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Uji Antibakteri Perasan Daun Kersen (Muntingia Calabura L) Dalam Berbagai Konsentrasi Terhadap Pertumbuhan Escherichia Coli Secara Invitro Fitrotin Azizah; Lina Listiana; Mulya Fitrah Juniawan; Yatimatus Sholihah
PEDAGO BIOLOGI Vol 10 No 1 (2022)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/pb:jppb.v10i1.14440

Penyakit infeksi merupakan penyakit yang sangat populer di Indonesia sejak lalu, diantaranya infeksi usus (diare). Beberapa bakteri yang dapat menyebabkan diare yaitu Escherichia coli, Vibrio cholera, Shigella sp dan Campylobacter. Pengobatan diare bisa dilakukan dengan pemberian obat-obat kimia dan obat-obat tradisional. Pilihan bahan yang mungkin dapat digunakan sebagai alternatif antibakteri adalah tanaman kersen (Muntingia Calabura L). Penelitian sebelumnya menunjukkan bahwa ekstrak dari daun kersen (Muntingia Calabura L) mampu menghambat pertumbuhan bakteri karena kandungan yang terdapat di dalamnya seperti flavonoid, tanin dan saponin. Sedangkan penelitian ini menggunakan perasan. Penelitian berikut ini bertujuan untuk mengetahui pengaruh perasan daun kersen (Muntingia Calabura L) terhadap pertumbuhan Escherichia coli. Jenis penelitian ini adalah eksperimental. Populasi dalam penelitian ini adalah bakteri Escherichia coli yang diambil dari biakan murni Escherichia coli yang ditanam di media NAS(Nutrient agar slant), dan yang digunakan sebagai sampel adalah bakteri Escherichia coli pada masing-masing konsentrasi yang dilihat pertumbuhannya pada media EMB setelah diinkubasi selama 24 jam. Jumlah pengulangan sampelnya sebanyak 3 kali dan 11 perlakuan yaitu 100%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10% dan 0% (sebagai kontrol). Data pertumbuhan bakteri Escherichia coli diperoleh melalui uji laboratorium. Analisa data statistik menggunakan uji Chi-square dengan taraf signifikan 0,05 untuk menentukan ada tidaknya pengaruh perasan daun kersen (Muntingia calabura L) terhadap pertumbuhan bakteri Escherichia coli. Hasil penelitian menunjukkan bahwa pada konsentrasi 100% dan 90% mampu menghambat pertumbuhan bakteri Escherichia coli, dan sedangkan pada konsentrasi 80% kebawah tidak mampu menghambat pertumbuhan bakteri Escherichia coli.

EDUKASI DAN PELAYANAN KARYAWAN DI UNIVERSITAS MUHAMMADIYAH SURABAYA MELALUI KONSUMSI BAKTERI ASAM LAKTAT UNTUK PENINGKATAN SISTEM IMUN DI COVID-19 Dita Artanti; Nur Vita Purwaningsih; Yeti Eka Sispita Sari; Fitrotin Azizah; Rinza Rahmawati Samsudin; Vella Rohmayani; Anindita Riesti Retno Arimurti; Mulya Fitrah Juniawan
Humanism : Jurnal Pengabdian Masyarakat Vol 3, No 2 (2022): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v3i2.14354

