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All Journal International Journal of Public Health Science (IJPHS) Indonesian Journal of Applied Sciences At-Taqaddum THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Biologi Tropis Klinikal Sains (Jurnal Analis Kesehatan) Indonesian Journal of Global Health research Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas Jurnal Kesehatan Kusuma Husada Jurnal Farmasetis GEMBIRA (Pengabdian Kepada Masyarakat) Jurnal Pengabdian dan Pemberdayaan Kesehatan
Muhammad Taufiq Qurrohman
Sekolah Tinggi Ilmu Kesehatan Nasional
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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GAMBARAN KADAR GLUKOSA DARAH TERHADAP PERTUMBUHAN Candida albicans PADA DIABETES MELLITUS LANSIA Zuzun Farida; Muhammad Taufiq Qurrohman
Journal of Nursing and Health Vol. 9 No. 4 (2024): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v9i4.398

Abstract

GAMBARAN JUMLAH KOLONI Candida albicans TERHADAP KADAR GULA DARAH SEWAKTU PADA WANITA PENDERITA DIABETES MELLITUS Anik Puspita Ningrum; Muhammad Taufiq Qurrohman
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 1 (2024): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i1.4632

Abstract

Diabetes mellitus terjadi dikarrenakan adanya gangguan pada metabolisme karbohidrat yang tidak stabil sehingga peningkatan kadar gula darah. Kenaikan kadar gula darah ini menyebabkan pertumbuhan jamur Candida albicans. Penelitian ini bertujuan untuk menilai jumlah koloni Candida albicans dalam urin pasien diabetes mellitus terkait dengan kadar glukosa darah sewaktu (GDS). Metode yang digunakan pada penelitian ini adalah metode deskriptif, yaitu dengan menggunakan metode pemeriksaan kadar glukosa darah sewaktu (GDS) menggunakan point-of-care testing (POCT), dan pemeriksaan kadar glukosa urine menggunakan metode celup. Penelitian dilakukan di laboratorium parasitologi Sekolah Tinggi Ilmu Kesehatan Nasional Surakarta, dengan teknik sampling purposive sampling terhadap 19 responden. Hasil penelitian menunjukkan bahwa nilai GDS berkisar antara 101 mg/dL hingga 313 mg/dL, dengan mayoritas responden memiliki GDS di atas batas normal. Pada wanita penderita diabetes mellitus, mayoritas hasil pemeriksaan glukosuria negatif, namun satu responden menunjukkan hasil positif. Temuan penelitian menunjukkan adanya pertumbuhan jamur Candida albicans pada urine wanita penderita diabetes mellitus di Puskesmas Karanganyar. Dengan demikian, kesimpulan yang didapatkan dari penelitian ini adalah mayoritas respondendengani kadar GDS yang melebihi batas normal terdapat peningkatan jumlah koloni Candida albicans.
Detection Candida albicans In The Urine Of Women With Diabetes Mellitus Using The PCR Method Elfira Dwi Novi Nastiti; Muhammad Taufiq Qurrohman
Klinikal Sains : Jurnal Analis Kesehatan Vol. 13 No. 1 (2025): Juni
Publisher : LPPM Universitas Abdurrab

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Abstract

Candida albicans is a fungus that has dimorphic abilities that can grow in the form of yeast or hyphae. Candida albicans fungus can be found in various parts of the human body, including the urinary tract. In individuals with Diabetes Mellitus, high blood glucose levels can increase the risk of fungal infections, because glucose in the urine provides an environment that supports fungal growth. Candida albicans fungus can be detected molecularly which aims to identify DNA in the fungus and the initial molecular stage carried out is DNA isolation. The purpose of this study was to determine the presence or absence of Candida albicans fungus in urine isolate samples of women with diabetes mellitus at the Pajang Health Center, Surakarta City. The research method used was descriptive using a cross-sectional approach. The samples used were urine samples of women with diabetes mellitus at the Pajang Health Center, Surakarta City. Based on the results of the study, data analysis and discussion in this study, it can be concluded that Candida albicans fungus can be detected in the ITS1 region at 219 bp and ITS2 at 338 bp using the Polymerase Chain Reaction (PCR) method.
PELATIHAN PEMBUATAN PASTA GIGI HERBAL BERBAHAN SIWAK UNTUK MENINGKATKAN LITERASI KESEHATAN GIGI PADA IBU PKK DESA BENTAKAN Muhammad Taufiq Qurrohman; Mastuti Widi Lestari; Erlintang Surya Shalesa; Fadhila Saffa Nur Ramadhani; Francisca Pingky Artha Mevia
Jurnal Gembira: Pengabdian Kepada Masyarakat Vol 4 No 02 (2026): APRIL 2026
Publisher : Media Inovasi Pendidikan dan Publikasi

