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The Effect of Vegetation Community and Environment on Gyrinops versteegii Growth Rawana Rawana; Suryo Hardiwinoto; Budiadi Budiadi; Sri Rahayu
Jurnal Manajemen Hutan Tropika Vol. 24 No. 1 (2018)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2275.55 KB)

Abstract

Although Gyrinops versteegii has been domesticated by local community in Sragen Regency, the environmental factors and species which have high compatibility with G. versteegii still have not known yet. The objectives of this research were 1) to know which one of those vegetation communities that gives the best the diameter and height growth of the domesticated G. versteegii, and 2) to know the environmental factors that influenced the growth of the diameter and height of the domesticated G. versteegii. Sampling was done systematically with a plot of size 20 × 20m for trees, 10 × 10m for poles, and 5 × 5m for saplings with a total of 28 plots. SPSS Program version 22 was used for cluster, one-way anova, correlation, and multiple regression analysis. The results showed that for achieving the best G. versteegii growth, the G. versteegii should be planted under the community group which was dominated by Paraserianthes falcataria, Tectona grandis, Gliricidia sepium, and Eugenia aquea. Environmental factors affecting diameter growth of G. verteegii were organic carbon of the soil and the vegetation density. Meanwhile the environmental factors affecting the height growth of G. versteegii were temperature, light intensity, relative light intensity, organic carbon, C N ratio, P, Mg, air humidity, and clay content.
KARAKTER MORFOLOGI ISOLAT Phlebiopsis sp.1 JAMUR PENGENDALI HAYATI YANG POTENSIAL UNTUK Ganoderma philippii Desy Puspitasari; Arif Wibowo; Sri Rahayu; Istiana Prihatini; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.664 KB) | DOI: 10.20886/jpth.2016.10.1.51-61

Abstract

Identification of wood decay fungi based on morphological character of isolates is very helpful where identification of fruit body morphology is ambiguous. Nine isolates of Phlebiopsis sp.1 obtained from the isolation root of Eucalyptus pellita in permanent plots with root rot disease have the potential as biological control for G. philippii. The verification based on ITS sequences of rDNA showed that these isolates were closely related to P. gigantea and P. flavidoalba. The characterisation of morphological was performed on isolates grown on Malt Extract Agar media with sawdust. Based on the macroscopic observation, the isolates were categorized into three different morphotypes: (1). Cottony mycelium from the beginning to the advanced stage, one isolate (Pb5); (2). Cottony at the beginning and then turned into farinaceous to floccose on the advanced stage, 7 isolates (Pb1, Pb2, Pb4, PB6, PB8, Pb9, Pb10); (3). Absent at the beginning and then turned into zonate on the advanced stage, one isolate (Pb11). Morphotype 2 is the dominant group of Phlebiopsis sp.1 isolates, with fine tufts of white mycelium which turned brown on the surface of the colonies. Morphotype 3 has very different morphological characters than other 8 isolates, it has very characteristic concentric circle with different color and texture of each circle. The growth rate of mycelium ranged between 10.70 - 10.85 cm²/day for morphotype 1 and 2; 1.95 cm²/day for morphotype 3.
SENYAWA FENOL PADA TOLERANSI Falcataria moluccana (Miq.) TERHADAP PENYAKIT KARAT TUMOR Asri Insiana Putri; Mohammad Na'iem; Sapto Indrioko; Sri Rahayu
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 3 (2015): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.302 KB) | DOI: 10.20886/jpth.2015.9.3.189-202

Abstract

Falcataria moluccana (Miq.) is severely attacked by Uromycladium tepperianum (Sacc.) McAlpine, which is a gall-forming (neoplasmic) and parasitic obligate pathogen. Phenolic compounds have the ability to function as co-factors of pathogenicity determinant of pathogens development and chemical defenses of plants. The purpose of this study was to determine the content of phenolic compounds on gall rust tolerance sengon by observation of tolerant sengon height, quantitative analysis of total phenolic compounds, wood anatomy analysis and tolerance test of sengon callus from tissue culture with filtrate gall rust as chemical agent. The results of the research were (1) inoculated sengon have lower total phenolic compounds than the control (no inoculation), (2) tolerant sengon have lower content of phenolic compounds than sensitive one, (3) microscopic wood anatomy observation shows that tolerant sengon have darker substrat, and (4) in the same concentration of gall rust filtrate incubation media, the tolerant sengon have higher survival cell calli. The highest concentration that callus cells can survive was 25% (v/v).
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection Asri Insiana Putri; Mohammad Na'iem; Sapto Indrioko; Sri Rahayu; Ari Indrianto
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.785 KB) | DOI: 10.22146/ijbiotech.9301

