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Resistance Induction of Cocoa Seeds Against Fruit Rot Disease (Phytophthora palmivora Butl.) Using Rhizobacteria-Threated Seed Deby Rezita; Syamsuddin Syamsuddin; Hasanuddin Hasanuddin
Jurnal Agrista Vol 25, No 2 (2021): Volume 25 Nomor 2 Agustus 2021
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Penelitian ini bertujuan untuk mengetahui kemampuan rizobakteri yang diberikan melalui perlakuan benih mempengaruhi induksi ketahanan bibit kakao terhadap penyakit busuk buah (Phytophthora palmivora Butl.). Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih, Jurusan Agroteknologi, Fakultas Pertanian Universitas Syiah Kuala, Darussalam Banda Aceh sejak September 2020 sampai Februari 2021. Rancangan yang digunakan ialah Rancangan Acak Lengkap (RAL) non faktorial dengan 7 perlakuan yang diulang sebanyak 3 kali sehingga diperoleh 21 unit perlakuan. Peubah yang diamati ialah persentase penghambatan pertumbuhan koloni patogen, laju penghambatan pertumbuhan koloni patogen, pengukuran aktivitas peroksidase, potensi tumbuh maksimum, daya berkecambah, keserempakan tumbuh, kecepatan tumbuh relatif, waktu yang dibutuhkan untuk mencapai 50% perkecambahan total relatif, tinggi tanaman, diameter batang dan jumlah daun. Hasil penelitian ini menunjukkan bahwa rizobakteri berpengaruh sangat nyata terhadap persentase penghambatan pertumbuhan koloni patogen, laju penghambatan pertumbuhan koloni patogen, pengukuran aktivitas peroksidase, daya berkecambah, kecepatan tumbuh relatif , tinggi tanaman bibit kakao umur 28 HST, tinggi tanaman bibit kakao umur 35 HST, diameter batang bibit kakao umur 28 HST, diameter batang bibit kakao umur 35 HST dan jumlah daun bibit kakao umur 28. HST. Resistance Induction of Cocoa Seeds Against Fruit Rot Disease (Phytophthora palmivora Butl.) Using Rhizobacteria-Threated Seed This study aimed to determine the ability of rhizobacteria given through seed treatment to influence the induction of resistance of cocoa seedlings to fruit rot disease (Phytophthora palmivora Butl. ). This research was conducted at the Seed Science and Technology Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh from September 2020 to February 2021. The design used was a non-factorial Completely Randomized Design (CRD) with 7 treatments and 3 times replications for experiments, so there were 21 experimental units. The variables observed were the percentage of inhibition of pathogenic colony growth, rate of inhibition of pathogenic colony growth, measurement of peroxidase activity, maximum growth potential, germination, growth simultaneity, relative growth rate, the required time to reach 50% of relative germination, plant height, stem diameter and number of leaves. The results of this study showed that rhizobacteria had a very significant effect on the percentage of inhibition of pathogenic colony growth, rate of inhibition of pathogenic colony growth, measurement of peroxidase activity, germination, relative growth rate, height of cacao seedlings 28 DAP, height of cacao seedlings 35 DAP, stem diameter of cacao seedlings 28 DAP, stem diameter of cacao seedlings 35 DAP and numer of leaves of cacao seedlings 28 DAP.

