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KARAKTERISASI RIZOBAKTERI SEBAGAI AGENS BIOKONTROL SERTA UJI IN VITRO TERHADAP PATOGEN Fusarium oxysporum f.sp.lycopersici (Sacc.) TANAMAN TOMAT (Solanum lycopersicum) DAN PERANNYA SEBAGAI PEMACU PERTUMBUHAN TANAMAN Risya Putri Annura; Syamsuddin Syamsuddin; Halimursyadah Halimursyadah
Jurnal Agrista Vol 25, No 2 (2021): Volume 25 Nomor 2 Agustus 2021
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Tujuan pada penelitian ini adalah untuk mengetahui peran rizobakteri sebagai agens biokontrol terhadap patogen F.oxysporum tanaman tomat secara in vitro dan perannya sebagai pemacu pertumbuhan tanaman. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh yang berlangsung pada Desember sampai Maret 2021. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial yang terdiri dari 13 perlakuan rizobakteri dan diulang sebanyak 3 kali. Hasil penelitian menunjukkan bahwa pengujian rizobakteri sebagai agens biokontrol berpengaruh sangat nyata terhadap daya hambat dan laju penghambatan pertumbuhan koloni patogen F. oxysporum dengan daya hambat tertinggi dijumpai pada isolat LDG 5/2 dengan penghambatan 64.13% yang digolongkan ke dalam aktivitas tinggi (+++=61-75% DH). Laju penghambatan pertumbuhan patogen tertinggi terdapat pada isolat LDG 6/4C yaitu 2.77 mm.hari-1. Isolat dengan kemampuan memproduksi HCN dijumpai pada isolat LDG 4/1P dengan jumlah produksi HCN tinggi (+++HCN). Peran rizobakteri sebagai pemacu pertumbuhan tanaman dilihat dari karakterisasinya yang mampu memproduksi IAA dan melarufkan fosfat. Isolat LDG 3/3 mampu memproduksi IAA tertinggi yaitu 1.270  µg.ml-1 filtrat dan isolat rizobakteri yang mampu melarutkan fosfat dengan nilai indeks pelarutan fosfat tertinggi yaitu isolat LDG 4/1P yaitu 51.14%. Characterization of Rhizobacteria as a Agents Biocontrol and In Vitro Test of Pathogen Fusarium oxysporum f.sp. lycopersici (Sacc.) Tomato Plants (Solanum lycopersicum) and its Role as Plant Growth Promoting This study aims to determine the role of rhizobacteria as a biocontrol agent against the pathogen F.oxysporum tomato plant in vitro and its role as a plant growth promoting rhizobakteria (PGPR). This research was conducted at the Laboratory of Seed Science and Technology, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh from December to March 2021. The study used a Completely Randomized Design (CRD) non factorial pattern that consisting of 13 rhizobacteria treatments and repeated 3 times. The results showed that testing rhizobacteria as a biocontrol agent had a very significant effect on the inhibition and rate of inhibition of the growth of the pathogenic colony F. oxysporum with the highest power of inhibition in LDG 5/2 isolates with 64.13%  which was classified as high activity (+++ = 61-75% IP). The highest inhibition rate of pathogenic growth was found in LDG 6/4C isolates, namely 2.77 mm.day-1. Isolates with the ability to produce HCN was found in LDG 4/1P with high amounts of HCN production (+++ HCN).  The role of rhizobacteria as plant growth promoting is seen from its characterization which is able to produce IAA and dissolve phosphate. LDG 3/3 isolates were able to produce the highest IAA, namely 1,270 µg.ml-1 filtrate and rhizobacterial isolates which were able to dissolve phosphate with the highest phosphate dissolving index value, namely LDG 4/1P isolates of 51.14%.
