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All Journal Nexus Kedokteran Komunitas Nexus Biomedika Nexus Kedokteran Translasional Nutriology: Jurnal Pangan, Gizi, Kesehatan Proceeding of International Conference Health, Science And Technology (ICOHETECH)
Ratih Puspita Febrinasari
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Fatimah R, Siti; ., Kisrini; Puspita Febrinasari, Ratih
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background:Stevia’s leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of stevia’s ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (stevia’s ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilk’s test of normality and Friedmann test (α = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (α = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistar’s blood glucose level which was given stevia’s ethanol extract or honey. Stevia’s ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistar’s blood glucose on diabetic model Conclusions:Stevia’s ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.
Hepatoprotective Effect of Ethanolic Extract of Pegagan Leaves (Centella asiatica L. Urban) on Histological Damage of Hepatocyte on Mice Model induced by Parasetamol Naila Shofwati Putri; Suyatmi .; Ratih Puspita Febrinasari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Pegagan leaves (Centella asiatica L. Urban) contain antioxidative compounds that can protect hepatocytes from free radicals. The aim of this research was to know the hepatoprotective effect of ethanolic extract of pegagan leaves (Centella asiatica L. Urban) on histological damage of hepatocyte on mice model induced by paracetamol. Methods: This study was an experimental laboratory with the post test only control group design. Samples were 30 male Swiss webster mice, with 2-3 months old and 20 gr body weight divided into 5 groups equally. KN and KI were given distilled water for 14 days. In addition, KI was also given paracetamol in dose 5.07 mg/20 gbw on the 12th, 13th, 14th day. KP1, KP2, and KP3 were given ethanolic extract of pegagan leaves in dose 5 mg, 10 mg, and 20 mg per 20 gbw respectively for 14 days and paracetamol in dose 5.07 mg per 20 gbw on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for preparation with paraffin block method and stained with HE. The features of histological damage of hepatocyte were assessed by counting the numbers of nucleus damage of hepotocyte (pyknosis, karyorrhexis, karyolysis) of 100 cells in the sentrolobular zone. The data were analyzed by One-way ANOVA test and Post Hoc LSD test. \ Results: The means of histological damage of hepatocyte were 16.08 5.160; 66.00 3.275; 44.58 5.583; 31.67 2.188; 26.17 3.810 for KN, KI, KP1, KP2, KP3 respectively. The result of One-Way ANOVA test was *p = 0.000. The result of Post Hoc LSD test showed a significant difference between five groups, *p = 0.000 for KN-KI, KN-KP1, KN-KP2, KN-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3 and *p = 0.002 for KP2-KP3. Conclusion: The ethanolic extract of pegagan leaves can prevent histological damage of hepatocyte on mice model induced by paracetamol. Keywords: ethanolic extract of pegagan leaves, paracetamol, histological damage of hepatocyte
Efek Nefroprotektif Ekstrak Daun Sirsak (Annona muricata L.) pada Mencit Model yang Diinduksi Parasetamol Nur Hidayah; . Suyatmi; Ratih Puspita Febrinasari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Soursop Leaf (Annona muricata L.) contains active compounds that have antioxidant effects consisting of flavonoid, alkaloid, tannin, triterpenoid and steroid. The aim of this research is to know the protective effect of Soursop Leaf Extract on the renal histological damage of mice induced by paracetamol Methods: This study was experimental research with the post test only controlled group design. Samples were 30 male Swiss webster mice, 2-3 months old and 20 g of body weight. Samples were divided randomly into 5 groups, each group consisted of 6 mice. The normal group (KN) and negative control group (KKn) were given distilled water for 14 days. KP1, KP2, and KP3 were given soursop leaf extract in dose 5.6 mg, 11.2 mg, and 22.4 mg per g of body weight of mice, respectively, for 14 days. Paracetamol in dose 5.07 g per 20 g body weight of mice were given to KKn, KP1, KP2, and KP3 on the 12th ,13th ,14th days. On the 15th day mice were sacrificed and then renal organ were taken for histological preparation and stained with HE. Renal histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of 50 epithelial cells from proximal tubule of right and left renal organ. Data were analyzed using One-Way ANOVA test and continued with Post Hoc (LSD) test. Results: The means of necrotic cells for KN, KKn, KP1, KP2, and KP3 were 8.17, 38.58, 26.92, 21.08 and 11.67, respectively. The One-Way ANOVA test showed a significant difference of renal damage score in five groups with p = 0.000. The Post Hoc LSD test also showed significant difference among five groups, p = 0.000. Conclusion: Soursop leaf extract can prevent the histological damage of renal cells on mice induced by paracetamol. Keywords: Soursop leaf extract, paracetamol, renal histological damage
Efek Hepatoprotektif Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Riris Arizka Wahyu Kumala; . Suyatmi; Ratih Puspita Febrinasari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Soursop leaves (Annona muricata L.) contains alkaloid, flavonoid, saponin, triterpenoid, and steroid that are potent antioxidant to protect the liver from free radicals. The aim of this research was to know the hepatoprotective effect of soursop leaves extract toward liver histological damage of mice (Mus musculus) induced by paracetamol. Methods: This was an experimental laboratory research with the post test only controlled group design. Samples were 30 male Swiss Webster mice with 2-3 months old and 20g of weight taken by incidental sampling. They were divided randomly into 5 groups, namely kelompok kontrol negatif (KKn), kelompok kontrol (KK), kelompok perlakuan 1 (KP1), kelompok perlakuan 2 (KP2), and kelompok perlakuan 3 (KP3). KKn and KK were given aquadest for 14 days. Soursop leaves extract were given to KP1, KP2, KP3 in dose 5.6 mg, 11.2 mg, and 22.4 mg/20gbw of mice respectively for 14 days. Paracetamol in dose 5.07 g/20gbw were given to KK, KP1, KP2, and KP3 on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for histological preparation with paraffin block method and HE staining. Liver histological features were assessed based on quantifying of pyknosis, karhyorhexis, and karyolysis of each 100 cells on the 3rd zone. Data were analyzed using the One-Way ANOVA test (? = 0,05) and continued with Post Hoc (LSD) test (? = 0,05). Results: The results of One-Way ANOVA test showed a significant difference of liver damage score among groups, *p = 0.000. Results of LSD test showed significant difference between KKn-KK, KKn-KP1, KKn-KP2, KKn-KP3, KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, KP2-KP3 with *p = 0.000 (p < 0.05). Conclusion: Soursop leaves extract (Annona muricata L.) can prevent liver histological damage of mice (Mus musculus) induced by paracetamol.Keywords: Soursop leaves extract, paracetamol, histological damage of liver cell
Screening The Effectiveness of Menirans Leaf Extract (Phyllanthus niruri L.), Pepaya (Carica papaya L.), and Kunyit (Curcuma longa L.) as Antidengue In Vitro Hutami Sri Ummiyati; Ratih Puspita Febrinasari; Leli Saptawati
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Introduction: Dengue Haemorrhagic Fever (DHF) is an infectious disease that is caused by dengue virus. There were 71.668 DHF cases with 641 mortalities in Indonesia which was recorded from early January to mid-December 2014. Recently, DHF doesnt have a specific therapy. The aim of this study was screening about natural extracts in Indonesia as antidengue. Methods: This study was a laboratory experimental research with dengue virus serotype 2 strain New guinea C (DENV2 NGC) as a subject. This virus was infected into Huh-7 cell line. The study was conducted in 2 parts. First, dengue virus solution was mixed with natural extract and then this solution was infected to Huh-7 cell monolayer to obtain percent infectivity by FFU assay. Second,the natural extract was exposed to Huh-7 cell monolayer to obtain percent viability by MTT assay. The effective extract was selected if the extract had average percent infectivity 20% and average percent viability > 50%. Results: The percentage of viability of menirans leaf extract (Phyllanthus niruri L.), papaya (Carica papaya L.), and turmeric (Curcuma longa L.) were 96.2%, 97.5%, and 88.7% respectively. While their average percentage of infectivity were 63.9%, 10.5%, and 53.6%. Conclusion: The papayas leaf extract (Carica papaya L.) was effective as antidengue in vitro. Keywords: DENV2 NGC,dengue, meniran, papayas leaf, turmeric