PENGARUH PERLAKUAN BENIH MENGGUNAKAN RIZOBAKTERI PEMACU PERTUMBUHAN TANAMAN TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN OKRA (Abelmoschus Esculentus L. Moench) Putri Alfarrah Dhifa; Syamsuddin Syamsuddin; Hasanuddin Hasanuddin
Jurnal Agrista Vol 25, No 2 (2021): Volume 25 Nomor 2 Agustus 2021
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan benih menggunakan rizobakteri pemacu pertumbuhan tanaman dan jenis rizobakteri yang tepat terhadap peningkatan pertumbuhan dan produksi tanaman okra. Penelitian ini dilaksanakan di Kebun Percobaan 1 dan Laboratorium Ilmu dan Teknologi Benih Fakultas Pertanian Universitas Syiah Kuala yang berlangsung pada bulan Oktober sampai Desember 2020. Percobaan disusun berdasarkan rancangan acak kelompok (RAK) non faktorial dengan 16 perlakuan yang terdiri atas 15 perlakuan isolat rizobakteri dan perlakuan kontrol. Setiap perlakuan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwa perlakuan terbaik terdapat pada jenis rizobakteri Bacillus badius yang mampu meningkatkan parameter tinggi tanaman okra 15, 30, 45 dan 60 HST dan jenis rizobakteri Bacillus larvae yang mampu meningkatkan diameter batang 60 HST, jumlah daun 45 dan 60 HST serta berat buah pertanaman. The Effect of Seed Treatment Using Plant Growth Promoting Rhizobacteria on Growth and Production of Okra (Abelmoschus esculentus L. Moench) This study aims to determine the effect of seed treatment using plant growth promoting rhizobacteria and the right types of rhizobacteria on increasing growth and production of okra. This research was conducted at Experimental Garden 1 and Seed Science and Technology Laboratory, Faculty of Agriculture, Syiah Kuala University from October up to December 2020. Randomized Block Design (RBD) non factorial that consisted 16 treatments was used in the research. The treatments were control and 15 rhizobacteria isolates, every treatment was replicated 3 times. The results showed that the best seed treatment using rhizobacterial isolates Bacillus badius was able to increase the height parameters of okra plants 15, 30, 45 and 60 DAP, and rhizobacteria of Bacillus larvae were able to increase stem diameter 60 DAP, number of leaves 45 and 60 DAP and weight of fruit in a plant.

Pengaruh Pemberian Nitrogen dan Boron Melalui Daun terhadap Mutu Benih Kedelai (Glycine max (L.) Merril ) Julita HD; Syamsuddin Syamsuddin; Rita Hayati
Jurnal Floratek Vol 11, No 1 (2016): April 2016
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Effect of Foliar Application of Nitrogen and Boron on Quality of Soybean Seed (Glycine max (L.) Merril)ABSTRACT. The aim of this research was to investigate the effect of foliar application of nitrogen and boron on quality of soybean seed (Glycine max (L.) Merril). The research was conducted at Balai Pengkajian Teknologi Pertanian (BPTP) Aceh, Seed Laboratory of Agriculture Faculty, Syiah Kuala University and Center for Biological Resources and Biotechnology, Institute for Research and Community Empowerment, Bogor Agricultural Institute which is started from June 2013 to February 2014. This research was carried experimentally using a randomized block design with three replication, consist of two factor. The first factor was nitrogen concentration witch consisted of four levels: 0, 0,2, 0,4 and 0,6%. The second factor was boron dossages ie : 0, 0,35, 0,45, 0,55 kg B ha-1. The results showed that nitrogen concentration have very significant effect on germination, the rate of seed growth, seed growth simultaneity and significant effect on seed protein content. Best nitrogen concentration level found in the use of 0,6% N. Boron have very significant effect on germination, seed growth simultaneity and significantly effect on the rate of seed growth. The best boron level was found at a dose of 0,45 kg B ha-1. The combination of boron 0,6% N and 0% gives a higher yield for the germination, the rate of the seed growth, seed growth simultaneity and protein, which is not significantly different from its value with the addition of boron 0,35, 0,45 and 0,55 kg B ha-1.

