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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Indonesian Tourism and Development Studies Agrovigor: Jurnal Agroekoteknologi Biotropika Jurnal Teknologi Pertanian AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya JURNAL BIOLOGI INDONESIA Jurnal Ilmiah Pertanian BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Jurnal Biotek
Lia Hapsari
Purwodadi Botanic Garden
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Social Sciences Veterinary

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Identification and Genetic Diversity of Pineapple Local Accessions from Kediri and Closely Related Species (Bromeliaceae) Based on matK Gene Rasyadan Taufiq Probojati; Hadiyanti, Nugraheni; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 12 No. 3 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.03.01

Abstract

Ananas comosus L. belongs to the Bromeliaceae family and has around eight subfamilies. Accessions of local pineapple have spread widely in several areas, one of which is in Kediri. However, some accessions and closely related species (Bromeliaceae) likely remain poorly understood relationships. The highly diverse morphological characters and low levels of sequence divergence in Bromeliaceae have been problematic in resolving the reconstruction of phylogenetic relationships. This study aimed to evaluate the efficiency of the matK gene as a DNA barcode to determine genetic diversity and reconstruct a phylogenetic tree in local pineapple and closely related species (Bromeliaceae). A total of 15 specimens were used in this research, of which 7 were local pineapple accessions from Kediri and eight were closely related species from the Bromeliaceae family. Whole genome DNA was isolated using a Tiangen kit and amplified using the polymerase chain reaction (PCR) technique using a specific primer. The results showed that matK was easily amplified with a DNA fragment length of 750-800 bp. The average composition value of each base was 36.9% T (U) bases, 30.1% in A bases, 15.9% in G bases, and 17.1% in C. BLAST analysis of the sequences showed that all samples were confirmed and identified according to the species names spread across local pineapple production areas (with query cover of 96-98%). However, the species names registered in the PBG database were confirmed to be different species but still in the same genus, i.e., samples B9-B14. The phylogenetic relationship analysis distinguished each accession into two large clades according to sub-families, i.e., Bromelioideae and Pitcairnioideae. This research shows that the matK gene is suitable and recommended as a DNA barcode for local pineapple and closely related species.
ANALYSIS OF MATURING AND MATURATION OF BURAHOL PURWODADI BOTANICAL GARDEN COLLECTION Ramadhan, Riski Jefri; Nareswari, Aptika Hana Prastiwi; Hadiyanti, Nugraheni; Hapsari, Lia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 2 (2025): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i2.11299

Abstract

Fruit maturing enhances maturity and quality, making fruits more attractive. This process involves physiological and biochemical changes, including increased respiration, ethylene production, texture alteration, and heat improvement. This study examines the effects of different ethylene sources: calcium carbide (CaC2), 500 mg vitamin C, and potassium permanganate (PK), on the maturing of Burahol fruit (Stelechocarpus burahol). A Completely Randomized Factiorial Design (CRFD) with five replications was employed. Treatments included E1 (control, no ethylene), E2 (0.5% CaC2 of fruit weight wrapped in cloth), E3 (500 mg vitamin C/kg fruit), and E4 (PK crystals in a sealed chamber). Data collected on day three evaluated skin color (RHS charts), texture, and mucus presence. Results showed CaC2 and PK induced optimal maturing, yielding uniformly brownish-yellow fruits (20A/Brilliant Yellow), firm texture, and no mucus. Vitamin C fails to prevent decay due to mechanical damage from forced harvesting. This study highlights CaC2 and PK as effective maturing agents, providing practical solutions for Burahol fruit post-harvest management.
Assessment for Identification of Stelechocarpus burahol and Sister Species Complex of Annonaceae Family Using trnL Intron Sequences Probojati, Rasyadan Taufiq; Hadiyanti, Nugraheni; Hapsari, Lia
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5777

