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POTENSI HIDROGEL EKSTRAK ETANOLIK DAUN PECUT KUDA (Stachytarpheta Jamaicensis [L.] Vahl) TERHADAP PROSES PENYEMBUHAN ULKUS DIABETIKUM PADA Rattus Novergicus GALUR WISTAR Paulina Maya Octasari; Septiana Laksmi Ramayani
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 8, No 1 (2021)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

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Abstract

Latar belakang:  Terapi utama pada ulkus diabetikum adalah perawatan luka dan pemberian antibakteri. Senyawa fenolik dan flavonoid bersifat antibakteri yang terdapat pada tanaman. Tanaman pecut kuda (Stachytarpheta jamaicensis [L.] Vahl) memiliki senyawa tersebut. Tujuan:  Tujuan penelitian ini adalah untuk mengetahui potensi hidrogel ekstrak daun pecut kuda (EEDPK) terhadap proses penyembuhan ulkus diabetikum pada tikus. Metode:  Pembuatan hidrogel EEDPK  menggunakan bahan HPMC, propilenglikol dan metil paraben dengan  konsentrasi ekstrak sebesar 1%, 3%, dan 5%. Dilakukan penetuan kadar total fenolik dan flavonoid. Tikus diolesi povidon iodine, basis hidrogel, EEDPK 1%, 3%, dan 5% pada ulkus diabetikum pada hari ke-7 hingga ke-21. Proses penyembuhan luka dilihat dengan pemeriksaan leukosit dan makrofag pada hari ke-3, 7, 14, 21. Analisis data menggunakan One Way Anova dilanjutkan dengan Post Hoc (taraf kepercayaan  95%).  Hasil: Hasil penelitian menunjukkan bahwa hidrogel EEDPK mengandung senyawa fenolik dan flavonoid. Semakin tinggi konsentrasi EEDPK memiliki kandungan senyawa yang semakin besar. Semua tikus mengalami penyembuhan luka selama 21 hari. Semua sediaan dapat menurunkan kadar leukosit dan makrofag secara signifikan terhadap kontrol negatif (p<0.05) Simpulan dan saran:  Hidrogel EEDPK dapat menurunkan kadar leukosit dan makrofag pada hari ke-21.
Tablet Hisap Sari Buah Tomat (Licopersicon esculentum Mill.) sebagai Penangkal Radikal Bebas Anasthasia Pujiastuti; Paulina Maya Octasari; Siska Setyorini - Politeknik Katolik Mangunwijaya
Indonesian Journal on Medical Science Vol 6 No 2 (2019): IJMS 2019
Publisher : Unit Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Bhakti Mulial