Performansi Genotip Padi Beras Merah dari Varietas Lokal Aceh yang Dibudidayakan secara Aerobik pada Sistem SRI Organik Efendi Efendi; Syamsuddin Syamsuddin; Betti Agustina
Jurnal Floratek Vol 11, No 1 (2016): April 2016
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Performance of Red Rice Genotype from Aceh’s Province that Cultivated with Aerobic System in Organic SRI ABSTRACT. Characterization of the performance on morpho-agronomic brown rice originating from local varieties of Aceh has been done in order to develop new varieties that adapt to climate change and suitable for organic SRI method. Eighteen red rice genotypes have been planted to evaluate the characters based on the method of cultivation in aerobic system. The red rice genotypes were observed and showed the variation in very significant to the formation of tillers, plant height, number of panicles, panicle length, grain weight per plant, 1000 grain weight, and grain yield potential. The results showed that the genotypes studied had high genetic diversity. Tillering in red rice varies between 19-30 days after sowing. The highest plant height found in genotype Pade Mirah 44, while the lowest plant found in genotype Sipulo Mirah. It was dicovered that the number of panicle significantly affected by the red rice genotype. Number of red rice panicle ranged between 14-48 per plant. The number of panicles in genotype Ketan Mirah was higher than genotype of Meuligai Mirah. However, Ketan Mirah 2 had the shortest panicles. Grain weight per panicle significantly also affected by red rice genotypes. The highest grain weight found in genotype Pineung Lango 172. In addition, also found that the weight of 1000 grain red rice was significantly different between the genotypes. The smallest size of a grain found in genotype of Sirias 102, whereas genotype Pade Mas 41 showed the largest size of grain (30.8 g). Research showed that genotypes affect grain yield potential significantly. The highest yield potential obtained from the genotype Pineung Lango 172 (6.8 ton/ha). In contrast, the lowest result was found in genotype Sipulo Mirah (2.8 tons/ha). Therefore, the results of the present invention is expected to provide information to enhance the sustainability of rice plant breeding system of organic red rice according to SRI system.

Pengaruh Tingkat Kekerasan Buah dan Letak Benih dalam Buah terhadap Viabilitas dan Vigor Benih Kakao (Theobroma cacao L.) Nanda Fadila; Syamsuddin Syamsuddin; Rita Hayati
Jurnal Floratek Vol 11, No 1 (2016): April 2016
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Effect of the Pod Hardness Level and Seed Position in Pod on Cocoa Seed (Theobroma cacao L.) Viability and Vigor ABSTRACT. The study was aimed to recognize the effect of the pod hardness level, seed position in pod, and the interaction between them on cocoa seed viability and vigor. The design used was a factorial completely randomized design (CRD) with 3 replicates. The level of pod hardness as the first factor consisted of 4 levels: 3.5 kg/second, 3.0 K ≤ 3.5 kg/second, 2.5 K ≤ 3.0 kg/second and 2.0 K ≤ 2.5 kg/second. The position of seed in pod as the second factor covers 3 levels, they are both ends and the middle of the pod. Observation conducted to the seed dried weight (BK), maximum growth potency (PTM), germination capacity (DB), vigor index (IV), growth speed (KCT), simultaneity growing (KST), time needed to reach 50% from the total of relative germination (T50) and dry weight of normal sprouts (BKKN). The results showed that the highest viability and vigor of cocoa seed was found from 2.5 K ≤ 3.0 kg/second of pod hardness level. The highest viability and vigor of cocoa seed came from the middle of the pod. The best combination was found from 2.5 K ≤ 3.0 kg/second of pod hardness level in the middle of the pod.