Respon Pertumbuhan dan Produksi Plasma Nutfah Padi Lokal Aceh Terhadap Sistem Budidaya Aerob Efendi Efendi; Halimursyadah Halimursyadah; Hotna Riris Simajuntak
Jurnal Agrista Vol 16, No 3 (2012): Volume 16 Nomor 3 Desember 2012
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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ABSTRAK. Penelitian bertujuan untuk mengetahui respon pertumbuhan dan produksi 30 varietas padi lokal Aceh terhadap sistem budidaya padi aerob dengan menggunakan pendekatan secara intermitten selama fase pertumbuhan vegetatif. Respon pertumbuhan dan produksi tanaman padi dipelajari dengan mengamati karakter morfo-agronomis. Hasil penelitian menunjukkan bahwa varietas sangat mempengaruhi tinggi tanaman. Tinggi tanaman padi varietas lokal ternyata sangat bervariasi antar satu varietas dengan varietas lainnya. Varietas Pade Pangku, Sikuneng, Rom Mokot, Sepuluo, Pade Mas, dan Sigupai merupakan kelompok varietas yang paling tinggi. Hasil penelitian menunjukkan juga bahwa varietas sangat mempengaruhi jumlah anakan varietas padi lokal Aceh. Varietas Pandrah, Asi Puteh, Sigudang, Bo Santeut, Pade Mirah, Pade P66,  Sanbei, Aweuh, Acong, Bo 100, Salah Mayang Ru, dan Sipirok merupakan varietas-varietas yang memiliki jumlah anakan yang tinggi. Sedangkan tanaman yang paling cepat berbunga terdapat pada varietas Pade P66, Sigudang, dan Pade Das. Sementara varietas yang paling lama mengeluarkan bunga terdapat pada varietas Rangkop Mirah. Varietas berpengaruh sangat nyata terhadap hasil tanaman per pot. Perbedaan varietas menghasilkan berat gabah per pot yang sangat bervariasi. Hasil tanaman per pot terberat dijumpai pada varietas Pade Manggeng, Aweuh, Sigupai, Bo Santeut, Sikuneng, dan Pade Mas. Respon pertumbuhan dan produksi tanaman padi varietas lokal Aceh lebih baik dibandingkan padi varietas Nasional apabila dibudidayakan dengan sistem padi aerob.The Response of Local Acehnese Germplasm of Rice to Growth and Production in Aerobic ConditionABSTRACT. The purpose of this research is to study responds of 30 variety of Acehnese local rice to aerobic cultivation method with intermitten approach during vegetative phase of rice plant. Morpho-agronomic characters were observed to evaluate the responds of the varieties. Results of study showed that variety was significantly affected plant height, and indicated wide variation among varieties for the plant height. Pade Pangku, Sikuneng, Rom Mokot, Sepuluo, Pade Mas, and Sigupai were the varieties that have taller plant height than others. The factor of variety also significantly affected number of tillers. Variety of Pandrah, Asi Puteh, Sigudang, Bo Santeut, Pade Mirah, Pade P66,  Sanbei, Aweuh, Acong, Bo 100, Salah Mayang Ru, and Sipirok were the varieties that have bigger number of tillers. Some varieties, PadeP66, Sigudang, and Pade Das produced flower earlier than others. Rangkop Mirah variety was the longest time to initiate it flower. We also found that variety significantly affected yield of rice per pot. Pade Manggeng, Aweuh, Sigupai, Bo Santeut, Sikuneng, and Pade Mas were the varieties that have higher yield of rice per pot compared than others. When the varieties cultivated in aerobic condition, the responds of Acehnese local variety was better than national superior variety.