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Abstract

Kesehatan gigi dan mulut merupakan bagian penting dari kesehatan masyarakat. Salah satu upaya yang dilakukan adalah pemanfaatan bahan alami seperti siwak (Salvadora persica) yang memiliki aktivitas antibakteri terhadap bakteri penyebab plak. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan masyarakat dalam menjaga kesehatan gigi dan mulut. Metode yang digunakan penyuluhan dan demonstrasi produk pasta gigi herbal dengan bahan siwak, baking soda, minyak kelapa, bentonite clay, dan peppermint essential oil. Kegiatan diikuti oleh 20 peserta dan berlangsung secara interaktif. Hasil kegiatan menunjukkan adanya peningkatan pemahaman peserta mengenai pentingnya kesehatan gigi dan mulut serta kemampuan dalam membuat produk pasta gigi herbal secara mandiri. Produk yang dihasilkan memiliki tekstur yang baik, aroma segar, dan berpotensi menjadi alternatif produk perawatan gigi berbahan alami. Kegiatan ini diharapkan dapat meningkatkan kesadaran masyarakat dalam menjaga kesehatan gigi dan mulut melalui pemanfaatan bahan alami.
Hepatotoxicity effect of alcoholic beverages on histology and IL-6 gene detection in Rattus norvegicus using polymerase chain reaction Fitria Diniah Janah Sayekti; Vector Stephen Dewangga; Muhammad Taufiq Qurrohman
International Journal of Public Health Science (IJPHS) Vol 15, No 1: March 2026
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v15i1.27001

Abstract

The liver is an organ that has an important role in metabolic and detoxification processes. Alcohol consumption will cause metabolic disorders in the liver and can cause changes in histological structures. Interleukin-6 has a profound role in liver pathology and plays an important role in the body's defense mechanism, and contributes to the manifestation of tissue damage. This study was an experimental study using 20 Rattus norvegicus treated with 25% alcohol, and a normal treatment only given distilled water. Histological observations were carried out on each preparation in 5 fields of view. The hepatotoxicity effect of alcohol was observed based on the presence of cell degeneration and necrosis in histological preparations stained with hematoxylin eosin. IL-6 detection with polymerase chain reaction was analyzed qualitatively using electrophoresis. Based on the statistical test results, the sig. (2 tailed) of 0.00 < 0.05 so that it can be seen that there is a significant difference in histological results between the normal control group without treatment and the group induced by alcohol. The administration of alcoholic beverages with 25% ethanol content in vivo for 21 days causes necrosis and bleeding around the hepatocyte cells. IL-6 overview appeared to be stronger in the group with alcohol-induced drinking, it could indicate that giving alcohol causes a higher inflammatory response. This study provides scientific evidence that alcohol consumption significantly damages the liver. Further research can be conducted to increase the sample size and quantitatively assess IL-6 expression.
Profile of MYH9 SNP RS3752462 Gene Polymorphism in Chronic Kidney Disease (CKD) Patients with Hypertension Nabiilah Nuur Ainii Heryanti; Muhammad Taufiq Qurrohman
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11711

Abstract

Chronic kidney disease (CKD) and hypertension are closely related conditions and can be influenced by genetic factors, including polymorphisms in the Myosin Heavy Chain 9 (MYH9) gene. The SNP rs3752462 in the MYH9 gene is known to be associated with impaired kidney function. This study aims to describe the genotype pattern of the MYH9 rs3752462 SNP polymorphism in patients with chronic kidney disease and hypertension at UNS Hospital. This is a descriptive study with a molecular analysis approach. A total of 10 blood samples were collected. Polymorphism detection was performed using the Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) method with the RsaI restriction enzyme. The results showed that all samples were successfully amplified, producing a PCR product of approximately 421 bp. The DNA bands observed in all samples did not form a consistent combination and did not match the expected genotype reference standard. In addition, the appearance of additional bands outside the target fragment size, therefore the results of this study do not adequately represent the MYH9 SNP rs3752462 gene polymorphism in the studied samples. Conclusion: Further examination is needed using other modern methods such as DNA sequencing and larger sample sizes to obtain appropriate results.
Overview of MYH9 Gene Polymorphism SNP RS3752462 in Chronic Kidney Disease (CKD) Patients with Diabetic Nephropathy Ahmad Akmal Maulana; Muhammad Taufiq Qurrohman
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11712