Abstract

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds fromtwo different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets invitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The meanof fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of freshweight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins andquantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attackedand un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. Itis notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminantsof pathogenecity. While the highest total phenolic compound were contained in gall rust extract(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use asmedia selection in vitro.
Karakter Jamur Ceratocystis sp. Penyebab Penyakit Busuk Batang pada Acacia decurrens dan Status Penyakitnya di Taman Nasional Gunung Merapi, Yogyakarta Sri Rahayu; Handojo Hadi Nurjanto; Rahman Gilang Pratama
Jurnal Ilmu Kehutanan Vol 9, No 2 (2015)
Publisher : Fakultas Kehutanan Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.729 KB) | DOI: 10.22146/jik.10193

Abstract

Acacia decurrens merupakan salah satu jenis tanaman yang tumbuh mendominasi kawasan Taman Nasional Gunung Merapi (TNGM), pasca erupsi Gunung Merapi tahun 2010. Sekitar 80% tegakan A. decurrens di kawasan tersebut menunjukkan gejala busuk batang akibat infeksi jamur Ceratocystis sp. yang umumnya dipicu oleh luka gerekan kumbang dari kelompok ambrosia. Penelitian bertujuan untuk : (1) mendeskripsikan karakter morfologi jamur Ceratocystis sp., serta kemampuannya beradaptasi pada beberapa jenis tanaman hutan, (2) mengevaluasi status penyakit busuk batang oleh jamur Ceratocystis sp. Karakter morfologi dan kemampuan adaptasinya pada inang akasia, melina, jabon, sengon, dan jati dilakukan di Laboratorium Perlindungan dan Kesehatan Hutan, Fakultas Kehutanan UGM. Survei untuk evaluasi status penyakit busuk batang dilakukan pada bulan Februari sampai Agustus 2014 di demplot restorasi pasca erupsi Merapi (luas 8,4 ha), dengan intensitas sampling 8%. Berdasarkan karakter morfologi, terdapat 2 isolat jamur Ceratocystis sp. yaitu asal lembah (L) dan dari bukit (B) dengan warna koloni krem, luas koloni 20-22 cm2 pada umur 14 hari, membentuk konidia menyerupai tong, dan silindris. Sifat lainnya yaitu memiliki kemampuan yang sama untuk tumbuh, mengkolonisasi, dan menginfeksi inang akasia, sengon, jabon, dan melina, tetapi tidak mampu tumbuh pada inang jati. Berdasarkan luas serangan, status penyakit busuk batang berkisar antara sangat umum sampai menyebar luas (luas serangan = 54-100%), dengan tingkat keparahan bekisar antara ringan sampai parah (intensitas penyakit = 15-67%).Kata kunci: Ceratocystis sp., Acacia decurrens, luas serangan, intensitas penyakit, Taman Nasional Gunung Merapi. Characteristic of stem rot diseases caused by Ceratocystis sp. on Acacia decurrens and its status in Gunung Merapi National Park, YogyakartaAbstractMount Merapi National Park (TNGM) has been dominated by Acacia decurrens after the eruption in 2010. Almost 80% of A. decurrens trees showed stem rot diseases caused by Ceratocystis sp. which may associate with stem wound induced by ambrosia beetle and other physical injuries. The research objective were (1) to characterize the morphological feature, in vitro growth, and ability to adapt, colonize as well as to infect akasia, jabon, sengon, melina and jati sedlings, (2) to evaluate the status of stem rot disease in TNGM demonstration plot. Laboratory work was conducted in order to study the morphological feature of the fungus, in vitro growth on PDA media, and to evaluate its compatibility to growth, colonize, and infect on 5th month seedling of akasia, sengon, jati, jabon and melina. Field monitorings were conducted from February to August 2014 at the restoration plot (8.4 ha) at 8% sampling intensity. Disease status was evaluated in order to ascertain the disease incidence and severity of stem rot disease at the demonstration plot area. Two Ceratocistys isolates found from the hill (B) and valley (L) had similar characteristics on morphologic features i.e. cream color, 20 to 22 cm2 colony size at 14 days growth in PDA media, having both cylindrical and barrel shaped conidia. The other characteristics of the Ceratocistys were an ability to growth, to colonize, and to infect akasia, sengon, melina and jabon, except on jati. The status of stem rot disease was ranged from highly common to widespread (disease incidence = 54%-100%) as the disease severity status was ranged from low to severe (disease severity = 15%-67%).