Pengaruh Konsentrasi Pupuk Organik Cair Super Bionik dan Varietas Kacang Tanah terhadap Pertumbuhan dan Hasil Zuyasna Zuyasna; Halimursyadah Halimursyadah; Chandra Saputra
Jurnal Agrista Vol 14, No 3 (2010): Volume 14 Nomor 3 Desember 2010
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Effect of Organic Liquid Fertilizer and Peanut Variety on the Growth and YieldABSTRACT. The objective of this study were to investigate several varieties and liquid organic fertilizer on the growth at the yield of peanut of the tsunami affected area and also to identify the interaction between the two factors. The research was conducted at the Lamnyong river flood plain which is located at west sector of Komplek Pelajar Mahasiswa (KOPELMA) Darussalam – Banda Aceh from March to Mei 2009. Randomized completely block design with two factor was used on this experiment using three replications. The first factor was peanut variety (i.e. Singa, Kuala Batee and Tangse), and the second factors was Super Bionik fertilizer concentrations (i.e. 0 cc; 0.9 cc; 1,8 cc /225cc). The variety significantly influenced the plants height on 45 days after planting, the amount of ginofore, the plant wet weight, and 100 seed weight, however the variety not significantly influenced other parameters (i.e. the amount of the branches at 45 days after planting, the total pods, and the amount of full pod). The result showed that Super Bionic concentrations not significantly influenced all parameters observed, and no interactions between the variety and the fertilizer concentration occurred on the parameters observed.
UJI KEMAMPUAN ISOLAT RIZOBAKTERI INDIGENOUS DARI BEBERAPA SUMBER LOKASI SEBAGAI PLANT GROWTH PROMOTING RHIZOBACTERIA (PGPR) TERHADAP PERTUMBUHAN TIGA VARIETAS TANAMAN NILAM ACEH Dian Novira Rizva; Nurhayati Nurhayati; Halimursyadah Halimursyadah
Jurnal Agrista Vol 25, No 3 (2021): Volume 25 Nomor 3 Desember 2021
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Rizobakteri adalah kelompok bakteri yang hidup berkoloni di area perakaran berperan mendukung pertumbuhan dan perkembangan tanaman. Penelitian ini bertujuan untuk mengetahui interaksi antara isolat rizobakteri indigenous dan varietas terhadap pertumbuhan tanaman nilam Aceh. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi Fakultas Pertanian Universitas Syiah Kuala, Darussalam Banda Aceh dan Kebun Nino Park, Sektor Timur mulai Oktober 2020 sampai Februari 2021. Percobaan ini menggunakan rancangan acak kelompok (RAK) pola faktorial 10x3 dengan 3 ulangan. Ada 2 faktor yang diteliti, faktor pertama adalah jenis isolat rizobakteri (R) yang terdiri dari 10 taraf (PS 4/2, PS 5/6C, PS 6/3A, PS 7/1, 8/4K, PS 8/8 PK, KI 8/1, KI 8/3, CL 4/1), dan Faktor kedua adalah jenis varietas (V) yang terdiri dari 3 taraf (Tapaktuan, Lhokseumawe, Sidikalang). Hasil penelitian menunjukkan terdapat interaksi yang sangat nyata antara faktor isolat rizobakteri dan varietas.  Kombinasi terbaik terdapat pada isolat rizobakteri PS 4/2 dan varietas Tapak Tuan pada peubah jumlah daun dan cabang umur 8 dan 12 MSA dan isolat rizobakteri KI 8/3 dan varietas Sidikalang pada peubah jumlah cabang 8 dan 12 MSA. Kombinasi isolat rizobakteri PS 6/3A dan varietas Sidikalang dan varietas Lhokseumawe berturut-turut pada peubah jumlah daun 8 dan 12 MSA, serta kombinasi isolat rizobakteri KI 8/1 dan varietas Lhokseumawe pada peubah jumlah daun 8 MSA. Ability Testing of Indigenous Rhizobacteria Isolates from Several Sources of Location as Plant Growth Promoting Rhizobacteria (PGPR) on The Growth of Three