseases. Washing hands with soap is a simple tion effective in preventing the spread of viruses and maintaining health in schools. Washing hands with soap is practical in killing viruses and bacteria that stick to hands. Soap has properties that can destroy the fat layer of the virus, so the virus cannot survive. By washing hands with soap regularly, children can reduce the risk of contracting COVID-19 and other infectious diseases. This community court activity consists of three stages: preparation, implementation and evaluation. This community service activity was conducted at KB – TK Aisyiyah 1 Kureksari, Sidoarjo. The method used is in the form of posters and extension demonstrations with movement and music methods. After participating in this activity, students can apply hand washing with soap after activities outside the home or before eating.
DETEKSI EKTOPARASIT DAN ENDOPARASIT PADA KUCING PELIHARAAN (FELIS DOMESTICUS) DI WILAYAH TAMBAKSARI, SURABAYA Anindita Riesti Retno Arimurti; Vella Rohmayani; Dita Artanti; Ruspeni Daesusi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 1 (2023): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i1.3322

Abstract

Domestic Cats (Felis domesticus) are favorite mammals that are mostly kept by humans. One of the obstacles in keeping cats is that they can be infected with ectoparasites and endoparasites. Many ectoparasite and endoparasite infections are zoonotic, so the cat's hygiene and health must be maintained by the owner. This study aims to determine the presence of ectoparasite and endoparasite infections in domestic cats in the Tambaksari area of Surabaya.The sample in this study were 32 cats that were kept in the Tambaksari area, Surabaya. This research was conducted on 32 domestic cats in the Tambaksari area of Surabaya. The examination was divided into 2, namely the examination of ectoparsites by means of which were carried out on the body surface, starting from the head, body, legs, and tail. While the examination of endoparasites by means of examination of feces by flotation method using saturated NaCl. The results of the examination were 21.87% positive for infection by ectoparasites, 78.12% were not infected with ectoparasites. The types of ectoparasites that infect are the Felicola subrostarus and the Ctenocephalides felis. While the results of endoparasite examination, as many as 9.37% were positively infected by endoparasites, 90.63% were not infected with endoparasites. Types of endoparasites found eggs of Toxocara spp. and Ancylostoma spp. So ectoparasites and endoparasites infections in domestic cats in the Tambaksari area of Surabaya are quite low. Cat owners should pay more attention to the cleanliness and health of their domestic cats Keyword: Ectoparasite, Endoparasite, Cat, Felicola subrostarus, Ctenocephalides felis, Toxocara spp., Ancylostoma spp.
Edukasi Deteksi Dini Kanker Serviks Pada Wanita Usia Subur Di Kelurahan Dukuh Sutorejo Ira Purnamasari; Ade Susanty; Diah Ariana; Dita Artanti; Firman Firman
Humanism : Jurnal Pengabdian Masyarakat Vol 4 No 2 (2023): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v4i2.19324

Abstract

Abstrak  Kanker serviks merupakan kanker yang tumbuh pada sel-sel leher rahim yang 95% disebabkan oleh human papilloma virus dan ditularkan melalui hubungan seksual. Kanker serviks merupakan penyakit mematikan bagi wanita. Salah satu penyebab angka kejadian kanker serviks tinggi adalah rendahnya pengetahuan mengenai deteksi dini kanker serviks. Pengabdian ini dilakukan sebagai upaya meningkatkan pengetahuan tentang deteksi dini kanker serviks melalui pemeriksaan IVA di Puskesmas Kalijudan Surabaya. Tim pengabdian memberikan pendidikan kesehatan tentang kanker serviks dan pemeriksaan IVA pada wanita usia subur. Hasil kuesioner pengetahuan responden sebelum diberikan edukasi adalah pengetahuan baik (33,4%), pengetahuan cukup (54,5%), dan pengetahuan kurang (12,1%). Sedangkan, hasil pengetahuan sesudah diberikan edukasi adalah pengetahuan baik (72,7%) dan pengetahuan cukup (27,3%). Setelah kegiatan pengabdian ini, secara signifikan meningkatkan pengetahuan wanita usia subur tentang kanker serviks dan diharapkan dapat meningkatkan capaian program pemeriksaan IVA di Puskesmas Kalijudan Surabaya.  Kata Kunci: deteksi dini, kanker serviks, wanita usia subur
ANALISIS MIKROBIOLOGI PADA JAJANAN PEDAGANG KAKI LIMA DI SEPANJANG JALAN SUTOREJO SURABAYA Ainutajriani Ainutajriani; Dita Artanti; Mulya Fitrah Juniawan
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3559