Pada tahun 2020 seluruh dunia sedang mengalami kondisi pandemik akibat infeksi virus. Virus SARS-CoV-2 yang dapat menyebabkan penyakit COVID-19. Kondisi ini mengakibatkan “Panic Buying”, karena semua orang mencoba untuk menghindari penyebaran dan penularan penyakit COVID-19 dengan melakukan berbagai cara, salah satunya dengan mengambil tambahan suplemen seperti vitamin C dan suplemen probiotik. Probiotik merupakan kelompok bakteri asam laktat, jika dikonsumsi dalam jumlah yang cukup dapat memberikan manfaat kesehatan bagi tubuh. Bakteri asam laktat memiliki fungsi sebagai imunomodulator atau meningkatkan sistem kekebalan tubuh sehingga dapat mencegah infeksi penyakit pada hewan dan manusia. Kegiatan edukasi dan pelayanan dilakukan melalui poster dan penyampaian langsung dengan metode door to door. Sehingga karyawan di Universitas Muhammadiyah Surabaya lebih mudah memahami terkait konsumsi bakteri asam laktat untuk peningkatan sistem imun di COVID-19. Para karyawan sangat antusias dengan kegiatan edukasi yang dilakukan, mereka juga menjadi lebih disiplis untuk menghindari penularan dan pencegahan penyakit COVID-19.Kata Kunci: edukasi dan pelayanan karyawan, COVID-19, Bakteri Asam Laktat, dan sistem imun.

Concept Attainment Model (CAM) in Empowering Critical Thinking Skills and Concepts Mastery for High School Students Asy'ari Asy'ari; Wiwi Wikanta; Mulya Fitrah Juniawan
SEJ (Science Education Journal) Vol 6 No 2 (2022): November
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/sej.v6i2.1625

Abstract.Existing human resources must be prepared early. Indeed, national education aims to create intelligent and competitive generations. This research is oriented towards the influence of CAM, which is implemented in the learning process. This research method is quasi-experimental. The research design used a non-equivalent control group design. As a sample is XI grade in SMA Muhammadiyah 7 Surabaya. Data collection techniques through observation and tests, critical thinking skills test sheets and students' concept mastery tests. Descriptively, self-critical thinking skills are 100% completed classically. The results of the t-test have a significance value of ≤ 0.05, which is 0.000. Then for mastery of the concept from the results of the t-test, the significance value is ≤ 0.05, namely with a significance value of 0.000. From the results of this study, it can be concluded that there is an influence of CAM p on students' critical thinking skills and students' mastery of concepts.

The Effect of the Jigsaw Learning Model in Animal Tissue and Body Structure Courses on Student Problem Solving Ability Mulya Fitrah Juniawan; Wiwi Wikanta; Asy'ari Asy'ari
Jurnal Penelitian Pendidikan IPA Vol 9 No 4 (2023): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i4.3129

This study aims to find out how students' problem-solving skills in the subject of animal body tissue and structure use the Jigsaw learning model. This type of research is quantitative research with a sample size of 70 students of the biology education study program. The research sample was taken from students who were normally distributed, had the same characteristics (homogeneous), and had the same average value. The data analysis technique uses simple linear regression. The results showed that there was a significant influence between the Jigsaw cooperative learning model in the animal tissue and body structure course on the problem-solving abilities of students in the biology education study program with a significance value of 0.000 <0.05, so that the jigsaw learning model can help students improve problem-solving skills

Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur Mulya Fitrah Juniawan; Dita Artanti; Yuni Gayatri; AINUTAJRIANI AINUTAJRIANI
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15898

Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge.Â Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.

ANALISIS MIKROBIOLOGI PADA JAJANAN PEDAGANG KAKI LIMA DI SEPANJANG JALAN SUTOREJO SURABAYA Ainutajriani Ainutajriani; Dita Artanti; Mulya Fitrah Juniawan
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3559

Coliform bacteria are indicators of food contamination that can cause diseases such as diarrhea. The presence of Coliform bacteria in food or beverages indicates poor hygiene and sanitation in food service providers. The purpose of this study was to determine Coliform bacteria in street vendors' snacks along Sutorejo street in Surabaya using the MPN (Most Probable Number) method, ALT (Total Plate Number) and identification of Coliform bacteria with Biochemical tests. This research method uses laboratory experiments. The results showed, of the 12 samples of snacks, there were 4 (33%) samples that met the MPN quality requirements and 8 (67%) samples that did not meet the requirements. Based on the ALT test, 3 (25%) samples met the SNI requirements and 9 (75%) samples did not meet the requirements. Coliform bacteria identification results, there were 6 (50%) positive samples of E.coli bacteria, 2 (17%) positive samples of Klebsiella sp., 1 (8%) positive sample of Enterobacter sp. and 3 (25%) samples were not contaminated by Coliform bacteria. From the results of this study it can be concluded that there are still many snacks contaminated by Coliform bacteria and dominated by E.coli.

