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AKTIVITAS ANTELMINTIK BIJI Veitchia merrillii TERHADAP Ascaridia galli SECARA IN VITRO Abdullah Hamzah; Muhammad Hambal; Ummu Balqis; Darmawi Darmawi; Maryam Maryam; Rosmaidar Rosmaidar; Farida Athaillah; Muttaqien Muttaqien; Azhar Azhar; Ismail Ismail; Rastina Rastina; Eliawardani Eliawardani
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The objective of this research was to evaluate the anthelmintic activity of ethanolic extract of nuts Veitchia merrillii against intestinal nematode, Ascaridia galli. Phytochemical tests were performed for testing different chemicals group present in the extract. The effect of these extract were determined by in vitro based on inhibition of motility and mortality of worms. Amount of four worms were exposed in triplicate to each of phosphate buffered saline, 25 mg/mL, and 75 mg/mL crude ethanolic extract of V. merrillii, and 15 mg/mL albendazole. The motility of worm was observed on 9, 18, 27, and 36 hours interval. The mortality of worms were recognized by their straight flat appearance with no movements at the head and tail regions of the body. We found that the ethanolic extract of V. merrillii contains tannins, alkaloids, flavonoids, triterpenoids, and saponins neither steroid. Based on the in vitro trials conducted using above extract at 25 and 75 mg/mL concentration, the extract of V. merrillii showed anthelmintic effect. The extract of V. merrillii was effective at 75 mg/mL concentration only. The study indicated that it is potential to develop herbal-based anthelmintic to control A. galli in poutry.

Histopathological Changes of Egg Cells in the Uterine of Ascaridia galli after Treatment with Extract of Veitchia merrillii Nuts Ummu Balqis; Muslina Muslina; Muhammad Hambal; Farida Athaillah; Muttaqien Muttaqien; Azhar Azhar; Ismail Ismail; Rastina Rastina; Eliawardani Eliawardani; Abdul Harris; Abdullah Hamzah; Henni Vanda; Darmawi Darmawi
Majalah Obat Tradisional Vol 22, No 3 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The aim of the study was to observe the histopathological changes of the entire egg cells in the uterine of Ascaridia galli worms treated with crude ethanolic extract of Veitchia merrillii nuts. Histopathological changes of the entire egg cells were observed to describe that the activity of extract V. merrillii nuts interfered the reproduction process could decrease the quantity and quality of A. galli eggs. The experiments consisted of 16 head A. galli female adult worms divided into four groups, contained four worms of each, and maintained in 0.9% phosphate buffered saline (PBS). First group, a negative control experiment consisted of A. galli in 0.9% PBS. Second group, concentration of 15 mg/mL albendazole was used as a positive control. Third group and fourth group, in vitro exposure of the worms to the crude ethanolic extract V. merrillii nuts at a concentration of 25 mg/mL and 75 mg/mL, respectively. The histopathological changes of egg cells in the uterine region of stained worms was evaluated by light microscopic examination at 40x magnification. The results showed that shrinkage and disintegration of the entire egg cells in the breakage of uterus. The results indicate that the possible use of the plant as a potential anthelmintic against A. galli, the intestinal nematode parasite of domestic fowl.

Identifikasi Keberadaan Nyamuk Aedes Spp Vektor Demam Berdarah Dengue (DBD) Di Gampong Pineung Kecamatan Syiah Kuala Banda Aceh Dina Izzatina; Farida Athaillah; Muhammad Hanafiah; Lian Varis Riandi; Eliawardani Eliawardani; Winaruddin Winaruddin; Muttaqien Muttaqien; M. Isa
JURNAL ILMIAH MAHASISWA VETERINER Vol 7, No 1 (2022): NOVEMBER-JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v7i1.8751

