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Evaluasi Kualitas Spermatozoa Ayam Brahma, Cochin dan Bangkok Sebagai Dasar Penerapan Teknologi Inseminasi Buatan pada Ayam Kampung Asnawi Asnawi; Budi Indarsih; Dewi Haryani; Sukartha Jaya; Maya Nachida; Adji Santoso Dradjat
JURNAL SAINS TEKNOLOGI & LINGKUNGAN 2021: Special Issue, Oktober 2021
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v0i0.250

Abstract

Research on the evaluation of the spermatozoa quality of Brahma, Cochin and Bangkok chickens as the basis for the application of artificial insemination technology in native chickens has been carried out. The purpose of this study was to evaluate the spermatozoa quality of Brahma, Cochin and Bangkok rooster as the basis for determining the spermatozoa dose for artificial insemination in local chickens. method research was used a completely randomized design (CRD) with three treatments, that is: Brahma, Cochin and Bangkok rooster. Each treatment was repeated five times. Cement storage is carried out by inserting an artificial vagina into the cloaca of the rooster and stimulating it to climb the hen. The collected sperm were analyzed at the Animal Reproduction Laboratory, Faculty of Animal Science, Mataram University. The variables observed included motility, viability and morphology of spermatozoa. The results of studied that the spermatozoa motility of Brahma, Chicin and Bangkok rooster were 72 ± 2.74%. 74 ± 2.24% and 73 ± 2.74% (P> 0.05) respectively; The spermatozoan viability of Brahma rooster and Bangkok rooster was 99.2 ± 1.30%, 99.4 ± 0.55% and 99.2 ± 1.10% (P>0.05)respectively. The spermatozoan morphology of brahma rooster, chicin rooster and Bangkok rooster was 94.6 ± 2.30%, 94.4 ± 2.70% and 97.4 ± 2.07% (P> 0.05), respectively. Cochin rooster and Bangkok rooster respectively, 62.6 ± 7.92 x 107, 57 ± 5.83 x107 , and 65.2 ± 12.28 x107, respectively. The results of the study concluded that Brahma, Cochin and Bangkok rooster had good sperm quality and could be able to used in artificial insemination of Kampung hens.
SOSIALISASI TATA CARA SELEKSI CALON PEJANTAN SAPI BALI DI DESA BERIRI JARAK KECAMATAN WANASABE KABUPATEN LOMBOK TIMUR Lalu Ahmad Zaenuri; Adji Santoso Dradjat; rodiah rodiah; lukman HY; I Wayan Lanus Sumadiasa
Jurnal Abdi Insani Vol 6 No 2 (2019): Jurnal Abdi Insani Universitas Mataram
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v6i2.242

Abstract

Ada beberapa alasan ternak sapi harus ditingkatkan terus populasi dan produktifitasnya. Pertama, ternak sapi merupakan sumber pendapatan yang memberikan kontribusi cukup signifikan dalam struktur pendapatan petani peternak. kedua, sapi Bali adalah ternak yang sudah beradaptasi dengan sangat baik selama ratusan tahun di Nusa Tenggara Barat. Ketiga, pemasarannya mudah terbukti permintaan daging sapi selalu lebih tinggi dari ketersediannya. Terakhir, kualitas genetik sapi Bali ditengarai cendrung menurun dari waktu ke waktu akibat seleksi negatip. oleh karena itu, untuk meningkatkan produktifitas dan peran sapi dalam menyediakan daging dan pendapatan peternak, kualitas genetik sapi Bali harus selalu ditingkatkan. Salah satu caranya adalah melalui seleksi pejantan, sehingga kualitas genetic dan produktifitas anak sapi yang lahir dari bibit sapi jantan terseleksi akan meningkat dan pada akhirnya pendapatan peternak juga akan meningkat. Berdasarkan alasan seperti diuraikan diatas, pengabdian kepada masyarakat dengan topik “Sosialisasi Tata Cara Seleksi Calon Pejantan Sapi Bali di desa Beririjarak kecamatan Wanasabe kabupaten Lombok Timur” telah dilaksanakan dengan tujuan untuk meningkatkan pengetahuan dan keterampilan peternak sapi di desa Beririjarak mengenai tata cara seleksi pejantan sapi Bali yang ungul. Hasil pengabdian kepada masyarakat ini menunjukkan bahwa, 100% peserta menyatakan bahwa materi penyuluhan sanngat bermanfaat dan akan diterapkan ketika mereka menseleksi sapi Bali jantan sebagai pejantan. Disarankan supaya materi penyuluhan ini juga perlu disosialisasikan kepada peternak atau kelompok sapi di kecamatan lain atau di Nusa Tenggara Barat pada umumnya.
BOER BUCK SPERM QUALITY IN EXTENDER SUPPLEMETED WITH NON-ENZYMATIC ANTIOXIDANTS AND STORAGE AT COLD TEMPERATURE Lalu Zaenuri; R Rodiah; Adji Santoso Dradjat; Lukman HY; Eny Yuliani
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26913