Study of 21st-Century Skill Improvement for Prospective Biology Teacher Students Through the Lesson Study (LS) Model Wikanta, Wiwi; Asy'ari, Asy'ari; Juniawan, Mulya Fitrah
Jurnal Pendidikan Biologi Vol 12, No 3 (2023): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v12i3.48507

Education in Indonesia faces the problem of preparing human resources to face the world of work in the 21st Century Era. This study aims to describe the results of a Lesson Study on Improving 21st Century Skills for Biology Education Teacher Candidates at FKIP UMSurabaya. This research is a case study with a descriptive method. The research was conducted on the Department of Biology Education students for the 2019 Academic Year at FKIP UMSurabaya. Data were collected by documentation, test, and observation methods from 2 Lesson study cycles. Data were analyzed descriptively by calculating the percentage of students with HOTS, communication, and collaboration skill levels expressed in four categories. The results of the study showed: 1) the level of HOTS in the Very High Category in Cycles I and II was the same, namely 50% of students; 2) the level of Communication skills in the Very Competent Category in Cycle I was 26% of students and Cycle II was 36% of students; 3) the level of collaborative skills with the Very Competent Category in Cycle I was 25% of students, and Cycle II was 37% of students. The conclusion of this study shows that during Lesson Study, as many as two cycles of Biology Education Teacher Candidates at FKIP UMSurabaya have not shown an increase in HOTS. Meanwhile, his communication and collaboration skills have improved. The sustainability of the Lesson Study needs to be continued by building a learning community between lecturers of other subjects and partner teachers in schools.

Pemanfaatan buah campolay (Pouteria campechiana) dalam meningkatkan kualitas wajit cililin Wikanta, Wiwi; Juniawan, Mulya Fitrah; Mardiyah, Siti
ARGIPA (Arsip Gizi dan Pangan) Vol 10 No 1 (2025)
Publisher : UHAMKA PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/argipa.v10i1.18653

Wajit Cililin is one of the traditional foods typical of the Cililin Region in West Java. The making of wajit can be varied by adding fruits, such as Campolay Fruit. The use of Campolay Fruit (Pouteria campechiana) in making wajit has never been done before. The purpose of this study was to determine the organoleptic value and nutritional content of Wajit Cililin with the addition of Campolay Fruit (Pouteria campechiana). The study was conducted using an experimental method at the East Java Health Laboratory (Labkesda) and the Medical Laboratory Technology Lab, Muhammadiyah University of Surabaya. Organoleptic data were collected from 20 panelist selected by accidental sampling consisting of men and women aged 17-65 years. Organoleptic and nutritional data were analyzed descriptively. The results showed that panelist really liked the organoleptic in treatment III (wajit with the addition of 200 g Campolay fruit) with an average value of 3.31 (very much like). The nutritional content showed an increase in the addition of 200 g of Campolay Fruit with nutritional values including Calories, Carbohydrates, Fat, Protein, Calcium, β-Carotene, and Vitamin C respectively, namely 262.47 Kcal, 50.62 mg, 5.15 mg, 3.41 mg, 642.85 mg, 1,600 ppm, and 17.5 mg. The results of this study can be concluded that the use of Campolay Fruit (Pouteria campechiana) can improve the quality of Wajit Cililin, both in terms of organoleptic value and nutritional content.

