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Khusnan k
Akademi Peternakan Brahmaputra, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

 1 
Uji pigmen dan deteksi kapsul polisakarida pada Staphylococcus aureus isolat asal broiler PIGMENT TEST AND DETECTION OF POLISAKARIDA CAPSULES ON Staphylococcus aureus ISOLAT BROILER Khusnan Khusnan; Dwi Kusmanto
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.109 KB) | DOI: 10.19087/jveteriner.2019.20.3.369

Abstract

Staphylococcus aureus is a pathogenic bacterium causing disease in humans and animals. In broilers it cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections and arthritis and bumblefoot. The ability of Staphylococcus aureus to cause disease depends on the virulence factors they bear. The purpose of this research is to investigate the distribution of pigment production type and the existence of genes of polysaccharide capsule phenotype and genotype as determinant factor of virulence of bacteria on 15 isolates of Staphylococcus aureus from broiler. Pigment production test showed that 86.7% of isolates producing yellow pigment and 13.3% isolates produce orange pigment. The detection of polysaccharide capsules was phenotypically performed with hydrophobicity test with serum soft agar medium (SSA) showed 53.3% isolate grow compact and 46,7% isolate grown difuse and hydrophobicity test by salt aggregation test method (SAT) showed 66,7% hydrophil and 33.3% are hydrophobic. Genotype detection of polysaccharide capsule genes by polymerase chain reaction (PCR) showed 66.7% detected cap5 (amplicon 361bp) and 33.3% detected cap8 (173bp ampliole). The type of pigment production and the presence of polysaccharide capsules are some of the virulent factors in Staphylococcus aureus.
Staphylococcus aureus Penghasil Pigmen Kuning yang Diisolasi dari Kejadian Bumblefoot pada Broiler Lebih Patogen Dibanding Penghasil Pigmen Putih (STAPHYLOCOCCUS AUREUS PRODUCING YELLOW PIGMENT ISOLATED FROM BUMBLEFOOT CASE IN BROILER CHICKENS IS MORE PAT Khusnan .; Wahyu Prihtiyantoro; Mitra Slipranata
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.768 KB)