Abstract

Coliform bacteria are indicators of food contamination that can cause diseases such as diarrhea. The presence of Coliform bacteria in food or beverages indicates poor hygiene and sanitation in food service providers. The purpose of this study was to determine Coliform bacteria in street vendors' snacks along Sutorejo street in Surabaya using the MPN (Most Probable Number) method, ALT (Total Plate Number) and identification of Coliform bacteria with Biochemical tests. This research method uses laboratory experiments. The results showed, of the 12 samples of snacks, there were 4 (33%) samples that met the MPN quality requirements and 8 (67%) samples that did not meet the requirements. Based on the ALT test, 3 (25%) samples met the SNI requirements and 9 (75%) samples did not meet the requirements. Coliform bacteria identification results, there were 6 (50%) positive samples of E.coli bacteria, 2 (17%) positive samples of Klebsiella sp., 1 (8%) positive sample of Enterobacter sp. and 3 (25%) samples were not contaminated by Coliform bacteria. From the results of this study it can be concluded that there are still many snacks contaminated by Coliform bacteria and dominated by E.coli.
Aktivitas Lalat Rumah (Musca domestica) pada Pemberian Ekstrak Daun Pepaya (Carica papaya) daesusi, ruspeni; Anindita Riesti Retno Arimurti; Dita Artanti; Fitrotin Azizah; Novaulia Kinasih; Sispita Sari , yeti Eka
PEDAGO BIOLOGI Vol 12 No 1 (2024): April
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pbjppb.v12i1.22677

Abstract

ABSTRACT House flies (Musca domestica) are vectors or mechanical transmitters of various infectious diseases through their body parts. The use of chemical insecticides to control houseflies has harmful effects on non-target insects, as well as exposure to humans and the environment. The use of insecticides made from natural ingredients is a solution to overcome the use of synthetic chemical insecticides. Papaya (Carica papaya) is a plant that is easy to grow and contains flavonoids, tannins, alkaloids, saponins, papain, calpain, and a number of other secondary metabolic compounds that are anti-mycoorganisms and parasites. This type of experimental research with a post test only group design aims to test the effect of papaya leaf extract on house fly activity. There are 3 treatments, namely P1 (papaya leaf extract), P2 (aquades) and P3 (branded liquid mosquito repellent). Each treatment was repeated 6 times. The research sample was 104 house flies. The results of the Mann Whitney test showed that there was a very significant difference (p<0.01) in the number (presentation) of houseflies that experienced changes in normal activity to abnormal ones between the administration of papaya leaf extract (72%) and the negative control using distilled water (0%). Meanwhile, administering branded liquid mosquito repellent provides 100% of the effect of flies experiencing abnormal activity. Thus, papaya leaf extract has been proven to have potential as a house fly repellent, although the effect is not as great as branded liquid mosquito repellent (p<0.05). Key words: Carica papaya, leaf extract, Musca domestica
Karakterisasi Bakteri pada Urine Suspek Infeksi Saluran Kemih Mahasiswa di Universitas Muhammadiyah Surabaya ainutajriani, ainutajriani; Artanti , Dita; Rohmayani, Vella; Kunsah, Baterun
Camellia : Clinical, Pharmaceutical, Analytical and Pharmacy Community Journal Vol 3 No 2 (2024): Camellia (Clinical, Pharmaceutical, Analytical, and Pharmacy Community Journal)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/cam.v3i2.24765