ABSTRAKPenelitian ini bertujuan mengetahui keberadaan dan distribusi nyamuk Aedes spp sebagai vektor demam berdarah dengue (DBD) dengan menggunakan ovitrap di kawasan Gampong Pineung, Kecamatan Syiah Kuala baik didalam ruangan (indoor) maupun diluar ruangan (outdoor). Penelitian ini dilakukan di lima dusun di Gampong Pineung kecamatan Syiah Kuala yaitu: Tgk Chik Dipineung, Bintara Pineung, Tgk Hasyim, Tgk Tengoh, dan T Muda Rayeuk. Hasil pengamatan terhadap total rata-rata telur nyamuk Aedes spp di kelima dusun tidak memperlihatkan perberbedaan yang nyata (P0,05). Sedangkan jumlah total rata-rata telur nyamuk indoor dan outdoor antar dusun terdapat perbedaan yang nyata (P0,05). Tetapi pada pengamatan terhadap Jumlah rata-rata larva Aedes agypti pada indoor berbeda nyata (P0,05) dibandingkan dengan larva Aedes albopictus. Begitu pula dengan jumlah rata-rata larva Aedes agypti pada outdoor berbeda nyata (P0,05) dibandingkan dengan larva Aedes albopictus.Kata kunci : Ovitrap, Indoor, Outdoor dan Gampong Pineung.ABSTRACTThis study aims to determine the presence and distribution of Aedes spp mosquitoes as a vector of dengue hemorrhagic fever (DHF) using ovitrap in Gampong Pineung area, Syiah Kuala subdistrict both indoor (indoor) and outside (outdoor). The research was conducted in five sub-villages in Gampong Pineung, Syiah Kuala sub-district, namely: Tgk Chik Dipineung, Bintara Pineung, Tgk Hasyim, Tgk Tengoh, and T Muda Rayeuk. The observation of total Aedes spp mosquito eggs in the five hamlets did not show a significant difference (P 0.05). While the mean total number of indoor and outdoor mosquito eggs between hamlets there was a real difference (P 0,05). But on observation of the average number of Aedes agypti larvae on indoor space was significantly different (P 0.05) compared with Aedes albopictus larvae. Similarly, the average number of Aedes agypti larvae in outdoor spaces was significantly different (P 0.05) compared with the Aedes albopictus larvae.Keyword : Ovitrap , Indoor, Outdoor, and Gampong Pineung.

PROFIL DARAH TIKUS PUTIH (Rattus norvegicus) YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERIKAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) Yudha Fahrimal; Eliawardani E; Afira Rafina; Al Azhar; Nuzul Asmilia
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gambaran darah (hematokrit, eritrosit, leukosit, dan diferensial leukosit) tikus yang diinfeksi Trypanosoma evansi (T. evansi) dan diberi ekstrak kulit batang jaloh (Salix tetrasperma Roxb). Duapuluh lima ekor tikus jantan dibagi secara acak ke dalam 5 kelompok perlakuan yang masing-masing terdiri atas 5 ekor tikus. Kelompok 0 (K0) tanpa perlakuan, kelompok I (K1) hanya diinfeksikan dengan 103 T. evansi, kelompok II (K2) diinfeksikan dengan 103T. evansi dan diberikan ekstrak kulit batang jaloh 30 mg/kg bobot badan, kelompok III (K3) diinfeksikan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 45 mg/kg bobot badan, dan kelompok IV (K4) diinfeksi dengan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 60 mg/kg bobot badan. Infeksi T. evansi dilakukan secara intraperitoneal sedangkan ekstrak diberikan secara oral selama 3 hari berturut-turut. Hasil penelitian menunjukkan bahwa rata-rata ± SD nilai hematokrit dan eritrosit tikus dari K1, K2, K3 dan K4 lebih rendah dari K0. Sebaliknya, rata-rata ± SD jumlah leukosit (103/µl) lebih tinggi dari K0. Diferensial leukosit menunjukkan jumlah masing-masing sel leukosit semua tikus dalam kelompok perlakuan meningkat setelah pemberian ekstrak kulit batang jaloh kecuali eosinofil dan limfosit yang justru menurun. Dari penelitian dapat disimpulkan bahwa Infeksi T. evansi menurunkan kadar hematokrit dan eritrosit namun meningkatkan kadar leukosit tikus dan pemberian ekstrak kulit batang jaloh dosis rendah dalam waktu yang singkat mampu mengembalikan profil darah tikus mendekati nilai normal.