Effect of EthanolicExtract of Centella asiatica againts Adrenal Gland CortexCell Lipid Droplet Feature Induced by Chronic Immobilization Stress in Rats Mochammad Fairuz Zahran; Brian Wasita; Nanang Wiyono; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Chronic stress can increase the lipid droplet in cortex cell and adrenal gland damage. Pegagan extract contains such as asiaticoside, flavonoids and triterpenoid are known have antioxidant effect. The objective of the present study was to investigate the beneficial effects of Ethanolic Extract of Centella asiatica (EEC) on adrenal gland cortexcell lipid droplet feature induced by immobilization stress in male rats. Method: This study was a laboratory experimental study with randomized post test only control group design. Paraffin blocks from thirty five male rats previously subjected with chronic immobilization stress and then divided into 6 groups: control group given distilled water 1 mg/kg, stress + PGA, stress + 150 mg/kg EEC, stress + 300 mg/kg EEC, stress + 600 mg/kg EEC and stress + fluoxetine 10 mg. The treatments were performed for 21 consecutive days. Lipid droplet was evaluated using Scoring Lipid Content from Hematoxylin Eosin staining. Statistic analysis was performed using Kruskall-Wallis test continued by Post Hoc Mann-Whitney Test with Bonferroni correction. Result: Results of Kruskall Wallis test showed significant differences in six groups with p = 0.000. Mann Whitney test with Bonferroni correction showed a significant difference between control group with stress + PGA and stress + 600 mg/kg EEC. Fluexetine as positive control show the most effective dose in this study (p = 0,000). There were no significant difference between fluoxetine as control positive and control group (p = 0,112). Conclusion: Stress + 600 mg/kg EEC has beneficial effect on preventing adrenal gland cortexcell lipid droplet feature induced by chronic immobilization stress in rat. Keywords : Centella asiatica, chronic immobilization stress, adrenal glands lipid droplet feature
Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Siti Fatimah R; Kisrini .; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background:Stevias leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of stevias ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (stevias ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilks test of normality and Friedmann test (? = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (? = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistars blood glucose level which was given stevias ethanol extract or honey. Stevias ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistars blood glucose on diabetic model Conclusions:Stevias ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.
The Effect of Soybean Powder (Glycine max) to the Histological Structure of Mices (Mus musculus) Liver Cells Induced by Used Cooking Oil Selvia Anggraeni; Endang Listyaningsih Suparyanti; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Soybean contain antioxidants which have hepatoprotective effect; one of them is isoflavone. Soybean powder has the highest isoflavone among soybean products. This research aims to prove that the use of soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Methods: This study was an experimental laboratory with a post-test only control group design. Twenty eight Swiss Webster male mice were divided into 4 groups. Negative control group (KN) was given distilled water, the positive control (KP) was given 0.06 ml/ 20 g BW used cooking oil, dose 1 (KD1) was given soybean powder 5.6 mg/ 20 g BW and used cooking oil, and dose 2 (KD2) was given soybean powder 11.2 mg/ 20 g BW and used cooking oil. Soybean powder was given for 14 days, while the used cooking oil was given orally on 8th-14th day. On the 15th day, mice were sacrificed, taken their liver, and made ??preparations with HE staining. Liver cell damage was observed by counting the number of cells with pyknosis, karyorheksis, and karyolysis of 100 cells in liver centrolobular zone. Data were analyzed by statistical tests One-way ANOVA (Analysis of Variant) (? = 0.05). Results: The highest number of liver cells damage is on KP, followed by KD1, KD2, and at least on KN. Results of One-way ANOVA test showed significant differences among the four groups with p = 0.000 (p < 0.05). Results of Post Hoc Test of Multiple Comparasions using LSD test also showed significant differences between KN-KP, KN-KD1, KD2-KN, KP-KD1, KD2-KP, KD1-KD2 with respectively p = 0.000 (p < 0, 05). Conclusion: Soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Keywords: soybean powder, used cooking oil, liver histological structure
