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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal TEMAPELA Jurnal Medika Veterinaria
Maryulia Dewi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Isolation of enterobacteriaceae bacteria species from feces of Sumatran Orangutans (Pongo abelii) . Darmawi; Erdiansyah Rahmi; Maryulia Dewi; Joharsyah Hutabarat; Mahdi Abrar; . Fakhrurrazi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.129 KB)

Abstract

The porpouse of this research aimed to isolate enterobacteriaceae bacteria from feces of Pongo abelii. The samples of feces were collected from 15 captive orangutans in Orangutan Sumatera Batu Mbelin Sibolangit Qurantine, North Sumatra. Of each sample was cultured in nutrient broth media using sterilecotton swabs or Pasteur pipettes, and incubated at 37°C temperature for 24 hours. Culture was spared on Methylene Blue Agar (Oxoid), examined by Gram staining, and tested by biochemically. The result showed that significantly more common appear Escherichia sp. (93,33%) and fewer Edwardsiella sp. (66,67%) wereisolated from feces samples of P. abelii. Others enterobacteriaceae found in feces of P. Abelii were Shigella sp. (46,67%), Klebsiella sp. (33,33%), Citrobacter sp., and Salmonella sp. (13,33%), respectively
Pengujian Hidrofobisitas dan Aktivitas Antibiotik terhadap Staphylococcus aureus Isolat Preputium Sapi Aceh Maryulia Dewi; Darmawi T; Zahrial Helmi
Jurnal Teknologi dan Manajemen Pengelolaan Laboratorium Vol 1 No 2 (2018): Jurnal Teknologi dan Manajemen Pengelolaan Laboratorium (TEMAPELA)
Publisher : Labor Dasar dan Sentral Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.739 KB) | DOI: 10.25077/temapela.1.2.72-75.2018

Abstract

Penelitian ini bertujuan untuk melihat karaksteristik dan aktivitas antibiotik terhadap hidrofobisitas Stapylococcus aureus isolat preputium sapi Aceh menggunakan hidrokarbon xylol dan pembaca ELLISA. Hidrofobisitas permukaan berbagai sel mikroba dapat ditentukan dengan mengukur kemampuan penempelan sel terhadap berbagai polimer atau afinitas bakteri terhadap pelarut hidrokarbon. Pengujian hidrofobisitas bakteri dilakukan pada media nutrien cair dengan modifikasi hidrokarbon xylol, menggunakan mikroplat U-bottom polystyrene 96 wells. Antibiotik yang digunakan adalah tetrasiklin, oksitetrasiklin dan fosfomisin dengan konsentrasi masing-masing 10%, 20% dan 30% (mg/mL). OD hidrofobisitas dibaca pada λ 600 nm menggunakan pembaca ELISSA. Data hasil penelitian di analisis menggunakan analysis of variance (ANOVA). Hasil penelitian menunjukkan aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik hidrofobisitas tergolong positif moderat, yaitu 36,76%. Perlakuan antibiotik berpengaruh secara nyata (P<0,05) terhadap daya hidrofobisitas, sedangkan konsentrasi tidak berpengaruh secara nyata (p<0,05) terhadap daya hidrofobisitas Staphylococcus aureus isolat preputium sapi Aceh. Tetrasiklin 30% (mg/mL) mempunyai kemampuan paling baik meningkatkan hidrofobisitas Staphylococcus aureus isolat preputium sapi Aceh sebesar 36,16% menjadi 72,29%, sehingga aktivitas virulensi Staphylococcus aureus isolat preputium sapi Aceh berdasarkan karaksteristik hidrofobisitas dari positif moderat menjadi positif palsu.
CEMARAN Staphylococcus aureus PADA DAGING SAPI YANG DIPASARKAN DI PASAR TRADISIONAL KOTA BANDA ACEH (Staphylococcus aureus Contamination in Beef from Traditional Markets in Banda Aceh) Jhon Leo Danubrata; Fakhrurrazi Fakhrurrazi; Maryulia Dewi; Sulasmi Sulasmi; Faisal Jamin; T. Armansyah TR; Nurliana Nurliana
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3813

Abstract

This research aimed to determine the total amount of Staphylococcus aureus (S. aureus) contamination in beef sold in traditional market in Banda Aceh. Total of 16 beef samples were used in this research, 2 samples from Beurawe market, 10 samples from Peunayong market, and 4 samples from Seutui market. Samples were cultured on manitol salt agar. The number of S. aureus colonies was calculated using colony counter, and the result was compared to the limit of microbial contamination based on Indonesian National Standard (SNI). The result of this research showed that the highest level of S. aureus contamination was derived from Beurawe market. It is concluded that all beef sold by seller from traditional market in Banda Aceh were contaminated by S. aureus.Key words: Staphylococcus aureus, meat, traditional market, number of contamination
2. Antibacterial Test of Red Dragon Fruit Extract Peel (Hylocereus polyrhizus) Against Bacteria Salmonella pullorum Ichsan Maulana; Abdul Harris; Fakhrurrazi Fakhrurrazi; Maryulia Dewi; Safika Safika; Erina Erina; Muhammad Jalaluddin
Jurnal Medika Veterinaria Vol 12, No 1 (2018): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v12i1.4241

