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All Journal Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat JFMR (Journal of Fisheries and Marine Research) Omni-Akuatika Journal of Aquaculture and Fish Health
Fiddy Semba Prasetiya
Departemen Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Engineering Environmental Science Languange, Linguistic, Communication & Media Public Health

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Study of Symbiont Bacteria of Acropora digitifera Coral From Ciletuh Bay, Sukabumi by Using Culture and Molecular Approach Nurfitri Nurfitri; Fiddy Semba Prasetiya; Indah Riyantini; Yuniar Mulyani; Sulastri Arsad; Mochamad Untung Kurnia Agung
Journal Omni-Akuatika Vol 15, No 2 (2019): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2019.15.2.748

Abstract

Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were used to determine the bacterial species in the samples. A total of six pure isolates were identified based on morphological observation and the molecular DNA characteristics from three of them were identified with 16S rRNA gene sequences. The identification using 16S rRNA gene showed that the isolate ACD.P4.PH7.P had a close relationship with the BF strain and zb strain of Bacillus flexus (acc number MH569560.1) with a similarity of 85.44 %. While ACD.P4.PH9.P isolate has a close relationship with Bacillus sp. c234 (acc number FJ950647.1) with a similarity of 98.50 %. Additionally, ACD.P4.PH9.K isolates closely related to Bacillus sp. of strain 6RM1 (acc number MK134607.1) with a similarity of 94.78 %. This study also revealed that both microscopic identifications by morphological traits and molecular approach using 16S rRNA gene can be used in bacterial diversity assessment.
SOSIALISASI PEMANFAATAN SENYAWA BIOAKTIF DARI MIKROALGA DIATOM DALAM PRAKTIK BUDIDAYA PERIKANAN BERKELANJUTAN DI KABUPATEN INDRAMAYU Sunarto Sunarto; Fiddy Semba Prasetiya; Eri Bachtiar
Dharmakarya Vol 9, No 2 (2020): Juni, 2020
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v9i2.23858

Abstract

Mikroalga diatom dianggap sebagai organisme hidup yang memiliki banyak manfaat dan telah menarik banyak perhatian dari peneliti-peneliti dunia. Dalam beberapa tahun ini, diatom juga telah digunakan sebagai bahan nutrasetikal dan sumber obat dalam industri farmasi. Penduduk lokal di Desa Pabean Udik, Kabupaten Indramayu, sebagian besar memiliki profesi sebagai nelayan pembudidaya, dimana produksi perikanan budidaya berkontribusi cukup besar pada pendapatan daerah. Namun, salah satu kendala yang dialami oleh pembudidaya adalah adanya serangan penyakit yang disebabkan oleh bakteri patogen yang seringkali diatasi dengan penggunaan antibiotik sintetik yang tidak ramah lingkungan. Oleh karena itu, dalam penelitian ini, kami melakukan sosialisasi terhadap peningkatan kesadaran masyarakat lokal di Desa Pabean Udik tentang potensi diatom sebagai produk alami dengan multi-manfaat, terutama sebagai agen antibakteri alami dalam praktik budidaya perikanan laut. Dalam studi ini, pendidikan berbasis masyarakat dengan beberapa kegiatan seperti ceramah, diskusi, video interaktif dan sesi doorprize dilakukan. Selain itu, kuesioner pre-test dan post-test dilakukan sebagai alat untuk mengidentifikasi kesadaran peserta dan untuk mengukur indikator tujuan. Hasil survey yang diperoleh menunjukkan peningkatan pemahaman peserta hingga 20%. Kami mengamati bahwa selama diskusi peserta juga menunjukkan kesediaan mereka untuk meningkatkan pengetahuan mereka tentang potensi pemanfaatan diatom untuk meminimalkan risiko infeksi patogen. Oleh karena itu, hasil ini menunjukkan bahwa penelitian ini berhasil meningkatkan pemahaman masyarakat lokal tentang potensi manfaat diatom dan meningkatkan kesadaran masyarakat untuk menggunakan diatom untuk banyak tujuan, terutama dalam menggantikan obat antimikroba yang tidak ramah lingkungan.
KOMUNITAS MIKROALGA PERIFITON PADA SUBSTRAT BERBEDA DAN PERANNYA SEBAGAI BIONDIKATOR PERAIRAN (Microalga Peryphyton Community on Different Substrates and Its Role as Aquatic Environmental Bioindicator) Sulastri Arsad; Nur ALiya Zsalzsabil; Fiddy Semba Prasetiya; Ikha Safitri; Dhira Kurniawan Saputra; Muhammad Musa
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 15, No 1 (2019): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.08 KB) | DOI: 10.14710/ijfst.15.1.73-79