Abstract

A study to evaluate the correlation between the pigments produced by Staphylococcus aureusisolateswith their adhesion capability on epithelial cells and phagocytes activity of macrophages in vitro.Ten S.aureus isolatesfrom bumble foot and arthritis casesin broiler were used in this study. The adhesion assaywas performed using epithelial cell derived from human buccal epithelial cells whereas phagocytic activitywas conducted using mouse peritoneal macrophage cells.The results showed that S. aureus isolates frombroiler produced both yellow and white pigments. Staphylococcus aureus producing white pigment adheredto human buccal epithelial cells with the density of about 24,25 bacteria/cell, which were higher than thoseof Staphylococcus aureus producing yellow pigmentabout 23,78 bacteria/cell. Staphylococcus aureusproducing white pigment was more phagocytosed by macrophages than S. aureusproducing yellow pigment,with an average phagocytic activiry of19,86 bacteria/cellas compared to15,96of bacteria/cell, respectively.
Distribusi Gen Enterotoksin Staphylococcus aureus dari Susu Segar dan Pangan Asal Hewan Siti Isrina Oktavia Salasia; Khusnan -; Sugiyono -
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is a potential pathogen causing disease in human and animals due to severalvirulence factors. Staphylococcal enterotoxins which is responsible for foodborne disease is considered tobe one of the important virulence factor for the bacteria. The research was conducted to identify variousenterotoxin genes of S. aureus. Twenty three S. aureus isolates from milk cows (12 isolates) and foodanimal products (11 isolates) were used to study various enterotoxins genes (sea, seb, sec, sed, see, seg, seh,sei, and sej). The enterotoxins genes of S. aureus were investigated by using polymerase chain reaction(PCR) with specific primers. There were 3 isolates (13.04%) negative for staphylococcal enterotoxin genes.Twenty isolates (86.96%) harboured for one or more staphylococcal enterotoxin genes such as: sec (6 isolates/26.09%), see, seh for 1 isolate (4.35%), combination of 2 genes se (b,i), se (c,g), se (g,i) for 1 isolate of each(4.35%), se(c,e) for 2 isolates (8.70%), se(b,c) for 4 isolates (17.39%). Staphylococcal enterotoxin could bedetected in 3 combination genes of se(b,c,i), se(c,e,i), se(c,g,i) for 1 isolate of each (4.35%).
ISOLATION AND PHENOTIPICAL CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM SLAUGHTERED HOUSE WASTE AND CARCASS OF CHICKEN Khusnan -; Siti Isrina Oktavia Salasia; Sugiyono -
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Staphylococcus aureus is known as a potential agent that cause food poisoning. Chicken meats could be as vehicles of food poisoning cases while they contaminated by agents during slaughtered and meat processing as well as infected chickens by S. aureus itself. The aims of the research were to isolated and characterized of S. aureus from liquid wastes of chicken slaughtered house and washing water of chicken carcass. The characterization of S aureus based on mannitol salt agar (MSA), catalase and coagulation assay, production of hemolysins, hydrophobicity test, and the reaction of hemaglutination. Thirty seven samples used in this study were collected from 16 liquid wastes slaughtered chicken house and 21 samples from washing water of chicken carcasses.The research resulted 18 S. aureus isolated from liquid wastes slaughtered house (4 isolates) and from wahing water of chicken carcasses (14 isolates). All 18 isolates fermented mannitol, catalase and coagulase positive, contain of hydrophilic surfaces. Among 18 S. aureus isolates could agglutinate of rabbit erythrocytes for 55%, produced ?-hemolysis (12%) and ?-hemolysis (88%).
Deteksi Faktor Virulensi Secara Fenotip pada Escherichia coli Isolat Susu Mentah Sapi Perah Khusnan Khusnan; Agus Purnomo
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.451 KB) | DOI: 10.19087/jveteriner.2022.23.1.42

Abstract

Susu sapi merupakan media pertumbuhan bakteri patogen maupun non patogen. Escherichia coli mrupakan salah satu bakteri yang sering ditemukan pada susu sapi mentah. Escherichia coli patogen pada sapi dapat menyebabkan mastitis maupun radang usus serta dapat menyebabkan masalah kesehatan pada manusia seperti diare, kolitis hemoragik dan sindrom uraemik hemolitik. Hemaglutinin dan hemolisin merupakan faktor virulen yang penting pada E. coli patogen, karena berperan sebagai faktor adhesin, dan faktor pertahanan terhadap fagositosis. Tujuan penelitian ini mendeteksi hemaglutinin dan hemolisin E. coli isolat asal susu sapi mentah serta kemampuan adhesi pada sel epitel serta pertahanan terhadap fagosit netrofil. Hasil penelitian ini menunjukkan 100% isolat E. coli tidak memiliki hemaglutinin dan 17,2% merupakan E. coli hemolitik. Pada uji adhesi mampu melekat pada sel epitel bukalis 46,87 bakteri/sel. Pada uji fagositosis isolat-isolat hemolitik lebih sedikit difagosit oleh neutrofil dibandingkan isolat non hemolitik (2,64 dibanding 3,1 bakteri) tiap neutrofil (P<0,05). Ditemukannya E. coli patogen pada susu mentah menegaskan pentingnya pasteurisasi pada susu sebelum dikonsumsi.
Co-Authors Agus Purnomo Aris Purwantoro Dwi Kusmanto Dwi Kusmanto Fatkhanudin Aziz Hartatik Hartatik Hartatik Hartatik Imron Rosyidi Khusnan Khusnan Mitra Slipranata Mitra Slipranata Mitra Slipranata Mitra Slipranata Siti Isrina Oktavia S. Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Sugiyono - Wahyu Prihtiyantoro Wahyu Prihtiyantoro