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BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES Gunawan Pasaribu; Wasrin Syafii; Latifah K. Darusman
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.1.32-49

Raru (Shoreabalanocarpoides Sym) signifies one of the tree species that grows widespread in Sumatra Island. Its bark portion is commonly used by local villagers as additional ingredient mixed to nira (sugar palm juice). This addition is intended to make the juice more durable and also to enrich its taste after the juice is previously fermented to become traditional toddy beverage or the so-call “tuak”. Local villagers believe that raru bark can reduce the level of blood sugar. As the relevance, the research was conducted to confirm that the extract from raru bark could afford its biological activities to inhibit alpha-glucosidase enzyme through its characterization, quantification, and isolation of its boactive compound. The extraction was performed using two methods (i.e.reflux and maceration techniques). Result revealed that the bark extract obtained from both techniques contained polyphenol compounds: flavonoid, saponin and tannin. Further, raru-bark extract from the reflux and maceration techniques could inhibit the action of alpha glucosidase enzymes on carbohydrate substrate ( i.e. p -nitrophenil-α-D-glucopyranose), at respectively 90.67% and 97.33%. Meanwhile, the inhibition activities afforded by the patented drug as a control (i.e. glucobay) equaled to 97.05%. Assesment using UV-VIS spectroscopy, showed that the maximum spectrum of bioactive compound in the extract was at the wave length of 288.6 nm. Scrutiny using FTIR spectroscopy could identif y the presence of aromatic groups in the compound, containing -OH, C-H, C=C, C-O and C-H bond types. Analysis using GC-MS exhibited that the compound had molecular weight of 390 with molecular structure as C20H22O8. Ultimately, data analysis scrutiny with the aid of NMR judged the most plausible compound as bioactive was 4-Glucosyl-3, 4’, 5-trihydroxystilbene.

ANALISIS SENYAWA KIMIA Dryobalanops aromatica Gunawan Pasaribu; Gusmailina; Sri Komarayati; Zulnely; Erik Dahlian
Jurnal Penelitian Hasil Hutan Vol. 32 No. 1 (2014): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2014.32.1.21-26

Dryobalanops aromatica is a woody plant that produces non timber forest product such oil and crystal. Thechemical composition information is important for oil utilization and specific marker of the material. Traditionally, oilquality was grouped based on its colour. The whiter the colour, the better the quality. This paper examines the chemicalcontent of four oil groups and two kinds of crystal. The examination was carried out in Gas Chromatography MassSpectrometry (GCMS). The results show that the oil of Dryobalanops aromatica 1 contains borneol up to 26.02%, D. aromatica 2 contains 13.95%, D. aromatica 3 contains 24.03%, and D. aromatica 4 contains 1.09% in longiborneol form. Meanwhile, crystal 1 contains 92.70% of borneol and crystal 2 contains 90.73%. Borneol compoundcan act as chemical marker of Dryobalanops aromatica.

AKTIFITAS ANTIOKSIDAN DAN ANTIKOAGULASI RESIN JERNANG Totok K. Waluyo; Gunawan Pasaribu
Jurnal Penelitian Hasil Hutan Vol. 31 No. 4 (2013): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2013.31.4.306-315

Dragon's blood is essentially a red-colored resin secreted by the fruits of rattan species. The dragon's blood originated from Indonesia which becomes widespread in international market is indigenous from Daemonorops spp. The dragon's blood has been popularly used as traditional medicines. To confirm such dragon's blood efficacy, an assesment was already conducted regarding the phytochemical screening, antioxidant activity, and anticoagulant activity indicatively afforded by the dragon's blood resin produced by three rattan species, ie. Daemonorops longipes Mart, Daemonorops draco BL, and Daemonorops melanochaetes BL. Phytochemical screening aimed to identify the kinds of chemical compounds inside the dragon's blood resin; antiæcidant tests used DPPH (2,2-diphenyl-1-picrylhydrazyl); and anticoagulation tests proceed in-vitro using rabbit blood. Results revealed that the dragon's blood from those three species, exctracted using polar (methanol) and semi-polar (ethyl acetate) solvents, contained chemical compounds which are already renowed for medicinal efficacy and potent antinccidant, e.g. flavonoids, triterpenoids, and tannin. The greatest antioccidant potency was imparted by dragon's blood from Daemonorops longipes Mart, as indicated by its lowest IC value (71.89U+3,89 mgl.). The ethyl acetate dragon's blood extract, rather than promoting anticoagulat action on the rabbit-blood, in fact induced the blood coagulation, whereby the extract from Daemonorops longipes Mart performed the most effectively (shortest in coagulation time).