Perlakuan Benih Menggunakan Rizobakteri Pemacu Pertumbuhan terhadap Pertumbuhan Vegetatif dan Hasil Tanaman Cabai Merah (Capsicum annuum L.) Mardiah Mardiah; Syamsuddin Syamsuddin; Efendi Efendi
Jurnal Floratek Vol 11, No 1 (2016): April 2016
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Seed Treatment Using Rhizobactery Growth Promoters to Vegetative Growth and Yield of Red Chili Plant (Capsicum annum, L.) ABSTRACT. This research aims to determine the effect of seed treatment using plant growth promoter rhizobactery to vegetative growth and yield of two varieties of red chili in the field. The experiment using factorial randomized block design. Factors under study consists of varieties chili PM888 and PM999. Meanwhile, type of rhizobactery consists of Bacillus lichiniformis, Necercia sp., Actinobacter sp., Bacillus larvae, and Pseudomonas capacia. Seed as control is not treated with rhizobactery. Each treatment combination was replicated 3 times. Each experimental unit represented by 5 sample plants. Observed parameters comprise of vegetative growth and reproductive parameters. Result data from observation were analyzed using ANOVA, followed by different test between treatment with Honestly Significant Difference test on the significant level α = 0,05. The results showed that PM888 variety is superior compared with PM999 respect to the response of these varieties for seed treatment with rhizobactery based on the evaluation of the parameters of vegetative growth. While based on production parameters, the number of fruit per plant, PM888 variety significantly more fruit if seed treated using rhizobactery isolate of P. capacia . Meanwhile, rhizobactery species that effectively increase the number of fruit per plant in PM999 variety is P.capacia and P. dimuta. Result of weight measurement of fruit per plant showed that almost all rhizobacteries which effectively increase weight of fruit per plant PM888 variety, except rhizobactery of species Actinobacter sp. As for PM999 variety, there are 4 species rhizobactery which effectively increase the weight of fruit per plant, namely P. capacia , P. dimuta , Necercia sp , and Flavobacterium sp.

Uji In Vitro Isolat Rizobakteri Asal Pidie terhadap Penghambatan Pertumbuhan Phytophthora capsici dan sebagai Pemacu Pertumbuhan Tanaman Siti Sarah Afifah; Halimursyadah Halimursyadah; Syamsuddin Syamsuddin
Jurnal Ilmiah Mahasiswa Pertanian Vol 8, No 2 (2023): Mei 2023
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak. Penelitian ini bermaksud untuk mengetahui jenis rizobakteri dari rizosfer tanaman cabai asal Kecamatan Simpang Tiga, Kabupaten Pidie yang berpengaruh terhadap pertumbuhan patogen Phythopthora capsici dan sebagai rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian dilangsungkan di Laboratorium Ilmu dan Teknologi Benih Fakultas Pertanian dan Laboratorium Biologi Fakultas Keguruan dan Ilmu Pendidikan Universitas Syiah Kuala. Pelaksanaan penelitian dimulai dari Mei 2022 sampai Desember 2022. Rancangan yang digunakan ialah Rancangan Acak Lengkap (RAL) non faktorial terdiri dari 9 taraf perlakuan rizobakteri dan diulang sebanyak tiga kali pengulangan. Adapun peubah yang diamati terdiri dari persentase penghambatan pertumbuhan koloni patogen, laju penghambatan pertumbuhan koloni patogen, potensi rizobakteri memproduksi Hidrogensianida (HCN), potensi rizobakteri memproduksi IAA dan melarutkan fosfat. Persentase penghambatan rizobakteri tertinggi diamati pada LL 7/3 dan Laju penghambatan tumbuh koloni patogen tertinggi ditunjukkan isolat rizobakteri LL 6/2 dan LL 8/3 dan laju pertumbuhan terendah ditunjukkan isolat LL 7/3. Dari 9 isolat yang diuji hanya isolat LL 6/3 yang berpotensi memproduksi HCN dengan kadar HCN sedang. Seluruh isolat rizobakteri mampu menghasilkan IAA dengan rentang 0,021 µg/mL – 0,832 µg/mL. Seluruh isolate yang diuji mampu melarutkan fosfat.In Vitro Test of Rhizobacterial Isolates from Pidie Regency on Growth Inhibition of Phytophthora capsici and as Plant Growth PromoterAbstract. This study aimed to discover the effect of the type of rhizobacteria from the rhizosphere of chili plants from Simpang tiga District, Pidie Regency which affect the growth of pathogenic colonies of Phythopthora capsici and as plant growth promoting rhizobacteria (PGPR).This research was directed at the Laboratory of Seed Science and Technology, Faculty of Agriculture and Biology Laboratory, Faculty of Teaching and Education, Syiah Kuala University. The research started from May 2022 to December 2022. The design used was a non-factorial Completely Randomized Design (CRD) with a factor consisting 9 levels of rhizobacterial treatment and repeated three times. The parameters examined were the percentage of inhibition of pathogen growth, the rate of inhibition of pathogen growth, the ability of rhizobacteria to produce IAA and dissolve phosphate. The highest percentage of rhizobacterial inhibition was found in isolate LL 7/3 and the highest rate of inhibition of colony growth detected in isolate LL 6/2 and LL 8/3, the lowest rate was found in isolate LL 7/3. Only isolate LL 6/3 were able to produce HCN with moderate HCN levels of 9 isolates of rhizobacteria tested. All rhizobacterial isolates were capable to produce IAA with a range of 0,021 µg/mL – 0,832 µg/mL. All examined rhizobacterial isolates were capable to dissolve phosphate.