Variety of Aceh Patchouli Plants Rhizobacteria are a group of bacteria that live in colonies in the root area and play a role in supporting plant growth and development. This study aims to determine the interaction between indigenous rhizobacteria isolates and varieties on the growth of Aceh patchouli. This research was carried out at the Seed Science and Technology Laboratory, Department of Agrotechnology, Faculty of Agriculture, Universitas Syiah Kuala , Darussalam Banda Aceh and Nino Park Gardens, Sektor Timur from Oktober 2020 to February 2021. This experiment used a 10x3 factorial randomized block design (RAK) with 3 replications. There are 2 factors studied, the first factor is the type of rhizobacteria isolate (R) which consists of 10 levels (PS 4/2, PS 5/6C, PS 6/3A, PS 7/1, 8/4K, PS 8/8 PK, KI 8/1, KI 8/3, CL 4/1) and the second factor is the type of variety (V) which consists of 3 levels (Tapaktuan, Lhokseumawe, Sidikalang). The results showed that there was a very significant interaction between rhizobacteria isolates and varieties. The best combination was found in PS 4/2 rhizobacteria isolates and Tapak Tuan varieties on the variable number of leaves and branches aged 8 and 12 MSA and rhizobacteria isolates KI 8/3 and Sidikalang varieties on the variables number of branches 8 and 12 MSA. The combination of rhizobacteria isolates PS 6/3A with Sidikalang and Lhokseumawe varieties on leaf number variables 8 and 12 MSA, respectively, and the combination of rhizobacteria isolates KI 8/1 and Lhokseumawe varieties on leaf number variables 8 MSA
Pertumbuhan dan Hasil Tanaman Tumpangsari Tomat (Lycopersicum esculentum Mill.) dan Bawang Merah (Allium esculentum L.) melalui Kobinasi Jarak Tanam dan Konsentrasi Pupuk Hayati Cut Nur Ichsan; Halimursyadah Halimursyadah; Elisa Susanti
Jurnal Agrista Vol 15, No 2 (2011): Volume 15 Nomor 2 Agustus 2011
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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ABSTRACT. Tujuan penelitian ini adalah untuk menentukan efektivitas jarak tanam dan konsentrasi pupuk hayati terhadap pertumbuhan dan hasil tanaman tumpang sari tomat dan bawang merah. Hasil penelitian menunjukkan bahwa jarak tanam berpengaruh terhadap tinggi tanaman pada 10 dan 55 HST, jumlah buah dan bobot total tanaman tomat. Terdapat interaksi antara jarak tanam dan konsentrasi pupuk hayati terdapat tinggi tanaman pada 55 HST, rata-rata bobot buah per tanaman tomat. Tanaman tomat tertinggi pada 55 HST dijumpai pada perlakuan jarak tanam 60cmx60cm tanpa pemberian pupuk hayati memberikan dan jarak tanam 70cmx60cm dengan pemberian pupuk hayati sebanyak 2% memberikan rata-rata bobot buah tomat tertinggi.Growth and Yield of Intercropping Tomato (Lycopersicum esculentum Mill.) and Onion (Allium esculentum L.) due to Different Spacing and Concentration of BiofertilizerABSTRACT. The objective of study were to determine the effective row-spacing and concentration of biofertilizer on growth and yield of intercropping tomato and onion. The result showed that the row spacing have significant effect on height of tomato tree at 10 and 55 DAP, amount of fruit and total weight of per tomato plant. Their were significant interaction between row spacing and concentration of biofertilizer on height at 55 DAP and average weight of fruit per plant of tomato. The row spacing 60 cm x 60 cm and 0% of biofertilizer gave the highest height of tomato tree at 55DAP and row spacing 70 cm x 60 cm with 2% biofertilizer gave heaviest average of weight of tomato fruit per plant.
