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. Darmawi
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Isolation of enterobacteriaceae bacteria species from feces of Sumatran Orangutans (Pongo abelii) . Darmawi; Erdiansyah Rahmi; Maryulia Dewi; Joharsyah Hutabarat; Mahdi Abrar; . Fakhrurrazi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively
The effect of excretory/secretory product released by L3 of A. galli on villous compact in intestine of laying hens Ummu Balqis; . Darmawi; Risa Tiuria; Bambang P. Priosoeryanto
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli.
Poultry sellers’ perception and their effort for prevention strategy on avian influenza in Banda Aceh, Indonesia . Darmawi; Teuku R. Ferasyi; . Muthmainnah; Ummu Balqis; . Fakhrurrazi; . Darniati; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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A study was conducted to examine some poultry sellers’ perception of the Avian influenza (birdflu) in nine locations (Ulee Kareng, Batoh, Lamnyong, Kampong Baru, Peunayong, Setui, Kampong Ateuk, and Neusu) in Banda Aceh Municipality, Indonesia. Primary data were obtained with the use of a structured questionnaire administared to ninety nine (99) respondents selected using proportional stratified random sampling, between November to December 2011. Information was obtained using the questionnaire sheets contain questions: knowladge, attitude, availability of facilities, and the effort of Avian influenza prevention strategy. Data obtained were analyzed by descriptive statistics. The result showed that only 52% of the poultry sellers’ had good knowledge of early symptomps of Avian influenza. Amount 59% of the poultry sellers’ had good attitudes. Result also showed that 73% of respondents had good facilities that was available in live poultry market area, but only 70% of respondents were good awareness about the effort of Avian influenza prevention strategy. In addition, the respondents who had more knowledge, good attitudes, and/or good facilities were also those who actually acted more preventively. Based on the result we concluded that the poultry sellers’ perception on prevention strategy for infection of birdflu in Banda Aceh categorized as poor. It is suggested that compliance with preventive measures may be enhanced through behavioural modification and building awareness through communication, training, workshop programs to the live poultry market sellers’ in Banda Aceh
Co-Authors . Darniati . Fakhrurrazi . Muthmainnah Bambang P. Priosoeryanto Erdiansyah Rahmi Joharsyah Hutabarat Mahdi Abrar Maryulia Dewi Muhammad Hambal Risa Tiuria Teuku R. Ferasyi Ummu Balqis