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Abstract

Abstract : Free radicals are atoms or molecules that are unstable and very reactive because they contain one or more unpaired electrons in their outer orbitals. The body needs an important substance, an antioxidant that is capable of capturing free radicals so that it cannot induce a disease. Antioxidants are contained in various types of food, especially fruits and vegetables. One fruit that contains a lot of antioxidants is tomatoes (Lycopersicum esculentum Mill). Tomatoes have lycopene, flavonoids and vitamin C as antioxidants. Tomato pollen can be made lozenges. The most important material in determining the dissolution time of the lozenges after is the binder, among others, Polyinylpyrrolidone K-30 (PVP K-30). Making tomato lozenges using wet granulation method with the content of PVP K-30 binder used is 1%, 2%, and 3%. The evaluation results of physical characteristics of lozenges were analyzed statistically using ANOVA and Kruskall-Wallis test with a confidence level of 95%, then tested the antioxidant activity of tomato juice lozenges using the DPPH method. Based on the results of the study, it was found that the levels of PVP K-30 had an effect on the physical characteristics of tomato pollen lozenges which included organoleptic, hardness, friability, and dissolution time but had no effect on uniformity of weight and uniformity of size. Antioxidant activity produced by lozenges of tomato juice at a concentration of 300 ppm, formula I of 2.295%; formula II is 2.606%; and formula III 2,391%.Key words: tomato lozanges, tomato juice, PVP K-30, physical characteristics, antioxidant activity  Abstrak: Radikal bebas adalah atom atau molekul yang tidak stabil dan sangat reaktif karena mengandung satu atau lebih elektron tidak berpasangan pada orbital terluarnya. Tubuh memerlukan suatu substansi penting yaitu antioksidan yang mampu menangkap radikal bebas sehingga tidak dapat menginduksi suatu penyakit. Antioksidan terkandung dalam berbagai jenis makanan, terutama buah dan sayuran. Salah satu buah yang banyak mengandung antioksidan adalah tomat (Lycopersicum esculentum Mill). Buah tomat memiliki kandungan likopen, flavonoid dan vitamin C sebagai antioksidan. Serbuk sari buah tomat dapat dibuat sediaan tablet hisap. Bahan yang paling penting dalam penentuan waktu melarut dari tablet hisap setelah adalah bahan pengikat antara lain Polivinilpirolidon K-30 (PVP K-30). Pembuatan tablet hisap sari buah tomat dengan metode granulasi basah dengan kadar bahan pengikat PVP K-30 yang digunakan adalah 1%, 2%, dan 3%. Hasil evaluasi karakteristik fisik tablet hisap dianalisis statistik menggunakan uji anova dan Kruskall-Wallis dengan taraf kepercayaan 95%, selanjutnya diuji aktivitas antioksidan tablet hisap sari buah tomat menggunakan metode DPPH. Berdasarkan hasil penelitian diketahui bahwa kadar PVP K-30 berpengaruh terhadap karakteristik fisik tablet hisap serbuk sari buah tomat yang meliputi organoleptis, kekerasan, kerapuhan dan waktu melarut tetapi tidak berpengaruh pada keseragaman bobot dan keseragaman ukuran. Aktivitas antioksidan yang dihasilkan oleh tablet hisap sari buah tomat pada konsentrasi 300 ppm, formula I sebesar 2,295 % ; formula II sebesar 2,606 %; dan formula III sebesar 2,391 %. Kata kunci: tablet hisap, sari buah tomat, PVP K-30, karakteristik fisik, aktivitas antioksidan
UJI DAYA ANALGETIK DAN ANTIINFLAMASI EKSTRAK ETANOLIK DAUN SINGKONG (Manihot utilissima Pohl.) PADA MENCIT GALUR SWISS Paulina Maya Octasari; Daniar Kusuma Wardani; Elisabeth Lionida Sari
Jurnal Wiyata Penelitian Sains dan Kesehatan Vol 9, No 2 (2022)
Publisher : LP2M IIK (Lembaga Penelitian dan Pengabdian Masyarakat Institut Ilmu Kesehatan) Bhakti Wiy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56710/wiyata.v9i2.615

Abstract

Background: Flavonoids are compounds that can function as analgesics and anti-inflammatory. Cassava leaves are known to contain flavonoids with the main content of rutin. The routine flavonoid content in the ethanolic extract of cassava leaves is 4.987% so that it has the potential to be developed as traditional medicine. Objective: This study aimed to determine the analgesic and anti-inflammatory properties of cassava leaf ethanolic extract (EEDS) and the effect of varying doses of EEDS on analgesic and anti-inflammatory properties. Methods: This study was a completely randomized experimental study with a unidirectional pattern in mice aged 2-3 months and weighing 20-30 grams. Analgesic testing method used the stretching method with 1% acetic acid as the induction, while the anti-inflammatory test used the edema thickness measurement method with 1% carrageenin induction. The results of the percentage of protection, the change in the percentage of protection, and the percentage of anti-inflammatory power were statistically analyzed. Results: The results showed that EEDS was green-black in color, bitter in taste, 7% drying loss, 12.31% w/w yield and positive for flavonoids. EEDS has analgesic power at a dose of 70; 140; and 280 (mg/kgBB) with a percentage of protection of 26.59%; 24.45%; and 21.96%; change in percentage of protection by 65.75%; 68.51%; and 71.72%. EEDS at a dose of 35; 70; and 140 (mg/kgBW) has a percentage of anti-inflammatory power of 44.93%; 37.34%; and 30.75%. Conclusion: The higher the dose of EEDS, the higher the analgesic power but the lower the anti-inflammatory power
Evaluasi Kelengkapan Administratif dan Farmasetis Resep di Poliklinik Anak Rumah Sakit Karanggede Sisma Medika Boyolali Paulina Maya Octasari; Patriana Agustin Windasari
Journal Clinical Pharmacy and Pharmaceutical Science Vol. 1 No. 2 (2022): Oktober: Journal of Clinical Pharmacy and Pharmaceutical Science
Publisher : Prodi D3 Farmasi Politeknik Katolik Mangunwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61740/jcp2s.v1i2.8