PROFIL DARAH TIKUS PUTIH (Rattus norvegicus) YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERIKAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) Yudha Fahrimal; Eliawardani E; Afira Rafina; Al Azhar; Nuzul Asmilia
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gambaran darah (hematokrit, eritrosit, leukosit, dan diferensial leukosit) tikus yang diinfeksi Trypanosoma evansi (T. evansi) dan diberi ekstrak kulit batang jaloh (Salix tetrasperma Roxb). Duapuluh lima ekor tikus jantan dibagi secara acak ke dalam 5 kelompok perlakuan yang masing-masing terdiri atas 5 ekor tikus. Kelompok 0 (K0) tanpa perlakuan, kelompok I (K1) hanya diinfeksikan dengan 103 T. evansi, kelompok II (K2) diinfeksikan dengan 103T. evansi dan diberikan ekstrak kulit batang jaloh 30 mg/kg bobot badan, kelompok III (K3) diinfeksikan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 45 mg/kg bobot badan, dan kelompok IV (K4) diinfeksi dengan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 60 mg/kg bobot badan. Infeksi T. evansi dilakukan secara intraperitoneal sedangkan ekstrak diberikan secara oral selama 3 hari berturut-turut. Hasil penelitian menunjukkan bahwa rata-rata ± SD nilai hematokrit dan eritrosit tikus dari K1, K2, K3 dan K4 lebih rendah dari K0. Sebaliknya, rata-rata ± SD jumlah leukosit (103/µl) lebih tinggi dari K0. Diferensial leukosit menunjukkan jumlah masing-masing sel leukosit semua tikus dalam kelompok perlakuan meningkat setelah pemberian ekstrak kulit batang jaloh kecuali eosinofil dan limfosit yang justru menurun. Dari penelitian dapat disimpulkan bahwa Infeksi T. evansi menurunkan kadar hematokrit dan eritrosit namun meningkatkan kadar leukosit tikus dan pemberian ekstrak kulit batang jaloh dosis rendah dalam waktu yang singkat mampu mengembalikan profil darah tikus mendekati nilai normal.

The Ectoparasites Inventory on Cultured Carp in Floating Cage Net at Laut Tawar Lake Kabupaten Aceh Tengah Winaruddin W; Eliawardani E
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

The aimed of this research is inventoried the parasites of carp in floating cage net at Laut TawarLake. This study used 100 fishes consisted of 50 fries and 50 reproductive stadia. The result of thisresearch indicates the parasites found in the fishes are Trichodina sp., Ichtyophthirius multifiliis,Dactylogyrus sp., Gyrodactylus sp., Epistylis sp. and Lernaea sp. Protozoan parasites were found in allfishes and infected fish organs, while metazoan parasites infected fins and gills and copepods only infectedskin.Keywords: parasites, carps, floating cage net, Laut Tawar Lake

8. Identification of Parasites on the Shark Fish (Selachimorpha) in Peunayong Fish Market Banda Aceh City Muttaqien Bakri; Iraidi Muharrir Asy’ari; Eliawardani Eliawardani; Muhammad Hambal; Winaruddin Winaruddin; Darmawi Darmawi; Azhari Azhari; Andi Novita
Jurnal Medika Veterinaria Vol 14, No 2 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i2.4480

This study aims to identify ectoparasites and endoparasites in the Peunayong Fish Market, Banda Aceh City. The number of shark samples used in this study was 10 which were taken randomly. Parasitic examination is carried out on the surface of the body by means of scraping and natively. While examination of the visceral organs of fish by necropsy. Parasite identification was done by microscopic. The results showed that the Chilodonella sp. parasite infected the stingrays of 10 sharks examined. The parasite species that infects sharks (Selachimorpha) in the Peunayong Fish Market in Banda Aceh City is Chilodonella sp, including the ectoparasite category that infects shark skin with a prevalence rate of 6.67%.

20. Ectoparasites Identification of Stingrays Fish (Dasyatis Sp.) At Peunayong Fish Market, Banda Aceh Muttaqien Muttaqien; Arini Ulfa Khaira; Winaruddin Winaruddin; Eliawardani Eliawardani; Muhammad Hambal; Al Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.3547

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages.The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks. The distribution ofHassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer

Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii) Ummu Balqis; Darmawi Darmawi; Maryam Maryam; Muslina Muslina; Abdullah Hamzah; Razali Daud; Muhammad Hambal; Rinidar Rinidar; Abdul Harris; Muttaqien Muttaqien; Azhar Azhar; Eliawardani Eliawardani
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v16i1.3022

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.(Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii)ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.

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