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All Journal Bioscience Microbiology Indonesia BIO-SITE |BIOLOGI Sains Terapan Jurnal Pengabdian UntukMu NegeRI Jurnal SOLMA JURNAL PHOTON Sciscitatio : Journal for Biological Science Bioscientist : Jurnal Ilmiah Biologi
ISRAWATI HARAHAP
Program Studi Biologi, Fakultas MIPA Dan Kesehatan Universitas Muhammadiyah Riau
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Physics Social Sciences Other

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Isolation of Fungal Endophytes from Melastoma malabathricum L. and Their Potential as Antifungal Rusi Octavianti; Israwati harahap; Elsie Elsie
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.976 KB)

Abstract

The aim of this study was to isolation the endophytic fungi from medicinal plant Melastoma malabathricum L. and to investigate their potential antifungal activity. Isolation endophytic fungi was carried out using surface sterilized method. The fungal extracts were assessed for antifungal activity against Candida albicans using agar well diffusion method. A total of 28 isolates fungal endophytes from leaves, stems, flowers and roots of Melastoma malabathricum L. The results showed that of twelev have strong category within inhibit growth of C. albicans. Isolate 18 is endophytic fungi who produced the largest zones of inhibit is 19 mm.
Community Structure of Sporulating Fungi on Decaying Litters of Shorea spp. ISRAWATI HARAHAP; GAYUH RAHAYU; IMAN HIDAYAT
Microbiology Indonesia Vol. 7 No. 3 (2013): September 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.158 KB) | DOI: 10.5454/mi.7.3.3

Abstract

The community structure of sporulating fungi on decaying branch and leaf litters of Shorea spp. were studied to reveal the common saprobic fungi. The study was mainly based on morphological observation. Twenty-nine species of the sporulating fungi were found on Shorea spp. litters at Situ Gede and Bubulak forest area, Bogor, West Java. The fungi included seven species of Ascomycetes (Annulohypoxylon purpureonitens, Diatrype chlorosarca, Didymosphaeria epidermidis, Lophiostoma sp.,  Lophodermium sp., Pemphidium sp., and Valsa sp.) and 22 species of anamorphic taxa that consisted of 12 Coelomycetes (Coniella musaiaensis, Coryneum betulinum, Hendersoniopsis thelebola, Lasiodiplodia theobromae, Lasmeniella guaranitica, Leptodothiorella sp., Massariothea themedae, Pestalotia guepinii, Pestalotiopsis sp., Pseudolachnea hispidula, Septoriella sp., and unidentified species of Coelomycetes) and 10 Hyphomycetes (Beltraniella portoricensis, Cryptophialoidea fasciculata, Hermatomyces spaerichus, Kiliophora ubiensis, Minimidochium setosum, Monodisma fragilis, Nodulisporium sp., Stilbella fimetaria, Virgatospora echinofibrosa, and unidentified Hyphomycetes). The  most common taxa occuring on decaying leaf litter were B. portoricensis and Pemphidium sp., while those on decaying branch material were L. theobromae and C. fasciculata. The fungal community was subtrate specific. The community on decaying branch litter was more diverse than that on leaf litter. The C/N ratio of the substrate was closely related to the structure  of the community.
ISOLASI DAN SELEKSI CENDAWAN ENDOFIT DARI TANAMAN BETADIN (Jatropha multifida L.) DAN POTENSINYA SEBAGAI ANTIMIKROBA Israwati Harahap; - Elsie; Indri Nurjanah
Sistem Informasi Vol 7 No 02 (2017): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v7i02.514

Abstract

This research aimed to isolate and select endophytic fungi that potentially as antimicrobial from betadin plants (Jatropha multifida L.). Isolation of endophytic fungi was carried out using surface sterilization and selection of endophytic fungi using well diffusion method. A total of 13 isolates of endophytic fungi were obtained from J. multifida L. From a total of 13 isolates, 11 isolates were able to inhibit the growth of Candida albicans, 11 isolate was able to inhibit the growth of Staphylococcus aureus and 10 isolates were able to inhibit the growth of Escherichia coli.
ISOLASI CENDAWAN ENDOFIT DARI AKAR WANGI (Vetiveria zizanioides L.) DAN POTENSINYA SEBAGAI ANTIMIKROBA Israwati Harahap; - Elsie
Sistem Informasi Vol 8 No 01 (2017): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v8i01.542