Uji rizobakteri agen biokontrol terhadap Rigidosporus microporus pada Tanaman Pala (Myristica fragrans) secara in vitro Putri Khairanisyah; Tjut Chamzurni; Syamsuddin Syamsuddin
Jurnal Ilmiah Mahasiswa Pertanian Vol 3, No 4 (2018): November 2018
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh jenis rizobakteri yang berpotensi sebagai agen biokontrol terhadap daya hambat patogen Rigidoporus microporus pada tanaman pala secara in vitro yang dilakukan pada Laboratorium Ilmu dan Teknologi Benih Fakultas Pertanian Universitas Syiah Kuala mulai bulan Maret sampai Mei 2018. Sampel yang digunakan yaitu patogen R. microporus penyebab jamur akar putih (JAP) pada tanaman pala dan isolat rizobakteri dari tanaman yang sehat diantara tanaman yang sakit. Hasil yang diperoleh dari penelitian ini yaitu terdapat 44 isolat rizobakteri murni, setelah diuji daya hambat terdapat 27 isolat rizobakteri yang dapat menghambat patogen R. microporus dan setelah uji antagonisme terdapat 18 isolat rizobakteri yang mampu menekan pertumbuhan koloni patogen R. microporus. Isolat rizobakteri yang memiliki penghambatan paling tinggi terdapat pada AP 5/7 dengan penghambatan 74.44% yang memiliki ciri-ciri bentuk permukaan koloni cembung, berwarna putih, dengan pinggiran koloni tidak rata dan AP 8/2 dengan penghambatan 61.11% yang memiliki ciri-ciri bentuk permukaan koloni rata, berwarna putih, dengan pinggiran koloni tidak rata.Rhizobacterial test of bio control agent toward Rigidosporus microporus on Nutmeg Plants (Myristica fragrans) in vitroAbstract. This study aims to determine the effect of rhizobacteria types that have potential as bio control agents on the pathogenic inhibitory power of Rigidoporus microporus on nutmeg plants in vitro which was conducted at the Laboratory of Seed Science and Technology, Faculty of Agriculture, Syiah Kuala University, from March to May 2018. The samples used were pathogenic R. microporus causing white root fungus (JAP) on nutmeg plants and rhizobacterial isolates from healthy plants among diseased plants. The results obtained from this study is there were 44 pure rhizobacterial isolates, after testing the inhibitory power, there were 27 isolates of rhizobacteria that could inhibit R. microporus pathogens and after antagonism test there were 18 isolates of rhizobacteria that could suppress the growth of R. microporus pathogenic colonies. Rhizobacterial isolates with the highest inhibition were found on AP 5/7 with 74.44% inhibition which had features of convex, white colony surface shape, with uneven colony edges and AP 8/2 with 61.11% inhibition which had features of flat colony surface shape, white colour, with uneven edges of colonies.