PENGARUH PENAMBAHAN NATRIUM BISULFIT DAN PENCUCIAN ETANOL BERTINGKAT TERHADAP KUALITAS TEPUNG PORANG (Amorphophallus muelleri Blume) Gunawan Pasaribu; Totok K. Waluyo; Novitri Hastuti; Gustan Pari; Emma Sahara
Jurnal Penelitian Hasil Hutan Vol. 34 No. 3 (2016): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.3.241-248

Porang (Amorphophallus muelleri Blume) is an alternative food sources from forest. Porang grows under forest canopy and potentially developed to improve food security. Naturally harvested porang contains high oxalate and less glucomannan. This paper observes possible quality improvement of porang flour in term of whiteness and glucomannan content. Porang collected from Nganjuk, East Java was quality tested and mixed with natrium bisulfite then rinse in ethanol repetitively. Results showed that the addition of sodium bisulfite improved the whiteness of porang flour for about 6.59%. Ethanol dehydration proces was able to improve glucomannan content from 12.86% to 38.11%. Fe and Ca content of mixed porang flour showed no significant difference. Porang flour from Nganjuk contained of 1,6-AnhydroBeta-D-Glucopyranose; 1,2,3,4-Cyclopentanetetrol,(1.alpha., 2.beta., 3.beta., 4.alpha.); cyclopropyl carbinol; aceticacid(CAS)ethylicacid; and hexadecanoic acid.

ANALISIS KOMPONEN KIMIA BEBERAPA KUALITAS GAHARU DENGAN KROMATOGRAFI GAS SPEKTROMETRI MASSA Gunawan Pasaribu; Totok K. Waluyo; Gustan Pari
Jurnal Penelitian Hasil Hutan Vol. 31 No. 3 (2013): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2013.31.3.181-185

This paper presents the resin content and the chemical composition of some of agarwood quality using gaschromatography mass spectrometry. The quality of agarwood that tested are kemedangan C, teri C, kacangan C and superAB. The results showed that the extracts yield of various solvent of agarwood are decreasing from super AB towardkacangan C, teri C, and kemedangan C. The agarwood samples contain furan compounds and ester aromatic groups thatresponsible for agarwood's nice scent. This research showed that traditional agarwood quality classification were notobjectives.

KUALITAS LILIN AROMATERAPI DAN SABUN BERBAHAN MINYAK Dryobalanops aromatica Gunawan Pasaribu; Gusmailina; R. Esa Pangersa G
Jurnal Penelitian Hasil Hutan Vol. 34 No. 2 (2016): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2016.34.2.101-110

The objective of this study was to determine the quality of aromatherapy candles and soaps that produced by the addition of Dryobalanops aromatica oil. Aromatherapy candles are made in several formulation variations, and then tested on 30 respondents to know the preference for candles. Parameters measured are prefereed for before candle burned, after burned and the perceived effects of aromatherapy. The soap is made in different formulations by adding Dryobalanops aromatica oil at 2, 4 and 6%, then tested the physico chemical properties. Results show that there are significant differencesof respondent preference between formulations before the candle is burned and formula with the lowest concentration of dryobalanop oil was most preferred. It does not indicate any preference difference between formulations after the candle is burned. However, the trend indicated that candle with medium oil concentration is more accepted. There were no significant differences in aromatherapical effects on various formulas. Aromatherapy candles formulation with the lowest concentration of dryobalanops oil is the most acceptable formulation that gives a positive effect on many respondents. Furthermore, the physico-chemical propertiesof the soap showed that water content and amountof fatty acids are below the standard limit, but the value of free fatty acid and saponified lipid levels could meet the ISO standards. Value of qualitative minerals content is negative. It was found that borneol compound as the Dryobalanops aromatica identifier in the form of endo borneol. The other encountered chemical compounds are caryophylene, alpha pinene, alpha humulene, alpha terpineol and dl-Limonene.