Abstract

Endophytic fungi are microorganisms living in healthy tissue of their host plants without causing disease. Endophytic fungi live in every plant, including Vetiveria zizanioides L. medicinal plant intracellulary and/or intercellulary. This plant was screened pharmacologically for antibacterial, antifungal, anticataleptic, analgesic and anti-inflammatory, rheumatism, anti oxidant and anti arthritic activity. This research aimed to isolation endophytic fungi from V. zizanioides L. and to screening their antimicrobial potency against microflora Escherichia coli, Staphylococcus aureus, and Candida albicans. Endophytic fungi isolation from V. zizanioides L. was done by surface sterilization method. A total of 33 isolates were recovered from 88 leaves and 88 root segments. Based on antimicrobial activitytest, most of the fungal extracts showed in vitro inhibition of microbes growth.
ISOLASI Escherichia coli PADA DAGING SAPI SEGAR YANG DIPEROLEH DARI BEBERAPA PASAR TRADISIONAL DI PEKANBARU - Elsie; Israwati Harahap
Sistem Informasi Vol 7 No 01 (2016): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v7i01.570

Abstract

Escherichia coli is a bacteria that inhabitant in the cattle or human intestine. Raw beef sold in the traditional markets is easily to contaminated by E. coli. It’s because the markets is one of the vulnerable and the risk is high enough against contamination. The goal of this research is to detect E. coli contamination in the fresh raw beef at several markets in Pekanbaru. This research used 100 gr fresh raw beef purchased from the six traditional markets in Pekanbaru. The study begins with a Most probable Number (MPN) test, than isolation and identification of the media ECB and L-EMB, initially with IMViC test (Indol, Methyl Red, Voges Praskauer and Citrate). Results of MPN test showed that all raw beef were contaminated E.coli with MPN varying value.
UJI AKTIVITAS ANTIBAKTERI DARI ISOLAT CENDAWAN ENDOFIT ASAL TUMBUHAN SENDUDUK (Melastoma malabathricum L.) TERHADAP Escherichia coli DAN Staphylococcus aureus Israwati Harahap; Vivin Paddillah Rahmi; Nofripa Herlina
Sistem Informasi Vol 8 No 2 (2018): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v8i2.741

Abstract

Endophytic fungi is a symbiotic microorganism which live inside plant tissues and not harm to their host. Several genera from endophytic fungi known to produce secondary metabolite compounds like antibiotics, anticancer, antifungal, antiviral and antimalarial. This study aimed to investigate antibacterial activity of endophytic fungi from senduduk (Melastoma malabathricum L.) against Escherichia coli and Staphylococcus aureus. Based on the test result, 11 isolates endophytic fungi from senduduk were known to have antibacterial activities. Isolate code 23 have ability to inhibit the growth of E.coli with diameters zone of inhibition is 22 mm and eight isolate endophytic fungi have ability to inhibit the growth of S.aureus (showed by inhibition zone).
UJI AKTIVITAS ANTIBAKTERI ISOLAT AKTINOMISETES ASAL TANAH HUTAN LARANGAN ADAT GHIMBO POTAI, KABUPATEN KAMPAR, PROVINSI RIAU, TERHADAP Escherichia coli DAN Staphylococcus aureus Elsie - -; Nur’Atika - -; Israwati - Harahap
Sistem Informasi Vol 9 No 1 (2018): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v9i1.1083

Abstract

Tujuan penelitian ini untuk menguji aktivitas antibakteri isolat aktinomisetes yang telah diisolasi dari tanah Hutan Larangan Adat Ghimbo Potai, Kabupaten Kampar, Provinsi Riau. Penelitian dilakukan pada bulan April sampai dengan Juli tahun 2016. Uji aktivitas dilakukan menggunakan metode difusi agar terhadap 2 bakteri uji yaitu Escherichia coli and Staphylococcus aureus. Hasil uji aktivitas isolat aktinomisetes NA-1 dan NA-2 menunjukkan bahwa hanya isolat NA-1 yang memiliki kemampuan menghambat pertumbuhan bakteri S. aureus dengan diameter zona penghambatan sebesar 7 mm.
Antibacterial Activity of Ethanol Extract of Kale Leaves (Brassica oleracea var. sabellica) against Escherichia coli and Staphylococcus aureus Elsie Makmur; Mifta Hunnazah; Israwati Harahap
Sistem Informasi Vol 12 No 1 (2021): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v12i1.3347