Abstract

Stelechocarpus burahol (kepel) belongs to the Annonaceae family, and is considered to be a native species to Indonesia which is mainly distributed on the island of Java. However, the plant’s existence is currently difficult to find, so it is categorized as rare in Indonesia. A molecular approach using DNA barcoding technique is significant to assist plant identification. The gene that is widely used and proven to be accurate for Annonaceae is trnL-F. This study was aimed to evaluate the efficiency of trnL as DNA barcode for the identification of S. burahol and its relatives (Annonaceae) from Java Island. In total 10 specimens have been used in this study. Whole genome DNA was isolated by Tiangen kit and amplified by Polymerase Chain Reaction (PCR) technique using a specific primer. Results showed that trnL was easily amplified with a DNA fragment length of 500-600 bp. The trnL amplicons have successfully sequenced as indicated by the high QV20+ values. The sequence compositions were high in AT bases (63.9%). BLAST analysis of the sequences showed that S. burahol and sister species have been confirmed its identity according to the reference sequences in NCBI with query cover identities 98%-100%. This research can be concluded that trnL-F is suitable and recommended as a DNA barcode for S. burahol and its relatives. However, further research is suggested to combine analysis of both coding (rbcL, matK, etc) and non-coding (trnL) markers for better identification results. 
Variation and clustering analysis of several species of soursop family (Annonaceae) based on vegetative morphology characters Santoso, Yusuf; Probojati, Rasyadan Taufiq; Hapsari, Lia; Supandji, Supandji; Kustiani, Edy
Agrovigor Vol 17, No 1 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i1.24845

Abstract

The Annonaceae family exhibited a high level of diversity and is spread over many regions. The designation of the names of several species of the Annonaceae family varies in each region.  Therefore, characterization is needed to confirm and classify the taxonomy position of the species. This study aims to characterize the variation and analyze the clustering pattern of several species of the Annonaceae based on vegetative morphology characters. The type of method used in this research is exploratory descriptive, with seventeen specimen species consisting of ten Annonaceae studied in this research. Characters observed include tree age, stem diameter, plant height, stem color, type of sapling, type of branching, leaf shape, leaf tip shape, leaf base shape, leaf width, leaf length, leaf thickness, petiole length and thickness, leaf hairs , color of young and mature leaves, type of leaf edge, number of leaf veins, color of young branches, leaf hairs on young branches, and number of nodes on the stem per meter. These qualitative and quantitative characters were observed using Descriptors for Cherimoya (Annona cherimola Mill.) from Bioversity International and CHERLA. Data analysis was conducted in the Paleontological Statistics program with cluster analysis method (Bray-curtis similarity index) to produce a dendrogram topology. Results showed that there were variations in vegetative morphological characteristics among the ten species examined. Dendrogram topology was divided into 3 groups relatively following the tribe division, with similarity index 0.64 to 0.96. Xylopia sp. was separated from the others in Group 1 (Xylopiae). Group 2 (Uvariae) consisted of Fissistigma sp., Desmos chinensis, Artabotrys suaveolens, Uvaria purpurea, and Desmos sp. Group 3 (Annoneae) consisted of Annona muricata, Annona montana, Stelechocarpus burahol, and Annona glabra. Vegetative morphological characters have proven to be useful and effective in differentiating species in the Annonaceae family; it is recommended for initial identification of species, especially in the Annonoideae subfamily.
Phenotypic Variation and RAPD Polymorphism of Pisang Kepok Local Cultivars (Musa acuminata x Musa balbisiana, ABB, Saba Subgroup) Wahyudi, Didik; Ilmi, Zahrobatul Lil; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.03

Abstract

Pisang Kepok is a major local banana cultivar in Indonesia with high economic, social and cultural value. Particularly on the island of Java, there are several variations of Pisang Kepok recognised with their own local names, which makes difficulties in taxonomic identification and grouping. Morphological features are used in conventional banana cultivar classification, but they are deemed less precise due to their subjectivity, thus, it is supposed to be complemented with a molecular approach. This study aims to identify the phenotypic variation of Pisang Kepok local cultivars also their genetic polymorphism using Random Amplified Polymorphic DNA marker. Phenotypic variation was observed using 35 morphological characters. Six RAPD primers were used, i.e. OPA2, OPA3, OPA4, OPA11, OPA12, and OPA18. Clustering analysis, both phenotypic and genetic were performed using PAST v4.02. The morphological characterisation identified four variants of Pisang Kepok i.e. Kepok Abang, Kepok Putih, Kepok Manurun, and Kepok Australi; which all confirmed as ABB genome group. Phenotypic clustering showed that Pisang Kepok cultivars were separated into 3 clusters based on their local name, with a high similarity value of >90%. PCA biplot showed that the fruit flesh colour was the most important character contributed to the cultivar variation. RAPD marker also showed that each specimen was grouped according to its local name and source, with a similarity value of >80%. Both morphology and molecular (RAPD) markers resulted in the branching of Pisang Kepok which was closer to Pisang Klutuk than Pisang Barlin.
BIOMASS AND CARBON STOCK ESTIMATION INVENTORY OF INDONESIAN BANANAS (Musa spp.) AND ITS POTENTIAL ROLE FOR LAND REHABILITATION Danarto, Setyawan Agung; Hapsari, Lia
BIOTROPIA Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.824 KB) | DOI: 10.11598/btb.2015.22.2.376