Abstract

Diabetic nephropathy is a major cause of chronic kidney disease and is influenced by both environmental and genetic factors. One of the genes implicated in the pathogenesis of diabetic nephropathy is MYH9, particularly the Single Nucleotide Polymorphism (SNP) rs3752462. This study aimed to describe the polymorphism of the MYH9 gene SNP rs3752462 in patients with chronic kidney disease associated with diabetic nephropathy. A descriptive study design was applied, involving five patients who met the inclusion criteria. Blood samples were analyzed using the Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) method with the RsaI restriction enzyme. The Results showed that the DNA band patterns observed in all samples did not form consistent combinations and did not correspond to the expected genotype classification standards. In addition, extra bands outside the target fragment sizes and variations in band intensity among samples were observed. Based on these findings, the resulting band patterns could not be reliably used to accurately determine genotypes, and therefore did not adequately represent the polymorphism of the MYH9 gene SNP rs3752462 in the studied samples. Accordingly, further optimization of the PCR-RFLP method is required, along with validation using more accurate approaches, such as DNA sequencing, and a larger sample size to obtain more reliable results.
Pemanfaatan Minyak Jelantah dalam Upaya Menekan Resistensi Bakteri Ditilik dari Kajian Pemeriksaan Molekuler di Desa Ngombakan Polokarto Kabupaten Sukoharjo Vector Stephen Dewangga; Sulasmi Sulasmi; Muhammad Taufiq Qurrohman; Rachma Aulia Putri; Restu Ardhiyanto
Jurnal Pengabdian dan Pemberdayaan Kesehatan Vol. 3 No. 3 (2026): September 2026
Publisher : Menara Science Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70109/jupenkes.v3i3.136

Abstract

Minyak jelantah merupakan minyak goreng yang telah digunakan berulang kali dan dapat menghasilkan senyawa peroksida, radikal bebas, serta senyawa toksik akibat pemanasan. Pembuangan minyak jelantah secara sembarangan juga berpotensi mencemari lingkungan. Salah satu upaya pemanfaatannya adalah mengolah minyak jelantah menjadi sabun melalui reaksi saponifikasi. Kegiatan pengabdian masyarakat dilaksanakan di Desa Ngombakan, Polokarto, Kabupaten Sukoharjo, dengan tema pemanfaatan minyak jelantah sebagai sabun cuci tangan antiseptik berbahan ekstrak serai untuk mendukung pelestarian lingkungan dan pencegahan resistensi bakteri. Kegiatan diawali dengan penyuluhan mengenai pengolahan minyak jelantah, manfaat ekstrak serai sebagai antibakteri, serta edukasi pemeriksaan laboratorium dan deteksi molekuler. Evaluasi dilakukan melalui pretest dan posttest untuk mengetahui peningkatan pengetahuan peserta. Sebanyak 29 kader PKK mengikuti kegiatan dari target 40 peserta. Hasil evaluasi menunjukkan 95% peserta mengalami peningkatan nilai, dengan rata-rata pretest sebesar 80,34 meningkat menjadi 89,66 pada posttest, atau meningkat 9,31 poin. Analisis statistik menunjukkan terdapat perbedaan signifikan antara nilai pretest dan posttest (p < 0,05), sehingga penyuluhan dinilai efektif meningkatkan pengetahuan peserta. Antusiasme peserta juga terlihat dari diskusi aktif mengenai tahapan pembuatan sabun, fungsi bahan, penggunaan APD, dan pengukuran pH. Peserta memperoleh video tutorial serta contoh produk sabun sebagai tindak lanjut kegiatan.
Co-Authors Ahmad Akmal Maulana Anik Puspita Ningrum Antik Murwani Chris Srikandini Damar Ajeng Priyandari Dwi Haryatmi Elfira Dwi Novi Nastiti Erlintang Surya Shalesa Fadhila Saffa Nur Ramadhani Fitria Diniah Janah Sayekti Francisca Pingky Artha Mevia livana ph Marchamtia Sarah Nur Awalia Fajari Mastuti Widi Lestari Nabiilah Nuur Ainii Heryanti Prasasti Kusumaning Paramastri Rachma Aulia Putri Restu Ardhiyanto Retno Listyowati Reza Yuliana Sulasmi Sulasmi Sutriadi Tri Harningsih Vector Stephen Dewangga Zuzun Farida