Pengaruh Laju Pengeringan Terhadap Perubahan Fisiologi BENIH Avicennia marina (Forsk.) Vierh. Halimursyadah Halimursyadah
Jurnal Agrista Vol 12, No 1 (2008): Volume 12 Nomor 1 April 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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The Effect of Drying Rate on Physiological Changes in Seed of Avicennia marina (Forsk.) Vierh.ABSTRACT. Avicennia marina seed has been classified a highly recalcitrant that need the right handling for maintaining seed viability. The objective og this study was to investigate physiological, biochemical, anatomical changes at various drying rate, fast, medium and slow drying. Seed moisture content, germination percentage, speed of germination, index of vigor, time to reach 50% germination, electrolyte leachates, oligosaccharide content were determined, and anatomical of embryonic axes was observed at various times durng desiccation. The result of the experiment show that the moisture content of A. marina seed was 60.75-64.67% at shedding. Time to reach the lowest moisture content (32-33%) at fast, medium and slow drying were 6, 10 and 14 days, respectively. First and final count in germination test were 13 and 22 days. Critical moisture content of seed based on time to reach 50% viability (P50) were 37.25, 43.28, and 45.92% for fast, medium and slow drying, respectively. The critical moisture content based on speed of germination were 36.51, 42.47, and 45.01% for fast, medium and slow drying, respectively.
Penggunaan organic priming dan periode inkubasi untuk invigorasi benih cabai merah (Capsicum annum L.) kadaluarsa pada stadia perkecambahan Halimursyadah Halimursyadah; Jumini Jumini; Muthiah Muthiah
Jurnal Floratek Vol 10, No 2 (2015): Oktober 2015
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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Using Organic Priming and Incubation Period for Invigoration of Chilli Seed Expired at Germination Stage ABSTRACT. The purpose of this study was to determine the effect of organic priming and incubation period to increase viability and vigor of expired chilli seeds and interaction between of two factors. This research was conducted in the Laboratory of Seed Science and Technology Faculty of Agriculture, Syiah Kuala University in Banda Aceh, from March until November 2012. Using completely randomized design (CRD) factorial, the first factor is organic priming (banana extract and coconut water) and the second factor is incubation period (24, 48, 72 hours). The results showed that interaction between types of organic priming and incubation period significan affect to increase viability and vigor of expired chilli seeds, especially on potential growth, germination, speed of germination, first count germination and T50. The best treatment showed by using coconut water and 48 hours incubation periods.
PERUBAHAN FISIOLOGI DAN KANDUNGAN KLOROFIL SELAMA PEMASAKAN SERTA HUBUNGANNYA DENGAN VIABILITAS BENIH JARAK PAGAR (Jatropha curcas L.) Hasanuddin Hasanuddin; Halimursyadah Halimursyadah; Trisda Kurniawan
Jurnal Floratek Vol 7, No 2 (2012)
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala

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The objectives of this research were to study physiological and chlorophyll changes during seed ripening and find quick parameters as indicators to determine seed physiological maturity level. A randomized completely block design with 5 treatments and 3 replications was used in this research. The treatments were (1) dark green fruit, 42 days after anthesis (DAA), (2) yellowish green fruit, 47 DAA, (3) fully green fruit, 52 DAA, (4) brownish yellow fruit, 57 DAA, and (5) dark brown fruit, 62 DAA. The results showed that jathropa seed was physiologically mature at 57 DAA with criteria: fruit color was brownish yellow, fruit skin was soft and easily hand-opened, and seed was black. These were also supported by seed dry weight, germination percentage, germination rate and first count germination. There were negative relationships between seed chlorophyll content and germination percentage, germination rate and first count germination. 