Abstract

Bananas (Musa spp.) are widely cultivated in Indonesia. They are commonly grown in backyards, home gardens, intercropped with short-term crops, and in agroforestry systems. The potential of bananas to sequester carbon has been reported, but information on performance variation among cultivars remains limited. An inventory of biomass and carbon stock (C-stock) estimation was conducted on banana accessions in the Musa germplasm collection of Purwodadi Botanic Garden, Pasuruan. Biomass and C-stock estimations were carried out for 42 individual banana accessions consisting of five wild species and 37 cultivars using a non-destructive allometric equation method for banana. The objectives of this study were to estimate the biomass and C-stock of Indonesian bananas in the Purwodadi germplasm collection, to project time-average above-ground C-stock of banana-based farming systems in Indonesia, and to discuss the role of bananas in landscape rehabilitation. The results showed that biomass and C-stock values varied among accessions. Wild banana species Musa balbisiana had higher biomass and C-stock compared to Musa acuminata. Cultivars containing one or two “B” genomes (ABB and AAB) were more vigorous and contributed higher biomass and C-stock than AAA and AA cultivars. Among the cultivars, the highest C-stock contribution was recorded in Pisang Kepok Bung (average 6.92 kg C/plant), while the lowest was found in Pisang Rayap (average 0.67 kg C/plant). On average, the Indonesian bananas examined contributed approximately 2.26 kg C/plant or 0.98 tonnes C/ha. The total banana growing area increased from 73,539 ha in 2000 to 101,822 ha in 2010 and slightly decreased to 100,600 ha in 2014. These areas contributed an estimated C-stock of 72.28 tonnes (2000), increasing to 100.07 tonnes (2010), followed by a slight decline to 98.97 tonnes in 2014—values based only on recorded cultivation areas. Banana plants—especially when combined with woody tree crops—have potential as key components of agroforestry and mixed farming systems, home gardens, and landscape rehabilitation, contributing to carbon reduction and providing economic benefits for surrounding communities.
DIVERSITY AND ECOLOGY OF UNDERSTORY PLANT IN SEMPU ISLAND, EAST JAVA, INDONESIA Hapsari, Lia; Abywijaya, Ilham Kurnia; Nurfadilah, Siti; Rindyastuti, Ridesti
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.1145

Abstract

As an indicator of environmental disturbances, the understory is an important structural and functional component of forest ecosystems. Hence, this study was conducted to assess the diversity and composition of understory plants in the lowland forests adjacent to the trekking paths along Semut Bay (dock entrance), Segara Anakan Lagoon, and several coastal areas in Sempu Island, and to determine their relationship with the underlying environmental factors as disturbance indicators through the presence of understory invasive alien species (IAS). Sixty site plots of 2 × 2 m² were established to analyze the understory vegetation, site profiles, and environmental variables. A total of 135 understory plant species belonging to 108 genera and 60 families were recorded within the 60 plots of the study areas. Poaceae was the dominant family, followed by Compositae, Phyllanthaceae, Sterculiaceae, Rubiaceae, Putranjivaceae, and Cyperaceae. The understory communities in the lowland forest adjacent to Semut Path were dominated by tree seedlings and had a relatively lower composition of shrubs. Coastal areas, which have more open canopies, were dominated by grasses and shrubs. Some dominant native understory species included Grewia spp., Cycas rumphii, and others. Indicating disturbance, 12 understory IAS were found in the study areas. Four of these are among the world’s worst invaders (Ageratum conyzoides, Chromolaena odorata, Lantana camara, Mikania micrantha), and three are noxious weeds (Eleusine indica and Imperata cylindrica). Light intensity and air temperature were strongly and positively associated with disturbed sites, while relative humidity, soil pH, and elevation were associated with less disturbed sites. The study results provide a scientific basis and recommendations on the current diversity status of understory plant species at Sempu Island, helping justify further conservation of indigenous species and their protection from disturbances.
Inventory of Invasive Plant Species along the corridor of Kawah Ijen Nature Tourism Park, Banyuwangi, East Java Hapsari, Lia; Basith, Abdul; Novitasiah, Hari Rusdwi
Journal of Indonesian Tourism and Development Studies Vol. 2 No. 1 (2014)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2014.002.01.01