Abstract

The purpose of this study is to determine the effect of the red dragon fruit peels (Hylocereus polyrhizus) extract on the growth of Salmonella pullorum. This research was conducted at the Laboratory of Microbiology, Veterinary Faculty, University of Syiah Kuala, Banda Aceh. Red dragon fruit peels that used in this study is obtained from  ripe fruit and Salmonella pullorum cultures is  obtained from  Microbiology Laboratory of Veterinary Faculty Unsyiah. Salmonella pullorum culture added evenly on the surface of Mueller Hinton Agar, the number of the bacteria have been standarized using Mc Farland'1 method. Discs soaked in the red dragon fruit peels extracts at different concentrations (20 mg/ml, 40 mg/mL, and 60 mg/ml). Ampicillin is used as positive controls and DMSO (dimethyl sulfoxide) was placed on the surface of Mueller Hinton Agar. The parameters in this study is the inhibition zone diameter. Data were analyzed descriptively. The results showed that the red dragon fruit peel extract is able to inhibit the growth of Salmonella  pullorum and the average of  inhibition diameter zone at a concentration 60 mg / ml is 9.6 mm, 40 mg / ml is 9.4 mm and 20 mg / ml is 9.3 mm
5. Isolation and Identification of Salmonella sp in spleen of male layer chicken in Sibreh farms, Aceh Besar Erina Erina; Mahdi Abrar; Balqis Alrasyid Suyoto; Maryulia Dewi; Darmawi Darmawi; Darniati Darniati; Muttaqien Bakri
Jurnal Medika Veterinaria Vol 11, No 1 (2017): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v11i1.3986

Abstract

This study aimed to isolate and identify the bacteria Salmonella sp in spleen of male layer chicken that died in farms Sibreh, Aceh Besar. A sample of the research is the spleen of male layer chickens. Isolation of Salmonella sp performed by inoculating spleen that were pierced using a swab on the media Selenite Cystine Broth (SCB) then planted on selective media Salmonella Shigella Agar (SSA) and colony morphology was observed, continued with observation of Gram stain under microscopic. Furthermore, colonies of suspected Salmonella sp planted in the media MR-VP (Oxoid), Indol (Difco), Simmons's Citrate Agar (Oxoid), Triple Sugar Iron Agar (TSIA), Sulfite Indol Motiliti (SIM), and media sugars (sucrose, manitol, glucose, lactose , maltose). The results of this study were analyzed descriptively. The research shows a results from a total of 33 samples, we found that 15 samples are positive that can be isolated and identified as Salmonella sp. Therefore it can be concluded that Salmonella sp is possible the death cause of male layer chicken in farms Sibreh, Aceh Besar
10. Isolation and identification of Aeromonas sp in goldfish (Carrasius auratus) from several pet shops in Banda Aceh Hamny Hamny; Muhammad Zulfan; Mahdi Abrar; Maryulia Dewi; Darmawi Darmawi; Razali Daud; Budianto Panjaitan
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4225

Abstract

The aim of this study was to isolate and identify Aeromonas sp from pet shops in Banda Aceh. A total of 15 goldfish were obtained from 3 pet shop in Banda Aceh and examined at the Laboratory of Microbiology, Faculty of Veterinary Medicine, University of Syiah Kuala in Banda Aceh. Isolation of bacteria obtained from liver, kidneys and spleen were cultured in the media Rimler-Shotts (RS) Medium Base. The bacteria were identified based on morphology of the growing bacteria, Gram stain, IMViC test, and biochemical tests. The results showed that there were no Aeromonas sp in goldfish (Carrasius auratus) from three pet shops in Banda Aceh.
INFEKSI BAKTERI Escherichia coli PADA ANAK AYAM KAMPUNG (Gallus domesticus) DI PASAR LAMBARO ACEH BESAR Faisal Jamin; Mahdi Abrar; Maryulia Dewi; Yanrivina S.V.S; Fakhrurrazi -; Zakiah Heryawaty Manaf; Syafruddin -
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2998