SIFAT ANATOMI, SIFAT FISIS DAN MEKANIS PADA KAYU KEMENYAN TOBA (Styrax sumatrana) DAN KEMENYAN BULU (Styrax paralleloneurus) Gunawan Pasaribu; Jasni; Ratih Damayanti; Santiyo Wibowo
Jurnal Penelitian Hasil Hutan Vol. 31 No. 2 (2013): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2013.31.2.161-169

Gum benzoin (Styrax sp.) is a tree producing resins that have bigh economic value originated from North Sumatra Province. The unproductive trees (less resin production) are harvested by the community for firewood. This paper presents characteristics difference of two types of Kemenyan toba (Styrax sumatrana) and Kemenyan bulu (Styrax paralleloncurus) in anatomical, phisical and mechanical properties in order to use properly. The results showed that kemenyan toba wood and kemenyan bulu are unique anatomical properties. The quality of fiber as a raw material for pulp is categorized into first quality. Based on their spesific gravity, the wood were clasified as medium beavy, with stabile shrinkage. Both wood are suitable for non structural construction, furniture and bandicraft.

AKTIVITAS ANTIJAMUR, ANTIBAKTERI DAN PENYEMBUHAN LUKA EKSTRAK RESIN JERNANG Totok Kartono Waluyo; Gunawan Pasaribu
Jurnal Penelitian Hasil Hutan Vol. 33 No. 4 (2015): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2015.33.4.377-385

“Jernang” or known as dragon’s blood is one of high value non-wood forest products originated from Indonesian forest. Dragon’s blood which is a red colored resin secreted from rattan’s fruits has been utilised traditionally. This paper determines the antibacterial and antifungal capability againts bacteria (Basilus subtilis and Staphylococcus aureus) and fungus (Candida albicans and Aspergillus flavus). The antibacterial and antifungal capability was determined based on the in vivo wound healing test on rabbit. The result shows that in general, “jernang” provides antibacterial and antifungal capacity against bacteria and fungus. The ethyl acetate jernang extract which were effective as antimicrobial to heal the wound.

KERAGAMAN KOMPONEN KIMIA GAHARU PADA KELAS SUPER DAN KEMEDANGAN Gunawan Pasaribu; Totok K.Waluyo; Gustan Pari
Jurnal Penelitian Hasil Hutan Vol. 33 No. 3 (2015): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2015.33.3.247-252

This paper presents to the resin content and the chemical composition in agarwood with several quality using gas chromatography mass spectrometry. The agarwood qualities as tested were those super and kemedangan classes originated from Bangka, Papua and Assosiation of Indosnesia's Agarwood Exportry Enterprise (Asgarin). Results revealed the resin yield of super quality agarwood was higher than that of kemedangan-quality counterpart. Agarwood with high resin content was regarded a high (super) quality, and its chemical composition was predominantly chromone and gamma gurjunene. On the contrary, agarwood with low quality (kemedangan) chemicallyy comprised 2.5 furandione, 3-dodecenyl and agarospirol. Further super-quality agarwood chemically contained more sesquiterpene than kemedangan-quality. Sesquiterpene and chromone compound were indicatively responsible the fragrant arome revealed by agarwood. The quality of agarwood in the same quality class but originated from different regions revealed the resin content as well as chemical composition which was different fromeach other.

OPTIMASI TEKNIK PEMURNIAN GLUKOMANAN PADA TEPUNG PORANG (Amorphophallus muelleri Blume) Gunawan Pasaribu; Novitri Hastuti; Lisna Efiyanti; Totok K. Waluyo; Gustan Pari
Jurnal Penelitian Hasil Hutan Vol. 37 No. 3 (2019): Jurnal Penelitian Hasil Hutan
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphh.2019.37.3.197-203

Porang (Amorphophallus muelleri Blume) as a non-timber forest product (NTFP) has many advantagescompared to other food crops. In order to the cultivation aspect and excellence of the flour, it made this commodity afood sources in the future. Today, technical post-harvest processing become the current problems especially in gettingoptimum glucomannan. With increasing levels of glucomannan, it made the utilization and marketing of flour will bewider. This study aims to optimize of purification techniques of glucomannan. The research method is through soakingtechniques with ethanol (30%, 40% and 50%) and sodium bisulfite (2%, 3% and 4%). The results showed that theleaching technique ethanol made a significant effect on the increase in glucomannan levels. The immersion technique with50% ethanol and 2% sodium bisulfite can increase glucomannan from 32.65% to 83.96%. The process of soakingwith ethanol not affects the content of ferrum (Fe) and calcium (Ca) in porang flour.

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