Abstract

Prescription screening is one of the pharmaceutical services carried out by reviewing prescriptions according to administrative, pharmaceutical and clinical requirements. Administrative and pharmaceutical requirements ensure the legality of prescriptions, correct identification and successful treatment of patients. The purpose of this study was to evaluate the administrative and pharmaceutical completeness of prescriptions at the Children's Polyclinic Karanggede Sisma Medika Hospital Boyolali. This research was carried out in an observational descriptive manner with retrospective data from prescriptions at the Children's Polyclinic at Karanggede Sisma Medika Boyolali Hospital for the period July – September 2021, with a total sample of 215 prescription sheets. The complete administrative prescription data includes the patient's name, medical record number, gender, date of birth, doctor's name, doctor's initials and date of prescription. Complete prescription pharmaceutical data include the name of the drug, the dosage form, the strength of the preparation, the dosage unit, the amount of the drug, the rules for use and how to use it. The data were analyzed quantitatively to determine the percentage of administrative completeness, pharmaceutical completeness, and both. The study was conducted with 215 prescriptions. The results of this study showed that 43.72% of prescriptions were administratively complete which consisted of 100% of the patient's names. As many as 60% are complete pharmaceutically consisting of the name of the drug, the form of the drug and the amount of the drug by 100%, and 28.37% complete both.
Uji Daya Antiinflamasi Ekstrak Etanolik Daun Mengkudu (Morinda Citrifolia (L.)) Terhadap Mencit Putih (Mus Musculus) Jantan Galur Swiss Yang Diinduksi Karagenin Paulina Maya Octasari; Andreas Napoleon
Journal Clinical Pharmacy and Pharmaceutical Science Vol. 1 No. 2 (2022): Oktober: Journal of Clinical Pharmacy and Pharmaceutical Science
Publisher : Prodi D3 Farmasi Politeknik Katolik Mangunwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61740/jcp2s.v1i2.9

Abstract

Inflammation is the body's response to protect it self from chemicals, infectious agents, and damage to body tissues caused by injury. Flavonoid compounds can be used as anti-inflammatory by inhibiting the activity of cyclooxygenase (COX) and lipoxygenase enzymes. The amount of flavonoid content in noni leaves was 15.97 mg QE/g extract. The difference between this study and the previous research conducted by Rifaldy, et al (2019) lies in the test animals, the method of measuring inflammation, and the dose of extract used. This study aims to determine the anti-inflammatory power and dosage of the ethanolic extract of noni (Morinda citrifolia (L.)) leaves. This study is an experimental study, using a completely randomized design with one-way pattern with one treatment factor, namely variations in the dose of Noni Leaf Ethanolic Extract (EEDM), namely 42 mg/20gBW mice, 56 mg/20gBW mice, and 70 mg/20gBW mice. The anti- inflammatory test method uses artificial edema formation with carrageenin. The research data was in the form of percentage of anti-inflammatory power (%DAI), thenstatistically analyzed using SPSS with 95% confidence level. The results of the anti-inflammatory power of EEDM from the smallest to the largest doses produced anti-inflammatory properties, namely 55.30%, 62.28%, and 69.41%, this means that the larger the dose, the higher the percentage of anti-inflammatory power (p<0.05). The percentage of anti-inflammatory power of EEDM at a dose of 70 mg/20gBW mice had an anti-inflammatory effect that was not significantly different from the positive control (p>0.05).