Hubungan Lama Penggunaan Kontrasepsi Oral Kombinasi dengan Tekanan Darah pada Wanita Usia Subur Mustiqa Febriniata; Widardo .; Ratih Puspita Febrinasari
Nexus Kedokteran Komunitas Vol 1, No 2 (2012): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Combined oral contraception is one of contraception methods that contains estrogen and progesterone. Both of that hormones have an effect on blood pressure levels because of the using duration of combined oral contraception, Body Mass Index (BMI), and acceptor age. The purpose of this research is to analyze the correlation between the using duration of combined oral contraception and blood pressure levels among fertile women in Jaten area, Karanganyar. Methods: This research used observational cross sectional analytic approach method. The population were fertile women who use combined oral contraception. The total sample were 48 patients who appropriate with inclusion and exclusion criterias. The data were collected by kuesioner, interview, anthropometry measurement, and blood pressure direct measurement. Those variables such as the using duration of combined oral contraception, blood pressure levels (systolic and diastolic), age, and body mass index (BMI) were analyzed using Kolmogorof-Smirnovs test of normality and linear regression analysis using SPSS 16 for Windows. Results: The result of simple statistic test using linear regression showed the correlation significancy between the using duration of combined oral contraception and blood pressure levels, both systolic (p=0,004) and diastolic (p=0,012). Conclusions: There is a correlation between the using duration of combined oral contraception and blood pressure levels among fertile women. Keywords: The using duration of combined oral contraception, Blood Pressure, Age, Body mass index
Uji Sitotoksik Ekstrak Daun Andrographis paniculata Ness. pada Kultur Limfosit T Pasien Systemic Lupus Erythematosus Dedi Febriandaru; Ratih Puspita Febrinasari; Lilik Wijayanti
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Systemic Lupus Erythematosus or SLE is a chronic autoimmune disease with varied clinical manifestations. SLE prevalence in Indonesia is being dominated with female, especially in their productive age. Certain etiology of SLE is still unknown until now, but genetic factors and immune system are being estimated as the primary factors. The first line therapy of SLE is cyclophosphamide. Unfortunately if being consumed more than six months, cyclophosphamide has several side effects, like bone marrow depression, infections, alopecia, hemorrhagic cystitis, and infertility. Because of that side effects, herbal medicine is being developed nowday. One of them is Andrographis paniculata Ness. leaf. The aim of this study was quantitative cytotoxic test for Andrographis paniculata Ness. leaf on T lymphocyte culture of Systemic Lupus Erythematosus. Methods: This study was anexperimental study. Subjects in this study were patients with SLE who appropriated SLEs criterias and normal people without allergy and autoimmune diseasess history. This study was done by doing T lymphocyte culture from intravenouss blood sample and observing cells absorbance with ELISA reader. Collected data were used for determining live cells percentage (IC50) and analyzed with Statistical Product and Service Solution (SPSS) 11.00 for Windows. Mean resistance data was tested by linear regression test. Results:The result showed that IC50 dose of Andrographis paniculata Ness. leaf extract treatment on SLE samples was smaller than the normal people. In Andrographis paniculata Ness. leafs extract treatment, IC50 in SLE = 0.00074 and in normal people = 0.19584. Conclusion: Andrographis paniculata Ness.leaf extract had stronger cytotoxic effect on T lymphocyte culture of patients with SLE than the normal people. Keywords: Systemic lupus erythematosus, Andrographis paniculata Ness. leaf extract, T lymphocytes, IC50
Co-Authors . Suyatmi . Widardo AGYA GHILMAN FAZA Berlian Permata Sakti Brian Wasita Dedi Febriandaru Endang Listyaningsih Suparyanti Hanif Mustikasari Hartono Hartono Hudiyono . Hutami Sri Ummiyati Kisrini . Leli Saptawati Lilik Wijayanti Maryati, Warsi MIFTAKHUL JANNAH Mochammad Fairuz Zahran Mustiqa Febriniata MUTHMAINAH . Naila Shofwati Putri Nanang Wiyono Nur Hidayah Riris Arizka Wahyu Kumala Selvia Anggraeni Setyo Sri Rahardjo Shinta Retno Wulandari Siti Fatimah R Suyatmi . Widardo .