PENGARUH KONDISI SIMPAN TERHADAP VIABILITAS DAN VIGOR BENIH Avicennia marina (Forsk.)Vierh. PADA BEBERAPA PERIODE SIMPAN Halimursyadah Halimursyadah
JURNAL AGROTROPIKA Vol 17, No 2 (2012): Agrotropika Vol.17 No.2 2012
Publisher : Universitas Lampung

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The objective of the research was to determine the optimum storage condition (temperature and relative humidity) during 10 weeks with interval 2 weeks to maintain the seed viability and vigor. The variables were seed moisture content, gemination percentage, speed of germination, index of vigor, time to reach 50% germination, and electrolyte leachates. The research used split plot experi-mental design, with storage condition (AC and room temperature) as the main plot and storage period (0, 2, 4, 6, 8, and 10 weeks) as the subplot. The results showed seed stored at 90% relative humidity (using saturated KCl) and 19-200C temperature for 2 weeks storage periods significantly decrease in germination percentage (66.67%), speed of germination (64.56%), index of vigor (40.00%) and increased in time to reach 50% ger-mination (12.25 days) compared to the control, with germination percentage (94.67%), speed of germination (91.97%), index of vigor (49.33%), and time to reach 50% germination (11.25 days). Seed stored at 90% rela-tive humidity (using saturated KCl) and 27-280C temperature for 2 weeks storage periods showed significant decrease in germination percentage (36.00%), speed of germination (33.21%), index of vigor (21.33%), and increase in time to reach 50% germination (13.41 days) and electrolyte leachates (42.99 μScm-1g-1) as com-pared to untreated with 89.33% germination, 87.98% speed of germination, 49.33% index vigor, time to reach 50% germination (11.75 days), and electrolyte leachates (36.16 μScm-1g-1). Storage condition using saturated NaCl at 19-200C (AC) or 27-280C (ambient) with 80% RH and 75% RH, respectively, showed decrease in ger-mination compared to using saturated KCl at the same storage temperature. At 4 and 6 weeks storage periods at AC or ambient temperature showed declining in viability and vigor. Total loss of viability and vigor occured at 8 and 10 weeks storage. The seeds can be stored at 90% RH (using saturated KCl) and low temperature (AC) for up to 2 weeks; after that period, viability and vigor declined. Key words: storage condition, strorage period, seed viability, and vigor
PENGARUH MEDIA TANAM DAN LAMA PERENDAMAN DENGAN AUKSIN TERHADAP PERTUMBUHAN STEK BASAL DAUN NANAS (Ananas Comosus L. Merr.) Chairunnisak Chairunnisak; Hasanuddin Hasanuddin; Halimursyadah Halimursyadah
Prosiding Seminar Nasional Biotik Vol 3, No 1 (2015): PROSIDING SEMINAR NASIONAL BIOTIK III 2015
Publisher : Prosiding Seminar Nasional Biotik

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Penelitian ini bertujuan untuk mengetahui media tanam yang terbaik dan lama perendaman dengan auksin yang tepat serta interaksi antara kedua faktor tersebut terhadap pertumbuhan stek basal daun nanas. Penelitian ini dilaksanakan di Stasiun Penelitian Organik Fakultas Pertanian Universitas Syiah Kuala Darussalam Banda Aceh, yang dimulai pada tanggal 16 April sampai 16 Juli, 2014. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) pola faktorial 4 x 4 dengan 3 ulangan. Ada dua faktor yang diteliti yaitu faktor media tanam (tanah, arang sekam, cocopeat, tandan kelapa sawit) dan lama perendaman dengan auksin (1 menit, 3 menit, 5 menit, 7 menit). Parameter yang diamati meliputi persentase stek bertunas umur 14, 28, 42 dan 56 HST, tinggi tunas dan jumlah daun umur 14, 28, 42, 56, 70 dan 84 HST, persentase stek berakar umur 56 HST, jumlah akar dan panjang akar umur 84 HST. Terdapat interaksi yang sangat nyata antara media tanam dan lama perendaman dengan auksin terhadap persentase stek bertunas umur 14, 28, 42, dan 56 HST, tinggi tunas umur 14, 28, 42, 56, 70 dan 84 HST, jumlah daun umur 14, 28, 42, 70 dan 84 HST, persentase stek berakar umur 56 HST, panjang akar umur 84 HST, berpengaruh nyata terhadap jumlah daun umur 56 HST dan tidak berpengaruh nyata terhadap jumlah akar umur 84 HST. Kombinasi yang cenderung baik dijumpai pada media tanam tandan kelapa sawit dengan lama perendaman 7 menit.