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All Journal Berkala Ilmu Kesehatan Kulit dan Kelamin Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Prominentia Medical Journal
Priangga Adi Wiratama
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Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Nephroprotective effect of virgin coconut oil in Plasmodium berghei ANKA infected Balb/c mice Syafarinah Nur Hidayah Akil; Heny Arwati; Puspa Wardhani; Priangga Adi Wiratama
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 5, No 2 (2021)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v5i2.5791

Abstract

AbstractMalaria is a parasitic infectious disease caused by Plasmodium, which remains a world health problem with an estimated 219 million cases worldwide. In severe malaria infection, several organs of the body can be affected, including the kidneys. One of the pathophysiology associated with the worsening of this disease is oxidative stress. The use of antioxidants is expected to prevent this, and one product that has a high antioxidant content is virgin coconut oil (VCO). This study aimed to analyze the effect of VCO on the kidney in Plasmodium berghei ANKA-infected mice. This study was an in vivo laboratory experimental study with a randomized post-test only control group design using 35 BALB/c mice infected with P. berghei ANKA, weighing 20-30 grams. VCO with the Javara® brand is used with doses of 1, 5, and 10 ml/kg body weight (kgBW)/ day. The parameter assessed were levels of BUN, creatinine, and renal histopathological changes. The administration of VCO on the treated group shows minimal tubular necrosis and glomerulonephritis compared to the negative control group. The BUN and creatinine levels in the treated group were also lower than the negative control group. The results showed that VCO has a nephroprotective effect against P. berghei ANKA infection in mice.Keywords: malaria, kidney, virgin coconut oil
Perubahan Jumlah Sel Goblet Pada Cavum Nasi Tikus Wistar Jantan Akibat Rokok Elektrik Azka Nadya Mifta; Hudi Winarso; Etha Rambung; Priangga Adi Wiratama
Bahasa Indonesia Vol 2 No 1 (2021): Prominentia Medical Journal
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/pmj.v2i1.2262

Abstract

Nikotin, karbonil, TSNA (Tobacco-Spesific Nitrosamine), dan metal adalah beberapa zat yang terkandung dalam asap rokok elektrik. Zat-zat ini dapat menyebabkan hiperplasia, metaplasia, apoptosis sampai nekrosis sel. Penelitian eksperimental post-test only control design ini mengamati perubahan jumlah sel goblet pada mukosa respiratori cavum nasi tikus wistar jantan setelah pemaparan asap rokok elektrik dengan dosis bervariasi. Penelitian menggunakan 40 ekor tikus wistar jantan berusia 2-2,5 bulan dan berat antara 250-300 gram yang terbagi dalam empat kelompok yakni kelompok K- (kontrol negatif) tidak dipapari asap dan tidak dimasukkan ke dalam smoking chamber, kelompok K+ ( kontrol positif) tidak dipapari asap tetapi dimasukkan ke smoking chamber, kelompok P1 diberikan paparan 15 kali/hari dan kelompok P2 diberikan paparan 30 kali/hari. Paparan asap diberikan selama 54 hari. Tikus di euthanasia hari ke-55, dan diambil sampel cavum nasi kemudian diproses menjadi sediaan histopatologi. Analisis menggunakan uji One Way ANOVA untuk menghitung perubahan jumlah sel goblet. Hasil yang diperoleh selanjutnya dianalisa dengan uji Tukey HSD. Hasil yang diperoleh menunjukkan perbedaan jumlah sel goblet yang bermakna (p0.000 dan 0.004) antara kelompok tikus yang dipapari dengan kelompok yang tidak dipapari asap rokok. Kesimpulan penelitian adalah terjadi penurunan jumlah sel goblet akibat paparan asap rokok elektrik.
Evaluation of Histopathology Findings of Clinically Confirmed Psoriasis Vulgaris Sawitri; Fadila, Arisia; M. Yulianto Listiawan; Priangga Adi Wiratama; Dwi Murtiastutik; Evy Ervianti; Linda Astari; Damayanti; Diah Mira Indramaya; Afif Nurul Hidayati; Medhi Denisa Alinda
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 35 No. 1 (2023): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V35.1.2023.21-26

Abstract

Background: Psoriasis vulgaris can be diagnosed clinically. A biopsy is usually used to confirm non-classic cases. The findings of the histopathology feature are not usually present, thus clinical relevance is needed to confirm the diagnosis. Purpose: To describe the histopathology findings from tissue samples of histopathologically confirmed psoriasis vulgaris reported in the Department of Pathology and Anatomy of Dr. Soetomo General Hospital. Methods: A descriptive-retrospective study of pathology-ascertained tissue samples of histopathologically confirmed psoriasis vulgaris was reported in the Department of Pathology and Anatomy from patients who were also previously diagnosed clinically with psoriasis vulgaris in the Dermatology and Venereology Outpatient Unit of Dr. Soetomo General Academic Hospital over a period of 2 years. Morphological parameters were observed after histopathology sections were stained with hematoxylin and eosin. Result: Thirty-three tissue samples were examined. Parakeratosis (86%) and hypogranulosis (70.3%) were the most observed findings. Club-shaped rete ridges, suprapapillary plate thinning, and spongiform pustules of Kogoj were the least observed findings (each by 2.7%). Three tissue samples (8.1%) showed only parakeratosis. Only 1 tissue sample (2.7%) showed 4 features (absence of the granular layer, parakeratosis, microabscess of Munro, and spongiform pustules of Kogoj). No tissue sample showed every histopathology finding of psoriasis vulgaris. Conclusion: All of the histopathology features of psoriasis vulgaris are rarely found in one tissue section. Albeit histopathology is believed to be the gold standard diagnosis of psoriasis vulgaris, relevance with clinical findings is still mandatory to support the diagnosis.
Co-Authors Afif Nurul Hidayati Azka Nadya Mifta Damayanti Diah Mira Indramaya Dwi Murtiastutik Etha Rambung Evy Ervianti Fadila, Arisia Heny Arwati Hudi Winarso Linda Astari, Linda M. Yulianto Listiawan Medhi Denisa Alinda, Medhi Denisa Puspa Wardhani Puspa Wardhani Sawitri Syafarinah Nur Hidayah Akil