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All Journal Jurnal Kedokteran Syiah Kuala Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana INDONESIAN JOURNAL OF PHARMACY Jurnal Surya Medika Warta Pengabdian Jurnal Ilmu Kefarmasian Indonesia Harmony : Jurnal Pembelajaran IPS dan PKN Jurnal Pengabdian Masyarakat Asia Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati
Sudjarwo Sudjarwo
Faculty Of Pharmacy, Airlangga University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

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THE OPTIMATION OF SPECTROFLOUROMETRIC METHOD FOR DETERMINATION OF MALONIC DIALDEHYDE IN HUMAN SPERM Sudjarwo .
Indonesian Journal of Pharmacy Vol 12 No 3, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.88 KB) | DOI: 10.14499/indonesianjpharm0iss0pp152-158

Abstract

Optimation of a method is an obligatory step in order one to obtain a valid and accountable data, especially when one deal with biological samples. Biological samples usually contain minute substances which sometime elicit great errors when someone try to determine its concentration. Therefore, in order to reduce those errors, optimation of a method is mandatory to be carried out. The aim of this experiment was to determine malonic dialdehyde (MDA), a product of lipid peroxidation. The optimation carried out in this experiment include the selection of wavelength of maximum absorbance, the amount of sodium thiobarbiturate, pH, the incubation period, standard curve linearity, limit of detection and quantification, accuracy and precision. The result showed that the optimum conditions of spectrofluorometric method of MDA were as follow: the excitation and emission wavelengths was 522 and 575 nm, respectively, pH of 1 and incubation period of 135 minutes. These conditions yielded correlation coefficient of standard curve of 0.9993, limit detection and quantification of 3.5146 x 10-4 mg/ml and 1.0544 x 10-3 mg/ml, respectively, an average recovery of 85.01 % and variation coefficient of 1.84 %.Key words: optimation, spectrofluorometric, sperm, malonic dialdehyde, MDA
Co-Authors Achmad Toto Poernomo Afif Nurul Hidayati Angerina N Ashon Sa’adi Asri Darmawati Aucky Hinting Budi Utomo Dasrul Dasrul Diah Mira Indaramaya Didik Rio Pambudi Doddy M. Soebady Dwi Murtiastutik Emma Hidayati Sasmito Hanifa Rahma Putri Hardjopranjot Hardjopranjot Irvan Ipandi Isnaeni Kurniawan, Hanif Lidya Tumewu Mahaputra L Marcellino Rudyanto Muhammad Hakim Rafiga Muhammad Muslich Noor Cholies Zaini Pepy Dwi Endraswari, Pepy Dwi Poedjiarti S Purwitasari, Neny Rahmadewi Rice Disi Oktarina Risma Margaretha Sinaga Sawitri Suciati Suciati Sukarjati Sukarjati Sukarjati Susie Amilah Tutiek Purwanti Wiwied Ekasari Yanu Andhiarto ZAINAL ABIDIN