Abstract

The aim of this study was to determine the durability of standard extender tris egg yolk supplemented with non-enzymatic antioxidants from guava and fig fruit filtrate on the motility and viability of Boer buck sperm. Semen was collected using an artificial vagina every four days for ten replications. The treatment extenders were Tris+2.5% egg yolk (P0) and P0+1.487 mg vitamin A and P0+3.958 mg vitamin C per ml/100 ml (v/v) hereinafter referred as P1 and P2, respectively. The experimental design was a completely randomized 3 times 10 a factorial pattern. The variables assessed were individual motility, progessive motility and viability  of sperm evaluated every day for 4 consecutive days. Result indicated that, 4 days after extended and stored at 5°C, the average individual motility, progessive motility and sperm viability were 45.2±7.94; 33.0±10.2 and 49.6±7.69 for P1, and 45.4±6.14; 30.4±8.38 and 50.5±5.88 for P2. The data on the three indicators of sperm quality between P1 and P2 did not show a significant difference (P0.05). While for P0 30.6±5.85; 24.6±7.30 and 40.7±5.77, respectively, and were significantly lower (P 0.05) than P0 or P1. It was concluded that semen extended using tris-egg yolk extender supplemented with vitamin A or C could still be used for Artificial Insemination until day 4 after extended and storage at 5°C. It is suggested that research needs to be done to produce a ready to use, simple preaparation, cheap and long lasting instant extender.
Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens Asnawi; Maskur; Adji Santoso Dradjat; Muhammad Muhsinin
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5779

Abstract

The success of artificial insemination in chickens is very dependent on sperm quality. Sperm temperature and diluent during storage can affect sperm quality. This study evaluated the quality of Bangkok chicken sperm diluted with 5% glucose and NaCl stored at 5 ⁰C and 26 ⁰C. The research design used was completely randomized. The data obtained were analyzed using a variance. The results showed that the motility of sperm preserved for 9 hours at 26 ⁰C with infused NaCl and Glucose 5% differed significantly (P< 0.05) with motility of 50±0.0% and 34±8.94%, respectively. While preservation under 5 ⁰C for 9 hours with NaCl, better than Glucose 5% (P<0.05) with motility of 58.00±10.95% and 38.00±10.95%, respectively. The viability of sperm preserved at 26 ⁰C with glucose 5% extender was better than NaCl (P<0.05) with a value of 58.93±1.27% and 33.43±1.27%, respectively. While preservation at 5 ⁰C of viabilities of sperm under Glucose 10% and NaCl were not significantly different (P>0.05) from values of 52.57±5.15% and 48.14±8.09%, respectively. The abnormality of sperm stored at 26 ⁰C and 5 ⁰C for 9 hours with NaCl and Glucose 5% were not different (P>0.05). Insemination using 100 million sperm with infused NaCl extender from 18 eggs produced 94.44% (n=17) fertility and 72.22% (n=13) hatchability. While insemination using infuse glucose 5% extender, 11 eggs produced 63.64% (n=7) fertility and 54.54% (n=6) hatchability. The infusing NaCl extender produced better sperm quality, fertility, and hatchability than glucose 5% at 5 ⁰C.