Abstract

Mikroalga merupakan salah satu komponen penyusun perifiton yang hidupnya melekat pada substrat, baik substrat alami maupun substrat buatan. Perifiton memiliki peran salah satunya sebagai bioindikator. Penelitian ini bertujuan untuk menganalisis komunitas mikroalga perifiton pada substrat berbeda yaitu substrat alami (batu) dan substrat buatan (kaca objek glass) serta untuk menganalisis status mutu perairan berdasarkan pendekatan perifiton. Lokasi penelitian yaitu perairan Ranu Pakis Lumajang dengan 3 stasiun pengambilan sampel secara purposive sampling. Metode penelitian adalah survei dan dilakukan pada musim barat tahun 2019 dengan waktu pengambilan sampel setiap dua minggu sekali selama enam minggu. Parameter yang dianalisis meliputi identifikasi mikroalga perifiton, penghitungan kelimpahan, indeks biologi, dan pengukuran kualitas air. Data yang diperoleh dianalisis secara statistik menggunakan one way ANOVA dan regresi linear berganda menggunakan Ms. Excel dan SPSS 23.0. Hasil penelitian menunjukkan bahwa mikroalga perifiton yang ditemukan berasal dari empat divisi yaitu Chrysophyta (47%), Chlorophyta (27%), Cyanophyta (26%), dan Pyrrophyta (0,2%). Secara keseluruhan kelimpahan yang diperoleh berkisar antara 5.567-34.841 sel/cm2 (substrat alami) dan 14.367-42.563 sel/cm2 (substrat buatan). Indeks keanekaragaman di perairan Ranu Pakis tergolong tinggi sehingga perairan tergolong bersih (stabil), kecuali pada stasiun 3 tergolong tercemar ringan (kurang stabil). Akan tetapi, tidak ada dominansi pada seluruh stasiun tersebut. Hasil pengukuran parameter kualitas air menunjukkan bahwa secara umum nilai kualitas air masih berada di bawah ambang baku mutu yang ditetapkan oleh pemerintah PP No.82 Tahun 2001 untuk kegiatan perikanan kelas III. Komponen kualitas air seperti karbondioksida, kecerahan, nitrat, orthofosfat, dan suhu secara simultan berpengaruh terhadap kelimpahan mikroalga sebesar 39,7%.Periphyton microalgae  live by attaching to the substrate, both natural and artificial substrate. Perifiton plays role as an aquatic environment bioindicator. This study aimed to analyze periphyton microalgae community in different substrate, natural substrate (stone) and artificial substrate (objeck glass). Furthermore, the microalgae periphyton are used to analyze the environmental status quality. Survey method was used and sampling location is in three site of the Ranu Pakis environment by purposive sampling. Research was carried out in 2019 every two week during six weeks on west season. Identification of periphyton microalgae, density counting, biological indexes, and water quality measurements was taken during the research. All data was analyzed statistically by using one way ANOVA and multiple linear regression by using Ms. Excel and SPSS 23.0. The results depict periphyton microalgae consists of Chrysophyta (47%), Chlorophyta (27%), Cyanophyta (26%), and Pyrrophyta (0,2%). The density of microalgae periphyton was ranging from 5.567 to 34.841 sel.cm-2 (natural substrate) and 14.367 to 42.563 sel.cm-2 (artificial substrate). Biological index indicates that Ranu Pakis environment was belonging to stable (unpolluted) in site 1 and 2, while 3 was belonging to less stable (less polluted). However, there was no domination in all sites. Water quality values provide information that in general it still under of quality standards determined by PP No. 82 year 2001 for fisheries activites class III. Water quality components  including carbondioxide, transparency, nitrate, orthophosphate, and temperature simultantly influence the microalgae density of 39,7%. 
A BASELINE STUDY ON MACROZOOBENTHOS ASSEMBLAGES IN PASIR ISLAND Fiddy Semba Prasetiya; Yusuf Arief Nurrahman; Sheila Zalessa; Sri Astuty; Indah Riyantini; Ibnu Faizal
JFMR (Journal of Fisheries and Marine Research) Vol 4, No 2 (2020): JFMR VOL 4. NO.2
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.02.7