Abstract

Penelitian ini bertujuan mengetahui infeksi bakteri Escherichia coli pada anak ayam kampung (Gallus domesticus). Sampel yang digunakan dalam penelitian ini adalah feses anak ayam kampung yang diperoleh dari Pasar Lambaro, Aceh Besar. Sampel diambil dengan cara swab bagian kloaka anak ayam dengan menggunakan swab steril, kemudian sampel dimasukkan ke dalam microtube, dan dibawa ke laboratorium untuk dilakukan uji selanjutnya. Penelitian ini dilakukan dengan tiga kali pengambilan sampel dengan jangka waktu satu minggu dengan jumlah sampel uji sebanyak 15 sampel. Pengambilan sampel dilakukan dengan menggunakan metode Carter. Data yang diperoleh dianalisis secara deskriptif. Hasil pemeriksaan bakteri Escherichia coli (E. coli) yang telah dilakukan pada 15 sampel diketahui bahwa 9 sampel positif terinfeksi bakteri E. coli sedangkan 6 sampel lain positif bakteri coli form lainnya. Disimpulkan bahwa bakteri E. coli menginfeksi anak ayam kampung di Pasar Lambaro Aceh Besar.
6. Isolation, Identification and Sensitivity Test of Staphylococcus aureus on Post Surgery Wound of Local Dogs (Canis familiaris) Darmawi Darmawi; Amalia Fatma Zahra; M. Nur Salim; Maryulia Dewi; Mahdi Abrar; Syafruddin Syafruddin; Mulyadi Adam
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i1.4122

Abstract

This study aimed to isolate, identify, and sensitivity tested of Staphylococcus aureus from post-surgery wound of local dogs.This study was held in Microbiology Laboratorium of Veterinary Faculty of Syiah Kuala University, Banda Aceh from April to May 2016. The samples were 10 dogs in their 3rd day’s treatment after surgery.The samples were acquired using sterile swab on the wound that was planted on Nutrient Broth (NB), incubated at 37°C for 24 hours, then transferred onto Mannitol Salt Agar (MSA), incubated at 37°C for 24 hours, and after that it was identified with Gram staining, catalase test, sugar test (mannitol and glucose), Metil Red-Voges Proskauer (MR-VP) test and antibiotic sensitivity test. The result of   Gram staining showed 7 positive results which identified as coccus formed and coloured as purple. The catalase test showed 7 positive results, the sugar test (mannitol and glucose) showed 4 positive results, the MR-VP showed 4 positive on MR and all negative on VP.. In conclusion, 4 out of 10 samples showed a presence of Staphylococcus aureus on post-surgery wound of local dogs.This bacterium was sensitive on Tetracycline (50%) and also on Streptomycine and Gentamicin (100%). This bacterium resistant to  Tetracycline (50%) and Ampicillin (100%).
DETEKSI ANTIBODI SERUM AYAM KAMPUNG (Gallus domesticus) TERHADAP VIRUS NEWCASTLE DISEASE DI KOTA BANDA ACEH Darmawi -; Fakhrurrazi -; Wiliana -; Maryulia Dewi; Mahdi Abrar; Faisal Jamin; Zakiah Heryawati Manaf
Jurnal Medika Veterinaria Vol 9, No 1 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i1.2983

Abstract

Penelitian ini bertujuan mengetahui titer antibodi pada serum ayam kampung (Gallus domesticus) terhadap virus Newcastle disease (ND). Sampel penelitian adalah serum ayam kampung yang diperoleh di beberapa pasar tradisional Banda Aceh. Parameter yang diamati dalam penelitian ini adalah titer antibodi serum ayam kampung dari uji hemaglutinasi inhibisi (HI) dengan memperhatikan endapan eritrosit. Data hasil pengamatan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa dari 120 sampel yang diambil di beberapa pasar tradisional di kota Banda Aceh hanya 15 sampel (12,5%) menunjukkan protektif sedangkan 105 sampel (87,5%) tidak protektif. Tingkat proteksi ayam terhadap virus ND di pasar tradisional di kota Banda Aceh tergolong sangat rendah.
Co-Authors . Darmawi . Darniati . Fakhrurrazi Abdul Harris Amalia Fatma Zahra Aminuyati Balqis Alrasyid Suyoto Budianto Panjaitan Darmawi - Darmawi Darmawi Darmawi Darmawi Darmawi T Erdiansyah Rahmi Erina Erina Faisal Jamin Fakhrurrazi - Fakhrurrazi Fakhrurrazi Hamny Sofyan Ichsan Maulana Jhon Leo Danubrata Joharsyah Hutabarat M. Nur Salim Mahdi Abrar Mahdi Abrar Muhammad Jalaluddin Muhammad Zulfan Mulyadi Adam Muttaqien Bakri NURLIANA NURLIANA Razali Daud Safika S, Safika Sulasmi Sulasmi Syafruddin - Syafruddin Syafruddin T. Armansyah TR Wiliana - Yanrivina S.V.S Zahrial Helmi Zakiah Heryawati Manaf Zakiah Heryawaty Manaf