Identifikasi Jamur Penyebab Infeksi Kulit Pada Sela Jari Kaki Pekerja Migran Indonesia (PMII) di Malaysia Azizah, Fitrotin; Artanti, Dita; Arimurti, Anindita Riesti Retno; Sari, Yety Eka Sispita; Ali, Nur Azeera Mohd; Juniawan, Mulya Fitrah; Daesusi, Ruspeni
Jurnal Pengabdian Meambo Vol. 4 No. 2 (2025): Jurnal Pengabdian Kepada Masyarakat MEAMBO
Publisher : PROMISE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56742/jpm.v4i2.146

Pekerja Migran Indonesia yang bekerja pada sektor kontruksi, perkebunan dan perindustrian merupakan kelompok rentan terhadap infeksi jamur kulit. Kondisi kaki yang lembap, kebersihan diri yang kurang optimal serta penggunaan alas kaki yang tidak memadai menjadi faktor utama terhadap munculnya infeksi jamur pada sela jari kaki. Tujuan dari kegiatan pengabdian ini yaitu mengetahui jenis jamur penyebab infeksi kulit di sela jari kaki, memberikan edukasi tentang kebersihan kulit dan pencegahan terhadap infeksi jamur, serta rekomendasi penanganan awal untuk mengurangi angka kejadian infeksi jamur. Pengabdian ini dilakukan dari bulan Agustus sampai dengan September 2024. Metode yang dilakukan pada kegiatan pengabdian ini yaitu metode koordinasi dengan penanggung jawab setempat, penyampaian rencana kegiatan secara langsung kepada peserta, serta pelaksaan pemeriksaan swab sela jari kaki. Hasil yang diperoleh yaitu dari 26 swab sela jari kaki PMI, yang terinfeksi yeast sebanyak 16 orang (61.5%), Aspergillus niger 7 orang (27%), Aspergillus fumigatus 2 orang (7.7%), Mallasezia furfur 1 orang (3.8%). Kesimpulan dari hasil kegiatan pengabdian ini yaitu kondisi lingkungan yang lembap dan hangat, serta didukung dari jenis pekerjaan dari para PMI yang bervariasi mengharuskan untuk selalu menggunakan penutup kaki, menjadikan tingkat infeksi jamur kulit tinggi. Langkah pencegahan yang efektif terhadap infeksi jamur kulit kaki dengan edukasi mengenai kebersihan kaki dan kontrol lingkungan

Isolasi Dan Karakterisasi Bakteri Termofilik Dari Oil Sludge Asal Kalimantan Timur Juniawan, Mulya Fitrah; Artanti, Dita; Gayatri, Yuni; AINUTAJRIANI, AINUTAJRIANI
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.15898

Oil sludge is a product of petroleum mining activities and causes environmental pollution. However, oil sludge, including hazardous and toxic waste materials (B3), has been less effective in reducing oil sludge pollution. Thus, the researcher solved the issue by using hydrocarbonoclastic. It is necessary to use hydrocarbonoclastic bacteria isolated directly from their habitat (indigenous bacteria) as hydrocarbon degrading agents. Therefore, this study aimed to isolate and identify indigenous thermophilic bacteria from East Kalimantan Oil sludge. This study is an observational study that is analyzed descriptively. Procedure for isolation and identification of thermophilic bacteria from oil sludge grow on Synthetic Mineral Water media (SMW) with and without an autoclave. The Microbact Identification System Kit GNB 24E was used to characterize colonies macroscopically, microscopically, Gram staining, physiological tests (catalase, coagulase, and motility tests), and biochemically. The bacteria that were successfully isolated were later identified with Microbact Software and Bergey's book Manual of Determinative Bacteriology Ninth Edition. The results of the isolation and identification of thermophilic Indigenous bacteria from Oil Sludge Kalimantan Timur found Pseudomonas aeruginosa species with a similarity accuracy of 98.33%. The identified bacterial isolates can later be used as bioremediation agents on soils polluted with oil sludge.Â Keywords : Indigenous bacteria, Oil Sludge, Pseudomonas aeruginosa.

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