POTENSI BERBAGAI JENIS RIZOBAKTERI SEBAGAI PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) DALAM MENINGKATKAN VIABILITAS DAN VIGOR BENIH KACANG TANAH Nadia Aulia Rizqi Jana; Nurhayati Nurhayati; Syamsuddin Syamsuddin
Jurnal Ilmiah Mahasiswa Pertanian Vol 8, No 1 (2023): Februari 2023
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak. Pemaanfaatan mikroorganisme rizobakteri atau dikenal sebagai plant growth promoting rhizobacteria (PGPR) untuk meningkatkan mutu benih melalui perlakuan benih yang diintegrasikan dengan mikroorganisme. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh penggunaan rizobakteri pemacu pertumbuhan tanaman pada perlakuan benih kacang tanah terhadap viabilitas dan vigornya. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Program Studi Agroteknologi Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh, mulai bulan januari hingga bulan maret 2022. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non faktorial. Terdapat 27 satuan percobaan yang meliputi 8 taraf rizobakteri dan 1 kontrol dengan 3 ulangan. Jenis rizobakteri II NA 4, II NA 13 dan III KB 3 berbeda nyata terhadap penggunaan rizobakteri jenis II NA 1, II NA 14, II KB 5, III KB 1, dan III SPA 1, namun berbeda tidak nyata terhadap perlakuan kontrol pada parameter potensi tumbuh maksimum (PTM), daya berkecambah (DB) dan keserempakan tumbuh (KST), hal ini dikarena setiap jenis rizobakteri memiliki peranan yang berbeda-beda, walaupun rizokbakteri yang digunakan pada penelitian ini tidak terlalu berperan sebagai pemacu pertumbuhan tanaman, akan tetapi rizobakteri tersebut masih berpotensi untuk digunakan sebagai bioprotektan.Potential of Various Genus / Species of Rhizobacteria as PGPR in Increasing the Viability and Vigor of Arachis hypogea LAbstract. Utilization of rhizobacterial microorganisms or known as plant growth promoting rhizobacteria (PGPR) to improve seed quality through seed treatment that is integrated with microorganisms. This study aims to determine the effect of seed treatment using p1ant growth promoting rhizobacteria on the viabi1ity and vigor of peanut seeds. This research was conducted at the Seed Science and Technology Laboratory of the Agrotechno1ogy Study Program, Facu1ty of Agricu1ture, Syiah Kua1a University, Darussa1am Banda Aceh, from January to March 2022. This study used a comp1etely randomized design (CRD) with a non-factorial pattern. The factors studied were 8 levels of rhizobacteria and 1 control with 3 rep1ications so that there were 27 experimenta1 units. The types of rhizobacteria II NA 4, II NA 13 and III KB 3 were significantly different from the use of rhizobacteria types II NA 1, II NA 14, II KB 5, III KB 1, and III SPA 1, but not significantly different from the control treatment in terms of maximum growth potential (PTM), germination capacity (DB) and growth simultaneity (KST), this is because each type of rhizobacteria has a different role, even though the rhizobacteria used in this study were not very acts as a plant growth promoter, but these rhizobacteria still have the potential to be used as bioprotectants.