Abstract

Biodiversity of macrozoobenthos in Pasir Island, Brebes, is under-explored. On the other hand, the baseline information regarding macrozoobenthos is essentially required for better future environmental management. This research aims to analyze macrozoobenthos community structure in the Pasir Island that has not been documented. Surveys were carried out in the rainy season. In this study, a total of 10 sampling locations were classified into two different groups that represent “control” and “disturbed” station. The main structural parameters of the macrozoobenthos identified at each station were specific richness (S), abundance (number of individuals m-2, N) and the Pielou’s index (J). Additionally, the pairwise comparison between groups was conducted by using analysis of similarity (ANOSIM) and the similarity percentage (SIMPER) to obtain the description of community structure. A total of 1054 specimens were counted from the “control” and “disturbed” stations. These specimens belonged to five animal classes, namely, Bivalvia, Gastropoda, Malacostraca, Cephalopoda and Echinoidea. ANOSIM and SIMPER analysis demonstrated that a significant difference was found between “control“ and  “disturbed“ stations with the percentage of dissimilarity value of 88.82%. In addition, environmental variables such pH, salinity and type of substrates contributed significantly to this difference. This result provides insight regarding macrozoobenthos diversity and ecological information that may contribute to further conservation management in the Pasir Island, Indonesia.
A Survey of Macrozoobenthos Assemblages in a Tropical Mangrove Estuary in Brebes, Java Island Sheila Zallesa; Indah Riyantini; Sri Astuty; Yusuf Arief Nurrahman; Ibnu Faizal; Sulastri Arsad; Marine K Martasuganda; Fiddy Semba Prasetiya
Journal Omni-Akuatika Vol 16, No 1 (2020): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.1.754

Abstract

The mangrove forest in Brebes is one of the mangrove areas that were degraded due to abrasion and excessive logging in the North Coast of Java Island, Indonesia. This research aimed to analyze macrozoobenthos community structure in the mangrove forest that has not been documented. Surveys were carried out in the rainy season. In this study, a total of 10 sampling locations were divided into two different groups that represent “control” (station) and “disturbed” sites. The main structural parameters of the macrozoobenthos identified at each station were specific richness S (number of species), abundance N (number of individuals.m-2), the Shannon-Wiener index N1. A total of 346 specimens were counted from the two different stations (“control” and “disturbed”). These specimens were belonged to five animal classes, namely, Bivalvia, Gastropod, Malacostraca, Polychaeta, Cephalopoda. Pairwise comparison of the site groups with one-way analysis of similarity (ANOSIM) was not significant for between “control” and “disturbed” groups (p=0.062), where the average dissimilarity between the two stations was 88.42%. This result provides macrozoobenthos diversity and ecological information that may contribute to further conservation management in the mangrove forest in Brebes, Indonesia.
In Vivo Test of Rhizophora mucronata Mangrove Extract From Pangandaran Coast Towards Nile Tilapia Oreochromis niloticus infected by Vibrio harveyi Yeni Mulyani; Kiki Haetami; Lesta Krismawati Baeha; Sulastri Arsad; Fiddy Semba Prasetiya
Journal of Aquaculture and Fish Health Vol. 9 No. 2 (2020): JAFH Vol. 9 No. 2 June 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v9i2.16211

Abstract

This study aims to determine the effectiveness of Rhizophora Mucronata’s bark extract in curing Vibrio harveyi infection in nile tilapia fish by challenge testing (in vivo test). It was conducted in October 2018-May 2019 at the Central Laboratory of Universitas Padjadjaran and Building-4 of Aquaculture and Hatchery Laboratory in the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This research used microdilution method to determine the Minimum Inhibitory Concentration (MIC) value of R.mucronata’s bark extracts and laboratory experimental method with a Completely Randomized Design (CRD) model for challenge testing (in vivo) which consisted of 5 treatments and 3 replicates where the given treatment varied in concentrations. These various concentrations that were given started from A (0 ppm / control), B (16 ppm), C (32 ppm), D (48 ppm), and E (64 ppm). The observed parameters were inhibitory antibacterial activity for MIC and survival rate, as well as clinical symptoms and water quality for the challenge test. Survival Rate data of nile tilapia seedlings was analyzed by regression analysis. The observation results of MIC, MBC values, clinical symptoms and water quality were analyzed descriptively. The results showed the Minimum Inhibitory Concentration value of R.mucronata bark extract at a concentration of 6,250 ppm while its Minimum Bacteriocidal Concentration value at 50,000 ppm. The fish tolerance test results towards the extract (LC50 test) is 64 ppm. Differences in treatment produced results that have significant effects on survival rate. The use of Rhizophora Mucronata bark extract at a dose of 64 ppm resulted in the highest survival rate of Nile tilapia fish as high as 76.66%.
Co-Authors Eri Bachtiar Ibnu Faizal Ikha Safitri Indah Riyantini Indah Riyantini Indah Riyantini Kiki Haetami Lesta Krismawati Baeha Marine K Martasuganda Mochamad Untung Kurnia Agung Muhammad Musa Nur ALiya Zsalzsabil Nurfitri Nurfitri Saputra, Dhira Kurniawan Sheila Zalessa Sheila Zallesa Sri Astuty Sri Astuty Sulastri Arsad Sulastri Arsad sunarto sunarto Yeni Mulyani Yuniar Mulyani Yusuf Arief Nurrahman