Abstract

Bakteri yang tumbuh di saluran kemih sering menyebabkan infeksi saluran kemih (ISK), yang merupakan salah satu penyakit menular yang paling umum. Tujuan dari penelitian ini adalah untuk menemukan bakteri yang menyebabkan ISK pada mahasiswa Universitas Muhammadiyah Surabaya. Pengambilan sampel urin dari 20 mahasiswa yang diduga menderita ISK digunakan dalam pendekatan deskriptif. Hasil identifikasi menunjukkan bahwa bakteri gram-negatif, terutama Escherichia coli (75%), adalah penyebab utama ISK. Klebsiella (10%) dan Pseudomonas aeruginosa (5%) juga ditemukan di kasus nosokomial, sementara bakteri gram-positif, seperti Staphylococcus aureus dan Streptococcus sp., masing-masing ditemukan pada 5% kasus. Hasil ini menunjukkan betapa pentingnya pengetahuan tentang bakteri penyebab ISK dan faktor risiko seperti pola hidup dan kebiasaan buruk yang dapat mempengaruhi kejadian ISK.
Aktivitas Lalat Rumah (Musca domestica) pada Pemberian Ekstrak Daun Pepaya (Carica papaya) daesusi, ruspeni; Anindita Riesti Retno Arimurti; Dita Artanti; Fitrotin Azizah; Novaulia Kinasih; Sispita Sari , yeti Eka
PEDAGO BIOLOGI Vol 12 No 1 (2024): April
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pbjppb.v12i1.22677

Abstract

ABSTRACT House flies (Musca domestica) are vectors or mechanical transmitters of various infectious diseases through their body parts. The use of chemical insecticides to control houseflies has harmful effects on non-target insects, as well as exposure to humans and the environment. The use of insecticides made from natural ingredients is a solution to overcome the use of synthetic chemical insecticides. Papaya (Carica papaya) is a plant that is easy to grow and contains flavonoids, tannins, alkaloids, saponins, papain, calpain, and a number of other secondary metabolic compounds that are anti-mycoorganisms and parasites. This type of experimental research with a post test only group design aims to test the effect of papaya leaf extract on house fly activity. There are 3 treatments, namely P1 (papaya leaf extract), P2 (aquades) and P3 (branded liquid mosquito repellent). Each treatment was repeated 6 times. The research sample was 104 house flies. The results of the Mann Whitney test showed that there was a very significant difference (p<0.01) in the number (presentation) of houseflies that experienced changes in normal activity to abnormal ones between the administration of papaya leaf extract (72%) and the negative control using distilled water (0%). Meanwhile, administering branded liquid mosquito repellent provides 100% of the effect of flies experiencing abnormal activity. Thus, papaya leaf extract has been proven to have potential as a house fly repellent, although the effect is not as great as branded liquid mosquito repellent (p<0.05). Key words: Carica papaya, leaf extract, Musca domestica
Karakterisasi Bakteri pada Urine Suspek Infeksi Saluran Kemih Mahasiswa di Universitas Muhammadiyah Surabaya ainutajriani, ainutajriani; Artanti , Dita; Rohmayani, Vella; Kunsah, Baterun
Camellia : Clinical, Pharmaceutical, Analytical and Pharmacy Community Journal Vol 3 No 2 (2024): Camellia (Clinical, Pharmaceutical, Analytical, and Pharmacy Community Journal)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/cam.v3i2.24765

Abstract

Bakteri yang tumbuh di saluran kemih sering menyebabkan infeksi saluran kemih (ISK), yang merupakan salah satu penyakit menular yang paling umum. Tujuan dari penelitian ini adalah untuk menemukan bakteri yang menyebabkan ISK pada mahasiswa Universitas Muhammadiyah Surabaya. Pengambilan sampel urin dari 20 mahasiswa yang diduga menderita ISK digunakan dalam pendekatan deskriptif. Hasil identifikasi menunjukkan bahwa bakteri gram-negatif, terutama Escherichia coli (75%), adalah penyebab utama ISK. Klebsiella (10%) dan Pseudomonas aeruginosa (5%) juga ditemukan di kasus nosokomial, sementara bakteri gram-positif, seperti Staphylococcus aureus dan Streptococcus sp., masing-masing ditemukan pada 5% kasus. Hasil ini menunjukkan betapa pentingnya pengetahuan tentang bakteri penyebab ISK dan faktor risiko seperti pola hidup dan kebiasaan buruk yang dapat mempengaruhi kejadian ISK.