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All Journal Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
Al Azhar
Laboratorium Biokimia Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Medika Veterinaria

 1 
12. Isolate Of Bacteriua Cellulase Enzyme Production In The Rumen Of Aceh Cattle Base On Analysis Homology 16S rRNA Indah Melzana; Safika Safika; Darmawi Darmawi; Erina Erina; Fakhrurrazi Fakhrurrazi; Al Azhar
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.3415

Abstract

The rumen of cattle is well known one of a bacterium cellulolitic production that have ability to degrade cellulose for produce cellulase enzymes. The aims of the present study is to determine the type of bacteria produced cellulase enzymes in the rumen of cattle aceh. The diversity of cellulolitic bacteria is determined by  analysis of 16S rRNA genes. Isolation of cellulolytic bacteria, total DNA isolation, agarose gel electrophoresis, amplification of the 16S rRNA gene, determining the DNA sequence, DNA sequence homology and alignment of DNA sequences. Isolation of bacterium cellulolitic, isolating total DNA, gel electroforesis agarosa, amplified of gen 16S rRNA, determination the DNA barcode,  homology DNA barcode, and alignment of DNA sequences. The sample of rumen liquid was taked from RPH , then maked isolation of total DNA to get pure DNA from bacterium whos result and amplification DNA of bacterium cellulolitic separated by electroforesis gel agarosa. Gene of bacterium cellulolitic was isolated based Polymerase Chain Reaction methode. Alignment is done by using BlastN program, then use CLustalW program.  It can be concluded that the species of bacteria (FKH_USK_6) in rumen has a closeness with Coronabacter sakazakii bacteria strain PNP8 of 97% according to the sizeof the DNA sequence and the same position on an existing gene in GenBank.
KADAR GLUKOSA DARAH ANJING KAMPUNG (Canis familiaris) SEBELUM DAN SESUDAH BERBURU DI KENAGARIAN MUNGO KECAMATAN LUAK KABUPATEN LIMA PULUH KOTA PROVINSI SUMATERA BARAT (Blood Glucose Levels of Dogs (Canis familiaris) Pre and Post Hunting in Kenagarian Mungo, Luak, Lima Puluh Kota, and West Sumatera) Alkhalidri Murfi; Zuhrawati Zuhrawati; Sugito Sugito; Rusli Rusli; Al Azhar; Abdul Harris
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v10i1.4037

Abstract

The objective of this study was to examine glucose level in dog's blood before and after hunting activity. This research used 25 local dogs which is kept by Persatuan Olah Raga Buru Babi (PORBI) located at Kenagarian Mungo Sub-district Luak District Kabupaten Lima Puluh Kota West Sumatera. Data were analyzed with T-test. Results showed that average of dog blood glucose level before hunting was 91.56±7.70 mg/dl and significantly decrease (P0.05) to 57.84±5.04 mg/dL after hunting activity. As a conclusion, blood glucose level of dog before hunting activity was found in normal range but lowered glucose level were observed after hunting activity.
Co-Authors Abdul Harris Afira Rafina Alkhalidri Murfi Darmawi Darmawi Eliawardani Eliawardani Erina Erina Fakhrurrazi Fakhrurrazi Indah Melzana Nuzul Asmilia Rusli Rusli Safika S, Safika Sugito Sugito Yudha Fahrimal Zuhrawati Zuhrawati