Abstract

Penggunaan antibiotik yang kurang tepat dapat menimbulkan masalah resistensi pada bakteri patogen. Salah satu alternatif untuk menanggulangi hal tersebut adalah dengan pencarian senyawa antibakteri dengan memanfaatkan ekstrak tanaman, salah satunya adalah kale. Penelitian ini bertujuan untuk mengetahui potensi ekstrak daun kale sebagai sumber senyawa antibakteri terhadap Escherichia coli dan Staphylococcus aureus, serta mengetahui konsentrasi terbaik yang mampu menghambat pertumbuhan E. coli dan S. aureus sehingga dapat menentukan nilai Minimum Inhibitory Concentration (MIC) ekstrak daun kale. Ekstrak daun kale diperoleh melalui metode maserasi sedangkan uji aktivitas antibakteri dilakukan dengan metode difusi agar (Kirby Bauer). MIC terhadap E. coli dan S. aureus ditentukan dengan metode dilusi cair. Ekstrak daun Kale konsentrasi 140 mg/mL berpotensi sebagai sumber senyawa antibakteri dengan terbentuknya zona hambat terbesar pada E. coli sebesar 3,3 mm dan sebesar 3,93 mm pada S. Aureus. Sementara itu, nilai MIC terhadap E. coli diperoleh dari konsentrasi 50% sedangkan terhadap S. aureus diperoleh dari konsentrasi ekstrak Kale 25%, masing-masing dari pengenceran konsentrasi aktivitas antibakteri 140 mg/mL.
Pemeriksaan Golongan Darah bagi Guru Biologi SMAN dalam Rangka Peningkatan Kualitas Guru Israwati harahap; Novia Gesriantuti; Nofripa Herlina; Elsie Elsie; Yeeri Badrun
Jurnal SOLMA Vol. 7 No. 2 (2018)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.336 KB) | DOI: 10.29405/solma.v7i2.1176

Abstract

Kegiatan Pengabdian kepada Masyarakat yang telah dilakukan ini bermula dari adanya keinginan untuk memberikan keterampilan yang dapat meningkatkan kompetensi guru-guru biologi. Hasil wawancara tim pelaksana pengabdian kepada Masyarakat Biologi UMRI dengan guru biologi SMAN 14 Pekanbaru, menunjukkan bahwa guru-guru Biologi SMAN 14 Pekanbaru memiliki keterbatasan ilmu dalam materi golongan darah dan tidak memiliki keterampilan dalam melakukan pemeriksaan golongan darah. Untuk itu, dengan adanya pelatihan pemeriksaan golongan darah ini, guru-guru biologi SMAN 14 Pekanbaru dapat memberikan pelajaran tentang materi golongan darah serta praktik pemeriksaan golongan darah kepada siswa. Kegiatan pelatihan ini dilaksanakan pada tanggal 1 Februari 2018. Pada saat praktik pemeriksaan golongan darah, guru-guru biologi sangat antusias mengikuti pelatihan dan mereka berharap adanya pelatihan lain yang dapat meningkatkan kompetensi para guru.
Potensi Bakteri Endofit Asal Tumbuhan Katemas (Euphorbia heterophylla L.) sebagai Pengendali Bakteri Penyebab Penyakit Busuk Lunak (Erwinia chrysanthemi) Nurhakiki Nurhakiki; Elsie Elsie; Israwati Harahap
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 1 (2022): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i1.5083

Abstract

Disease control in plants is generally controlled chemically using chemicals, but it can have a negative impact. One alternative to control plant diseases is to use microbial biological agents, namely endophytic bacteria. Endophytic bacteria from Euphorbia heterophylla L.Can be used as a controller for soft rot disease caused by Erwinia chrysanthemi. The purpose of this study was to isolate and determine the potential of endophytic bacteria from the katemas plant as a controller for soft rot disease caused by E. chrysanthemi. This study uses an experimental method. Isolation of antibacterial endophytic bacteria using surface sterilization method. Activity tests were carried out using the Kirby-Bauer method (disk diffusion). The parameter studied was to measure the inhibition zone formed around the paper disc using a caliper. The results of this study obtained 5 isolates of endophytic bacteria from the katemas plant which may control soft rot disease by E. chrysanthemi. Based on the research that has been done, it can be said that the results of the isolation of endophytic bacteria from the katemas plant obtained 5 isolates of endophytic bacteria with the inhibitory zone diameter of NK 1, NK 2, NK 3, NK 4 and NK 5 isolates of 1.1 mm each; 3.8mm; 2.0mm; 1.5mm and 22.5mm.
Co-Authors -, Nur’Atika - -, Yeeri Badrun Delovita Ginting, Delovita Elsie Elsie Elsie Elsie Gayuh Rahayu IMAN HIDAYAT Indri Nurjanah Ivan Permana Putra Laras Sita Widara Mahardhika, Wahyu Aji Mifta Hunnazah Neneng Fitrya Nofripa Herlina Nopripa Herlina Novia Gesriantuti Nurhakiki Nurhakiki Nurjanah, Indri Nur’Atika - - Rahmi, Vivin Paddillah Romi Fadli Syahputra Rusi Octavianti Shabri Putra Wirman Sri Fitria Retnawaty Sri Mulyani Vivi Novrianti Viviana Lubis Vivin Paddillah Rahmi Yeeri Badrun Yulia Fitri