Abstract

A field survey was conducted in November 2013 to inventory invasive plant species present along the corridor of Kawah Ijen Nature Tourism Park exploratively. Result showed that there were 11 plant species found abundantly along the corridor. Typical native species were dominated by Cyathea contaminans, Casuarina junghuhniana and Vaccinium varingiaefolium. Three species were determined as invasive alien species i.e. Chromolaena odorata, Acacia decurrens and Blumea lacera whereas five species were determined as native species but potential invaders i.e. Rubus moluccanus, Melastoma malabatrichum, Polygonum barbatum, Debregeasia longifolia and Pteridium aquilinum. In term of tourism particularly on nature-based destinations enable moving in and out of invasive alien species due to opening the access of some natural protected areas. The environmental impact of an alien species whether it becomes invasive at its destination depends on its biological key point,  what ecological role the species may play, and on additional factors such as its tolerance of the gross features of the environment in the new range. Keywords: invasive plants, corridor, Kawah Ijen, Nature Tourism Park, Banyuwangi
Spatial Diversity in Composition and Structure of Nekton in Ngenep Spring and its Channels, Karangploso - Malang Hapsari, Lia; Sihombing, Maic Audo Lin; Rohie, Ade Meylisa Crystel; Retnaningdyah, Catur
Journal of Indonesian Tourism and Development Studies Vol. 2 No. 2 (2014)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitode.2014.002.02.04

Abstract

Water springs and its channel degradation due to anthropogenic pollution may alter the community structure of aquatic organisms. Water spring degradation therefore affect the quality of water as tourism resources. This study aims to investigate the changes in community structure of nekton and determine the relationships between water quality characteristics to the diversity of nekton. The field survey was set up in Ngenep spring and its channels. Results showed that nekton species found in Ngenep spring and its channels consists of 4 classes, 4 orders, 6 families, and 7 species with total 627 nekton samples. It is comprises of fishes, shrimp, frogs and waterstriders. Nekton diversity index (H') in the spring and irrigation channel were in moderate level (1<H'<3) while in settlement channel was low (0,67). Evenness values of nekton ranged 0,24 - 0,53, whereas dominancy index of nekton ranged 0,41 - 0,74. Evenness value in settlement channel was very low (0, 24) with high dominancy index (0, 74); it indicates that nekton species were spread not evenly in the channel, it dominated by fish Rasbora sp. (highest IVI, 184,95). There were spatial variations of physico-chemical water qualitiy parameters in Ngenep springs and its channels (temperature, stream velocity, turbidity, conductivity, pH, DO, BOD and TOM) which affected to nekton diversity and community structure. Clustering analyses and PCA result shows correlation pattern between nekton distribution with physico-chemical water quality parameters. However, physico-chemical water quality parameters in Ngenep springs and its channel were still optimum as nekton habitat (PP No. 82/ 2001). Keywords: Community structure, Nekton, Spatial diversity, Spring, Water channel
Co-Authors Abdul Basith Abywijaya, Ilham Kurnia Ade Meylisa Crystel Rohie Ahmad Masrum Catur Retnaningdyah Devina Cinantya Anindita Dewi Ayu Lestari Didik Wahyudi Edy Kustiani Gusmiati, Lutfiana Hasanah Hari Rusdwi Novitasiah Ilmi, Zahrobatul Lil Maic Audo Lin Sihombing Mas'udah, Shofiyatul Mudinillah, Adam Munawaroh, Putri Syepty SriJanatul Nareswari, Aptika Hana Prastiwi Nugraheni Hadiyanti Ramadhan, Riski Jefri Rasyadan Taufiq Probojati Ridesti Rindyastuti Santoso, Yusuf Saputra, Yudha Setyawan Agung Danarto Siti Nurfadilah Supandji, Supandji Trimanto Trimanto Widya Dwi Rukmi Putri