Pengaruh Komposisi Media Tanam dan Dosis Pupuk NPK terhadap Pertumbuhan Bibit Kakao (Theobroma Cacao L.) Khairul Anwar; Trisda Kurniawan; Syamsuddin Syamsuddin
Jurnal Ilmiah Mahasiswa Pertanian Vol 7, No 3 (2022): Agustus 2022
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Abstrak . Penelitian ini bertujuan untuk mengetahui pengaruh komposisi media tanam dan dosis pupuk NPK yang tepat terhadap pertumbuhan bibit tanaman kakao serta untuk mengetahui interaksi antar kedua faktor tersebut. Penelitian ini telah dilaksanakan di Kampung Badak, Kecamatan Dabun Gelang, Kabupaten Gayo Lues dan Laboratorium Dasar Kampus USK PSDKU Gayo Lues. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 4x3 dengan 3 ulangan, sehingga terdapat 12 kombinasi perlakuan dan 36 unit percobaan. Ada 2 faktor yang diteliti yaitu pengaruh komposisi media tanam dan pupuk NPK. Faktor pertama adalah komposisi media tanam (M) terdiri dari 3 taraf : M 1 = Tanah + Arang Sekam + Limbah Kulit Kopi (2:1:1), M 2= Tanah + Arang Sekam+ Limbah Kulit Kopi (1:1:2), M 3 = Tanah + Arang Sekam + Limbah Kulit Kopi (1:2:1). Faktor kedua adalah pupuk NPK (P) yang terdiri dari 4 taraf : N 0 = 0 g/tanaman (kontrol), N 1 = 2 g/tanaman, N 2 = 4 g/tanaman, N 3= 6 gr/tanaman. Analisis data menggunakan ANOVA dan dilanjutkan dengan uji Beda Nyata Jujur (BNJ) pada taraf 0,05. Parameter yang diamati adalah tinggi tanaman, diameter pangkal batang, jumlah daun umur 30, 45, 60, 75 dan 90 HST, bobot basah berangkasan, panjang akar, volume akar dan bobot kering berangkasan. Hasil penelitian menunjukkan bahwa komposisi media tanam berpengaruh sangat nyata terhadap panjang akar 90 HST serta berpengaruh nyata terhadap jumlah helai daun 60 HST. pertumbuhan terbaik bibit kakao yang diamati dari berat kering berangkasan dijumpai pada komposisi media tanam tanah : arang sekam : limbah kulit kopi (1:2:1) Hasil penelitian menunjukkan bahwa perlakuan dosis pupuk NPK berpengaruh sangat nyata terhadap tanaman dan jumlah helai daun umur 30 , 45, 60, 75 dan 90 HST, diameter pangkal batang umur 45, 60, 75 dan 90 HST, berat basah berangkasan, panjang akar, volume akar dan berat kering berangkasan umur 90 HST serta berpengaruh nyata terhadap diameter pangkal batang umur 30 HST. pertumbuhan terbaik bibit kakao ditemukan pada dosis pupuk NPK 6 g/tanaman. Terdapat interaksi yang sangat nyata antara komposisi media tanam dan dosis pupuk NPK terhadap panjang umur 90 HST serta interaksi yang nyata terhadap jumlah helai daun umur 90 HST. Panjang akar umur 90 HST terbaik yang ditemukan pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (2:1:1) dengan dosis pupuk NPK 2 g/tanaman dan jumlah helai daun umur 90 HST terbaik ditemui pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (1:1:2) dengan dosis pupuk NPK 6 g/tanaman. volume akar dan berat kering berangkasan umur 90 HST serta berpengaruh nyata terhadap diameter pangkal batang umur 30 HST. pertumbuhan terbaik bibit kakao ditemukan pada dosis pupuk NPK 6 g/tanaman. Terdapat interaksi yang sangat nyata antara komposisi media tanam dan dosis pupuk NPK terhadap panjang umur 90 HST serta interaksi yang nyata terhadap jumlah helai daun umur 90 HST. Panjang akar umur 90 HST terbaik yang ditemukan pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (2:1:1) dengan dosis pupuk NPK 2 g/tanaman dan jumlah helai daun umur 90 HST terbaik ditemui pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (1:1:2) dengan dosis pupuk NPK 6 g/tanaman. volume akar dan berat kering berangkasan umur 90 HST serta berpengaruh nyata terhadap diameter pangkal batang umur 30 HST. pertumbuhan terbaik bibit kakao ditemukan pada dosis pupuk NPK 6 g/tanaman. Terdapat interaksi yang sangat nyata antara komposisi media tanam dan dosis pupuk NPK terhadap panjang umur 90 HST serta interaksi yang nyata terhadap jumlah helai daun umur 90 HST. Panjang akar umur 90 HST terbaik yang ditemukan pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (2:1:1) dengan dosis pupuk NPK 2 g/tanaman dan jumlah helai daun umur 90 HST terbaik ditemui pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (1:1:2) dengan dosis pupuk NPK 6 g/tanaman. Terdapat interaksi yang sangat nyata antara komposisi media tanam dan dosis pupuk NPK terhadap panjang umur 90 HST serta interaksi yang nyata terhadap jumlah helai daun umur 90 HST. Panjang akar umur 90 HST terbaik yang ditemukan pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (2:1:1) dengan dosis pupuk NPK 2 g/tanaman dan jumlah helai daun umur 90 HST terbaik ditemui pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (1:1:2) dengan dosis pupuk NPK 6 g/tanaman. Terdapat interaksi yang sangat nyata antara komposisi media tanam dan dosis pupuk NPK terhadap panjang umur 90 HST serta interaksi yang nyata terhadap jumlah helai daun umur 90 HST. Panjang akar umur 90 HST terbaik yang ditemukan pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (2:1:1) dengan dosis pupuk NPK 2 g/tanaman dan jumlah helai daun umur 90 HST terbaik ditemui pada kombinasi media tanam tanah : arang sekam : limbah kulit kopi (1:1:2) dengan dosis pupuk NPK 6 g/tanaman. Effect of Planting Media Composition and NPK Fertilizer Dosage on Cocoa Seed Growth (Theobroma cacao L.)Abstract. This study aims to determine the effect of the composition of the planting medium and the right dose of NPK fertilizer on the growth of cocoa plant seeds and to determine the interaction between these two factors. This research was carried out in Badak Village, Dabun Bracelet District, Gayo Lues Regency and the Basic Laboratory of the USK PSDKU Gayo Lues Campus. This study used a 4x3 factorial randomized block design (RAK) with 3 replications, so there were 12 treatment combinations and 36 experimental units. There are 2 factors studied, namely the influence of the composition of the planting media and NPK fertilizer. The first factor is the influence of the composition of the planting medium (M) consisting of 3 levels: M1= Soil + Husk Charcoal + Coffee Peel Waste (2:1:1), M2=Soil + Husk Charcoal + Coffee Peel Waste (1:1:2 ), M3 = Soil + Husk Charcoal + Coffee Peel Waste (1:2:1). The second factor was NPK fertilizer (P) which consisted of 4 levels: N0 = 0 g/plant (control), N1 = 2 g/plant, N2 = 4 g/plant, N3 = 6 g/plant. Data analysis used ANOVA and continued with the Honest Significant Difference (BNJ) at 0.05 level. Parameters observed were plant height, stem diameter, number of leaves at 30, 45, 60, 75 and 90 DAP, dry weight of stem, root length, volume of root and dry weight of root. The results showed that the composition of the growing media had a very significant effect on root length at 90 DAP and significantly affected the number of leaves at 60 DAP. The best growth of cacao seedlings observed from dry weight was found in the composition of the growing media: soil: husk charcoal: coffee husk waste (1:2:1) The results showed that the dose of NPK fertilizer had a very significant effect on plant height and number of leaves at 30, 45, 60, 75 and 90 DAP, stem diameter at 45, 60, 75 and 90 DAP, dry weight, root length, root volume and dry weight at 90 DAP and significantly affected the diameter of the stem at 30 DAP. The best growth of cocoa seedlings was found at a dose of 6 g/plant of NPK fertilizer. There was a very significant interaction between the composition of the growing media and the dose of NPK fertilizer on root length at 90 DAP and a significant interaction with the number of leaves at 90 DAP. The best root length at 90 DAP was found in the combination of soil: husk charcoal: coffee husk waste (2:1:1) with a dose of NPK fertilizer of 2 g/plant and the best number of leaves at 90 DAP was found in the combination of soil: charcoal. husk : coffee husk waste (1:1:2) with a dose of NPK fertilizer 6 g/plant.

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